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Protein

Polycomb group RING finger protein 5

Gene

Pcgf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:27136092, PubMed:28596365). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (By similarity). Plays a redundant role with PCGF3 as part of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (PubMed:28596365).By similarity2 Publications

Miscellaneous

Selective knockout in hematopoietic stem cells has only minor effects on gene expression and hematopoiesis, probably due to functional compensation by Pcgf1.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri18 – 57RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb group RING finger protein 5
Alternative name(s):
RING finger protein 159
Gene namesi
Name:Pcgf5
Synonyms:Rnf159
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1923505 Pcgf5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Combined disruption of both Pcgf3 and Pcgf5 causes embryonic lethality; there are no live progeny. Male embryos are detected at 9.5 and 10.5 dpc, but are smaller than normal. Female embryos are already extensively degraded at 9.5 dpc. Placentas from male embryos have some parietal trophoblast giant cells, but fail to form a labyrinth. Placentas from female embryos lack trophoblasts altogether and fail to form a labyrinth. Defects can be attributed to the observed lack of monoubiquitination of histone H2A 'Lys-119' and lack of Xist-mediated silencing of one copy of the X chromosome.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002778691 – 256Polycomb group RING finger protein 5Add BLAST256

Proteomic databases

MaxQBiQ3UK78
PRIDEiQ3UK78

PTM databases

iPTMnetiQ3UK78
PhosphoSitePlusiQ3UK78

Expressioni

Tissue specificityi

Detected in hematopoietic stem cells and multipotent progenitor cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000024805 Expressed in 237 organ(s), highest expression level in placenta labyrinth
CleanExiMM_PCGF5
ExpressionAtlasiQ3UK78 baseline and differential
GenevisibleiQ3UK78 MM

Interactioni

Subunit structurei

Component of a PRC1-like complex that contains PCGF5, RNF2 and UBE2D3 (PubMed:28596365). Interacts with RNF2; the interaction is direct (PubMed:27136092, PubMed:28596365). Interacts with CBX6, CBX7 and CBX8. Interacts with AUTS2; the interaction is direct. Identified in a complex that contains AUTS2, PCGF5, CSNK2B and RNF2 (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi217946, 3 interactors
IntActiQ3UK78, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliQ3UK78
SMRiQ3UK78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri18 – 57RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000074463
HOGENOMiHOG000231946
HOVERGENiHBG052826
InParanoidiQ3UK78
KOiK11489
OMAiGEIMGRD
OrthoDBiEOG091G0EC9
PhylomeDBiQ3UK78
TreeFamiTF324206

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR039642 E3_Ub_ligase/polycomb_RING
IPR032443 RAWUL
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR10825 PTHR10825, 1 hit
PfamiView protein in Pfam
PF16207 RAWUL, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q3UK78-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATQRKHLVK DFNPYITCYI CKGYLIKPTT VTECLHTFCK TCIVQHFEDS
60 70 80 90 100
NDCPRCGNQV HETNPLEMLR LDNTLEEIIF KLVPGLREQE LQRELEFWKK
110 120 130 140 150
NKPQENGQDD ISKVDKSKAD EEGDENQDDK DYHRSDPQIA ICLDCLRNNG
160 170 180 190 200
QSGDNVVKGL MKKFIRCSTR VTVGTIKKFL SLKLKLPSSY ELDVLCNGEI
210 220 230 240 250
MGKDHTMEFI YMTRWRLRGE NLCCLNCSAS QVCSQDGTLY QSYPMVLQYR

PRIDFG
Length:256
Mass (Da):29,657
Last modified:October 11, 2005 - v1
Checksum:i053CF6DDEA3C2339
GO
Isoform 2 (identifier: Q3UK78-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-241: Missing.

Show »
Length:236
Mass (Da):27,539
Checksum:i430A370628CAFA19
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZP24B7ZP24_MOUSE
Pcgf5 protein
Pcgf5
255Annotation score:
A0A286YDQ6A0A286YDQ6_MOUSE
Polycomb group RING finger protein ...
Pcgf5
125Annotation score:
A0A286YD06A0A286YD06_MOUSE
Polycomb group RING finger protein ...
Pcgf5
132Annotation score:

Sequence cautioni

The sequence BAB22061 differs from that shown. Reason: Frameshift at position 201.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023120222 – 241Missing in isoform 2. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002387 mRNA Translation: BAB22061.1 Frameshift.
AK017984 mRNA Translation: BAB31023.1
AK146133 mRNA Translation: BAE26924.1
BC139258 mRNA Translation: AAI39259.1
BC139259 mRNA Translation: AAI39260.1
BC145590 mRNA Translation: AAI45591.1
CCDSiCCDS29772.1 [Q3UK78-2]
RefSeqiNP_083784.1, NM_029508.3 [Q3UK78-2]
XP_006527494.1, XM_006527431.3 [Q3UK78-1]
XP_006527496.1, XM_006527433.3 [Q3UK78-2]
XP_011245693.1, XM_011247391.2 [Q3UK78-1]
XP_011245694.1, XM_011247392.2 [Q3UK78-1]
XP_011245697.1, XM_011247395.2 [Q3UK78-2]
UniGeneiMm.259180
Mm.381165
Mm.488093
Mm.491199

Genome annotation databases

EnsembliENSMUST00000062389; ENSMUSP00000058730; ENSMUSG00000024805 [Q3UK78-2]
ENSMUST00000071267; ENSMUSP00000071245; ENSMUSG00000024805 [Q3UK78-2]
ENSMUST00000224679; ENSMUSP00000153681; ENSMUSG00000024805 [Q3UK78-1]
ENSMUST00000225411; ENSMUSP00000153464; ENSMUSG00000024805 [Q3UK78-1]
ENSMUST00000225920; ENSMUSP00000153206; ENSMUSG00000024805 [Q3UK78-2]
GeneIDi76073
KEGGimmu:76073
UCSCiuc008hhi.1 mouse [Q3UK78-2]
uc008hhk.1 mouse [Q3UK78-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002387 mRNA Translation: BAB22061.1 Frameshift.
AK017984 mRNA Translation: BAB31023.1
AK146133 mRNA Translation: BAE26924.1
BC139258 mRNA Translation: AAI39259.1
BC139259 mRNA Translation: AAI39260.1
BC145590 mRNA Translation: AAI45591.1
CCDSiCCDS29772.1 [Q3UK78-2]
RefSeqiNP_083784.1, NM_029508.3 [Q3UK78-2]
XP_006527494.1, XM_006527431.3 [Q3UK78-1]
XP_006527496.1, XM_006527433.3 [Q3UK78-2]
XP_011245693.1, XM_011247391.2 [Q3UK78-1]
XP_011245694.1, XM_011247392.2 [Q3UK78-1]
XP_011245697.1, XM_011247395.2 [Q3UK78-2]
UniGeneiMm.259180
Mm.381165
Mm.488093
Mm.491199

3D structure databases

ProteinModelPortaliQ3UK78
SMRiQ3UK78
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217946, 3 interactors
IntActiQ3UK78, 1 interactor

PTM databases

iPTMnetiQ3UK78
PhosphoSitePlusiQ3UK78

Proteomic databases

MaxQBiQ3UK78
PRIDEiQ3UK78

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062389; ENSMUSP00000058730; ENSMUSG00000024805 [Q3UK78-2]
ENSMUST00000071267; ENSMUSP00000071245; ENSMUSG00000024805 [Q3UK78-2]
ENSMUST00000224679; ENSMUSP00000153681; ENSMUSG00000024805 [Q3UK78-1]
ENSMUST00000225411; ENSMUSP00000153464; ENSMUSG00000024805 [Q3UK78-1]
ENSMUST00000225920; ENSMUSP00000153206; ENSMUSG00000024805 [Q3UK78-2]
GeneIDi76073
KEGGimmu:76073
UCSCiuc008hhi.1 mouse [Q3UK78-2]
uc008hhk.1 mouse [Q3UK78-1]

Organism-specific databases

CTDi84333
MGIiMGI:1923505 Pcgf5

Phylogenomic databases

GeneTreeiENSGT00550000074463
HOGENOMiHOG000231946
HOVERGENiHBG052826
InParanoidiQ3UK78
KOiK11489
OMAiGEIMGRD
OrthoDBiEOG091G0EC9
PhylomeDBiQ3UK78
TreeFamiTF324206

Enzyme and pathway databases

ReactomeiR-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known

Miscellaneous databases

PROiPR:Q3UK78
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024805 Expressed in 237 organ(s), highest expression level in placenta labyrinth
CleanExiMM_PCGF5
ExpressionAtlasiQ3UK78 baseline and differential
GenevisibleiQ3UK78 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR039642 E3_Ub_ligase/polycomb_RING
IPR032443 RAWUL
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR10825 PTHR10825, 1 hit
PfamiView protein in Pfam
PF16207 RAWUL, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPCGF5_MOUSE
AccessioniPrimary (citable) accession number: Q3UK78
Secondary accession number(s): B2RTD3
, B7ZP23, Q9D3E5, Q9DCW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2005
Last modified: November 7, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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