UniProtKB - Q3UMM4 (CDK10_MOUSE)
Protein
Cyclin-dependent kinase 10
Gene
Cdk10
Organism
Mus musculus (Mouse)
Status
Functioni
Cyclin-dependent kinase that phosphorylates the transcription factor ETS2 (in vitro) and positively controls its proteasomal degradation (in cells). Involved in the regulation of actin cytoskeleton organization through the phosphorylation of actin dynamics regulators such as PKN2. Is a negative regulator of ciliogenesis through phosphorylation of PKN2 and promotion of RhoA signaling.By similarity
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 68 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 163 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 45 – 53 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cyclin-dependent protein kinase activity Source: MGI
- cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: RHEA
- protein threonine kinase activity Source: RHEA
GO - Biological processi
- cell projection organization Source: UniProtKB-KW
- negative regulation of cilium assembly Source: UniProtKB
- peptidyl-threonine phosphorylation Source: UniProtKB
- positive regulation of MAPK cascade Source: MGI
- protein phosphorylation Source: GO_Central
- regulation of actin cytoskeleton organization Source: UniProtKB
- regulation of mitotic cell cycle Source: GO_Central
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cilium biogenesis/degradation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclin-dependent kinase 10 (EC:2.7.11.22)Alternative name(s): Cell division protein kinase 10 |
Gene namesi | Name:Cdk10 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2448549, Cdk10 |
Subcellular locationi
Cytoskeleton
- cilium basal body By similarity
Cytoskeleton
- ciliary basal body Source: UniProtKB
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: UniProtKB-KW
Keywords - Cellular componenti
Cell projection, Cytoplasm, CytoskeletonPathology & Biotechi
Disruption phenotypei
CDK10 knockout results in partial prenatal lethality. Surviving mice display severe growth retardation, a reduced volume of mineralized matrix in the head, femur, tibia and fibula, bifidity or clefting of C1 (atlas) or C2 (axis), and absence of the dens. Additional defects are present in the kidney, lung, heart, spleen, liver, and muscle. At cellular level, CDK10 knockout does not affect cell proliferation. However, knocked-out mouse embryonic fibroblasts (MEFs) develop longer cilia.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000261028 | 1 – 360 | Cyclin-dependent kinase 10Add BLAST | 360 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 196 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q3UMM4 |
jPOSTi | Q3UMM4 |
MaxQBi | Q3UMM4 |
PaxDbi | Q3UMM4 |
PRIDEi | Q3UMM4 |
ProteomicsDBi | 280037 |
PTM databases
iPTMneti | Q3UMM4 |
PhosphoSitePlusi | Q3UMM4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000033862, Expressed in ear vesicle and 255 other tissues |
ExpressionAtlasi | Q3UMM4, baseline and differential |
Genevisiblei | Q3UMM4, MM |
Interactioni
Subunit structurei
Heterodimer with CCNQ, the interaction is required for kinase activity.
Interacts with ETS2.
Interacts with PRK2.
By similarityProtein-protein interaction databases
ComplexPortali | CPX-327, Cyclin M-CDK10 complex |
STRINGi | 10090.ENSMUSP00000045527 |
Miscellaneous databases
RNActi | Q3UMM4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 323 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 285 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0663, Eukaryota |
GeneTreei | ENSGT00940000158102 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | Q3UMM4 |
OMAi | MTDKGCI |
OrthoDBi | 925637at2759 |
PhylomeDBi | Q3UMM4 |
TreeFami | TF101026 |
Family and domain databases
CDDi | cd07845, STKc_CDK10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR044093, STKc_CDK10 |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q3UMM4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEVDLESDQ IRLKCIRKEG FFTVPPEHRL GRCRSVKEFE KLNRIGEGTY
60 70 80 90 100
GIVYRARDTQ TDEIVALKKV RMDKEKDGIP ISSLREITLL LRLRHPNIVE
110 120 130 140 150
LKEVVVGNHL ESIFLVMGYC EQDLASLLEN MPTPFSEAQV KCIMLQVLRG
160 170 180 190 200
LQYLHRNFII HRDLKVSNLL MTDKGCVKTA DFGLARAYGV PVKPMTPKVV
210 220 230 240 250
TLWYRAPELL LGTTTQTTSI DMWAVGCILA ELLAHKPLLP GTSEIHQIDL
260 270 280 290 300
IVQLLGTPSE NIWPGFSKLP LAGQYSLRKQ PYNNLKHKFP WLSEAGLRLL
310 320 330 340 350
NFLFMYDPKK RATSGDCLES SYFKEKPLPC EPELMPTFPH HRNKRAAPAA
360
AEGQSKRCRP
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1D5RLN0 | A0A1D5RLN0_MOUSE | Cyclin-dependent kinase 10 | Cdk10 | 289 | Annotation score: | ||
A0A1D5RM75 | A0A1D5RM75_MOUSE | Cyclin-dependent kinase 10 | Cdk10 | 140 | Annotation score: | ||
A0A1D5RLN1 | A0A1D5RLN1_MOUSE | Cyclin-dependent kinase 10 | Cdk10 | 129 | Annotation score: | ||
A0A1D5RLY3 | A0A1D5RLY3_MOUSE | Cyclin-dependent kinase 10 | Cdk10 | 115 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ288857 mRNA Translation: ABB96224.1 AK133918 mRNA Translation: BAE21925.1 AK144801 mRNA Translation: BAE26074.1 |
CCDSi | CCDS22751.1 |
RefSeqi | NP_919426.2, NM_194444.2 NP_919428.1, NM_194446.2 |
Genome annotation databases
Ensembli | ENSMUST00000036880; ENSMUSP00000045527; ENSMUSG00000033862 |
GeneIDi | 234854 |
KEGGi | mmu:234854 |
UCSCi | uc009nun.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ288857 mRNA Translation: ABB96224.1 AK133918 mRNA Translation: BAE21925.1 AK144801 mRNA Translation: BAE26074.1 |
CCDSi | CCDS22751.1 |
RefSeqi | NP_919426.2, NM_194444.2 NP_919428.1, NM_194446.2 |
3D structure databases
SMRi | Q3UMM4 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-327, Cyclin M-CDK10 complex |
STRINGi | 10090.ENSMUSP00000045527 |
PTM databases
iPTMneti | Q3UMM4 |
PhosphoSitePlusi | Q3UMM4 |
Proteomic databases
EPDi | Q3UMM4 |
jPOSTi | Q3UMM4 |
MaxQBi | Q3UMM4 |
PaxDbi | Q3UMM4 |
PRIDEi | Q3UMM4 |
ProteomicsDBi | 280037 |
Protocols and materials databases
Antibodypediai | 30892, 317 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000036880; ENSMUSP00000045527; ENSMUSG00000033862 |
GeneIDi | 234854 |
KEGGi | mmu:234854 |
UCSCi | uc009nun.2, mouse |
Organism-specific databases
CTDi | 8558 |
MGIi | MGI:2448549, Cdk10 |
Phylogenomic databases
eggNOGi | KOG0663, Eukaryota |
GeneTreei | ENSGT00940000158102 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | Q3UMM4 |
OMAi | MTDKGCI |
OrthoDBi | 925637at2759 |
PhylomeDBi | Q3UMM4 |
TreeFami | TF101026 |
Miscellaneous databases
BioGRID-ORCSi | 234854, 4 hits in 53 CRISPR screens |
ChiTaRSi | Cdk10, mouse |
PROi | PR:Q3UMM4 |
RNActi | Q3UMM4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000033862, Expressed in ear vesicle and 255 other tissues |
ExpressionAtlasi | Q3UMM4, baseline and differential |
Genevisiblei | Q3UMM4, MM |
Family and domain databases
CDDi | cd07845, STKc_CDK10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR044093, STKc_CDK10 |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDK10_MOUSE | |
Accessioni | Q3UMM4Primary (citable) accession number: Q3UMM4 Secondary accession number(s): Q3UZD2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | October 11, 2005 | |
Last modified: | April 7, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families