UniProtKB - Q3UHJ0 (AAK1_MOUSE)
AP2-associated protein kinase 1
Aak1
Functioni
Catalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 74 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 176 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 52 – 60 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- AP-2 adaptor complex binding Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- Notch binding Source: UniProtKB
- protein serine/threonine kinase activity Source: UniProtKB
GO - Biological processi
- endocytosis Source: UniProtKB-KW
- positive regulation of Notch signaling pathway Source: UniProtKB
- protein autophosphorylation Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- protein stabilization Source: UniProtKB
- regulation of clathrin-dependent endocytosis Source: UniProtKB
- regulation of protein localization Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Endocytosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: AP2-associated protein kinase 1 (EC:2.7.11.1By similarity)Alternative name(s): Adaptor-associated kinase 1 |
Gene namesi | Name:Aak1 Synonyms:Kiaa1048 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1098687, Aak1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Other locations
- clathrin-coated pit By similarity
- presynapse By similarity
Note: Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells.By similarity
Plasma Membrane
- clathrin-coated pit Source: UniProtKB-SubCell
- extrinsic component of plasma membrane Source: UniProtKB
Other locations
- cell leading edge Source: UniProtKB
- clathrin complex Source: MGI
- clathrin-coated vesicle Source: UniProtKB
- cytoplasm Source: GO_Central
- presynapse Source: MGI
- terminal bouton Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Coated pit, Membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000250579 | 1 – 959 | AP2-associated protein kinase 1Add BLAST | 959 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 14 | PhosphoserineBy similarity | 1 | |
Modified residuei | 234 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 235 | PhosphoserineBy similarity | 1 | |
Modified residuei | 354 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 389 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 391 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 441 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 604 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 616 | PhosphoserineBy similarity | 1 | |
Modified residuei | 618 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 621 | PhosphoserineBy similarity | 1 | |
Modified residuei | 622 | PhosphoserineCombined sources | 1 | |
Modified residuei | 635 | PhosphoserineCombined sources | 1 | |
Modified residuei | 648 | PhosphoserineBy similarity | 1 | |
Modified residuei | 651 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 729 | PhosphoserineBy similarity | 1 | |
Modified residuei | 844 | PhosphoserineCombined sources | 1 | |
Modified residuei | 935 | PhosphoserineCombined sources | 1 | |
Modified residuei | 936 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | Q3UHJ0 |
jPOSTi | Q3UHJ0 |
MaxQBi | Q3UHJ0 |
PaxDbi | Q3UHJ0 |
PeptideAtlasi | Q3UHJ0 |
PRIDEi | Q3UHJ0 |
PTM databases
iPTMneti | Q3UHJ0 |
PhosphoSitePlusi | Q3UHJ0 |
SwissPalmi | Q3UHJ0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000057230, Expressed in substantia nigra and 303 other tissues |
ExpressionAtlasi | Q3UHJ0, baseline and differential |
Genevisiblei | Q3UHJ0, MM |
Interactioni
Subunit structurei
Interacts (via CBD domain) with clathrin (By similarity).
Interacts with AP-2 complex (By similarity).
Interacts with NUMB (By similarity).
Interacts with alpha-adaptin (By similarity).
Interacts with EPS15 isoform 2 (By similarity).
Interacts with membrane-bound activated NOTCH1 but not with the inactive full-length form of NOTCH1 (PubMed:21464124). Preferentially interacts with monoubiquitinated activated NOTCH1 compared to the non-ubiquitinated form (PubMed:21464124).
By similarity1 PublicationGO - Molecular functioni
- Notch binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 234710, 12 interactors |
IntActi | Q3UHJ0, 4 interactors |
STRINGi | 10090.ENSMUSP00000086948 |
Miscellaneous databases
RNActi | Q3UHJ0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 46 – 315 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 270 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 821 – 958 | Clathrin-binding domain (CBD)By similarityAdd BLAST | 138 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 22 – 25 | Poly-Gly | 4 | |
Compositional biasi | 397 – 612 | Gln-richAdd BLAST | 216 | |
Compositional biasi | 656 – 661 | Poly-Ala | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1989, Eukaryota |
GeneTreei | ENSGT00940000155968 |
HOGENOMi | CLU_000288_109_5_1 |
InParanoidi | Q3UHJ0 |
OMAi | XIQAPIR |
OrthoDBi | 826336at2759 |
PhylomeDBi | Q3UHJ0 |
TreeFami | TF317300 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKKFFDSRRE QGSSGLGSGS SGGGGSSSGL GSGYIGRVFG IGRQQVTVDE
60 70 80 90 100
VLAEGGFALV FLVRTSNGVK CALKRMFVNN EHDLQVCKRE IQIMRDLSGH
110 120 130 140 150
KNIVGYIDSS INNVSSGDVW EVLILMDFCR GGQVVNLMNQ RLQTGFTENE
160 170 180 190 200
VLQIFCDTCE AVARLHQCKT PIIHRDLKVE NILLHDRGHY VLCDFGSATN
210 220 230 240 250
KFQNPQAEGV NAVEDEIKKY TTLSYRAPEM VNLYSGKIIT TKADIWALGC
260 270 280 290 300
LLYKLCYFTL PFGESQVAIC DGSFTIPDNS RYSQDMHCLI RYMLEPDPDK
310 320 330 340 350
RPDIYQVSYF SFKLLKKECP VPNVQNSPIP AKLPEPVKAS EAAVKKTQPK
360 370 380 390 400
ARLTDPIPTT ETSIAPRQRP KAGQTQPNPG ILPIQPALTP RKRATVQPLP
410 420 430 440 450
QAAGPSNQPG LLPSVSQPKA QATPSQPLQS SQPKQPQAPP TPQQTPATQT
460 470 480 490 500
QGLPTQAQAT PQHQQQHLLK QQQQQQQQPQ QPTAPPQPAG TFYQQQQQQQ
510 520 530 540 550
QQQAQTQQFQ AVHPAAQQPV TAQFPVGSQG GAQQQLMQNF YHQQQQQQQQ
560 570 580 590 600
QQQLMAQQAA LQQKTAVVVP QSQAQPATAP QAAAAQEPGQ IQAPVRQQPK
610 620 630 640 650
VQTTPPPTIQ GQKVGSLTPP SSPKTQRAGH RRILSDVTHS AVFGVPASKS
660 670 680 690 700
TQLLQAAAAE ASLNKSKSAT TTPSGSPRTS QQNVSNASEG STWNPFDDDN
710 720 730 740 750
FSKLTAEELL NKDFAKLGEG KLPEKLGGSA ESLIPGFQPT QGDAFTTPSF
760 770 780 790 800
SAGTAEKRKG GQAVDSGIPL LSVSDPFIPL QVPDAPEKLI EGLKSPDTSL
810 820 830 840 850
LLPDLLPMTD PFGSTSDAVI DKADVAVESL IPGLEPPVAQ RLPSQTESVT
860 870 880 890 900
SNRTDSLTGE DSLLDCSLLS NPTAGLLEEF APIALSAPTH KAAEDSNLIS
910 920 930 940 950
GFGVAEGSEK VAEDEFDPIP VLITKNTQGG HSRNSSGSSE SSLPNLARSL
LLVDQLIDL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0N4SVA1 | A0A0N4SVA1_MOUSE | AP2-associated protein kinase 1 | Aak1 | 121 | Annotation score: | ||
A0A571BEI2 | A0A571BEI2_MOUSE | AP2-associated protein kinase 1 | Aak1 | 510 | Annotation score: | ||
A0A571BDM4 | A0A571BDM4_MOUSE | AP2-associated protein kinase 1 | Aak1 | 237 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 79 | N → K in BAE27867 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 686 | N → S in BAC98082 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 733 | L → V in AAI41177 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020670 | 509 – 589 | Missing in isoform 2. 1 PublicationAdd BLAST | 81 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK147363 mRNA Translation: BAE27867.1 AK158879 mRNA Translation: BAE34710.1 BC043125 mRNA Translation: AAH43125.1 BC141176 mRNA Translation: AAI41177.1 AK129272 mRNA Translation: BAC98082.1 |
CCDSi | CCDS20317.1 [Q3UHJ0-2] CCDS39544.1 [Q3UHJ0-1] |
RefSeqi | NP_001035195.1, NM_001040106.2 [Q3UHJ0-1] NP_808430.2, NM_177762.6 [Q3UHJ0-2] |
Genome annotation databases
Ensembli | ENSMUST00000003710; ENSMUSP00000003710; ENSMUSG00000057230 [Q3UHJ0-2] ENSMUST00000089519; ENSMUSP00000086948; ENSMUSG00000057230 [Q3UHJ0-1] |
GeneIDi | 269774 |
KEGGi | mmu:269774 |
UCSCi | uc009css.1, mouse [Q3UHJ0-1] uc009cst.1, mouse [Q3UHJ0-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK147363 mRNA Translation: BAE27867.1 AK158879 mRNA Translation: BAE34710.1 BC043125 mRNA Translation: AAH43125.1 BC141176 mRNA Translation: AAI41177.1 AK129272 mRNA Translation: BAC98082.1 |
CCDSi | CCDS20317.1 [Q3UHJ0-2] CCDS39544.1 [Q3UHJ0-1] |
RefSeqi | NP_001035195.1, NM_001040106.2 [Q3UHJ0-1] NP_808430.2, NM_177762.6 [Q3UHJ0-2] |
3D structure databases
SMRi | Q3UHJ0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 234710, 12 interactors |
IntActi | Q3UHJ0, 4 interactors |
STRINGi | 10090.ENSMUSP00000086948 |
PTM databases
iPTMneti | Q3UHJ0 |
PhosphoSitePlusi | Q3UHJ0 |
SwissPalmi | Q3UHJ0 |
Proteomic databases
EPDi | Q3UHJ0 |
jPOSTi | Q3UHJ0 |
MaxQBi | Q3UHJ0 |
PaxDbi | Q3UHJ0 |
PeptideAtlasi | Q3UHJ0 |
PRIDEi | Q3UHJ0 |
Protocols and materials databases
Antibodypediai | 7943, 344 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000003710; ENSMUSP00000003710; ENSMUSG00000057230 [Q3UHJ0-2] ENSMUST00000089519; ENSMUSP00000086948; ENSMUSG00000057230 [Q3UHJ0-1] |
GeneIDi | 269774 |
KEGGi | mmu:269774 |
UCSCi | uc009css.1, mouse [Q3UHJ0-1] uc009cst.1, mouse [Q3UHJ0-2] |
Organism-specific databases
CTDi | 22848 |
MGIi | MGI:1098687, Aak1 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1989, Eukaryota |
GeneTreei | ENSGT00940000155968 |
HOGENOMi | CLU_000288_109_5_1 |
InParanoidi | Q3UHJ0 |
OMAi | XIQAPIR |
OrthoDBi | 826336at2759 |
PhylomeDBi | Q3UHJ0 |
TreeFami | TF317300 |
Enzyme and pathway databases
Reactomei | R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
BioGRID-ORCSi | 269774, 0 hits in 17 CRISPR screens |
ChiTaRSi | Aak1, mouse |
PROi | PR:Q3UHJ0 |
RNActi | Q3UHJ0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000057230, Expressed in substantia nigra and 303 other tissues |
ExpressionAtlasi | Q3UHJ0, baseline and differential |
Genevisiblei | Q3UHJ0, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AAK1_MOUSE | |
Accessioni | Q3UHJ0Primary (citable) accession number: Q3UHJ0 Secondary accession number(s): B2RUJ0 Q80XP6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2006 |
Last sequence update: | October 3, 2006 | |
Last modified: | December 2, 2020 | |
This is version 133 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index