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Protein

Nucleolar protein 8

Gene

Nol8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in the survival of diffuse-type gastric cancer cells. Acts as a nucleolar anchoring protein for DDX47. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processrRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 8
Gene namesi
Name:Nol8Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1918180 Nol8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002394441 – 1147Nucleolar protein 8Add BLAST1147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki225Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei300PhosphoserineCombined sources1
Modified residuei306PhosphoserineCombined sources1
Cross-linki316Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei362PhosphotyrosineBy similarity1
Modified residuei364PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei367PhosphothreonineCombined sources1
Modified residuei416PhosphoserineBy similarity1
Modified residuei704PhosphoserineBy similarity1
Modified residuei777PhosphothreonineCombined sources1
Modified residuei783PhosphoserineCombined sources1
Modified residuei787PhosphoserineCombined sources1
Modified residuei819PhosphoserineCombined sources1
Modified residuei820PhosphoserineCombined sources1
Modified residuei825PhosphoserineCombined sources1
Modified residuei827PhosphoserineBy similarity1
Modified residuei872PhosphoserineBy similarity1
Cross-linki1038Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1063PhosphoserineCombined sources1
Modified residuei1064PhosphoserineCombined sources1
Modified residuei1065PhosphoserineCombined sources1
Modified residuei1080PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ3UHX0
MaxQBiQ3UHX0
PaxDbiQ3UHX0
PeptideAtlasiQ3UHX0
PRIDEiQ3UHX0

PTM databases

iPTMnetiQ3UHX0
PhosphoSitePlusiQ3UHX0

Expressioni

Gene expression databases

CleanExiMM_NOL8
ExpressionAtlasiQ3UHX0 baseline and differential

Interactioni

Subunit structurei

Interacts with the GTP form of RRAGA, RRAGC and RRAGD. Interacts with NIP7. Interacts with DDX18; the interaction is RNA-dependent. Interacts with DDX47; the interaction is RNA-dependent (By similarity).By similarity

Protein-protein interaction databases

IntActiQ3UHX0, 1 interactor
MINTiQ3UHX0
STRINGi10090.ENSMUSP00000021824

Structurei

3D structure databases

ProteinModelPortaliQ3UHX0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 89RRMPROSITE-ProRule annotationAdd BLAST82

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili868 – 898Sequence analysisAdd BLAST31
Coiled coili937 – 963Sequence analysisAdd BLAST27

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1093 – 1096Poly-PheSequence analysis4

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4365 Eukaryota
ENOG410Y5CN LUCA
GeneTreeiENSGT00390000004860
HOGENOMiHOG000293312
HOVERGENiHBG057565
InParanoidiQ3UHX0
OMAiHYDPTRH
PhylomeDBiQ3UHX0

Family and domain databases

CDDicd12226 RRM_NOL8, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR034138 NOP8_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 11 Publication (identifier: Q3UHX0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MQGNREMKRL FVGGLGQGIS ETDLQNQFGR FGEVSDVEII TRKDDQGNSQ
60 70 80 90 100
KVFAYVNIQI TEADLKKCMS ILNKTKWKGG TLQIQLAKES FLHRLAQERE
110 120 130 140 150
DAKAKKEKST TGNPTLLEKM GAVDFHMKAV PGTEVPGHKN WVVSKFGRVL
160 170 180 190 200
PVLHLKNQQK HKIMKYDPSK YCHNIKKIPE NLTETTPIAE LTWELEGGND
210 220 230 240 250
PMSKKRRGEF SDFHIPPQKV KKVQKSNDPM ESKVSNIGLR TNQVMEKNKS
260 270 280 290 300
THPVTAHGTA PSTVNPSKQL LVSSSGTQKP KHVVFHNSDF EIIWNKSSMS
310 320 330 340 350
DDDVDSEDEL KMMIAKEENR EKPGHSSVNE SEHDTFEVVR DDFKSNIHKL
360 370 380 390 400
SSSVSLGNNH EYDSSDTDEI IAMKTKNAKV KNSAESSQPE RTVSKKSSFQ
410 420 430 440 450
KIEPSNDCIK VQGINSNKES ALCHGVKFVN PKFPPDSSGS DSEESEEDEE
460 470 480 490 500
YKVLMENCPR VSLTLADLEQ LAGSHRKFPG KDSETNGPQN DSHCKFDTTS
510 520 530 540 550
KNPKTSGDLY NGRQQCILPE EIVASLLEDE NTYSKQKSEE DILKPKFQAF
560 570 580 590 600
KGIGCLYAKE SVDKTLKENI AFNTGGGHHS SLKHEDHNRS LMENGSKCVN
610 620 630 640 650
GSSSKLTSCQ PAKKVNDPNH IQPPKRQCTF ENQNHKVMSS TSCDKGSTNP
660 670 680 690 700
LPGPLPLKAK TSLHLSANSH KVDSDGDACH WPESRKALEK ERTNLSNLES
710 720 730 740 750
LEKSSKVSPR EDPQKSPAGF SLSDSNASCI NAKDKQAEDN QKRLAALAAW
760 770 780 790 800
QKAREVQKKL VHSALANLDG HPEDKKTHIV FASDNESETE ETSTQEQSCP
810 820 830 840 850
EKELMKESVS KSPGKLFDSS DDEDSDSKED STRFSIKPQF EGRAGQKLMD
860 870 880 890 900
LQSQFGSDER FRMDSRFLES DSEDEKKELN EDKVNEDELA AEKKKTLNVV
910 920 930 940 950
QSVLNINVNN PTNKGSVAAK KFKDIVHYDP TKHDHAIYER KQEDKEKESK
960 970 980 990 1000
ATRKKKKEEA EKLPEVSQDM YYNIAADLKE IFQSMSNTDE KEEDVPRTEA
1010 1020 1030 1040 1050
GAREGTGKIR NAETLACEPE QTTGFTFSFF DSATKDEKDA TYRIELVKHG
1060 1070 1080 1090 1100
KIVCPNDPRF QDSSSEEEDI AEEADHSKPS PGEAVPENEA IRFFFFSEND
1110 1120 1130 1140
DRLRGSNLFW SGMGGSISRN SWEARTSSLL LECRKKHKEA KRKVKAN
Length:1,147
Mass (Da):128,635
Last modified:June 13, 2006 - v2
Checksum:i53949DB71021DB38
GO
Isoform 21 Publication (identifier: Q3UHX0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-848: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:299
Mass (Da):34,237
Checksum:iB2DB8BF07F62CF39
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QKD1E9QKD1_MOUSE
Nucleolar protein 8
Nol8
1,165Annotation score:
A0A1Y7VJP6A0A1Y7VJP6_MOUSE
Nucleolar protein 8
Nol8
93Annotation score:
A0A1Y7VN09A0A1Y7VN09_MOUSE
Nucleolar protein 8
Nol8
225Annotation score:
A0A1Y7VNA8A0A1Y7VNA8_MOUSE
Nucleolar protein 8
Nol8
145Annotation score:

Sequence cautioni

The sequence BAB29660 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC26701 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti383S → R in BAC26701 (PubMed:16141072).Curated1
Sequence conflicti482D → G in BAE27736 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0520571 – 848Missing in isoform 2. 1 PublicationAdd BLAST848

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029961 mRNA Translation: BAC26701.1 Different initiation.
AK147172 mRNA Translation: BAE27736.1
AK014994 mRNA Translation: BAB29660.1 Different initiation.
BC094941 mRNA Translation: AAH94941.1
BC125575 mRNA Translation: AAI25576.1
BC125579 mRNA Translation: AAI25580.1
RefSeqiNP_001258326.1, NM_001271397.1
XP_006517019.2, XM_006516956.3 [Q3UHX0-1]
UniGeneiMm.258915

Genome annotation databases

EnsembliENSMUST00000221142; ENSMUSP00000152552; ENSMUSG00000021392 [Q3UHX0-1]
ENSMUST00000223467; ENSMUSP00000152878; ENSMUSG00000021392 [Q3UHX0-1]
GeneIDi70930
KEGGimmu:70930
UCSCiuc033glk.1 mouse [Q3UHX0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029961 mRNA Translation: BAC26701.1 Different initiation.
AK147172 mRNA Translation: BAE27736.1
AK014994 mRNA Translation: BAB29660.1 Different initiation.
BC094941 mRNA Translation: AAH94941.1
BC125575 mRNA Translation: AAI25576.1
BC125579 mRNA Translation: AAI25580.1
RefSeqiNP_001258326.1, NM_001271397.1
XP_006517019.2, XM_006516956.3 [Q3UHX0-1]
UniGeneiMm.258915

3D structure databases

ProteinModelPortaliQ3UHX0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3UHX0, 1 interactor
MINTiQ3UHX0
STRINGi10090.ENSMUSP00000021824

PTM databases

iPTMnetiQ3UHX0
PhosphoSitePlusiQ3UHX0

Proteomic databases

EPDiQ3UHX0
MaxQBiQ3UHX0
PaxDbiQ3UHX0
PeptideAtlasiQ3UHX0
PRIDEiQ3UHX0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000221142; ENSMUSP00000152552; ENSMUSG00000021392 [Q3UHX0-1]
ENSMUST00000223467; ENSMUSP00000152878; ENSMUSG00000021392 [Q3UHX0-1]
GeneIDi70930
KEGGimmu:70930
UCSCiuc033glk.1 mouse [Q3UHX0-1]

Organism-specific databases

CTDi55035
MGIiMGI:1918180 Nol8

Phylogenomic databases

eggNOGiKOG4365 Eukaryota
ENOG410Y5CN LUCA
GeneTreeiENSGT00390000004860
HOGENOMiHOG000293312
HOVERGENiHBG057565
InParanoidiQ3UHX0
OMAiHYDPTRH
PhylomeDBiQ3UHX0

Miscellaneous databases

ChiTaRSiNol8 mouse
PROiPR:Q3UHX0
SOURCEiSearch...

Gene expression databases

CleanExiMM_NOL8
ExpressionAtlasiQ3UHX0 baseline and differential

Family and domain databases

CDDicd12226 RRM_NOL8, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR034138 NOP8_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOL8_MOUSE
AccessioniPrimary (citable) accession number: Q3UHX0
Secondary accession number(s): Q059P5
, Q504M4, Q8CDJ7, Q9CUR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: November 7, 2018
This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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