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Entry version 117 (16 Oct 2019)
Sequence version 2 (20 Feb 2007)
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Protein

Serine/threonine-protein kinase WNK2

Gene

Wnk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.1 Publication

Caution

Was named WNK/'with no lysine(K)' because key residues for catalysis, including the lysine involved in ATP binding, are either not conserved or differ compared to the residues described in other kinase family proteins.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activation requires autophosphorylation of Ser-356. Phosphorylation of Ser-352 also promotes increased activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei205ATP; via amide nitrogenBy similarity1
Binding sitei325ATPBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei342Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi275 – 278ATPBy similarity4

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-2672351 Stimuli-sensing channels

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Serine/threonine-protein kinase WNK2Curated (EC:2.7.11.1By similarity)
Alternative name(s):
Protein kinase lysine-deficient 2Imported
Protein kinase with no lysine 21 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Wnk2Imported
Synonyms:Kiaa1760Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unplaced

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:1922857 Wnk2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002787741 – 2149Serine/threonine-protein kinase WNK2Add BLAST2149

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei19Omega-N-methylarginineCombined sources1
Modified residuei30Omega-N-methylarginineCombined sources1
Modified residuei45PhosphoserineBy similarity1
Modified residuei352Phosphoserine; by autocatalysisBy similarity1
Modified residuei356Phosphoserine; by autocatalysisBy similarity1
Modified residuei560PhosphoserineBy similarity1
Modified residuei1098PhosphoserineBy similarity1
Modified residuei1210PhosphoserineBy similarity1
Modified residuei1507PhosphoserineBy similarity1
Modified residuei1566Phosphoserine1 Publication1
Modified residuei1594PhosphoserineBy similarity1
Modified residuei1725PhosphoserineCombined sources1
Modified residuei1726PhosphoserineCombined sources1
Modified residuei1770Phosphoserine1 Publication1
Modified residuei1797Phosphoserine1 Publication1
Modified residuei1962PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
Q3UH66

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q3UH66

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q3UH66

PeptideAtlas

More...
PeptideAtlasi
Q3UH66

PRoteomics IDEntifications database

More...
PRIDEi
Q3UH66

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q3UH66

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q3UH66

SwissPalm database of S-palmitoylation events

More...
SwissPalmi
Q3UH66

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Brain and heart.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Forms a complex with the phosphorylated form of STK39 in the brain.

1 Publication

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
217612, 1 interactor

Protein interaction database and analysis system

More...
IntActi
Q3UH66, 1 interactor

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000089212

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q3UH66

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini195 – 453Protein kinasePROSITE-ProRule annotationAdd BLAST259

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.PROSITE-ProRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0584 Eukaryota
ENOG410XQWZ LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000139922

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q3UH66

KEGG Orthology (KO)

More...
KOi
K08867

Database of Orthologous Groups

More...
OrthoDBi
27514at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q3UH66

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR024678 Kinase_OSR1/WNK_CCT
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF12202 OSR1_C, 1 hit
PF00069 Pkinase, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00220 S_TKc, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56112 SSF56112, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (7+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 7 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 7 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q3UH66-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDGDGGRRDA PGALMEAGRG TGSAGMAEPR ARAARLGPQR FLRRSVVESD
60 70 80 90 100
QEEPPGLEAA ETPSAQPPQP LQRRVLLLCK TRRLIAERAR GRPAAPAPAA
110 120 130 140 150
PAAPPGSPSV PSDPGPERAG TQEPSPDPTT ASAAATQVPD GGPRQEEAPA
160 170 180 190 200
PTQEDAGTTE AKPEPGRARK DEPEEEEDDE DDLKAVATSL DGRFLKFDIE
210 220 230 240 250
LGRGSFKTVY KGLDTETWVE VAWCELQDRK LTKLERQRFK EEAEMLKGLQ
260 270 280 290 300
HPNIVRFYDF WESSAKGKRC IVLVTELMTS GTLKTYLKRF KVMKPKVLRS
310 320 330 340 350
WCRQILKGLL FLHTRTPPII HRDLKCDNIF ITGPTGSVKI GDLGLATLKR
360 370 380 390 400
ASFAKSVIGT PEFMAPEMYE EHYDESVDVY AFGMCMLEMA TSEYPYSECQ
410 420 430 440 450
NAAQIYRKVT CGIKPASFEK VHDPEIKEII GECICKNKEE RYEIKDLLSH
460 470 480 490 500
AFFAEDTGVR VELAEEDHGR KSTIALRLWV EDPKKLKGKP KDNGAIEFTF
510 520 530 540 550
DLEKETPDEV AQEMIDSGFF HESDVKIVAK SIRDRVALIQ WRRERIWPAL
560 570 580 590 600
QSQEPKDSGS PDKARGLPAP LQVQVTYHAQ SGQPGQPEPE EPEADQHLLP
610 620 630 640 650
PTLPASVTSL ASDSTFDSGQ GSTVYSDSQS SQQSMVLSSL VDTAPTPASC
660 670 680 690 700
VCSPPVSEGP GLTHSLPTLG AFQQPATVPG LSVGPVPPPA RPPLLQQHFP
710 720 730 740 750
ESSMSFTPVL PPPSTPVPTG PSQPAPPVQQ PLPMAQPPTL PQVLAPQPMG
760 770 780 790 800
TVQPVPSHLP PYLAPTSQVV APAQLKPLQM PQPPLQPLAQ VPPQMPQMPV
810 820 830 840 850
VPPITPLTGL DGLPQTLTDL PAANVAPVPP PQYFSPAVIL PSLTTPLPTS
860 870 880 890 900
PALPMQAVKL PHPPGTPLAV PCQTIVPNAP AAIPLLAVAP QGVAALSIHP
910 920 930 940 950
AVAQIPAQPV YPAAFPQMVP GDIPPSPHHT VQSLRATPPQ LASPVPPQPV
960 970 980 990 1000
QPSVIHLPEQ AAPTAASGTQ VLLGHPPSYT ADVAAPVSAV SLPPAVLSPP
1010 1020 1030 1040 1050
LPDTLLPTVP DLLPKVPSSL APTVVAASQS APAQTSSLLL PTNPPLPTGP
1060 1070 1080 1090 1100
AVAGPCPAVQ LMVEVAQEEQ VSQDKPPGPP QSSESFGGSD VTSGRDLSDS
1110 1120 1130 1140 1150
CEGTFGGGRL EGRTARKHHR RSTRARSRQE RASRPRLTIL NVCNTGDKMV
1160 1170 1180 1190 1200
ECQLETHNHK MVTFKFDLDG DAPDEIATYM VEHDFILPAE RETFIEQMKD
1210 1220 1230 1240 1250
VMDKAEDMLS EDTDADHGSD TGTSPPHLGT CGLATGEENR QSQANAPVYQ
1260 1270 1280 1290 1300
QNVLHTGKRW FIICPVAEHP ATDTSESSPP LPLSSLQPEA SQDPAPYPDQ
1310 1320 1330 1340 1350
LSLTDKPSFP AAQQLLSQAG SSNPPGGASA PLAPSSPPVT TVIPAAPATS
1360 1370 1380 1390 1400
TVPESAAGTA MQAGGPGTHQ GPASVHETLQ PLAETRSAQC TAQPLSTGQG
1410 1420 1430 1440 1450
PCTPALEASR CSTGLGEPIS TREVSTQGEP LPASVPEPSP PTGATQSVPG
1460 1470 1480 1490 1500
QPPPPLPITV GAISLAAPQL PSPPLGPTAP PPPPSALESD GEGPPPRVGF
1510 1520 1530 1540 1550
VDNTIKSLDE KLRTLLYQEH VPTSSASAGT PMEASDRDFT LEPLRGDLPS
1560 1570 1580 1590 1600
ALSDKTPSLT QQTQPSLEKS ETAPAGWALA QREQGASSPM TAESSSSNTL
1610 1620 1630 1640 1650
GCDSDAGQVA SDSSTAPSVP QDASGSSVPT HMDPKDQNSS VPREALAAPM
1660 1670 1680 1690 1700
QSGPGSFTVG SPAQLRGARD SGSPHKRPGQ QDNSSPAKTV GRFSVVSTQD
1710 1720 1730 1740 1750
EWTLASPHSL RYSAPPDVYL DEIPSSPEVK LAVRRVQTAS SIEVGVEEPA
1760 1770 1780 1790 1800
SSDSGDERPR RRSQVQKQSS LPGTGGVASD FVKKATAFLH RSSRAGSLGP
1810 1820 1830 1840 1850
ETPSRAGVKV PTISITSFHS QSSYISSDND SEFEDADIKK ELRSLREKHL
1860 1870 1880 1890 1900
KEISELQSQQ KQEIEALYRR LGKPLPPNVG FFHTAPPMGR RRKTSKSKLK
1910 1920 1930 1940 1950
AGKLLNPLVQ QLKVVASSTG HLSDSSRGPP TKDPRGTKAV QTQQPCSVRA
1960 1970 1980 1990 2000
SLSTDICSGL ASDGGGARGQ GWTVYHPTSE RGAYKSSSKP RARFLSGPVS
2010 2020 2030 2040 2050
VSIWSALKRL CLGKEHSSSL YDSPGSSTSS LAPGPEPGPQ PTLHVQAQVN
2060 2070 2080 2090 2100
NSNNKKGTFT DDLHKLVDEW TTKTVGAAQV KPTLNQLKQT QKLHDMEASG
2110 2120 2130 2140
DARATSVPRA AVGASCLAPA PGPLSTTATP GATPALPVPI PDPESEKPD
Note: No experimental confirmation available.
Length:2,149
Mass (Da):227,527
Last modified:February 20, 2007 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA4BBF1D391FE9BEC
GO
Isoform 2 (identifier: Q3UH66-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1921-2004: Missing.

Note: No experimental confirmation available.
Show »
Length:2,065
Mass (Da):218,728
Checksum:iCF4AE4A4DC664F90
GO
Isoform 3 (identifier: Q3UH66-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1935-1935: R → AHASTPP
     2019-2025: SLYDSPG → R

Note: No experimental confirmation available.
Show »
Length:2,149
Mass (Da):227,469
Checksum:i98AD382CFD4CFE45
GO
Isoform 4 (identifier: Q3UH66-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1935-1935: R → AHASTPP
     1971-2024: Missing.

Note: No experimental confirmation available.
Show »
Length:2,101
Mass (Da):222,082
Checksum:i953172919B0CBF8D
GO
Isoform 5 (identifier: Q3UH66-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1935-1935: R → AHASTPP
     2019-2025: SLYDSPG → R
     2142-2149: DPESEKPD → GTVHSSLSGP...STPGPRLHIT

Note: No experimental confirmation available.
Show »
Length:2,213
Mass (Da):234,069
Checksum:i0BE7B0D8B10819E2
GO
Isoform 6 (identifier: Q3UH66-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     731-742: Missing.
     971-1068: Missing.
     1935-1935: R → AHASTPP
     1971-2024: Missing.

Note: No experimental confirmation available.
Show »
Length:1,991
Mass (Da):211,147
Checksum:i1177FFA99C62BFD0
GO
Isoform 7 (identifier: Q3UH66-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     971-1068: Missing.
     1592-1593: AE → E
     1935-1935: R → AHASTPP
     2019-2025: SLYDSPG → R
     2142-2149: DPESEKPD → EACALPTPPCKFLSRPSSGQPTEGRSISGGFHDTCPGGERGDENSLTPSG

Note: No experimental confirmation available.
Show »
Length:2,092
Mass (Da):221,908
Checksum:i581DA7240F2E5FE5
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QM73E9QM73_MOUSE
Serine/threonine-protein kinase WNK...
Wnk2
2,213Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9QMI8E9QMI8_MOUSE
Serine/threonine-protein kinase WNK...
Wnk2
2,149Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9QMI9E9QMI9_MOUSE
Serine/threonine-protein kinase WNK...
Wnk2
2,092Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E0CYT1E0CYT1_MOUSE
Serine/threonine-protein kinase WNK...
Wnk2
2,137Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9Q6Q2E9Q6Q2_MOUSE
Serine/threonine-protein kinase WNK...
Wnk2
1,991Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9QJQ9E9QJQ9_MOUSE
Serine/threonine-protein kinase WNK...
Wnk2
2,101Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E0CZE3E0CZE3_MOUSE
Serine/threonine-protein kinase WNK...
Wnk2
2,065Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F6SRP4F6SRP4_MOUSE
Serine/threonine-protein kinase WNK...
Wnk2
708Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0R4J1Z1A0A0R4J1Z1_MOUSE
Serine/threonine-protein kinase WNK...
Wnk2
1,332Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F7BQG5F7BQG5_MOUSE
Serine/threonine-protein kinase WNK...
Wnk2
138Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAH46464 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAH55795 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAE20646 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti761 – 794PYLAP…QVPPQ → RRYLWPIFCSPQGWGTRPWT RPVHACLQGLLARE in BAC98249 (PubMed:14621295).CuratedAdd BLAST34

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_023366731 – 742Missing in isoform 6. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_023367971 – 1068Missing in isoform 6 and isoform 7. 2 PublicationsAdd BLAST98
Alternative sequenceiVSP_0233681592 – 1593AE → E in isoform 7. 1 Publication2
Alternative sequenceiVSP_0233691921 – 2004Missing in isoform 2. 1 PublicationAdd BLAST84
Alternative sequenceiVSP_0233701935R → AHASTPP in isoform 3, isoform 4, isoform 5, isoform 6 and isoform 7. 3 Publications1
Alternative sequenceiVSP_0233711971 – 2024Missing in isoform 4 and isoform 6. 1 PublicationAdd BLAST54
Alternative sequenceiVSP_0233722019 – 2025SLYDSPG → R in isoform 3, isoform 5 and isoform 7. 3 Publications7
Alternative sequenceiVSP_0233732142 – 2149DPESEKPD → GTVHSSLSGPPCTLPLCQYG GLLPDPVSWGPWVASGNPEG SWGSQSPTQVPVFPVFLRPP VISTPGPRLHIT in isoform 5. 1 Publication8
Alternative sequenceiVSP_0233742142 – 2149DPESEKPD → EACALPTPPCKFLSRPSSGQ PTEGRSISGGFHDTCPGGER GDENSLTPSG in isoform 7. 1 Publication8

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
CAAA01187366 Genomic DNA No translation available.
AC140284 Genomic DNA No translation available.
AK044381 mRNA Translation: BAE20646.1 Different initiation.
AK147550 mRNA Translation: BAE27991.1
BC046464 mRNA Translation: AAH46464.1 Different initiation.
BC055795 mRNA Translation: AAH55795.1 Different initiation.
BC060187 mRNA Translation: AAH60187.1
AK129439 mRNA Translation: BAC98249.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS70454.1 [Q3UH66-5]
CCDS70455.1 [Q3UH66-3]

NCBI Reference Sequences

More...
RefSeqi
NP_001277240.1, NM_001290311.1
NP_001277242.1, NM_001290313.1
NP_083637.2, NM_029361.4

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
75607

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:75607

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CAAA01187366 Genomic DNA No translation available.
AC140284 Genomic DNA No translation available.
AK044381 mRNA Translation: BAE20646.1 Different initiation.
AK147550 mRNA Translation: BAE27991.1
BC046464 mRNA Translation: AAH46464.1 Different initiation.
BC055795 mRNA Translation: AAH55795.1 Different initiation.
BC060187 mRNA Translation: AAH60187.1
AK129439 mRNA Translation: BAC98249.1
CCDSiCCDS70454.1 [Q3UH66-5]
CCDS70455.1 [Q3UH66-3]
RefSeqiNP_001277240.1, NM_001290311.1
NP_001277242.1, NM_001290313.1
NP_083637.2, NM_029361.4

3D structure databases

SMRiQ3UH66
ModBaseiSearch...

Protein-protein interaction databases

BioGridi217612, 1 interactor
IntActiQ3UH66, 1 interactor
STRINGi10090.ENSMUSP00000089212

PTM databases

iPTMnetiQ3UH66
PhosphoSitePlusiQ3UH66
SwissPalmiQ3UH66

Proteomic databases

jPOSTiQ3UH66
MaxQBiQ3UH66
PaxDbiQ3UH66
PeptideAtlasiQ3UH66
PRIDEiQ3UH66

Genome annotation databases

GeneIDi75607
KEGGimmu:75607

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
65268
MGIiMGI:1922857 Wnk2

Rodent Unidentified Gene-Encoded large proteins database

More...
Rougei
Search...

Phylogenomic databases

eggNOGiKOG0584 Eukaryota
ENOG410XQWZ LUCA
HOGENOMiHOG000139922
InParanoidiQ3UH66
KOiK08867
OrthoDBi27514at2759
PhylomeDBiQ3UH66

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Wnk2 mouse

Protein Ontology

More...
PROi
PR:Q3UH66

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR024678 Kinase_OSR1/WNK_CCT
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF12202 OSR1_C, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiWNK2_MOUSE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q3UH66
Secondary accession number(s): Q3V387
, Q6PAN9, Q6ZPI6, Q7TNS1, Q811F2
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: October 16, 2019
This is version 117 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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