UniProtKB - Q3UQ28 (PXDN_MOUSE)
Protein
Peroxidasin homolog
Gene
Pxdn
Organism
Mus musculus (Mouse)
Status
Functioni
Displays low peroxidase activity and is likely to participate in H2O2 metabolism and peroxidative reactions in the cardiovascular system (By similarity). Plays a role in extracellular matrix formation.By similarity
Catalytic activityi
- EC:1.11.1.7
Cofactori
Protein has several cofactor binding sites:- Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
- heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group covalently per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 823 | Heme; covalent, via 2 linksBy similarity | 1 | |
Active sitei | 824 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 825 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 904 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 906 | Calcium; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 908 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 910 | CalciumPROSITE-ProRule annotation | 1 | |
Sitei | 974 | Transition state stabilizerPROSITE-ProRule annotation | 1 | |
Binding sitei | 977 | Heme; covalent, via 2 linksBy similarity | 1 | |
Metal bindingi | 1071 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- bromide peroxidase activity Source: MGI
- extracellular matrix structural constituent Source: MGI
- heme binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- peroxidase activity Source: UniProtKB
GO - Biological processi
- collagen fibril organization Source: MGI
- extracellular matrix organization Source: UniProtKB
- hydrogen peroxide catabolic process Source: UniProtKB
- response to oxidative stress Source: InterPro
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Biological process | Hydrogen peroxide |
Ligand | Calcium, Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2243919, Crosslinking of collagen fibrils |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Pxdn Synonyms:Kiaa0230 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1916925, Pxdn |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix 1 Publication
Note: Enriched in the peritubular space of fibrotic kidneys.
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Extracellular region or secreted
- extracellular space Source: BHF-UCL
Other locations
- collagen-containing extracellular matrix Source: UniProtKB
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000319620 | 24 – 1475 | Peroxidasin homologAdd BLAST | 1452 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 264 ↔ 314 | By similarity | ||
Disulfide bondi | 360 ↔ 409 | By similarity | ||
Disulfide bondi | 451 ↔ 499 | By similarity | ||
Disulfide bondi | 543 ↔ 591 | By similarity | ||
Glycosylationi | 637 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 696 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 716 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 728 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 729 ↔ 745 | By similarity | ||
Disulfide bondi | 844 ↔ 854 | By similarity | ||
Disulfide bondi | 848 ↔ 872 | By similarity | ||
Disulfide bondi | 956 ↔ 967 | By similarity | ||
Modified residuei | 1173 | PhosphotyrosineBy similarity | 1 | |
Disulfide bondi | 1174 ↔ 1231 | By similarity | ||
Glycosylationi | 1175 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 1177 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 1272 ↔ 1298 | By similarity | ||
Glycosylationi | 1277 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1364 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | Q3UQ28 |
PaxDbi | Q3UQ28 |
PRIDEi | Q3UQ28 |
ProteomicsDBi | 300361 |
PTM databases
GlyConnecti | 2580, 2 N-Linked glycans (2 sites) |
GlyGeni | Q3UQ28, 7 sites |
iPTMneti | Q3UQ28 |
PhosphoSitePlusi | Q3UQ28 |
Expressioni
Tissue specificityi
Highly expressed in the cardiovascular system. In the embryo, expressed in the corneal epithelial layer. In the adult eyes, expressed in the corneal and lens epithelium.2 Publications
Developmental stagei
Expressed in all embryonic tissues at 10 dpc. Detected at 7 dpc.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000020674, Expressed in metanephros and 256 other tissues |
ExpressionAtlasi | Q3UQ28, baseline and differential |
Genevisiblei | Q3UQ28, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 213608, 4 interactors |
STRINGi | 10090.ENSMUSP00000113703 |
Miscellaneous databases
RNActi | Q3UQ28, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 60 | LRRNTAdd BLAST | 37 | |
Repeati | 84 – 105 | LRR 1Add BLAST | 22 | |
Repeati | 108 – 129 | LRR 2Add BLAST | 22 | |
Repeati | 132 – 153 | LRR 3Add BLAST | 22 | |
Repeati | 156 – 177 | LRR 4Add BLAST | 22 | |
Domaini | 189 – 242 | LRRCTAdd BLAST | 54 | |
Domaini | 243 – 329 | Ig-like C2-type 1Add BLAST | 87 | |
Domaini | 339 – 425 | Ig-like C2-type 2Add BLAST | 87 | |
Domaini | 430 – 517 | Ig-like C2-type 3Add BLAST | 88 | |
Domaini | 518 – 607 | Ig-like C2-type 4Add BLAST | 90 | |
Domaini | 1409 – 1467 | VWFCPROSITE-ProRule annotationAdd BLAST | 59 |
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, Leucine-rich repeat, Repeat, SignalPhylogenomic databases
eggNOGi | KOG2408, Eukaryota |
GeneTreei | ENSGT00940000157666 |
HOGENOMi | CLU_006087_0_1_1 |
InParanoidi | Q3UQ28 |
OrthoDBi | 276568at2759 |
TreeFami | TF314316 |
Family and domain databases
CDDi | cd09826, peroxidasin_like, 1 hit |
Gene3Di | 1.10.640.10, 1 hit 2.60.40.10, 4 hits 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013106, Ig_V-set IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR034824, Peroxidasin_peroxidase IPR034828, Peroxidasin_vert IPR001007, VWF_dom |
PANTHERi | PTHR11475:SF75, PTHR11475:SF75, 3 hits |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF07679, I-set, 4 hits PF13855, LRR_8, 1 hit PF00093, VWC, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00409, IG, 4 hits SM00408, IGc2, 4 hits SM00406, IGv, 3 hits SM00369, LRR_TYP, 5 hits SM00082, LRRCT, 1 hit SM00214, VWC, 1 hit |
SUPFAMi | SSF48113, SSF48113, 1 hit SSF48726, SSF48726, 4 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 4 hits PS51450, LRR, 5 hits PS50292, PEROXIDASE_3, 1 hit PS01208, VWFC_1, 1 hit PS50184, VWFC_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q3UQ28-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAVRPTRRCL LALLLCFAWW AMAVVASKQG AGCPSRCLCF RTTVRCMHLL
60 70 80 90 100
LEAVPAVAPQ TSILDLRFNR IREIQPGAFR RLRSLNTLLL NNNQIKKIPN
110 120 130 140 150
GAFEDLENLK YLYLYKNEIQ SIDRQAFKGL ASLEQLYLHF NQIETLDPES
160 170 180 190 200
FQHLPKLERL FLHNNRITHL VPGTFSQLES MKRLRLDSNA LHCDCEILWL
210 220 230 240 250
ADLLKTYAQS GNAQAAATCE YPRRIQGRSV ATITPEELNC ERPRITSEPQ
260 270 280 290 300
DADVTSGNTV YFTCRAEGNP KPEIIWLRNN NELSMKTDSR LNLLDDGTLM
310 320 330 340 350
IQNTQEADEG VYQCMAKNVA GEAKTQEVTL RYLGSPARPT FVIQPQNTEV
360 370 380 390 400
LVGESVTLEC SATGHPLPQI TWTRGDRTPL PIDPRVNITP SGGLYIQNVA
410 420 430 440 450
QSDSGEYTCF ASNSVDSIHA TAFIIVQALP QFTVTPQSRV VIEGQTVDFQ
460 470 480 490 500
CAAKGHPQPV IAWTKGGSQL SVDRRHLVLS SGTLRISGVA LHDQGQYECQ
510 520 530 540 550
AVNIIGSQKV VAHLTVQPRV TPVFASIPSD MTVEVGTNVQ LPCSSQGEPE
560 570 580 590 600
PAITWNKDGV QVTESGKFHI SPEGFLTIND VGTADAGRYE CVARNTIGYA
610 620 630 640 650
SVSMVLSVNV PDVSRNGDPY VATSIVEAIA TVDRAINSTR THLFDSRPRS
660 670 680 690 700
PNDLLALFRY PRDPYTVGQA RAGEIFERTL QLIQEHVQHG LMVDLNGTSY
710 720 730 740 750
HYNDLVSPQY LSLIANLSGC TAHRRVNNCS DMCFHQKYRT HDGTCNNLQH
760 770 780 790 800
PMWGASLTAF ERLLKAVYEN GFNTPRGINS QRQYNGHVLP MPRLVSTTLI
810 820 830 840 850
GTEVITPDEQ FTHMLMQWGQ FLDHDLDSTV VALSQARFSD GQHCSSVCSN
860 870 880 890 900
DPPCFSVMIP PNDPRVRSGA RCMFFVRSSP VCGSGMTSLL MNSVYPREQI
910 920 930 940 950
NQLTSYIDAS NVYGSTDHEA RSIRDLASHR GLLRQGIVQR SGKPLLPFAT
960 970 980 990 1000
GPPTECMRDE NESPIPCFLA GDHRANEQLG LTSMHTLWFR EHNRIAAELL
1010 1020 1030 1040 1050
KLNPHWDGDT VYHETRKIVG AEIQHITYRH WLPKILGEVG MKMLGEYRGY
1060 1070 1080 1090 1100
DPSVNAGIFN AFATAAFRFG HTLINPLLYR LDENFEPIPQ GHVPLHKAFF
1110 1120 1130 1140 1150
SPFRIVNEGG IDPLLRGLFG VAGKMRIPSQ LLNTELTERL FSMAHTVALD
1160 1170 1180 1190 1200
LAAINIQRGR DHGIPPYHDY RVYCNLSAAY TFEDLKNEIK SPVIREKLQR
1210 1220 1230 1240 1250
LYGSTLNIDL FPALMVEDLV PGSRLGPTLM CLLSTQFRRL RDGDRLWYEN
1260 1270 1280 1290 1300
PGVFSPAQLT QLKQTSLARI LCDNSDNITR VQQDVFRVAE FPHGYSSCED
1310 1320 1330 1340 1350
IPRVDLRVWQ DCCEDCRTRG QFNAFSYHFR GRRSLEFSYE DDKPTKRARW
1360 1370 1380 1390 1400
RKALSVKHGK HLSNATSATH EHLEGPATND LKEFVLEMQK IITDLRKQIN
1410 1420 1430 1440 1450
SLESRLSTTE CVDDSGESHG GNTKWKKDPC TVCECKNGQI TCFVEACQPA
1460 1470
ACPQPVKVEG ACCPVCLKNT AEEKP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P6L9 | A0A1W2P6L9_MOUSE | Peroxidasin homolog | Pxdn | 1,295 | Annotation score: | ||
D3Z5M7 | D3Z5M7_MOUSE | Peroxidasin homolog | Pxdn | 307 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 549 | P → Q in BAE25216 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1298 | C → R in AAI12914 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK142872 mRNA Translation: BAE25216.1 AC159626 Genomic DNA No translation available. AC165078 Genomic DNA No translation available. BC112913 mRNA Translation: AAI12914.1 |
CCDSi | CCDS25856.1 |
RefSeqi | NP_852060.2, NM_181395.2 |
Genome annotation databases
Ensembli | ENSMUST00000122328; ENSMUSP00000113703; ENSMUSG00000020674 |
GeneIDi | 69675 |
KEGGi | mmu:69675 |
UCSCi | uc007ngl.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK142872 mRNA Translation: BAE25216.1 AC159626 Genomic DNA No translation available. AC165078 Genomic DNA No translation available. BC112913 mRNA Translation: AAI12914.1 |
CCDSi | CCDS25856.1 |
RefSeqi | NP_852060.2, NM_181395.2 |
3D structure databases
SMRi | Q3UQ28 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 213608, 4 interactors |
STRINGi | 10090.ENSMUSP00000113703 |
PTM databases
GlyConnecti | 2580, 2 N-Linked glycans (2 sites) |
GlyGeni | Q3UQ28, 7 sites |
iPTMneti | Q3UQ28 |
PhosphoSitePlusi | Q3UQ28 |
Proteomic databases
MaxQBi | Q3UQ28 |
PaxDbi | Q3UQ28 |
PRIDEi | Q3UQ28 |
ProteomicsDBi | 300361 |
Protocols and materials databases
Antibodypediai | 2426, 63 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000122328; ENSMUSP00000113703; ENSMUSG00000020674 |
GeneIDi | 69675 |
KEGGi | mmu:69675 |
UCSCi | uc007ngl.2, mouse |
Organism-specific databases
CTDi | 7837 |
MGIi | MGI:1916925, Pxdn |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG2408, Eukaryota |
GeneTreei | ENSGT00940000157666 |
HOGENOMi | CLU_006087_0_1_1 |
InParanoidi | Q3UQ28 |
OrthoDBi | 276568at2759 |
TreeFami | TF314316 |
Enzyme and pathway databases
Reactomei | R-MMU-2243919, Crosslinking of collagen fibrils |
Miscellaneous databases
BioGRID-ORCSi | 69675, 0 hits in 52 CRISPR screens |
ChiTaRSi | Pxdn, mouse |
PROi | PR:Q3UQ28 |
RNActi | Q3UQ28, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020674, Expressed in metanephros and 256 other tissues |
ExpressionAtlasi | Q3UQ28, baseline and differential |
Genevisiblei | Q3UQ28, MM |
Family and domain databases
CDDi | cd09826, peroxidasin_like, 1 hit |
Gene3Di | 1.10.640.10, 1 hit 2.60.40.10, 4 hits 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013106, Ig_V-set IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR034824, Peroxidasin_peroxidase IPR034828, Peroxidasin_vert IPR001007, VWF_dom |
PANTHERi | PTHR11475:SF75, PTHR11475:SF75, 3 hits |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF07679, I-set, 4 hits PF13855, LRR_8, 1 hit PF00093, VWC, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00409, IG, 4 hits SM00408, IGc2, 4 hits SM00406, IGv, 3 hits SM00369, LRR_TYP, 5 hits SM00082, LRRCT, 1 hit SM00214, VWC, 1 hit |
SUPFAMi | SSF48113, SSF48113, 1 hit SSF48726, SSF48726, 4 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 4 hits PS51450, LRR, 5 hits PS50292, PEROXIDASE_3, 1 hit PS01208, VWFC_1, 1 hit PS50184, VWFC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PXDN_MOUSE | |
Accessioni | Q3UQ28Primary (citable) accession number: Q3UQ28 Secondary accession number(s): A4FU83, E9QNQ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families