UniProtKB - Q3UMR5 (MCU_MOUSE)
Calcium uniporter protein, mitochondrial
Mcu
Functioni
Activity regulationi
GO - Molecular functioni
- calcium channel activity Source: UniProtKB
- identical protein binding Source: IntAct
- uniporter activity Source: UniProtKB
GO - Biological processi
- actin filament reorganization Source: MGI
- calcium import into the mitochondrion Source: UniProtKB
- calcium-mediated signaling Source: UniProtKB
- glucose homeostasis Source: UniProtKB
- mitochondrial calcium ion homeostasis Source: MGI
- mitochondrial calcium ion transmembrane transport Source: UniProtKB
- positive regulation of insulin secretion Source: UniProtKB
- positive regulation of mitochondrial calcium ion concentration Source: UniProtKB
- positive regulation of mitochondrial fission Source: MGI
- positive regulation of neutrophil chemotaxis Source: MGI
- protein complex oligomerization Source: UniProtKB
Keywordsi
Molecular function | Calcium channel, Ion channel |
Biological process | Calcium transport, Ion transport, Transport |
Ligand | Calcium |
Enzyme and pathway databases
Reactomei | R-MMU-8949215, Mitochondrial calcium ion transport R-MMU-8949664, Processing of SMDT1 |
Protein family/group databases
TCDBi | 1.A.77.1.1, the mg(2+)/ca(2+) uniporter (mcu) family |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium uniporter protein, mitochondrialCurated |
Gene namesi | Name:McuImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3026965, Mcu |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication1 Publication; Multi-pass membrane protein 1 Publication
Mitochondrion
- integral component of mitochondrial inner membrane Source: UniProtKB
- mitochondrial inner membrane Source: UniProtKB
- mitochondrion Source: MGI
- uniplex complex Source: MGI
Other locations
- calcium channel complex Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 50 – 232 | Mitochondrial matrixBy similarityAdd BLAST | 183 | |
Transmembranei | 233 – 256 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 257 – 264 | Mitochondrial intermembraneBy similarity | 8 | |
Transmembranei | 265 – 282 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 283 – 350 | Mitochondrial matrixBy similarityAdd BLAST | 68 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 251 | R → W: Strongly reduces calcium channel activity; when associated with V-256. 1 Publication | 1 | |
Mutagenesisi | 256 | E → V: Strongly reduces calcium channel activity; when associated with W-251. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 49 | MitochondrionSequence analysisAdd BLAST | 49 | |
ChainiPRO_0000282977 | 50 – 350 | Calcium uniporter protein, mitochondrialAdd BLAST | 301 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 56 | Phosphoserine; by CaMK2By similarity | 1 | |
Modified residuei | 91 | Phosphoserine; by CaMK2By similarity | 1 | |
Modified residuei | 331 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q3UMR5 |
jPOSTi | Q3UMR5 |
MaxQBi | Q3UMR5 |
PaxDbi | Q3UMR5 |
PeptideAtlasi | Q3UMR5 |
PRIDEi | Q3UMR5 |
PTM databases
iPTMneti | Q3UMR5 |
PhosphoSitePlusi | Q3UMR5 |
SwissPalmi | Q3UMR5 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000009647, Expressed in intestine and 261 other tissues |
Genevisiblei | Q3UMR5, MM |
Interactioni
Subunit structurei
Component of the uniplex complex, composed of MCU, MCUB, MICU1, MICU2 and EMRE/SMDT1 (By similarity). Heterooligomer with CCDC109B/MCUB; this inhibits channel activity (PubMed:23900286). Homooligomer. Homotetramer (PubMed:23900286).
Interacts with MICU1; MICU1 acts as an essential regulator for MCU.
Interacts with MCUR1. Interactions with MICU1 and MCUR1 are mutually exclusive.
Interacts with MICU2 (By similarity).
Interacts with SLC25A23 (By similarity).
By similarity2 PublicationsBinary interactionsi
Hide detailsQ3UMR5
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-776370,EBI-776370 |
Mcub [Q810S1] | 3 | EBI-776370,EBI-8847756 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 229684, 1 interactor |
IntActi | Q3UMR5, 4 interactors |
MINTi | Q3UMR5 |
STRINGi | 10090.ENSMUSP00000020312 |
Miscellaneous databases
RNActi | Q3UMR5, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 74 – 164 | N-terminal MCU domainBy similarityAdd BLAST | 91 | |
Regioni | 215 – 233 | Outer juxtamembrane helix (OJMH)By similarityAdd BLAST | 19 | |
Regioni | 282 – 291 | Inner juxtamembrane helix (IJMH)By similarity | 10 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 191 – 220 | Sequence analysisAdd BLAST | 30 | |
Coiled coili | 310 – 338 | Sequence analysisAdd BLAST | 29 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 260 – 263 | DXXEBy similarity | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2966, Eukaryota |
GeneTreei | ENSGT00940000157528 |
HOGENOMi | CLU_056554_0_0_1 |
InParanoidi | Q3UMR5 |
OMAi | CIEEHQL |
OrthoDBi | 1076768at2759 |
PhylomeDBi | Q3UMR5 |
TreeFami | TF314435 |
Family and domain databases
InterProi | View protein in InterPro IPR006769, MCU_C IPR039055, MCU_fam |
PANTHERi | PTHR13462, PTHR13462, 1 hit |
Pfami | View protein in Pfam PF04678, MCU, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAAGRSLL LLLCSRGGGG GAGGCGALTA GCFPGLGVSR HRPHQQHRTA
60 70 80 90 100
HQRPASWQSV GAAYCSTVVP SDDVTVVYQN GLPVISVRLP SRRERCQFTL
110 120 130 140 150
KPISDSVGVF LRQLQEEDRG IDRVAIYSPD GVRVAASTGI DLLLLDDFKL
160 170 180 190 200
VINDLTYHVR PPKRDLLSHE DAATLNDVKT LVQQLYTTLC IEQHQLNKER
210 220 230 240 250
ELVERLEDLK QQLAPLEKVR IEISRKAEKR TTLVLWGGLA YMATQFGILA
260 270 280 290 300
RLTWWEYSWD IMEPVTYFIT YGSAMAMYAY FVMTRQEYVY PEARDRQYLL
310 320 330 340 350
FFHKGAKKSR FDLEKYNQLK DAIAQAEMDL KRLRDPLQVH LPLRQIGEKE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE0CYL3 | E0CYL3_MOUSE | Calcium uniporter protein, mitochon... | Mcu Ccdc109a | 53 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024264 | 1 – 149 | Missing in isoform 2. 2 PublicationsAdd BLAST | 149 | |
Alternative sequenceiVSP_024265 | 150 – 164 | LVIND…RPPKR → MILRPKQLFSAFTKQ in isoform 2. 2 PublicationsAdd BLAST | 15 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK144727 mRNA Translation: BAE26033.1 AK161322 mRNA Translation: BAE36322.1 AC155940 Genomic DNA No translation available. AC022699 Genomic DNA No translation available. BC139254 mRNA Translation: AAI39255.1 BC139257 mRNA Translation: AAI39258.1 |
CCDSi | CCDS23866.2 [Q3UMR5-1] |
RefSeqi | NP_001028431.2, NM_001033259.4 [Q3UMR5-1] |
Genome annotation databases
Ensembli | ENSMUST00000009791; ENSMUSP00000009791; ENSMUSG00000009647 [Q3UMR5-2] ENSMUST00000020312; ENSMUSP00000020312; ENSMUSG00000009647 [Q3UMR5-1] |
GeneIDi | 215999 |
KEGGi | mmu:215999 |
UCSCi | uc007fds.3, mouse [Q3UMR5-2] uc007fdt.3, mouse [Q3UMR5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK144727 mRNA Translation: BAE26033.1 AK161322 mRNA Translation: BAE36322.1 AC155940 Genomic DNA No translation available. AC022699 Genomic DNA No translation available. BC139254 mRNA Translation: AAI39255.1 BC139257 mRNA Translation: AAI39258.1 |
CCDSi | CCDS23866.2 [Q3UMR5-1] |
RefSeqi | NP_001028431.2, NM_001033259.4 [Q3UMR5-1] |
3D structure databases
SMRi | Q3UMR5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229684, 1 interactor |
IntActi | Q3UMR5, 4 interactors |
MINTi | Q3UMR5 |
STRINGi | 10090.ENSMUSP00000020312 |
Protein family/group databases
TCDBi | 1.A.77.1.1, the mg(2+)/ca(2+) uniporter (mcu) family |
PTM databases
iPTMneti | Q3UMR5 |
PhosphoSitePlusi | Q3UMR5 |
SwissPalmi | Q3UMR5 |
Proteomic databases
EPDi | Q3UMR5 |
jPOSTi | Q3UMR5 |
MaxQBi | Q3UMR5 |
PaxDbi | Q3UMR5 |
PeptideAtlasi | Q3UMR5 |
PRIDEi | Q3UMR5 |
Protocols and materials databases
Antibodypediai | 3091, 63 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000009791; ENSMUSP00000009791; ENSMUSG00000009647 [Q3UMR5-2] ENSMUST00000020312; ENSMUSP00000020312; ENSMUSG00000009647 [Q3UMR5-1] |
GeneIDi | 215999 |
KEGGi | mmu:215999 |
UCSCi | uc007fds.3, mouse [Q3UMR5-2] uc007fdt.3, mouse [Q3UMR5-1] |
Organism-specific databases
CTDi | 90550 |
MGIi | MGI:3026965, Mcu |
Phylogenomic databases
eggNOGi | KOG2966, Eukaryota |
GeneTreei | ENSGT00940000157528 |
HOGENOMi | CLU_056554_0_0_1 |
InParanoidi | Q3UMR5 |
OMAi | CIEEHQL |
OrthoDBi | 1076768at2759 |
PhylomeDBi | Q3UMR5 |
TreeFami | TF314435 |
Enzyme and pathway databases
Reactomei | R-MMU-8949215, Mitochondrial calcium ion transport R-MMU-8949664, Processing of SMDT1 |
Miscellaneous databases
BioGRID-ORCSi | 215999, 2 hits in 17 CRISPR screens |
ChiTaRSi | Mcu, mouse |
PROi | PR:Q3UMR5 |
RNActi | Q3UMR5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000009647, Expressed in intestine and 261 other tissues |
Genevisiblei | Q3UMR5, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006769, MCU_C IPR039055, MCU_fam |
PANTHERi | PTHR13462, PTHR13462, 1 hit |
Pfami | View protein in Pfam PF04678, MCU, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCU_MOUSE | |
Accessioni | Q3UMR5Primary (citable) accession number: Q3UMR5 Secondary accession number(s): B2RTD1, Q3TTK3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | April 3, 2007 | |
Last modified: | December 2, 2020 | |
This is version 114 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families