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Protein

Protein transport protein Sec31A

Gene

Sec31a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (By similarity). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity).By similarity

GO - Molecular functioni

  • calcium-dependent protein binding Source: MGI
  • structural molecule activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-204005 COPII-mediated vesicle transport
R-MMU-2132295 MHC class II antigen presentation
R-MMU-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein Sec31A
Alternative name(s):
SEC31-like protein 1
SEC31-related protein A
Gene namesi
Name:Sec31a
Synonyms:Sec31l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1916412 Sec31a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002951481 – 1230Protein transport protein Sec31AAdd BLAST1230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei423Asymmetric dimethylarginineCombined sources1
Modified residuei526PhosphoserineCombined sources1
Modified residuei531PhosphoserineCombined sources1
Cross-linki646Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei798PhosphoserineBy similarity1
Modified residuei1171PhosphothreonineBy similarity1
Modified residuei1173PhosphoserineBy similarity1
Cross-linki1227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex, leading to regulate the size of COPII coats.By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ3UPL0
MaxQBiQ3UPL0
PaxDbiQ3UPL0
PeptideAtlasiQ3UPL0
PRIDEiQ3UPL0

PTM databases

iPTMnetiQ3UPL0
PhosphoSitePlusiQ3UPL0
SwissPalmiQ3UPL0

Expressioni

Gene expression databases

BgeeiENSMUSG00000035325 Expressed in 295 organ(s), highest expression level in colon
CleanExiMM_SEC31A
ExpressionAtlasiQ3UPL0 baseline and differential
GenevisibleiQ3UPL0 MM

Interactioni

Subunit structurei

COPII is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. SEC13 and SEC31 make a 2:2 tetramer that forms the edge element of the COPII outer coat. The tetramer self-assembles in multiple copies to form the complete polyhedral cage. Interacts (via WD 8) with SEC13 (By similarity). Interacts with PDCD6; interaction takes place in response to cytosolic calcium increase and leads to bridge together the BCR(KLHL12) complex and SEC31A, leading to monoubiquitination. Interacts with KLHL12 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213263, 3 interactors
IntActiQ3UPL0, 2 interactors
MINTiQ3UPL0
STRINGi10090.ENSMUSP00000092157

Structurei

3D structure databases

ProteinModelPortaliQ3UPL0
SMRiQ3UPL0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 47WD 1Add BLAST44
Repeati64 – 111WD 2Add BLAST48
Repeati120 – 160WD 3Add BLAST41
Repeati166 – 206WD 4Add BLAST41
Repeati209 – 254WD 5Add BLAST46
Repeati258 – 298WD 6Add BLAST41
Repeati301 – 342WD 7Add BLAST42
Repeati397 – 429WD 8; interaction with SEC13PROSITE-ProRule annotationAdd BLAST33

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni161 – 470Interaction with SEC13By similarityAdd BLAST310
Regioni799 – 1123Interaction with PDCD6By similarityAdd BLAST325

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi841 – 847ALG-2-binding site motif-2 (ABS-2),By similarity7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi832 – 1101Pro-richAdd BLAST270

Domaini

The ALG-2-binding site motif-2 (ABS-2) contains a PXPGF sequence that binds hydrophobic pocket 3 of PDCD6.By similarity

Sequence similaritiesi

Belongs to the WD repeat SEC31 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0307 Eukaryota
ENOG410XQ1D LUCA
GeneTreeiENSGT00390000003175
HOGENOMiHOG000230582
HOVERGENiHBG055626
InParanoidiQ3UPL0
KOiK14005
OMAiKDASFCY
OrthoDBiEOG091G01VB
PhylomeDBiQ3UPL0
TreeFamiTF313842

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q3UPL0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKLKEIDRTA MQAWSPAQNH PIYLATGTSA QQLDATFSTN ASLEIFELDL
60 70 80 90 100
SDPSLDMKSC ATFSSSHRYH KLIWGPHKMD SKGDVSGVLI AGGENGNIIL
110 120 130 140 150
YDPSKIIAGD KEVVIAQKDK HTGPVRALDV NIFQTNLVAS GANESEIYIW
160 170 180 190 200
DLNNFATPMT PGAKTQPPED ISCIAWNRQV QHILASASPS GRATVWDLRK
210 220 230 240 250
NEPIIKVSDH SNRMHCSGLA WHPDVATQMV LASEDDRLPV IQMWDLRFAS
260 270 280 290 300
SPLRVLENHA RGILAVAWSM ADPELLLSCG KDAKILCSNP NTGEVLYELP
310 320 330 340 350
TNTQWCFDIQ WCPRNPAVLS AASFDGRISV YSIMGGSIDG LRQKQVDKLS
360 370 380 390 400
SSFGNLDPFG TGQPLPPLQI PQQSAQHSIV LPLKKPPKWI RRPVGASFSF
410 420 430 440 450
GGKLVTFESV AVPLQQGAEQ QRRQPVFISQ VVTEKDFLNR SAQLQHAVQS
460 470 480 490 500
QGFIGYCQKK IEASQTEFEK NVWSFLKVNF EEDSRGKYLE LLGYRKEDLG
510 520 530 540 550
QKIALALNKV DGPDVALKDS DQVAQSDGEE SPAAEEQLLG ERIKEEKQEC
560 570 580 590 600
DFLPSAGGTF NISVSGDIDG LITRALLTGN FESAVDLCLH DNRMADAIIL
610 620 630 640 650
AIAGGQELLA QTQKKYFAKS QSKITRLITA VVMKNWREIV ESCDLKNWRE
660 670 680 690 700
ALAAVLTYAK PDEFSALCDL LGTRLEREGD SLLRTQACLC YICAGNVERL
710 720 730 740 750
VACWTKAQDG SSPLSLQDLI EKVVILRKAV QLTQALDTNT VGALLAEKMS
760 770 780 790 800
QYASLLAAQG SIAAALAFLP DNTNQPNIVQ LRDRLCKAQG KPVSGQESSQ
810 820 830 840 850
SPYERQPLSK GRPGPVAGHS QMPRVQTQQY YPHGENPPPP GFIMQGNVIP
860 870 880 890 900
NPAAPLPTAP GHMPSQLPPY PQPQPYQPAQ QYSFGTGGAA AYRPQQPVAP
910 920 930 940 950
PASNAYPNTP YISPVASYSG QPQMYTAQQA SSPTSSSAAS FPPPSSGASF
960 970 980 990 1000
QHGGPGAPPS SSAYALPPGT TGTPPAASEL PASQRTENQS FQDQASILEG
1010 1020 1030 1040 1050
PQNGWNDPPA LNRVPKKKKM PENFMPPVPI TSPIMNPSGD PQSQGLQQQP
1060 1070 1080 1090 1100
STPGPLSSHA SFPQQHLAGG QPFHGVQQPL AQTGMPPSFS KPNTEGAPGA
1110 1120 1130 1140 1150
PIGNTIQHVQ ALPTEKITKK PIPEEHLILK TTFEDLIQRC LSSATDPQTK
1160 1170 1180 1190 1200
RKLDDASKRL EFLYDKLREQ TLSPTIINGL HSIARSIETR NYSEGLSVHT
1210 1220 1230
HIVSTSNFSE TSAFMPVLKV VLSQASKLGV
Length:1,230
Mass (Da):133,569
Last modified:July 10, 2007 - v2
Checksum:iB6119EFDEF121A76
GO
Isoform 2 (identifier: Q3UPL0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     503-541: Missing.

Show »
Length:1,191
Mass (Da):129,564
Checksum:i06F5389DABBB32F9
GO
Isoform 3 (identifier: Q3UPL0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-923: Missing.

Note: No experimental confirmation available.
Show »
Length:307
Mass (Da):32,648
Checksum:iFD991C88AC4978E1
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R2A9S4R2A9_MOUSE
Protein transport protein Sec31A
Sec31a
934Annotation score:
S4R256S4R256_MOUSE
Protein transport protein Sec31A
Sec31a
364Annotation score:
S4R1T5S4R1T5_MOUSE
Protein transport protein Sec31A
Sec31a
264Annotation score:
S4R192S4R192_MOUSE
Protein transport protein Sec31A
Sec31a
248Annotation score:
S4R1Y8S4R1Y8_MOUSE
Protein transport protein Sec31A
Sec31a
296Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti820S → P in AAH54358 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0267521 – 923Missing in isoform 3. 1 PublicationAdd BLAST923
Alternative sequenceiVSP_026753503 – 541Missing in isoform 2. 1 PublicationAdd BLAST39

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC043942 mRNA Translation: AAH43942.1
BC054358 mRNA Translation: AAH54358.1
BC071249 mRNA Translation: AAH71249.1
AK134681 mRNA Translation: BAE22239.1
AK143455 mRNA Translation: BAE25385.1
CCDSiCCDS51573.1 [Q3UPL0-1]
RefSeqiNP_081245.1, NM_026969.1 [Q3UPL0-1]
XP_011247860.1, XM_011249558.2 [Q3UPL0-2]
UniGeneiMm.18634

Genome annotation databases

EnsembliENSMUST00000094578; ENSMUSP00000092157; ENSMUSG00000035325 [Q3UPL0-1]
ENSMUST00000182886; ENSMUSP00000138213; ENSMUSG00000035325 [Q3UPL0-2]
GeneIDi69162
KEGGimmu:69162
UCSCiuc008yhi.1 mouse [Q3UPL0-2]
uc008yhk.2 mouse [Q3UPL0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC043942 mRNA Translation: AAH43942.1
BC054358 mRNA Translation: AAH54358.1
BC071249 mRNA Translation: AAH71249.1
AK134681 mRNA Translation: BAE22239.1
AK143455 mRNA Translation: BAE25385.1
CCDSiCCDS51573.1 [Q3UPL0-1]
RefSeqiNP_081245.1, NM_026969.1 [Q3UPL0-1]
XP_011247860.1, XM_011249558.2 [Q3UPL0-2]
UniGeneiMm.18634

3D structure databases

ProteinModelPortaliQ3UPL0
SMRiQ3UPL0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213263, 3 interactors
IntActiQ3UPL0, 2 interactors
MINTiQ3UPL0
STRINGi10090.ENSMUSP00000092157

PTM databases

iPTMnetiQ3UPL0
PhosphoSitePlusiQ3UPL0
SwissPalmiQ3UPL0

Proteomic databases

EPDiQ3UPL0
MaxQBiQ3UPL0
PaxDbiQ3UPL0
PeptideAtlasiQ3UPL0
PRIDEiQ3UPL0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094578; ENSMUSP00000092157; ENSMUSG00000035325 [Q3UPL0-1]
ENSMUST00000182886; ENSMUSP00000138213; ENSMUSG00000035325 [Q3UPL0-2]
GeneIDi69162
KEGGimmu:69162
UCSCiuc008yhi.1 mouse [Q3UPL0-2]
uc008yhk.2 mouse [Q3UPL0-1]

Organism-specific databases

CTDi22872
MGIiMGI:1916412 Sec31a

Phylogenomic databases

eggNOGiKOG0307 Eukaryota
ENOG410XQ1D LUCA
GeneTreeiENSGT00390000003175
HOGENOMiHOG000230582
HOVERGENiHBG055626
InParanoidiQ3UPL0
KOiK14005
OMAiKDASFCY
OrthoDBiEOG091G01VB
PhylomeDBiQ3UPL0
TreeFamiTF313842

Enzyme and pathway databases

ReactomeiR-MMU-204005 COPII-mediated vesicle transport
R-MMU-2132295 MHC class II antigen presentation
R-MMU-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Miscellaneous databases

ChiTaRSiSec31a mouse
PROiPR:Q3UPL0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035325 Expressed in 295 organ(s), highest expression level in colon
CleanExiMM_SEC31A
ExpressionAtlasiQ3UPL0 baseline and differential
GenevisibleiQ3UPL0 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSC31A_MOUSE
AccessioniPrimary (citable) accession number: Q3UPL0
Secondary accession number(s): Q3UYH3
, Q6IQZ3, Q7TQJ7, Q811J4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: November 7, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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