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Protein

Zinc finger CCCH-type antiviral protein 1

Gene

Zc3hav1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Antiviral protein which inhibits the replication of viruses by recruiting the cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element (ZRE) present in the target viral mRNA, recruits cellular poly(A)-specific ribonuclease PARN to remove the poly(A) tail, and the 3'-5' exoribonuclease complex exosome to degrade the RNA body from the 3'-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72 (DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5'-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1 (HIV-1) and moloney and murine leukemia virus (MoMLV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), togaviridae: sindbis virus (SINV) and Ross river virus (RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation. Isoform 1 is a more potent viral inhibitor than isoform 2. Isoform 2 acts as a positive regulator of DDX58/RIG-I signaling resulting in activation of the downstream effector IRF3 leading to the expression of type I IFNs and IFN stimulated genes (ISGs).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri73 – 86C3H1-type 1PROSITE-ProRule annotationAdd BLAST14
Zinc fingeri87 – 113C3H1-type 1PROSITE-ProRule annotationAdd BLAST27
Zinc fingeri150 – 172C3H1-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri173 – 194C3H1-type 2PROSITE-ProRule annotationAdd BLAST22

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processAntiviral defense, Immunity, Innate immunity
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger CCCH-type antiviral protein 1
Alternative name(s):
ADP-ribosyltransferase diphtheria toxin-like 13
Short name:
ARTD13
Gene namesi
Name:Zc3hav1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1926031 Zc3hav1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003314611 – 946Zinc finger CCCH-type antiviral protein 1Add BLAST946

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei257PhosphoserineCombined sources1
Modified residuei262Phosphoserine; by GSK3-betaBy similarity1
Modified residuei265PhosphoserineCombined sources1
Modified residuei269PhosphoserineCombined sources1
Modified residuei273PhosphoserineCombined sources1
Modified residuei277PhosphothreonineCombined sources1
Modified residuei324PhosphoserineCombined sources1
Modified residuei350PhosphoserineCombined sources1
Modified residuei425PhosphoserineBy similarity1
Modified residuei508PhosphotyrosineCombined sources1
Modified residuei553PhosphoserineCombined sources1
Modified residuei583PhosphoserineBy similarity1
Modified residuei680PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-273 is essential for sequential phosphorylation of Ser-269, Ser-265, Ser-262 and Ser-257 by GSK3-beta. Phosphorylation by GSK3-beta enhances its antiviral activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3UPF5
MaxQBiQ3UPF5
PaxDbiQ3UPF5
PeptideAtlasiQ3UPF5
PRIDEiQ3UPF5

PTM databases

iPTMnetiQ3UPF5
PhosphoSitePlusiQ3UPF5

Expressioni

Gene expression databases

BgeeiENSMUSG00000029826 Expressed in 207 organ(s), highest expression level in mesenteric lymph node
ExpressionAtlasiQ3UPF5 baseline and differential
GenevisibleiQ3UPF5 MM

Interactioni

Subunit structurei

Homodimer or homooligomer. Homooligomerization is essential for its antiviral activity (By similarity). Interacts with EXOSC5 (By similarity). Interacts (via N-terminal domain) with DDX17 in an RNA-independent manner (By similarity). Interacts with EXOSC3, EXOSC7, DCP2 and DCP1A (By similarity). Interacts with PARN in an RNA-independent manner (By similarity). Interacts with XRN1 in an RNA-dependent manner (By similarity). Interacts (via N-terminal domain) with DHX30 (via N-terminus) in an RNA-independent manner (By similarity). Isoform 2 interacts (via zinc-fingers) with DDX58/RIG-I in an RNA-dependent manner (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ3UPF5, 3 interactors
MINTiQ3UPF5
STRINGi10090.ENSMUSP00000031850

Structurei

3D structure databases

ProteinModelPortaliQ3UPF5
SMRiQ3UPF5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini684 – 771WWEPROSITE-ProRule annotationAdd BLAST88
Domaini805 – 946PARP catalyticPROSITE-ProRule annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 254N-terminal domainAdd BLAST254
Regioni224 – 254Binding to EXOSC5By similarityAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 76Nuclear localization signalBy similarity8
Motifi283 – 290Nuclear export signalBy similarity8
Motifi412 – 413Nuclear localization signalSequence analysis2

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi572 – 576Poly-Met5

Domaini

The N-terminal domain is sufficient to bind to viral RNAs and promote their degradation. The second and fourth zinc fingers are involved in binding to specific viral RNAs (By similarity).By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri73 – 86C3H1-type 1PROSITE-ProRule annotationAdd BLAST14
Zinc fingeri87 – 113C3H1-type 1PROSITE-ProRule annotationAdd BLAST27
Zinc fingeri150 – 172C3H1-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri173 – 194C3H1-type 2PROSITE-ProRule annotationAdd BLAST22

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410KE5N Eukaryota
ENOG410YD9T LUCA
GeneTreeiENSGT00760000119084
HOGENOMiHOG000236279
HOVERGENiHBG050384
InParanoidiQ3UPF5
KOiK15259
PhylomeDBiQ3UPF5
TreeFamiTF338389

Family and domain databases

Gene3Di1.10.10.10, 1 hit
3.30.720.50, 1 hit
InterProiView protein in InterPro
IPR012317 Poly(ADP-ribose)pol_cat_dom
IPR036388 WH-like_DNA-bd_sf
IPR004170 WWE-dom
IPR037197 WWE_dom_sf
IPR000571 Znf_CCCH
PfamiView protein in Pfam
PF00644 PARP, 1 hit
PF02825 WWE, 1 hit
SUPFAMiSSF117839 SSF117839, 1 hit
PROSITEiView protein in PROSITE
PS51059 PARP_CATALYTIC, 1 hit
PS50918 WWE, 1 hit
PS50103 ZF_C3H1, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q3UPF5-1) [UniParc]FASTAAdd to basket
Also known as: ZAPL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTDPEVFCFI TKILCAHGGR MTLEELLGEI SLPEAQLYEL LKAAGPDRFV
60 70 80 90 100
LLETGDQAGI TRSVVATTRA RVCRRKYCQR PCDSLHLCKL NLLGRCHYAQ
110 120 130 140 150
SQRNLCKYSH DVLSEQNFQV LKNHELSGLN QEELAVLLVQ SDPFFMPEIC
160 170 180 190 200
KSYKGEGRKQ ICGQPQPCER LHICEHFTRG NCSYLNCLRS HNLMDRKVLA
210 220 230 240 250
IMREHGLSSD VVQNIQDICN NKHTRRNPPS MRAPHPHRRG GAHRDRSKSR
260 270 280 290 300
DRFHHNSLEV LSTVSPLGSG PPSPDVTGCK DPLEDVSADV TQKFKYLGTQ
310 320 330 340 350
DRAQLSSVSS KAAGVRGPSQ MRASQEFLED GDPDGLFSRN RSDSSTSRTS
360 370 380 390 400
AAGFPLVAAQ RNEAGAMKMG MPSGHHVEVK GKNEDIDRVP FLNSYIDGVT
410 420 430 440 450
MEEATVSGIL GKRATDNGLE EMILSSNHQK SVAKTQDPQT AGRITDSGQD
460 470 480 490 500
TAFLHSKYEE NPAWPGTSTH NGPNGFSQIM DETPNVSKSS PTGFGIKSAV
510 520 530 540 550
TGGKEAVYSG VQSLRSHVLA MPGETTTPVQ GSNRLPPSPL SSSTSHRVAA
560 570 580 590 600
SGSPGKSSTH ASVSPASEPS RMMMMMSDPA EYSLCYIVNP VSPRMDDHGL
610 620 630 640 650
KEICLDHLYR GCQQVNCNKN HFHLPYRWQL FILPTWMDFQ DMEYIERAYC
660 670 680 690 700
DPQIEIIVIE KHRINFKKMT CDSYPIRRLS TPSFVEKTLN SVFTTKWLWY
710 720 730 740 750
WRNELNEYTQ YGHESPSHTS SEINSAYLES FFHSCPRGVL QFHAGSQNYE
760 770 780 790 800
LSFQGMIQTN IASKTQRHVV RRPVFVSSKD VEQKRRGPDH QPVMPQADAL
810 820 830 840 850
TLFSSPQRNA STVSSNEYEF IELNNQDEEY AKISEQFKAS MKQFKIVTIK
860 870 880 890 900
RIWNQKLWDT FERKKQKMKN KTEMFLFHAV GRIHMDYICK NNFEWILHGN
910 920 930 940
REIRYGKGLC WRRENCDSSH AHGFLEMPLA SLGRTASLDS SGLQRK
Length:946
Mass (Da):106,688
Last modified:October 11, 2005 - v1
Checksum:iBE2EFE5FBC6062EA
GO
Isoform 2 (identifier: Q3UPF5-2) [UniParc]FASTAAdd to basket
Also known as: ZAPS

The sequence of this isoform differs from the canonical sequence as follows:
     789-789: D → E
     790-946: Missing.

Show »
Length:789
Mass (Da):88,231
Checksum:i98C5633AE78BD4A5
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z5I1D3Z5I1_MOUSE
Zinc finger CCCH-type antiviral pro...
Zc3hav1
996Annotation score:
G3X9X5G3X9X5_MOUSE
Zinc finger CCCH type, antiviral 1,...
Zc3hav1 mCG_121839
673Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_033213789D → E in isoform 2. 1 Publication1
Alternative sequenceiVSP_033214790 – 946Missing in isoform 2. 1 PublicationAdd BLAST157

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004770 mRNA Translation: BAB23549.1
AK080334 mRNA Translation: BAC37881.1
AK143568 mRNA Translation: BAE25440.1
AK169402 mRNA Translation: BAE41148.1
AK172379 mRNA Translation: BAE42974.1
AK020263 mRNA Translation: BAB32047.2
CCDSiCCDS20012.1 [Q3UPF5-2]
CCDS80522.1 [Q3UPF5-1]
RefSeqiNP_082697.1, NM_028421.1 [Q3UPF5-1]
NP_083140.1, NM_028864.2 [Q3UPF5-2]
UniGeneiMm.25243
Mm.486484

Genome annotation databases

EnsembliENSMUST00000031850; ENSMUSP00000031850; ENSMUSG00000029826 [Q3UPF5-2]
ENSMUST00000143702; ENSMUSP00000144312; ENSMUSG00000029826 [Q3UPF5-1]
GeneIDi78781
KEGGimmu:78781
UCSCiuc009bjz.2 mouse [Q3UPF5-1]
uc009bka.1 mouse [Q3UPF5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004770 mRNA Translation: BAB23549.1
AK080334 mRNA Translation: BAC37881.1
AK143568 mRNA Translation: BAE25440.1
AK169402 mRNA Translation: BAE41148.1
AK172379 mRNA Translation: BAE42974.1
AK020263 mRNA Translation: BAB32047.2
CCDSiCCDS20012.1 [Q3UPF5-2]
CCDS80522.1 [Q3UPF5-1]
RefSeqiNP_082697.1, NM_028421.1 [Q3UPF5-1]
NP_083140.1, NM_028864.2 [Q3UPF5-2]
UniGeneiMm.25243
Mm.486484

3D structure databases

ProteinModelPortaliQ3UPF5
SMRiQ3UPF5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3UPF5, 3 interactors
MINTiQ3UPF5
STRINGi10090.ENSMUSP00000031850

PTM databases

iPTMnetiQ3UPF5
PhosphoSitePlusiQ3UPF5

Proteomic databases

EPDiQ3UPF5
MaxQBiQ3UPF5
PaxDbiQ3UPF5
PeptideAtlasiQ3UPF5
PRIDEiQ3UPF5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031850; ENSMUSP00000031850; ENSMUSG00000029826 [Q3UPF5-2]
ENSMUST00000143702; ENSMUSP00000144312; ENSMUSG00000029826 [Q3UPF5-1]
GeneIDi78781
KEGGimmu:78781
UCSCiuc009bjz.2 mouse [Q3UPF5-1]
uc009bka.1 mouse [Q3UPF5-2]

Organism-specific databases

CTDi56829
MGIiMGI:1926031 Zc3hav1

Phylogenomic databases

eggNOGiENOG410KE5N Eukaryota
ENOG410YD9T LUCA
GeneTreeiENSGT00760000119084
HOGENOMiHOG000236279
HOVERGENiHBG050384
InParanoidiQ3UPF5
KOiK15259
PhylomeDBiQ3UPF5
TreeFamiTF338389

Miscellaneous databases

PROiPR:Q3UPF5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029826 Expressed in 207 organ(s), highest expression level in mesenteric lymph node
ExpressionAtlasiQ3UPF5 baseline and differential
GenevisibleiQ3UPF5 MM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
3.30.720.50, 1 hit
InterProiView protein in InterPro
IPR012317 Poly(ADP-ribose)pol_cat_dom
IPR036388 WH-like_DNA-bd_sf
IPR004170 WWE-dom
IPR037197 WWE_dom_sf
IPR000571 Znf_CCCH
PfamiView protein in Pfam
PF00644 PARP, 1 hit
PF02825 WWE, 1 hit
SUPFAMiSSF117839 SSF117839, 1 hit
PROSITEiView protein in PROSITE
PS51059 PARP_CATALYTIC, 1 hit
PS50918 WWE, 1 hit
PS50103 ZF_C3H1, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiZCCHV_MOUSE
AccessioniPrimary (citable) accession number: Q3UPF5
Secondary accession number(s): Q9CTU4, Q9DBS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 11, 2005
Last modified: November 7, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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