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Protein

P2X purinoceptor 3

Gene

P2rx3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Receptor for ATP that acts as a ligand-gated cation channel (By similarity). Plays a role in sensory perception (PubMed:15961431, PubMed:16322458). Required for normal perception of pain (PubMed:15961431). Required for normal taste perception (PubMed:16322458).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei172ATPBy similarity1
Binding sitei275ATPBy similarity1
Binding sitei299ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi63 – 65ATPBy similarity3
Nucleotide bindingi279 – 281ATPBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • ATP-gated ion channel activity Source: GO_Central
  • extracellularly ATP-gated cation channel activity Source: MGI
  • purinergic nucleotide receptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-139853 Elevation of cytosolic Ca2+ levels
R-MMU-418346 Platelet homeostasis

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 3
Short name:
P2X3
Alternative name(s):
ATP receptor
Purinergic receptor
Gene namesi
Name:P2rx3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1097160 P2rx3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicCuratedAdd BLAST20
Transmembranei21 – 43Helical; Name=1By similarityAdd BLAST23
Topological domaini44 – 322ExtracellularCuratedAdd BLAST279
Transmembranei323 – 341Helical; Name=2By similarityAdd BLAST19
Topological domaini342 – 397CytoplasmicCuratedAdd BLAST56

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Simultaneous knockout of P2rx2 and P2rx3 results in reduced pain-related behaviors in response to intraplantar injection of formalin and reduced urinary bladder reflexes and decreased pelvic afferent nerve activity in response to bladder distension. Neurons have minimal to no response to ATP (PubMed:15961431). Simultaneous knockout of P2rx2 and P2rx3 results in defects in taste responses in the taste nerves and reduced behavioral responses to sweeteners, glutamate and bitter substances (PubMed:16322458).2 Publications

Chemistry databases

ChEMBLiCHEMBL2401608

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002691951 – 397P2X purinoceptor 3Add BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi107 ↔ 153By similarity
Disulfide bondi116 ↔ 137By similarity
Disulfide bondi122 ↔ 147By similarity
Glycosylationi139N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi194N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi203 ↔ 213By similarity
Disulfide bondi247 ↔ 256By similarity
Glycosylationi290N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ3UR32
PaxDbiQ3UR32
PRIDEiQ3UR32

PTM databases

iPTMnetiQ3UR32
PhosphoSitePlusiQ3UR32

Expressioni

Gene expression databases

BgeeiENSMUSG00000027071 Expressed in 41 organ(s), highest expression level in dorsal root ganglion
ExpressionAtlasiQ3UR32 baseline and differential
GenevisibleiQ3UR32 MM

Interactioni

Subunit structurei

Homotrimer (By similarity). Functional P2XRs are organized as homomeric and heteromeric trimers (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028465

Structurei

3D structure databases

ProteinModelPortaliQ3UR32
SMRiQ3UR32
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFJF Eukaryota
ENOG410XR0C LUCA
GeneTreeiENSGT00390000016028
HOGENOMiHOG000232042
HOVERGENiHBG053086
InParanoidiQ3UR32
KOiK05217
OrthoDBiEOG091G0A2U
PhylomeDBiQ3UR32
TreeFamiTF328633

Family and domain databases

Gene3Di2.60.490.10, 1 hit
InterProiView protein in InterPro
IPR003046 P2X3_purnocptor
IPR027309 P2X_extracellular_dom_sf
IPR001429 P2X_purnocptor
PANTHERiPTHR10125 PTHR10125, 1 hit
PTHR10125:SF8 PTHR10125:SF8, 1 hit
PfamiView protein in Pfam
PF00864 P2X_receptor, 1 hit
PIRSFiPIRSF005713 P2X_purinoceptor, 1 hit
PRINTSiPR01310 P2X3RECEPTOR
PR01307 P2XRECEPTOR
TIGRFAMsiTIGR00863 P2X, 1 hit
PROSITEiView protein in PROSITE
PS01212 P2X_RECEPTOR, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q3UR32-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNCISDFFTY ETTKSVVVKS WTIGIINRAV QLLIISYFVG WVFLHEKAYQ
60 70 80 90 100
VRDTAIESSV VTKVKGFGRY ANRVMDVSDY VTPPQGTSVF VIITKMIVTE
110 120 130 140 150
NQMQGFCPEN EEKYRCVSDS QCGPERFPGG GILTGRCVNY SSVRRTCEIQ
160 170 180 190 200
GWCPTEVDTV EMPIMMEAEN FTIFIKNSIR FPLFNFEKGN LLPNLTDKDI
210 220 230 240 250
KKCRFHPEKA PFCPILRVGD VVKFAGQDFA KLARTGGVLG IKIGWVCDLD
260 270 280 290 300
KAWDQCIPKY SFTRLDGVSE KSSVSPGYNF RFAKYYKMEN GSEYRTLLKA
310 320 330 340 350
FGIRFDVLVY GNAGKFNIIP TIISSVAAFT SVGVGTVLCD IILLNFLKGA
360 370 380 390
DHYKARKFEE VTETTLKGTA STNPVFTSDQ ATVEKQSTDS GAYSIGH
Length:397
Mass (Da):44,437
Last modified:October 11, 2005 - v1
Checksum:iBFB90C740E5DD910
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AW03A2AW03_MOUSE
p2X purinoceptor
P2rx3
373Annotation score:
A2AW04A2AW04_MOUSE
p2X purinoceptor
P2rx3
395Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti295R → P in AAH23089 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK140283 mRNA Translation: BAE24316.1
AK141847 mRNA Translation: BAE24856.1
AL935159 Genomic DNA No translation available.
BC023089 mRNA Translation: AAH23089.1
CCDSiCCDS16200.1
RefSeqiNP_663501.2, NM_145526.2
UniGeneiMm.440375
Mm.448935

Genome annotation databases

EnsembliENSMUST00000028465; ENSMUSP00000028465; ENSMUSG00000027071
GeneIDi228139
KEGGimmu:228139
UCSCiuc008kjr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK140283 mRNA Translation: BAE24316.1
AK141847 mRNA Translation: BAE24856.1
AL935159 Genomic DNA No translation available.
BC023089 mRNA Translation: AAH23089.1
CCDSiCCDS16200.1
RefSeqiNP_663501.2, NM_145526.2
UniGeneiMm.440375
Mm.448935

3D structure databases

ProteinModelPortaliQ3UR32
SMRiQ3UR32
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028465

Chemistry databases

ChEMBLiCHEMBL2401608

PTM databases

iPTMnetiQ3UR32
PhosphoSitePlusiQ3UR32

Proteomic databases

MaxQBiQ3UR32
PaxDbiQ3UR32
PRIDEiQ3UR32

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028465; ENSMUSP00000028465; ENSMUSG00000027071
GeneIDi228139
KEGGimmu:228139
UCSCiuc008kjr.1 mouse

Organism-specific databases

CTDi5024
MGIiMGI:1097160 P2rx3

Phylogenomic databases

eggNOGiENOG410IFJF Eukaryota
ENOG410XR0C LUCA
GeneTreeiENSGT00390000016028
HOGENOMiHOG000232042
HOVERGENiHBG053086
InParanoidiQ3UR32
KOiK05217
OrthoDBiEOG091G0A2U
PhylomeDBiQ3UR32
TreeFamiTF328633

Enzyme and pathway databases

ReactomeiR-MMU-139853 Elevation of cytosolic Ca2+ levels
R-MMU-418346 Platelet homeostasis

Miscellaneous databases

PROiPR:Q3UR32
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027071 Expressed in 41 organ(s), highest expression level in dorsal root ganglion
ExpressionAtlasiQ3UR32 baseline and differential
GenevisibleiQ3UR32 MM

Family and domain databases

Gene3Di2.60.490.10, 1 hit
InterProiView protein in InterPro
IPR003046 P2X3_purnocptor
IPR027309 P2X_extracellular_dom_sf
IPR001429 P2X_purnocptor
PANTHERiPTHR10125 PTHR10125, 1 hit
PTHR10125:SF8 PTHR10125:SF8, 1 hit
PfamiView protein in Pfam
PF00864 P2X_receptor, 1 hit
PIRSFiPIRSF005713 P2X_purinoceptor, 1 hit
PRINTSiPR01310 P2X3RECEPTOR
PR01307 P2XRECEPTOR
TIGRFAMsiTIGR00863 P2X, 1 hit
PROSITEiView protein in PROSITE
PS01212 P2X_RECEPTOR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiP2RX3_MOUSE
AccessioniPrimary (citable) accession number: Q3UR32
Secondary accession number(s): A2AW02, Q8R1U4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 11, 2005
Last modified: November 7, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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