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Protein

Ankyrin repeat domain-containing protein 27

Gene

Ankrd27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (By similarity). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (By similarity). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (PubMed:19403694, PubMed:21187289). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (PubMed:19745841, PubMed:22171327). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1. Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (By similarity). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (By similarity). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (PubMed:26620560).By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation, Guanine-nucleotide releasing factor
Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat domain-containing protein 27
Alternative name(s):
VPS9 domain-containing protein
VPS9-ankyrin-repeat protein
Gene namesi
Name:Ankrd27
Synonyms:Varp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2444103 Ankrd27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi310D → A: Inhibits dendrite formation. 1 Publication1
Mutagenesisi350Y → A: Inhibits dendrite formation. 1 Publication1
Mutagenesisi509Q → A: Disrupts interaction with RAB32 and RAB38; inhibits peripheral distribution of TYRP1 in melanocytes; no effect on dendrite formation in melanocytes. 2 Publications1
Mutagenesisi550Y → A: Disrupts interaction with RAB32 and RAB38; inhibits peripheral distribution of TYRP1 in melanocytes; no effect on dendrite formation in melanocytes. 2 Publications1
Mutagenesisi575W → A: Impairs interaction with RAB32 and RAB38. 1 Publication1
Mutagenesisi577Y → A: Impairs interaction with RAB32 and RAB38. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002745571 – 1048Ankyrin repeat domain-containing protein 27Add BLAST1048

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei961PhosphoserineCombined sources1
Modified residuei969PhosphoserineBy similarity1
Modified residuei1022PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UMR0
PaxDbiQ3UMR0
PRIDEiQ3UMR0

PTM databases

iPTMnetiQ3UMR0
PhosphoSitePlusiQ3UMR0

Expressioni

Gene expression databases

BgeeiENSMUSG00000034867 Expressed in 237 organ(s), highest expression level in embryo
CleanExiMM_ANKRD27
ExpressionAtlasiQ3UMR0 baseline and differential
GenevisibleiQ3UMR0 MM

Interactioni

Subunit structurei

Interacts with RAB21 (GDP-bound form), VPS29, KIF5A, KIF5C, GOLGA4 (By similarity). Interacts with RAB32 (GTP-bound form), RAB38 (GTP-bound form), VAMP7 (PubMed:19403694, PubMed:21187289). Interacts with low affinity with RAB5 (By similarity). ANKRD27:RAB32 heterodimers can homodimerize to form tetramers (By similarity). Can interact with RAB38 or RAB32, VPS29 and VAMP7 simultaneously (By similarity). A decreased interaction with RAB32 seen in the presence of SGSM2 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rab38Q8QZZ85EBI-1993429,EBI-1993463

GO - Molecular functioni

Protein-protein interaction databases

BioGridi232848, 1 interactor
IntActiQ3UMR0, 2 interactors
STRINGi10090.ENSMUSP00000041751

Structurei

3D structure databases

ProteinModelPortaliQ3UMR0
SMRiQ3UMR0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini233 – 371VPS9PROSITE-ProRule annotationAdd BLAST139
Repeati396 – 426ANK 1Add BLAST31
Repeati462 – 491ANK 2Add BLAST30
Repeati495 – 524ANK 3Add BLAST30
Repeati528 – 557ANK 4Add BLAST30
Repeati564 – 593ANK 5Add BLAST30
Repeati597 – 627ANK 6Add BLAST31
Repeati668 – 698ANK 7Add BLAST31
Repeati742 – 771ANK 8Add BLAST30
Repeati775 – 804ANK 9Add BLAST30
Repeati808 – 837ANK 10Add BLAST30
Repeati841 – 870ANK 11Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 372Sufficient for GEF activity towards RAB21By similarityAdd BLAST372
Regioni396 – 460Sufficient for interaction with VPS29By similarityAdd BLAST65
Regioni451 – 729Interaction with RAB321 PublicationAdd BLAST279
Regioni451 – 600Interaction with RAB381 PublicationAdd BLAST150
Regioni658 – 707Required for interaction with VAMP7By similarity1 PublicationAdd BLAST50
Regioni692 – 745Sufficient for interaction with VPS29By similarityAdd BLAST54

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IQ81 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00930000150856
HOGENOMiHOG000033958
HOVERGENiHBG080845
InParanoidiQ3UMR0
KOiK20175
OrthoDBiEOG091G07HY
PhylomeDBiQ3UMR0
TreeFamiTF351261

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.20.1050.80, 1 hit
1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR003123 VPS9
IPR037191 VPS9_dom_sf
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF02204 VPS9, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 8 hits
SM00167 VPS9, 1 hit
SUPFAMiSSF109993 SSF109993, 1 hit
SSF48403 SSF48403, 2 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 8 hits
PS51205 VPS9, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q3UMR0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALYDEDLLK NPFYLALQKW RPDLCSKVAQ IHGIVLVPCR GSLPGSVQAS
60 70 80 90 100
CQFESYVLVP TEGHFQTLDG KAVVIEGNRI KLGAGFACLL SVPILFEETF
110 120 130 140 150
YNEKEESFSI LCIAHPLERR ETSEEPSAPA DPFSLKTIED VREFLGRHSE
160 170 180 190 200
KFDKNIASFH RTFRECERKS LRHHIDSVNA LYTKCLQQLL RDSHLKVLAK
210 220 230 240 250
QEAQMNLMKQ AVEMYVHHDI YDLIFKYVGT MEASEDAAFN KITRSLQDLQ
260 270 280 290 300
QKDIGVKPEF SFNIPRAKRE LGQLNKCTSP QQKLLCLRKV VQLMTQSPSQ
310 320 330 340 350
RVNLETMCAD DLLSVLLYLL VKTEIPNWMA NLSYIKNFRF SSSAKDELGY
360 370 380 390 400
CLTSVEAAIE YIRQGSLSTK TPDAEGFGDR LFLKQRMNLL SQMTSTPIDC
410 420 430 440 450
LFKHIASGNQ KEVERLLSQD DQDKDAMQKM CHPLCSCEDC EKLISGRLND
460 470 480 490 500
PSVVTPFSRD DRGQTPLHVA ALCGQASLID FLVSKGAVVN ATDYHGSTPL
510 520 530 540 550
HLACQKGFQS VTLLLLHYKA STEVQDNNGN TPLHLACTYG QEDCVKALVY
560 570 580 590 600
YDVQACRLDI GNEKGDTALH IAARWGYEGI IETLLQNGAP TAVQNRLKET
610 620 630 640 650
PLKCALNSKI LSIMEAHHLS SDRRPRPSEV PAQSPTRSVD SISQGSSTSS
660 670 680 690 700
FSSISVSFRQ EEVKKDYREV EKLLRAVADG DLEMVRYLLE WTEDDLDDVE
710 720 730 740 750
DAISTVDLEF CHPLCQCPKC APAQKLARIS ANGLSVNVTN QDGFSPLHMA
760 770 780 790 800
ALHGRTDLVP LLLKHGAYSG ARNTSQAVPL HLACQQGHFQ VAKCLLDSNA
810 820 830 840 850
KPNKKDLSGN TPLICACSAG HHEVAALLLQ HGASINACNN KGNTALHEAV
860 870 880 890 900
MGRHTLVVEL LLFYGASVDI LNKRQYTAAD CAEQDSKIME LLQVVPGCVA
910 920 930 940 950
SLDSVEEADH EGYVTVEIRR KWNPKMYNLP EEPLRRQFCL INPGGRFQER
960 970 980 990 1000
TSRETMGRDR SVPDLAGRSL QEPEKQRVTG KQSDLSDLSR YQTSEEGNKG
1010 1020 1030 1040
LPERPVSRQA APGHRPMVRR HTVNDAAILQ VPEVTVHLTT HEASVPQS
Length:1,048
Mass (Da):116,809
Last modified:February 6, 2007 - v2
Checksum:i63636820D0CC4D2C
GO
Isoform 2 (identifier: Q3UMR0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     670-724: Missing.

Note: No experimental confirmation available.
Show »
Length:993
Mass (Da):110,604
Checksum:i855BDE37439764D2
GO
Isoform 3 (identifier: Q3UMR0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     630-670: Missing.

Note: No experimental confirmation available.
Show »
Length:1,007
Mass (Da):112,333
Checksum:iD14EAB10424F63F5
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WS06A0A087WS06_MOUSE
Ankyrin repeat domain-containing pr...
Ankrd27
231Annotation score:
A0A0R4J2C4A0A0R4J2C4_MOUSE
Ankyrin repeat domain-containing pr...
Ankrd27
377Annotation score:
A0A0U1RPT7A0A0U1RPT7_MOUSE
Ankyrin repeat domain-containing pr...
Ankrd27
388Annotation score:
A0A087WPF2A0A087WPF2_MOUSE
Ankyrin repeat domain-containing pr...
Ankrd27
85Annotation score:
A0A0U1RP40A0A0U1RP40_MOUSE
Ankyrin repeat domain-containing pr...
Ankrd27
23Annotation score:
A0A0U1RPG1A0A0U1RPG1_MOUSE
Ankyrin repeat domain-containing pr...
Ankrd27
76Annotation score:

Sequence cautioni

The sequence AAP94281 differs from that shown. Reason: Frameshift at positions 920 and 929.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281 – 310QQKLL…TMCAD → TAEAAVPEEGGPAHDTISQP ESELGDHVCR in BAC39537 (PubMed:16141072).CuratedAdd BLAST30
Sequence conflicti371 – 372TP → PQ in BAD21367 (PubMed:15449545).Curated2
Sequence conflicti373 – 377DAEGF → VRSPC in AAH16493 (PubMed:15489334).Curated5
Sequence conflicti601P → Q in BAE26038 (PubMed:16141072).Curated1
Sequence conflicti609K → KV in BAD21367 (PubMed:15449545).Curated1
Sequence conflicti730S → P in AAP94281 (PubMed:16525121).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022791630 – 670Missing in isoform 3. 1 PublicationAdd BLAST41
Alternative sequenceiVSP_022792670 – 724Missing in isoform 2. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336500 mRNA Translation: AAP94281.1 Frameshift.
AK085796 mRNA Translation: BAC39537.1
AK144733 mRNA Translation: BAE26038.1
BC016493 mRNA Translation: AAH16493.1
BC065093 mRNA Translation: AAH65093.1
AK131117 Transcribed RNA Translation: BAD21367.1
CCDSiCCDS21154.1 [Q3UMR0-1]
RefSeqiNP_663608.3, NM_145633.3 [Q3UMR0-1]
NP_839994.1, NM_178263.3
UniGeneiMm.272620

Genome annotation databases

EnsembliENSMUST00000040844; ENSMUSP00000041751; ENSMUSG00000034867 [Q3UMR0-1]
ENSMUST00000190503; ENSMUSP00000140259; ENSMUSG00000034867 [Q3UMR0-2]
GeneIDi245886
KEGGimmu:245886
UCSCiuc009gkd.1 mouse [Q3UMR0-1]
uc012fin.1 mouse [Q3UMR0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336500 mRNA Translation: AAP94281.1 Frameshift.
AK085796 mRNA Translation: BAC39537.1
AK144733 mRNA Translation: BAE26038.1
BC016493 mRNA Translation: AAH16493.1
BC065093 mRNA Translation: AAH65093.1
AK131117 Transcribed RNA Translation: BAD21367.1
CCDSiCCDS21154.1 [Q3UMR0-1]
RefSeqiNP_663608.3, NM_145633.3 [Q3UMR0-1]
NP_839994.1, NM_178263.3
UniGeneiMm.272620

3D structure databases

ProteinModelPortaliQ3UMR0
SMRiQ3UMR0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232848, 1 interactor
IntActiQ3UMR0, 2 interactors
STRINGi10090.ENSMUSP00000041751

PTM databases

iPTMnetiQ3UMR0
PhosphoSitePlusiQ3UMR0

Proteomic databases

MaxQBiQ3UMR0
PaxDbiQ3UMR0
PRIDEiQ3UMR0

Protocols and materials databases

DNASUi245886
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040844; ENSMUSP00000041751; ENSMUSG00000034867 [Q3UMR0-1]
ENSMUST00000190503; ENSMUSP00000140259; ENSMUSG00000034867 [Q3UMR0-2]
GeneIDi245886
KEGGimmu:245886
UCSCiuc009gkd.1 mouse [Q3UMR0-1]
uc012fin.1 mouse [Q3UMR0-2]

Organism-specific databases

CTDi84079
MGIiMGI:2444103 Ankrd27

Phylogenomic databases

eggNOGiENOG410IQ81 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00930000150856
HOGENOMiHOG000033958
HOVERGENiHBG080845
InParanoidiQ3UMR0
KOiK20175
OrthoDBiEOG091G07HY
PhylomeDBiQ3UMR0
TreeFamiTF351261

Enzyme and pathway databases

ReactomeiR-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Miscellaneous databases

ChiTaRSiAnkrd27 mouse
PROiPR:Q3UMR0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034867 Expressed in 237 organ(s), highest expression level in embryo
CleanExiMM_ANKRD27
ExpressionAtlasiQ3UMR0 baseline and differential
GenevisibleiQ3UMR0 MM

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.20.1050.80, 1 hit
1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR003123 VPS9
IPR037191 VPS9_dom_sf
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF02204 VPS9, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 8 hits
SM00167 VPS9, 1 hit
SUPFAMiSSF109993 SSF109993, 1 hit
SSF48403 SSF48403, 2 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 8 hits
PS51205 VPS9, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiANR27_MOUSE
AccessioniPrimary (citable) accession number: Q3UMR0
Secondary accession number(s): Q6KAU0
, Q6P1F9, Q7TNY8, Q8BUD2, Q91W65
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: November 7, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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