Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

EF-hand calcium-binding domain-containing protein 4B

Gene

Cracr2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ca2+-binding protein that plays a key role in store-operated Ca2+ entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca2+ concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca2+ resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi91 – 102PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Calcium transport, Immunity, Ion transport, Transport
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
EF-hand calcium-binding domain-containing protein 4B
Alternative name(s):
Calcium release-activated channel regulator 2A
Gene namesi
Name:Cracr2a
Synonyms:Efcab4b, Gm1073
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2685919 Cracr2a

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002830471 – 310EF-hand calcium-binding domain-containing protein 4BAdd BLAST310

Proteomic databases

MaxQBiQ3UP38
PaxDbiQ3UP38
PRIDEiQ3UP38

PTM databases

iPTMnetiQ3UP38
PhosphoSitePlusiQ3UP38

Expressioni

Gene expression databases

BgeeiENSMUSG00000061414 Expressed in 21 organ(s), highest expression level in brown adipose tissue
CleanExiMM_EFCAB4B
ExpressionAtlasiQ3UP38 baseline and differential

Interactioni

Subunit structurei

Interacts with ORAI1 and STIM1; the interaction is direct and takes place in absence of Ca2+. Forms a complex with ORAI1 and STIM1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations. Interacts with ORAI2 and ORAI3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071494

Structurei

3D structure databases

ProteinModelPortaliQ3UP38
SMRiQ3UP38
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 76EF-hand 1PROSITE-ProRule annotationAdd BLAST29
Domaini78 – 113EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili192 – 282Sequence analysisAdd BLAST91

Sequence similaritiesi

Belongs to the EFCAB4 family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IFG3 Eukaryota
ENOG410YP84 LUCA
GeneTreeiENSGT00440000033504
HOGENOMiHOG000112335
HOVERGENiHBG107143
InParanoidiQ3UP38
KOiK17199
OrthoDBiEOG091G0CBJ
PhylomeDBiQ3UP38
TreeFamiTF329556

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q3UP38-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATPSGREDS SSQTPGHGKQ GSGACVEQLD HPEKLEVEMP DQSAMWKKAQ
60 70 80 90 100
EFFQTCDSEG KGFIARTDMQ RLHQELPLSL EELEDVFDAL DADGNGFLTP
110 120 130 140 150
EEFTTGFSHF FFSQNIQGEE EADQQVAQLQ EEKVYQSRGE EDVGDMDHDE
160 170 180 190 200
EAQFQMLMDR LGAQKVLEDE SDVRQLWLQL RKDEPHLLSN FEDLLTTIFA
210 220 230 240 250
QLQEAHEQKN ELECALRKKI AAYDEEIQHL YEEMEQQIKS EREQFLLKDT
260 270 280 290 300
ERFQARSREL EKKLSAKEQE LERLNQKQRK VGYCGDIVGP QLFQLSLPLP
310
HALHHSSMDF
Length:310
Mass (Da):35,840
Last modified:October 11, 2005 - v1
Checksum:iCCEC6E9ED3F8ABF7
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1D5RLZ8A0A1D5RLZ8_MOUSE
EF-hand calcium-binding domain-cont...
Cracr2a
726Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti272E → G in AAI50870 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK143832 mRNA Translation: BAE25559.1
BC150869 mRNA Translation: AAI50870.1
CCDSiCCDS20566.1
RefSeqiNP_001028636.1, NM_001033464.3
UniGeneiMm.296093

Genome annotation databases

EnsembliENSMUST00000071563; ENSMUSP00000071494; ENSMUSG00000061414
GeneIDi381812
KEGGimmu:381812
UCSCiuc009dwa.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK143832 mRNA Translation: BAE25559.1
BC150869 mRNA Translation: AAI50870.1
CCDSiCCDS20566.1
RefSeqiNP_001028636.1, NM_001033464.3
UniGeneiMm.296093

3D structure databases

ProteinModelPortaliQ3UP38
SMRiQ3UP38
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071494

PTM databases

iPTMnetiQ3UP38
PhosphoSitePlusiQ3UP38

Proteomic databases

MaxQBiQ3UP38
PaxDbiQ3UP38
PRIDEiQ3UP38

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071563; ENSMUSP00000071494; ENSMUSG00000061414
GeneIDi381812
KEGGimmu:381812
UCSCiuc009dwa.1 mouse

Organism-specific databases

CTDi84766
MGIiMGI:2685919 Cracr2a

Phylogenomic databases

eggNOGiENOG410IFG3 Eukaryota
ENOG410YP84 LUCA
GeneTreeiENSGT00440000033504
HOGENOMiHOG000112335
HOVERGENiHBG107143
InParanoidiQ3UP38
KOiK17199
OrthoDBiEOG091G0CBJ
PhylomeDBiQ3UP38
TreeFamiTF329556

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiCracr2b mouse
PROiPR:Q3UP38
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000061414 Expressed in 21 organ(s), highest expression level in brown adipose tissue
CleanExiMM_EFCAB4B
ExpressionAtlasiQ3UP38 baseline and differential

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEFC4B_MOUSE
AccessioniPrimary (citable) accession number: Q3UP38
Secondary accession number(s): B9EKF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 11, 2005
Last modified: September 12, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again