UniProtKB - Q3UL97 (MCAF2_MOUSE)
Protein
Activating transcription factor 7-interacting protein 2
Gene
Atf7ip2
Organism
Mus musculus (Mouse)
Status
Functioni
Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) activity (By similarity).By similarity
Keywordsi
Molecular function | Activator, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Activating transcription factor 7-interacting protein 2Alternative name(s): MBD1-containing chromatin-associated factor 2 Protein similar to MCAF2 |
Gene namesi | Name:Atf7ip2 Synonyms:Mcaf2, Psm2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1922579, Atf7ip2 |
Subcellular locationi
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281785 | 1 – 452 | Activating transcription factor 7-interacting protein 2Add BLAST | 452 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 184 | PhosphoserineBy similarity | 1 | |
Modified residuei | 257 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q3UL97 |
PRIDEi | Q3UL97 |
PTM databases
PhosphoSitePlusi | Q3UL97 |
Expressioni
Tissue specificityi
Expressed in testis.1 Publication
Developmental stagei
Expressed in early mouse embryo, especially in the embryonic gonad from 11.5 dpc. Continuously expressed from newborn testis to adult.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000039200, Expressed in spermatocyte and 116 other tissues |
ExpressionAtlasi | Q3UL97, baseline and differential |
Genevisiblei | Q3UL97, MM |
Interactioni
Subunit structurei
Interacts with MBD1, SETDB1 and SP1. Probably forms a complex with SETDB1 and MBD1 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000036731 |
Miscellaneous databases
RNActi | Q3UL97, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 346 – 450 | Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST | 105 |
Sequence similaritiesi
Belongs to the MCAF family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502S2DM, Eukaryota |
GeneTreei | ENSGT00530000063707 |
HOGENOMi | CLU_025197_0_0_1 |
InParanoidi | Q3UL97 |
OMAi | TCHLSQN |
OrthoDBi | 646436at2759 |
PhylomeDBi | Q3UL97 |
TreeFami | TF329427 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR026085, ATF7-int IPR026089, ATF7-int_2 IPR031870, ATF7IP_BD IPR003961, FN3_dom IPR036116, FN3_sf IPR013783, Ig-like_fold |
PANTHERi | PTHR23210, PTHR23210, 2 hits PTHR23210:SF23, PTHR23210:SF23, 2 hits |
Pfami | View protein in Pfam PF16788, ATF7IP_BD, 1 hit PF16794, fn3_4, 1 hit |
SUPFAMi | SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q3UL97-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MESPDRKRQK VLKAKKTMPT SYQKQLEILN KSTNVEAPKT TVGTNIPNGH
60 70 80 90 100
NQKMFSKNKE NVKVMKVSEQ INENACGALE RHTALLEQVK HWIRQEICMI
110 120 130 140 150
NCNLFDKKLN ELNERIGKTQ CKSRHEAIAG ELFVKIRRLQ KRIKTVLSSQ
160 170 180 190 200
RNCLEPNTLP SNTVCKVTDS EAMNLNVTQK SVKSRSKRIS SVNHTPLNSS
210 220 230 240 250
EKAGRKTNLP STCVEFASES NTDDVMLISV KNSNLTTSIT SEQTEIRKNT
260 270 280 290 300
SRNLSNSPNS MIKVGPVEKK FDFVIDLTRE GPSNYSIESP SFTLKSTSKA
310 320 330 340 350
VLRSKEIIPV AENGNEGFGS FEHLPPLPEP PAPLPEMADK IKDTLPPQKP
360 370 380 390 400
ELKVKWVLRP TSIALTWNIP KVNPNCAPVE SYHLFLYYEN SDHLTWKKIA
410 420 430 440 450
EIKALPLPMA CTLSQNLAST KYYFAVQSKD IFGRYGPFCN IKSIPRFSEN
LT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2R8W6K8 | A0A2R8W6K8_MOUSE | Activating transcription factor 7-i... | Atf7ip2 | 133 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 18 | M → V in AAX99226 (PubMed:16850184).Curated | 1 | |
Sequence conflicti | 21 | S → G in AAX99226 (PubMed:16850184).Curated | 1 | |
Sequence conflicti | 97 | I → V in AAX99226 (PubMed:16850184).Curated | 1 | |
Sequence conflicti | 188 | R → T in BAE24164 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 231 – 233 | KNS → NFF in BAB30138 (PubMed:16141072).Curated | 3 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024044 | 204 – 225 | GRKTN…NTDDV → AGFENCGQSPDQFLVPNNSL LT in isoform 4. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_024045 | 226 – 452 | Missing in isoform 4. 1 PublicationAdd BLAST | 227 | |
Alternative sequenceiVSP_024046 | 288 – 300 | ESPSF…STSKA → GKGCFYIFLSKCM in isoform 3. 1 PublicationAdd BLAST | 13 | |
Alternative sequenceiVSP_024047 | 301 – 452 | Missing in isoform 3. 1 PublicationAdd BLAST | 152 | |
Alternative sequenceiVSP_024048 | 317 – 319 | GFG → VLT in isoform 2. 2 Publications | 3 | |
Alternative sequenceiVSP_024049 | 320 – 452 | Missing in isoform 2. 2 PublicationsAdd BLAST | 133 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY903215 mRNA Translation: AAX99226.1 AK016180 mRNA Translation: BAB30138.1 AK077229 mRNA Translation: BAC36698.1 AK139862 mRNA Translation: BAE24164.1 AK145634 mRNA Translation: BAE26553.1 |
CCDSi | CCDS49758.1 [Q3UL97-1] CCDS49759.1 [Q3UL97-3] |
RefSeqi | NP_083529.1, NM_029253.1 [Q3UL97-3] NP_694763.2, NM_153123.2 [Q3UL97-1] |
Genome annotation databases
Ensembli | ENSMUST00000044005; ENSMUSP00000036731; ENSMUSG00000039200 [Q3UL97-1] ENSMUST00000100191; ENSMUSP00000097766; ENSMUSG00000039200 [Q3UL97-4] ENSMUST00000117220; ENSMUSP00000113573; ENSMUSG00000039200 [Q3UL97-2] ENSMUST00000119023; ENSMUSP00000113480; ENSMUSG00000039200 [Q3UL97-3] |
GeneIDi | 75329 |
KEGGi | mmu:75329 |
UCSCi | uc007ydf.2, mouse [Q3UL97-3] uc007ydh.2, mouse [Q3UL97-2] uc007ydi.2, mouse [Q3UL97-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY903215 mRNA Translation: AAX99226.1 AK016180 mRNA Translation: BAB30138.1 AK077229 mRNA Translation: BAC36698.1 AK139862 mRNA Translation: BAE24164.1 AK145634 mRNA Translation: BAE26553.1 |
CCDSi | CCDS49758.1 [Q3UL97-1] CCDS49759.1 [Q3UL97-3] |
RefSeqi | NP_083529.1, NM_029253.1 [Q3UL97-3] NP_694763.2, NM_153123.2 [Q3UL97-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000036731 |
PTM databases
PhosphoSitePlusi | Q3UL97 |
Proteomic databases
PaxDbi | Q3UL97 |
PRIDEi | Q3UL97 |
Protocols and materials databases
Antibodypediai | 52153, 85 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000044005; ENSMUSP00000036731; ENSMUSG00000039200 [Q3UL97-1] ENSMUST00000100191; ENSMUSP00000097766; ENSMUSG00000039200 [Q3UL97-4] ENSMUST00000117220; ENSMUSP00000113573; ENSMUSG00000039200 [Q3UL97-2] ENSMUST00000119023; ENSMUSP00000113480; ENSMUSG00000039200 [Q3UL97-3] |
GeneIDi | 75329 |
KEGGi | mmu:75329 |
UCSCi | uc007ydf.2, mouse [Q3UL97-3] uc007ydh.2, mouse [Q3UL97-2] uc007ydi.2, mouse [Q3UL97-1] |
Organism-specific databases
CTDi | 80063 |
MGIi | MGI:1922579, Atf7ip2 |
Phylogenomic databases
eggNOGi | ENOG502S2DM, Eukaryota |
GeneTreei | ENSGT00530000063707 |
HOGENOMi | CLU_025197_0_0_1 |
InParanoidi | Q3UL97 |
OMAi | TCHLSQN |
OrthoDBi | 646436at2759 |
PhylomeDBi | Q3UL97 |
TreeFami | TF329427 |
Miscellaneous databases
BioGRID-ORCSi | 75329, 2 hits in 17 CRISPR screens |
PROi | PR:Q3UL97 |
RNActi | Q3UL97, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039200, Expressed in spermatocyte and 116 other tissues |
ExpressionAtlasi | Q3UL97, baseline and differential |
Genevisiblei | Q3UL97, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR026085, ATF7-int IPR026089, ATF7-int_2 IPR031870, ATF7IP_BD IPR003961, FN3_dom IPR036116, FN3_sf IPR013783, Ig-like_fold |
PANTHERi | PTHR23210, PTHR23210, 2 hits PTHR23210:SF23, PTHR23210:SF23, 2 hits |
Pfami | View protein in Pfam PF16788, ATF7IP_BD, 1 hit PF16794, fn3_4, 1 hit |
SUPFAMi | SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCAF2_MOUSE | |
Accessioni | Q3UL97Primary (citable) accession number: Q3UL97 Secondary accession number(s): Q3UT16 Q9D4T8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | October 11, 2005 | |
Last modified: | February 10, 2021 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families