Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATPase family protein 2 homolog

Gene

Spata5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent chaperone which uses the energy provided by ATP hydrolysis to generate mechanical force to disassemble protein complexes (By similarity). May be involved in morphological and functional mitochondrial transformations during spermatogenesis (PubMed:10734318).By similarity1 Publication

Miscellaneous

Aberrantly expressed at the malignant conversion stage in a clonal epidermal model of chemical carcinogenesis.

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi394 – 401ATP 1By similarity8
Nucleotide bindingi668 – 675ATP 2By similarity8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionChaperone, Developmental protein, Hydrolase
Biological processDifferentiation, Spermatogenesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase family protein 2 homologCurated (EC:3.6.4.10By similarity)
Alternative name(s):
Spermatogenesis-associated factor protein
Spermatogenesis-associated protein 5
Gene namesi
Name:Spata5
Synonyms:Spaf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1927170 Spata5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003305841 – 893ATPase family protein 2 homologAdd BLAST893

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei271PhosphothreonineCombined sources1
Modified residuei273PhosphoserineBy similarity1
Modified residuei278PhosphoserineBy similarity1
Cross-linki859Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ3UMC0
MaxQBiQ3UMC0
PaxDbiQ3UMC0
PRIDEiQ3UMC0

PTM databases

iPTMnetiQ3UMC0
PhosphoSitePlusiQ3UMC0

Expressioni

Tissue specificityi

Expressed in testis; with localization to spermatogonia and early spermatocytes in the basal compartment of the seminiferous tubules (at early stages of spermatogenesis). Very weak signals observed in spleen and skin.1 Publication

Gene expression databases

BgeeiENSMUSG00000027722 Expressed in 278 organ(s), highest expression level in female gonad
ExpressionAtlasiQ3UMC0 baseline and differential
GenevisibleiQ3UMC0 MM

Interactioni

Subunit structurei

Homohexamer; ATP binding induces oligomerization. Forms a ring-shaped particle of about 12 nm diameter, that displays 6-fold radial symmetry.By similarity

Protein-protein interaction databases

BioGridi208337, 2 interactors
IntActiQ3UMC0, 1 interactor
STRINGi10090.ENSMUSP00000103747

Structurei

3D structure databases

ProteinModelPortaliQ3UMC0
SMRiQ3UMC0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The first ATP-binding region binds ATP with low affinity whereas the second ATP-binding region binds ATP with high affinity.By similarity

Sequence similaritiesi

Belongs to the AAA ATPase family. AFG2 subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0730 Eukaryota
COG0464 LUCA
GeneTreeiENSGT00900000140994
HOGENOMiHOG000223225
HOVERGENiHBG108504
InParanoidiQ3UMC0
KOiK14575
OMAiEVCTAWP
OrthoDBiEOG091G03VY
PhylomeDBiQ3UMC0
TreeFamiTF314525

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR009010 Asp_de-COase-like_dom_sf
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF50692 SSF50692, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00674 AAA, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q3UMC0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSKKNRKRQ KEGAEGASPS LSAAPSRSGT STPFAQPSPA APGMLVVTNF
60 70 80 90 100
LEKVDGKVPK TFQNSLVHLG LNTMKSANIC IGRPVLLTSL DGKQEVYTAW
110 120 130 140 150
PVAGFPGGKV GLSEMAQKNV GVRAGETIQV QPLLGAVLQA QEMDLALSDK
160 170 180 190 200
DKEINEEELT GCILRKLDGK IVLPGNFLYC TFYGRLCKLQ VLQVKGTDGT
210 220 230 240 250
TLGKLQSASG TDAQGMASEH SSMESSDVDL SFQLSQLDLK EPQSPSSQST
260 270 280 290 300
PCKPTNDRTV NKAGEVLLDV TQSPRDGSGL GLEESTGLKC SFDSSKEGNT
310 320 330 340 350
QPVSEEKLLK PASAGTKSNT DTFYFISSTT RINLRKICTN SKEQDSQFKV
360 370 380 390 400
TYDMIGGLNS QLKAIREIIE LPLKQPELFK SYGIPAPRGL LLYGPPGTGK
410 420 430 440 450
TMIARAVANE VGAYVSVING PEIISKFYGE TEARLRQIFA EATLRHPSII
460 470 480 490 500
FIDELDALCP KREGAQSEVE KRVVASLLTL MDGIGSEGSE GRVLVLGATN
510 520 530 540 550
RPQALDAALR RPGRFDKEIE IGIPNAQDRL DILQKLLRRV PHLLTKAELL
560 570 580 590 600
RLANNAHGYV GADLKALCNE AGLHALRRVL RKQPNLPDSK VAGMVKITLN
610 620 630 640 650
DFLQGMNDIR PSAMREVAID VPNVSWSDIG GLENIKLKLK QAVEWPLKHP
660 670 680 690 700
KSFNRMGIQP PKGVLLYGPP GCSKTMIAKA LANESGLNFL AIKGPELMNK
710 720 730 740 750
YVGESERAVR EIFRKARAVA PSIIFFDELD ALAVERGSSS GAGNVADRVL
760 770 780 790 800
AQLLTEMDGI EQLKNVTVLA ATNRPDRIDK ALMRPGRIDR IIYVPLPDAA
810 820 830 840 850
TRREILNLQF HSMPISNEVD LDELVLQTDT YSGAEIIAVC KEAALLALEE
860 870 880 890
NIKADCIMKR HFTQALSIVT PRIPESLRRF YEDYQEKSGL HTV
Length:893
Mass (Da):97,256
Last modified:April 29, 2008 - v2
Checksum:i1B5FF96EDC9B9596
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MQ80A0A0A0MQ80_MOUSE
ATPase family protein 2 homolog
Spata5
892Annotation score:
A0A0G2JFY0A0A0G2JFY0_MOUSE
ATPase family protein 2 homolog
Spata5
842Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54Missing in AAD02481 (PubMed:10734318).Curated1
Sequence conflicti54Missing in CAM46306 (PubMed:19468303).Curated1
Sequence conflicti54Missing in CAM21041 (PubMed:19468303).Curated1
Sequence conflicti205L → P in AAD02481 (PubMed:10734318).Curated1
Sequence conflicti205L → P in BAE26178 (PubMed:16141072).Curated1
Sequence conflicti205L → P in CAM46305 (PubMed:19468303).Curated1
Sequence conflicti205L → P in CAM46306 (PubMed:19468303).Curated1
Sequence conflicti225S → N in AAD02481 (PubMed:10734318).Curated1
Sequence conflicti225S → N in CAM46305 (PubMed:19468303).Curated1
Sequence conflicti225S → N in CAM46306 (PubMed:19468303).Curated1
Sequence conflicti864Q → E in AAD02481 (PubMed:10734318).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049099 mRNA Translation: AAD02481.1
AK011111 mRNA Translation: BAB27406.1
AK144998 mRNA Translation: BAE26178.1
AL669927, AL627074 Genomic DNA Translation: CAM21041.1
AL669927, AL627074 Genomic DNA Translation: CAM21042.1
AL627074, AL669927 Genomic DNA Translation: CAM25218.1
AL627074, AL669927 Genomic DNA Translation: CAM25219.1
AL645968 Genomic DNA Translation: CAM46305.1
AL645968 Genomic DNA Translation: CAM46306.1
CCDSiCCDS50895.1
RefSeqiNP_001156983.1, NM_001163511.2
NP_001297402.1, NM_001310473.1
NP_067318.2, NM_021343.3
UniGeneiMm.172679

Genome annotation databases

EnsembliENSMUST00000108112; ENSMUSP00000103747; ENSMUSG00000027722
GeneIDi57815
KEGGimmu:57815
UCSCiuc008pav.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049099 mRNA Translation: AAD02481.1
AK011111 mRNA Translation: BAB27406.1
AK144998 mRNA Translation: BAE26178.1
AL669927, AL627074 Genomic DNA Translation: CAM21041.1
AL669927, AL627074 Genomic DNA Translation: CAM21042.1
AL627074, AL669927 Genomic DNA Translation: CAM25218.1
AL627074, AL669927 Genomic DNA Translation: CAM25219.1
AL645968 Genomic DNA Translation: CAM46305.1
AL645968 Genomic DNA Translation: CAM46306.1
CCDSiCCDS50895.1
RefSeqiNP_001156983.1, NM_001163511.2
NP_001297402.1, NM_001310473.1
NP_067318.2, NM_021343.3
UniGeneiMm.172679

3D structure databases

ProteinModelPortaliQ3UMC0
SMRiQ3UMC0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208337, 2 interactors
IntActiQ3UMC0, 1 interactor
STRINGi10090.ENSMUSP00000103747

PTM databases

iPTMnetiQ3UMC0
PhosphoSitePlusiQ3UMC0

Proteomic databases

EPDiQ3UMC0
MaxQBiQ3UMC0
PaxDbiQ3UMC0
PRIDEiQ3UMC0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108112; ENSMUSP00000103747; ENSMUSG00000027722
GeneIDi57815
KEGGimmu:57815
UCSCiuc008pav.2 mouse

Organism-specific databases

CTDi166378
MGIiMGI:1927170 Spata5

Phylogenomic databases

eggNOGiKOG0730 Eukaryota
COG0464 LUCA
GeneTreeiENSGT00900000140994
HOGENOMiHOG000223225
HOVERGENiHBG108504
InParanoidiQ3UMC0
KOiK14575
OMAiEVCTAWP
OrthoDBiEOG091G03VY
PhylomeDBiQ3UMC0
TreeFamiTF314525

Miscellaneous databases

PROiPR:Q3UMC0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027722 Expressed in 278 organ(s), highest expression level in female gonad
ExpressionAtlasiQ3UMC0 baseline and differential
GenevisibleiQ3UMC0 MM

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR009010 Asp_de-COase-like_dom_sf
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF50692 SSF50692, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00674 AAA, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiAFG2H_MOUSE
AccessioniPrimary (citable) accession number: Q3UMC0
Secondary accession number(s): A2ACU2
, A3KG23, A3KG24, Q9CXZ7, Q9Z2K7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: November 7, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again