UniProtKB - Q3UJF0 (GPR55_MOUSE)
Protein
G-protein coupled receptor 55
Gene
Gpr55
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca2+ release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA (By similarity). Putative cannabinoid receptor (By similarity). May play a role in bone physiology by regulating osteoclast number and function (By similarity). May be involved in hyperalgesia associated with inflammatory and neuropathic pain.By similarity1 Publication
Miscellaneous
The classification of this protein as a cannabinoid receptor remains a contentious issue due to conflicting pharmacological results.
GO - Molecular functioni
- cannabinoid receptor activity Source: MGI
- G protein-coupled receptor activity Source: GO_Central
GO - Biological processi
- activation of phospholipase C activity Source: UniProtKB
- bone resorption Source: MGI
- negative regulation of osteoclast differentiation Source: MGI
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway Source: GO_Central
- positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
- positive regulation of Rho protein signal transduction Source: UniProtKB
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-373076, Class A/1 (Rhodopsin-like receptors) R-MMU-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: G-protein coupled receptor 55 |
Gene namesi | Name:Gpr55 Synonyms:Gm218 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2685064, Gpr55 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 20 | ExtracellularSequence analysisAdd BLAST | 20 | |
Transmembranei | 21 – 41 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 42 – 57 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 58 – 78 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 79 – 93 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 94 – 114 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 115 – 136 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 137 – 157 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 158 – 179 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 180 – 200 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 201 – 239 | CytoplasmicSequence analysisAdd BLAST | 39 | |
Transmembranei | 240 – 260 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 261 – 279 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 280 – 300 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 301 – 327 | CytoplasmicSequence analysisAdd BLAST | 27 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutant mice are resistant to mechanical hyperalgesia and have increased levels of anti-inflammatory cytokines.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000233978 | 1 – 327 | G-protein coupled receptor 55Add BLAST | 327 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 8 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 170 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q3UJF0 |
PRIDEi | Q3UJF0 |
PTM databases
GlyGeni | Q3UJF0, 2 sites |
iPTMneti | Q3UJF0 |
PhosphoSitePlusi | Q3UJF0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000049608, Expressed in duodenum and 38 other tissues |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000084196 |
Miscellaneous databases
RNActi | Q3UJF0, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QWNM, Eukaryota |
GeneTreei | ENSGT01010000222244 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q3UJF0 |
OMAi | FHNMSDG |
OrthoDBi | 981789at2759 |
TreeFami | TF335700 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR028334, GPR55 |
PANTHERi | PTHR24232:SF56, PTHR24232:SF56, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q3UJF0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQPERDNCS FDSVDKLTRT LQLAVHIPTF LLGLVLNLLA IRGFSAFLKK
60 70 80 90 100
RKLDYIATSI YMINLAVFDL LLVLSLPFKM VLPQVESPLP SFCTLVECLY
110 120 130 140 150
FISMYGSVFT ICFISLDRFL AIQYPILASH LRSPRKTFGI CCIIWMLVWI
160 170 180 190 200
GSIPIYTFHR EVERYKCFHN MSDVTWSASV FFPLEIFGFL LPMGIMGFCS
210 220 230 240 250
YRSIHILLRR PDSTEDWVQQ RDTKGWVQKR ACIWTIATNL VIFVVSFLPV
260 270 280 290 300
HLGFFLQYLV RNRFILDCRM KQGISLFLQL SLCFSNINCC LDVFCYYFVI
310 320
KEFRMRIKAH RPSTIKLVNQ DTMVSRG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 226 – 231 | WVQKRA → LGTKREP in BAE27205 (PubMed:16141072).Curated | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK146484 mRNA Translation: BAE27205.1 AC102506 Genomic DNA No translation available. AC107707 Genomic DNA No translation available. |
CCDSi | CCDS15115.1 |
RefSeqi | NP_001028462.2, NM_001033290.2 XP_006529526.1, XM_006529463.3 XP_017175826.1, XM_017320337.1 |
Genome annotation databases
Ensembli | ENSMUST00000086975; ENSMUSP00000084196; ENSMUSG00000049608 |
GeneIDi | 227326 |
KEGGi | mmu:227326 |
UCSCi | uc007buq.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK146484 mRNA Translation: BAE27205.1 AC102506 Genomic DNA No translation available. AC107707 Genomic DNA No translation available. |
CCDSi | CCDS15115.1 |
RefSeqi | NP_001028462.2, NM_001033290.2 XP_006529526.1, XM_006529463.3 XP_017175826.1, XM_017320337.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000084196 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q3UJF0, 2 sites |
iPTMneti | Q3UJF0 |
PhosphoSitePlusi | Q3UJF0 |
Proteomic databases
PaxDbi | Q3UJF0 |
PRIDEi | Q3UJF0 |
Protocols and materials databases
Antibodypediai | 20197, 173 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000086975; ENSMUSP00000084196; ENSMUSG00000049608 |
GeneIDi | 227326 |
KEGGi | mmu:227326 |
UCSCi | uc007buq.1, mouse |
Organism-specific databases
CTDi | 9290 |
MGIi | MGI:2685064, Gpr55 |
Phylogenomic databases
eggNOGi | ENOG502QWNM, Eukaryota |
GeneTreei | ENSGT01010000222244 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q3UJF0 |
OMAi | FHNMSDG |
OrthoDBi | 981789at2759 |
TreeFami | TF335700 |
Enzyme and pathway databases
Reactomei | R-MMU-373076, Class A/1 (Rhodopsin-like receptors) R-MMU-418594, G alpha (i) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 227326, 0 hits in 17 CRISPR screens |
PROi | PR:Q3UJF0 |
RNActi | Q3UJF0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000049608, Expressed in duodenum and 38 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR028334, GPR55 |
PANTHERi | PTHR24232:SF56, PTHR24232:SF56, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPR55_MOUSE | |
Accessioni | Q3UJF0Primary (citable) accession number: Q3UJF0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2006 |
Last sequence update: | October 3, 2012 | |
Last modified: | December 2, 2020 | |
This is version 105 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries