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Protein

Sorting nexin-27

Gene

Snx27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-27
Gene namesi
Name:Snx27
Synonyms:Kiaa0488
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1923992 Snx27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Growth retardation followed by lethality. Some mice die in the uterus during embryonic development. Newborn mice that survive fail to thrive and all die at different times within the first 3 weeks. The body weight of newborn is lower than wild-type mice, and the postnatal growth is severely retarded, with a clear retardation of body weight gain. The growth retardation is not only reflected in the body weight, but also in multiple organs, such as the spleen, kidney, liver, heart and intestine. Mice also show neuronal deficits in the hippocampus and cortex: despite a normal neuroanatomy, defects in synaptic function, learning and memory and a reduction in the amounts of ionotropic glutamate receptors (NMDA and AMPA receptors) are observed.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003153571 – 539Sorting nexin-27Add BLAST539

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphoserineCombined sources1
Modified residuei60PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UHD6
PaxDbiQ3UHD6
PeptideAtlasiQ3UHD6
PRIDEiQ3UHD6

PTM databases

iPTMnetiQ3UHD6
PhosphoSitePlusiQ3UHD6

Expressioni

Tissue specificityi

Expressed in cells of hematopoietic origin.1 Publication

Gene expression databases

BgeeiENSMUSG00000028136
CleanExiMM_SNX27
ExpressionAtlasiQ3UHD6 baseline and differential
GenevisibleiQ3UHD6 MM

Interactioni

Subunit structurei

Core component of the SNX27-retromer, a multiprotein complex composed of SNX27, the WASH complex and the retromer complex. Interacts (via the FERM-like regions) with the WASH complex. Interacts with SNX1. Interacts with CYTIP. Interacts with DGKZ. Interacts with MCC (By similarity). Interacts (via PDZ domain) with a number of target transmembrane proteins (via PDZ-binding motif): ABCC4, ADRB2, ARHGEF7, GRIA1, GRIA2, GRIN1, GRIN2A GRIN2C, KCNJ6, KCNJ9 and SLC2A1/GLUT1.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi218291, 2 interactors
DIPiDIP-60337N
ELMiQ3UHD6
IntActiQ3UHD6, 3 interactors
STRINGi10090.ENSMUSP00000102904

Structurei

3D structure databases

ProteinModelPortaliQ3UHD6
SMRiQ3UHD6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 134PDZPROSITE-ProRule annotationAdd BLAST94
Domaini159 – 267PXPROSITE-ProRule annotationAdd BLAST109
Domaini271 – 360Ras-associatingPROSITE-ProRule annotationAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni271 – 360FERM-like region F1By similarityAdd BLAST90
Regioni371 – 419FERM-like region F2By similarityAdd BLAST49
Regioni423 – 523FERM-like region F3By similarityAdd BLAST101

Domaini

The PDZ domain mediates binding to a subset of proteins containing a PDZ-binding motif at the C-terminus: the specificity for PDZ-binding motif is provided by the 2 residues located upstream of the canonical PDZ-binding motif. The PDZ domain also mediates binding to the retromer complex via direct interaction with VPS26 (VPS26A or VPS26B).By similarity
The PX domain mediates binding to phosphatidylinositol 3-phosphate (PtdIns(3P)) and localization to early endosome membranes.By similarity

Phylogenomic databases

eggNOGiENOG410IMRD Eukaryota
ENOG410XPAD LUCA
GeneTreeiENSGT00530000063147
HOGENOMiHOG000016641
HOVERGENiHBG059206
InParanoidiQ3UHD6
KOiK17936
OMAiIFPHCSC
OrthoDBiEOG091G147N
PhylomeDBiQ3UHD6
TreeFamiTF318398

Family and domain databases

CDDicd13338 FERM-like_C_SNX27, 1 hit
cd06886 PX_SNX27, 1 hit
cd01777 SNX27_RA, 1 hit
Gene3Di3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR000159 RA_dom
IPR037831 SNX17/27/31
IPR028667 SNX27
IPR037827 SNX27_FERM-like_dom
IPR037833 SNX27_PX
IPR037835 SNX27_RA
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR12431 PTHR12431, 1 hit
PTHR12431:SF17 PTHR12431:SF17, 1 hit
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
PF00787 PX, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00312 PX, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
SSF54236 SSF54236, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit
PS50195 PX, 1 hit
PS50200 RA, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UHD6-1) [UniParc]FASTAAdd to basket
Also known as: SNX27a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADEDGEGIH PSAPHRNGGG GGGSGLHCAG NGGGGGGGPR VVRIVKSESG
60 70 80 90 100
YGFNVRGQVS EGGQLRSING ELYAPLQHVS AVLPGGAADR AGVRKGDRIL
110 120 130 140 150
EVNGVNVEGA THKQVVDLIR AGEKELILTV LSVPPHEADN LDPSDDSLGQ
160 170 180 190 200
SFYDYTEKQA VPISVPTYKH VEQNGEKFVV YNVYMAGRQL CSKRYREFAI
210 220 230 240 250
LHQNLKREFA NFTFPRLPGK WPFSLSEQQL DARRRGLEEY LEKVCSIRVI
260 270 280 290 300
GESDIMQEFL SESDENYNGV SDVELRVALP DGTTVTVRVK KNSTTDQVYQ
310 320 330 340 350
AIAAKVGMDS TTVNYFALFE VINHSFVRKL APNEFPHKLY VQNYTSAVPG
360 370 380 390 400
TCLTIRKWLF TTEEEVLLND NDLAVTYFFH QAVDDVKKGY IKAEEKSYQL
410 420 430 440 450
QKLHEQRKMV MYLNMLRTCE GYNEIIFPHC ACDSRRKGHV ITAISITHFK
460 470 480 490 500
LHACTEEGQL ENQVIAFEWD EMQRWDTDEE GMAFCFEYAR GEKKPRWVKI
510 520 530
FTPYFNYMHE CFERVFCELK WRKENIFQMA RSQQRDVAT
Length:539
Mass (Da):60,989
Last modified:January 15, 2008 - v2
Checksum:i0EF5B84253A90B4D
GO
Isoform 2 (identifier: Q3UHD6-2) [UniParc]FASTAAdd to basket
Also known as: SNX27b

The sequence of this isoform differs from the canonical sequence as follows:
     525-539: NIFQMARSQQRDVAT → EY

Show »
Length:526
Mass (Da):59,534
Checksum:i4623CCCAC326F5AC
GO

Sequence cautioni

The sequence BAB30966 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB30966 differs from that shown. Reason: Erroneous termination at position 258. Translated as Glu.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030540525 – 539NIFQM…RDVAT → EY in isoform 2. 2 PublicationsAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017836 mRNA Translation: BAB30966.1 Sequence problems.
AK147452 mRNA Translation: BAE27921.1
AK122296 mRNA Translation: BAC65578.1
BC053495 mRNA Translation: AAH53495.1
CCDSiCCDS38537.1 [Q3UHD6-1]
CCDS38538.1 [Q3UHD6-2]
RefSeqiNP_001075953.1, NM_001082484.2 [Q3UHD6-1]
NP_083997.1, NM_029721.2 [Q3UHD6-2]
UniGeneiMm.28160

Genome annotation databases

EnsembliENSMUST00000029783; ENSMUSP00000029783; ENSMUSG00000028136 [Q3UHD6-2]
ENSMUST00000107283; ENSMUSP00000102904; ENSMUSG00000028136 [Q3UHD6-1]
GeneIDi76742
KEGGimmu:76742
UCSCiuc008qgr.2 mouse [Q3UHD6-2]
uc008qgs.2 mouse [Q3UHD6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSNX27_MOUSE
AccessioniPrimary (citable) accession number: Q3UHD6
Secondary accession number(s): Q7TQL6, Q80TZ1, Q9CYB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: June 20, 2018
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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