UniProtKB - Q3UR32 (P2RX3_MOUSE)
Protein
P2X purinoceptor 3
Gene
P2rx3
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for ATP that acts as a ligand-gated cation channel (By similarity). Plays a role in sensory perception (PubMed:15961431, PubMed:16322458). Required for normal perception of pain (PubMed:15961431). Required for normal taste perception (PubMed:16322458).By similarity2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 172 | ATPBy similarity | 1 | |
Binding sitei | 275 | ATPBy similarity | 1 | |
Binding sitei | 299 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 63 – 65 | ATPBy similarity | 3 | |
Nucleotide bindingi | 279 – 281 | ATPBy similarity | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- extracellularly ATP-gated cation channel activity Source: MGI
- identical protein binding Source: MGI
- purinergic nucleotide receptor activity Source: UniProtKB
GO - Biological processi
- behavioral response to formalin induced pain Source: MGI
- behavioral response to pain Source: MGI
- cation transport Source: MGI
- cellular response to ATP Source: UniProtKB
- chemical synaptic transmission Source: MGI
- inorganic cation transmembrane transport Source: UniProtKB
- ion transport Source: MGI
- modulation of chemical synaptic transmission Source: SynGO
- neuromuscular synaptic transmission Source: MGI
- neuronal action potential Source: MGI
- peristalsis Source: MGI
- positive regulation of sensory perception of pain Source: MGI
- protein homotrimerization Source: UniProtKB
- regulation of synaptic plasticity Source: MGI
- response to ATP Source: MGI
- response to carbohydrate Source: MGI
- response to cold Source: MGI
- response to heat Source: MGI
- response to hypoxia Source: MGI
- response to mechanical stimulus Source: MGI
- response to organic substance Source: MGI
- response to temperature stimulus Source: MGI
- sensory perception of taste Source: MGI
- urinary bladder smooth muscle contraction Source: MGI
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-139853, Elevation of cytosolic Ca2+ levels R-MMU-418346, Platelet homeostasis |
Names & Taxonomyi
Protein namesi | Recommended name: P2X purinoceptor 3Short name: P2X3 Alternative name(s): ATP receptor Purinergic receptor |
Gene namesi | Name:P2rx3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1097160, P2rx3 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- rough endoplasmic reticulum Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Nucleus
- integral component of nuclear inner membrane Source: GO_Central
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- integral component of presynaptic membrane Source: MGI
Other locations
- axon Source: MGI
- dendritic spine Source: MGI
- hippocampal mossy fiber to CA3 synapse Source: SynGO
- membrane Source: MGI
- membrane raft Source: MGI
- neuron projection Source: MGI
- neuronal cell body Source: MGI
- receptor complex Source: MGI
- Schaffer collateral - CA1 synapse Source: SynGO
- terminal bouton Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 20 | CytoplasmicCuratedAdd BLAST | 20 | |
Transmembranei | 21 – 43 | Helical; Name=1By similarityAdd BLAST | 23 | |
Topological domaini | 44 – 322 | ExtracellularCuratedAdd BLAST | 279 | |
Transmembranei | 323 – 341 | Helical; Name=2By similarityAdd BLAST | 19 | |
Topological domaini | 342 – 397 | CytoplasmicCuratedAdd BLAST | 56 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Simultaneous knockout of P2rx2 and P2rx3 results in reduced pain-related behaviors in response to intraplantar injection of formalin and reduced urinary bladder reflexes and decreased pelvic afferent nerve activity in response to bladder distension. Neurons have minimal to no response to ATP (PubMed:15961431). Simultaneous knockout of P2rx2 and P2rx3 results in defects in taste responses in the taste nerves and reduced behavioral responses to sweeteners, glutamate and bitter substances (PubMed:16322458).2 Publications
Chemistry databases
ChEMBLi | CHEMBL2401608 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000269195 | 1 – 397 | P2X purinoceptor 3Add BLAST | 397 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 107 ↔ 153 | By similarity | ||
Disulfide bondi | 116 ↔ 137 | By similarity | ||
Disulfide bondi | 122 ↔ 147 | By similarity | ||
Glycosylationi | 139 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 170 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 194 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 203 ↔ 213 | By similarity | ||
Disulfide bondi | 247 ↔ 256 | By similarity | ||
Glycosylationi | 290 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | Q3UR32 |
PaxDbi | Q3UR32 |
PRIDEi | Q3UR32 |
ProteomicsDBi | 294288 |
PTM databases
GlyGeni | Q3UR32, 4 sites |
iPTMneti | Q3UR32 |
PhosphoSitePlusi | Q3UR32 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000027071, Expressed in dorsal root ganglion and 75 other tissues |
ExpressionAtlasi | Q3UR32, baseline and differential |
Genevisiblei | Q3UR32, MM |
Interactioni
Subunit structurei
Homotrimer (By similarity). Functional P2XRs are organized as homomeric and heteromeric trimers (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000028465 |
Miscellaneous databases
RNActi | Q3UR32, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the P2X receptor family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QUDE, Eukaryota |
GeneTreei | ENSGT01020000230537 |
InParanoidi | Q3UR32 |
OMAi | VQGFCPE |
OrthoDBi | 1128763at2759 |
PhylomeDBi | Q3UR32 |
TreeFami | TF328633 |
Family and domain databases
Gene3Di | 2.60.490.10, 1 hit |
InterProi | View protein in InterPro IPR003046, P2X3_purnocptor IPR027309, P2X_extracellular_dom_sf IPR001429, P2X_purnocptor |
PANTHERi | PTHR10125:SF8, PTHR10125:SF8, 1 hit |
PIRSFi | PIRSF005713, P2X_purinoceptor, 1 hit |
PRINTSi | PR01310, P2X3RECEPTOR PR01307, P2XRECEPTOR |
TIGRFAMsi | TIGR00863, P2X, 1 hit |
PROSITEi | View protein in PROSITE PS01212, P2X_RECEPTOR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q3UR32-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNCISDFFTY ETTKSVVVKS WTIGIINRAV QLLIISYFVG WVFLHEKAYQ
60 70 80 90 100
VRDTAIESSV VTKVKGFGRY ANRVMDVSDY VTPPQGTSVF VIITKMIVTE
110 120 130 140 150
NQMQGFCPEN EEKYRCVSDS QCGPERFPGG GILTGRCVNY SSVRRTCEIQ
160 170 180 190 200
GWCPTEVDTV EMPIMMEAEN FTIFIKNSIR FPLFNFEKGN LLPNLTDKDI
210 220 230 240 250
KKCRFHPEKA PFCPILRVGD VVKFAGQDFA KLARTGGVLG IKIGWVCDLD
260 270 280 290 300
KAWDQCIPKY SFTRLDGVSE KSSVSPGYNF RFAKYYKMEN GSEYRTLLKA
310 320 330 340 350
FGIRFDVLVY GNAGKFNIIP TIISSVAAFT SVGVGTVLCD IILLNFLKGA
360 370 380 390
DHYKARKFEE VTETTLKGTA STNPVFTSDQ ATVEKQSTDS GAYSIGH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AW04 | A2AW04_MOUSE | P2X purinoceptor | P2rx3 | 395 | Annotation score: | ||
A2AW03 | A2AW03_MOUSE | P2X purinoceptor | P2rx3 | 373 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 295 | R → P in AAH23089 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK140283 mRNA Translation: BAE24316.1 AK141847 mRNA Translation: BAE24856.1 AL935159 Genomic DNA No translation available. BC023089 mRNA Translation: AAH23089.1 |
CCDSi | CCDS16200.1 |
RefSeqi | NP_663501.2, NM_145526.2 |
Genome annotation databases
Ensembli | ENSMUST00000028465; ENSMUSP00000028465; ENSMUSG00000027071 |
GeneIDi | 228139 |
KEGGi | mmu:228139 |
UCSCi | uc008kjr.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK140283 mRNA Translation: BAE24316.1 AK141847 mRNA Translation: BAE24856.1 AL935159 Genomic DNA No translation available. BC023089 mRNA Translation: AAH23089.1 |
CCDSi | CCDS16200.1 |
RefSeqi | NP_663501.2, NM_145526.2 |
3D structure databases
SMRi | Q3UR32 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000028465 |
Chemistry databases
ChEMBLi | CHEMBL2401608 |
PTM databases
GlyGeni | Q3UR32, 4 sites |
iPTMneti | Q3UR32 |
PhosphoSitePlusi | Q3UR32 |
Proteomic databases
MaxQBi | Q3UR32 |
PaxDbi | Q3UR32 |
PRIDEi | Q3UR32 |
ProteomicsDBi | 294288 |
Protocols and materials databases
Antibodypediai | 14096, 240 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028465; ENSMUSP00000028465; ENSMUSG00000027071 |
GeneIDi | 228139 |
KEGGi | mmu:228139 |
UCSCi | uc008kjr.1, mouse |
Organism-specific databases
CTDi | 5024 |
MGIi | MGI:1097160, P2rx3 |
Phylogenomic databases
eggNOGi | ENOG502QUDE, Eukaryota |
GeneTreei | ENSGT01020000230537 |
InParanoidi | Q3UR32 |
OMAi | VQGFCPE |
OrthoDBi | 1128763at2759 |
PhylomeDBi | Q3UR32 |
TreeFami | TF328633 |
Enzyme and pathway databases
Reactomei | R-MMU-139853, Elevation of cytosolic Ca2+ levels R-MMU-418346, Platelet homeostasis |
Miscellaneous databases
BioGRID-ORCSi | 228139, 0 hits in 52 CRISPR screens |
ChiTaRSi | P2rx3, mouse |
PROi | PR:Q3UR32 |
RNActi | Q3UR32, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027071, Expressed in dorsal root ganglion and 75 other tissues |
ExpressionAtlasi | Q3UR32, baseline and differential |
Genevisiblei | Q3UR32, MM |
Family and domain databases
Gene3Di | 2.60.490.10, 1 hit |
InterProi | View protein in InterPro IPR003046, P2X3_purnocptor IPR027309, P2X_extracellular_dom_sf IPR001429, P2X_purnocptor |
PANTHERi | PTHR10125:SF8, PTHR10125:SF8, 1 hit |
PIRSFi | PIRSF005713, P2X_purinoceptor, 1 hit |
PRINTSi | PR01310, P2X3RECEPTOR PR01307, P2XRECEPTOR |
TIGRFAMsi | TIGR00863, P2X, 1 hit |
PROSITEi | View protein in PROSITE PS01212, P2X_RECEPTOR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | P2RX3_MOUSE | |
Accessioni | Q3UR32Primary (citable) accession number: Q3UR32 Secondary accession number(s): A2AW02, Q8R1U4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 12, 2006 |
Last sequence update: | October 11, 2005 | |
Last modified: | April 7, 2021 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families