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Protein

Centrosomal protein of 63 kDa

Gene

Cep63

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for normal spindle assembly. Plays a key role in mother-centriole-dependent centriole duplication; the function seems also to involve CEP152, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication. Also recruits CDK1 to centrosomes. Plays a role in DNA damage response. Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes; this leads to the inactivation of spindle assembly and in delay mitotic progression.By similarity1 Publication

Miscellaneous

CEP63 and DEUP1 paralogs are both involved in centriole amplification: while CEP63 mediates mother-centriole-dependent centriole duplication, DEUP1 mediates de novo centriole amplification in multiciliated cells.1 Publication

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, DNA damage, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 63 kDa
Short name:
Cep63
Gene namesi
Name:Cep63
Synonyms:D9Mgc48e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2158560 Cep63

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003818041 – 700Centrosomal protein of 63 kDaAdd BLAST700

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei278PhosphoserineBy similarity1
Isoform 3 (identifier: Q3UPP8-3)
Modified residuei488PhosphoserineCombined sources1
Modified residuei492PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ3UPP8
PaxDbiQ3UPP8
PeptideAtlasiQ3UPP8
PRIDEiQ3UPP8

PTM databases

iPTMnetiQ3UPP8
PhosphoSitePlusiQ3UPP8

Expressioni

Gene expression databases

BgeeiENSMUSG00000032534 Expressed in 225 organ(s), highest expression level in testis
ExpressionAtlasiQ3UPP8 baseline and differential
GenevisibleiQ3UPP8 MM

Interactioni

Subunit structurei

Interacts with CEP152 and CDK1; these interactions recruit both ligands to centrosomes. Interacts with CDK2, CDK5RAP2, WDR62, CEP90, KIAA0753/moonraker and CCDC14. CEP63, CDK5RAP2, CEP152, WDR62 are proposed to form a stepwise assembled complex at the centrosome forming a ring near parental centrioles.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Cep152A2AUM92EBI-16081652,EBI-2554268

Protein-protein interaction databases

DIPiDIP-61756N
IntActiQ3UPP8, 1 interactor
STRINGi10090.ENSMUSP00000091306

Structurei

3D structure databases

ProteinModelPortaliQ3UPP8
SMRiQ3UPP8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili73 – 283Sequence analysisAdd BLAST211
Coiled coili343 – 533Sequence analysisAdd BLAST191

Sequence similaritiesi

Belongs to the CEP63 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFN5 Eukaryota
ENOG4111HAY LUCA
GeneTreeiENSGT00530000063797
HOGENOMiHOG000111548
InParanoidiQ3UPP8
PhylomeDBiQ3UPP8

Family and domain databases

InterProiView protein in InterPro
IPR029608 Cep63
IPR031470 Cep63/Deup1_N
PANTHERiPTHR18875:SF7 PTHR18875:SF7, 1 hit
PfamiView protein in Pfam
PF17045 CEP63, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q3UPP8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEALLEGIQN RGHSGGFLTS CEAELQELMK QIDIMVAHKK SEWEGQTHAL
60 70 80 90 100
ETCLDIRDRE LKALRSQLDM KHKEVGILHQ QIEEHEKTKQ EMAMEYKEEL
110 120 130 140 150
LKLQEELSRL KRSYEKLQKK QLREFRGNTK SFREDRSEIE RLTGKIEEFR
160 170 180 190 200
QKSLDWEKQR LIYQQQVSSL EAQRKALAEQ SEIIQAQLAN RKQKLESVEL
210 220 230 240 250
SSQSEIQHLN SKLERAKDTI CANELEIERL NIRVNDLMGT NMTILQDHRQ
260 270 280 290 300
KEEKLRESEK LLEALQEEQK ELKASLQSQE TFILEAKMQE KLQTTLKAVG
310 320 330 340 350
TQQSVERPLE DCQKERKYSS PGQGVLDNVL SQLDFSHSSE ELLQAEVTRL
360 370 380 390 400
EGSLESVSAT CKQLSQELME KYEELKRMEG HNNEYRTEIK KLKEQILQAD
410 420 430 440 450
QTYSSALEGM KMEISQLTRE LHQRDITIAS AKCSSSDMEK QLKAEMQKAE
460 470 480 490 500
EKAVEHKEIL SQLESLKLEN HRLSETVMKL ELGLHEAKEI SLADLQENYI
510 520 530 540 550
EALNKLVSEN QQLQKDLMST KSELEHATNM CKKKDGEIFN PAHSRAAGFK
560 570 580 590 600
NAELKPIHGQ HRHDGIKTEQ YKTGHHSPRG QTLDSIDPVA RGPSPLSSHI
610 620 630 640 650
SPGSSTVSLP SNFLFEAHSL PSVLDINDVN FSDSLSDCMN DQEEFVSSGS
660 670 680 690 700
LPTSPLGSIA TRFLEEEELR SHHILERLDA HIEELKRESE KTVRQFTALV
Length:700
Mass (Da):80,449
Last modified:September 1, 2009 - v2
Checksum:iDAF54534FF243E08
GO
Isoform 2 (identifier: Q3UPP8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.
     487-504: AKEISLADLQENYIEALN → VHTSTLSFCVFEAFERNC
     505-700: Missing.

Note: No experimental confirmation available.
Show »
Length:476
Mass (Da):55,732
Checksum:i1FB026E0A2CFFD71
GO
Isoform 3 (identifier: Q3UPP8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     487-648: Missing.

Show »
Length:538
Mass (Da):62,628
Checksum:i9D7C7DCC41CE2C54
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VPJ7F8VPJ7_MOUSE
Centrosomal protein of 63 kDa
Cep63
758Annotation score:
U5KVR9U5KVR9_MOUSE
Centrosomal protein 63
Cep63
596Annotation score:
A0A1L1SUS8A0A1L1SUS8_MOUSE
Centrosomal protein of 63 kDa
Cep63
139Annotation score:
A0A1L1SUB9A0A1L1SUB9_MOUSE
Centrosomal protein of 63 kDa
Cep63
181Annotation score:
E0CYR4E0CYR4_MOUSE
Centrosomal protein of 63 kDa
Cep63
88Annotation score:
E0CZ48E0CZ48_MOUSE
Centrosomal protein of 63 kDa
Cep63
71Annotation score:
E0CX88E0CX88_MOUSE
Centrosomal protein of 63 kDa
Cep63
78Annotation score:

Sequence cautioni

The sequence AAH48718 differs from that shown. Reason: Frameshift at positions 218 and 233.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0378431 – 28Missing in isoform 2. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_037844487 – 648Missing in isoform 3. 1 PublicationAdd BLAST162
Alternative sequenceiVSP_037845487 – 504AKEIS…IEALN → VHTSTLSFCVFEAFERNC in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_037846505 – 700Missing in isoform 2. 1 PublicationAdd BLAST196

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK143341 mRNA Translation: BAE25347.1
AL450317 Genomic DNA No translation available.
CT573150 Genomic DNA No translation available.
BC048718 mRNA Translation: AAH48718.1 Frameshift.
RefSeqiXP_006511808.1, XM_006511745.3 [Q3UPP8-1]
XP_017168899.1, XM_017313410.1 [Q3UPP8-1]
XP_017168903.1, XM_017313414.1 [Q3UPP8-3]
UniGeneiMm.38910

Genome annotation databases

EnsembliENSMUST00000162655; ENSMUSP00000125621; ENSMUSG00000032534 [Q3UPP8-3]
GeneIDi28135
UCSCiuc009rfs.1 mouse [Q3UPP8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK143341 mRNA Translation: BAE25347.1
AL450317 Genomic DNA No translation available.
CT573150 Genomic DNA No translation available.
BC048718 mRNA Translation: AAH48718.1 Frameshift.
RefSeqiXP_006511808.1, XM_006511745.3 [Q3UPP8-1]
XP_017168899.1, XM_017313410.1 [Q3UPP8-1]
XP_017168903.1, XM_017313414.1 [Q3UPP8-3]
UniGeneiMm.38910

3D structure databases

ProteinModelPortaliQ3UPP8
SMRiQ3UPP8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61756N
IntActiQ3UPP8, 1 interactor
STRINGi10090.ENSMUSP00000091306

PTM databases

iPTMnetiQ3UPP8
PhosphoSitePlusiQ3UPP8

Proteomic databases

MaxQBiQ3UPP8
PaxDbiQ3UPP8
PeptideAtlasiQ3UPP8
PRIDEiQ3UPP8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000162655; ENSMUSP00000125621; ENSMUSG00000032534 [Q3UPP8-3]
GeneIDi28135
UCSCiuc009rfs.1 mouse [Q3UPP8-1]

Organism-specific databases

CTDi80254
MGIiMGI:2158560 Cep63

Phylogenomic databases

eggNOGiENOG410IFN5 Eukaryota
ENOG4111HAY LUCA
GeneTreeiENSGT00530000063797
HOGENOMiHOG000111548
InParanoidiQ3UPP8
PhylomeDBiQ3UPP8

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Miscellaneous databases

ChiTaRSiCep63 mouse
PROiPR:Q3UPP8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032534 Expressed in 225 organ(s), highest expression level in testis
ExpressionAtlasiQ3UPP8 baseline and differential
GenevisibleiQ3UPP8 MM

Family and domain databases

InterProiView protein in InterPro
IPR029608 Cep63
IPR031470 Cep63/Deup1_N
PANTHERiPTHR18875:SF7 PTHR18875:SF7, 1 hit
PfamiView protein in Pfam
PF17045 CEP63, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCEP63_MOUSE
AccessioniPrimary (citable) accession number: Q3UPP8
Secondary accession number(s): Q80ZM0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: September 12, 2018
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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