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Protein

Protein sidekick-1

Gene

Sdk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adhesion molecule that promotes lamina-specific synaptic connections in the retina. Expressed in specific subsets of interneurons and retinal ganglion cells (RGCs) and promotes synaptic connectivity via homophilic interactions.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-373756 SDK interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Protein sidekick-11 Publication
Gene namesi
Name:Sdk11 PublicationImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2444413 Sdk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini? – 1991ExtracellularSequence analysis
Transmembranei1992 – 2012HelicalSequence analysisAdd BLAST21
Topological domaini2013 – 2193CytoplasmicSequence analysisAdd BLAST181

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi227 – 232Missing : Abolishes homophilic interactions. 1 Publication6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000226976? – 2193Protein sidekick-1
Signal peptidei1 – ?Sequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi108 ↔ 151PROSITE-ProRule annotation
Glycosylationi123N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi253N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi283N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi297 ↔ 344PROSITE-ProRule annotation
Disulfide bondi390 ↔ 440PROSITE-ProRule annotation
Disulfide bondi483 ↔ 535PROSITE-ProRule annotation
Glycosylationi532N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi545N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi554N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi577 ↔ 629PROSITE-ProRule annotation
Glycosylationi764N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi803N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi864N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi997N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1006N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1264N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1315N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1636N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1730N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1801N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1875N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ3UH53
PeptideAtlasiQ3UH53
PRIDEiQ3UH53

PTM databases

iPTMnetiQ3UH53
PhosphoSitePlusiQ3UH53

Expressioni

Tissue specificityi

Expressed by non-overlapping subsets of retinal neurons. Sdk1 and Sdk2 are expressed in non-overlapping subsets of interneurons and retinal ganglion cells (RGCs) that form synapses in distinct inner plexiform layer (IPL) sublaminae (at protein level).1 Publication

Developmental stagei

Highly expressed in many fetal tissues, inlcuding kidney but shows markedly lower expression in adult organs. Expression in kidney is high throughout development with maximal expression occurring near birth.1 Publication

Gene expression databases

BgeeiENSMUSG00000039683 Expressed in 124 organ(s), highest expression level in embryo
CleanExiMM_SDK1
GenevisibleiQ3UH53 MM

Interactioni

Subunit structurei

Homodimer; mediates homophilic interactions to promote cell adhesion (PubMed:15703275). Interacts (via PDZ-binding motif) with MAGI1, MAGI2, DLG2, DLG3 and DLG4 (PubMed:20219992). Isoform 2: Does not mediate homophilic interactions (PubMed:15703275).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082928

Structurei

Secondary structure

12193
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ3UH53
SMRiQ3UH53
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 168Ig-like C2-type 1Add BLAST83
Domaini173 – 259Ig-like C2-type 2Add BLAST87
Domaini275 – 363Ig-like C2-type 3Add BLAST89
Domaini368 – 458Ig-like C2-type 4Add BLAST91
Domaini462 – 551Ig-like C2-type 5Add BLAST90
Domaini556 – 645Ig-like C2-type 6Add BLAST90
Domaini652 – 748Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST97
Domaini753 – 849Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST97
Domaini854 – 952Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST99
Domaini956 – 1050Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST95
Domaini1054 – 1153Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST100
Domaini1158 – 1256Fibronectin type-III 6PROSITE-ProRule annotationAdd BLAST99
Domaini1261 – 1358Fibronectin type-III 7PROSITE-ProRule annotationAdd BLAST98
Domaini1362 – 1456Fibronectin type-III 8PROSITE-ProRule annotationAdd BLAST95
Domaini1461 – 1558Fibronectin type-III 9PROSITE-ProRule annotationAdd BLAST98
Domaini1563 – 1681Fibronectin type-III 10PROSITE-ProRule annotationAdd BLAST119
Domaini1686 – 1782Fibronectin type-III 11PROSITE-ProRule annotationAdd BLAST97
Domaini1786 – 1881Fibronectin type-III 12PROSITE-ProRule annotationAdd BLAST96
Domaini1884 – 1982Fibronectin type-III 13PROSITE-ProRule annotationAdd BLAST99

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2187 – 2193PDZ-bindingBy similarity7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi68 – 76Poly-Leu9

Domaini

the PDZ-binding motif mediates interaction with PDZ domain-containing proteins MAGI1, MAGI2, DLG2, DLG3 and DLG4 and is required for is required for synaptic localization in photoreceptors.By similarity
Ig-like C2-type domains 1 and 2 mediate homophilic interactions.1 Publication

Sequence similaritiesi

Belongs to the sidekick family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3510 Eukaryota
ENOG410ZHW1 LUCA
GeneTreeiENSGT00910000144009
HOGENOMiHOG000231052
HOVERGENiHBG080083
InParanoidiQ3UH53
KOiK16353
OMAiPETEHNG
OrthoDBiEOG091G0607
PhylomeDBiQ3UH53
TreeFamiTF316846

Family and domain databases

CDDicd00063 FN3, 13 hits
Gene3Di2.60.40.10, 19 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
PfamiView protein in Pfam
PF00041 fn3, 13 hits
PF07679 I-set, 4 hits
SMARTiView protein in SMART
SM00060 FN3, 13 hits
SM00409 IG, 6 hits
SM00408 IGc2, 5 hits
SUPFAMiSSF48726 SSF48726, 6 hits
SSF49265 SSF49265, 7 hits
PROSITEiView protein in PROSITE
PS50853 FN3, 13 hits
PS50835 IG_LIKE, 5 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q3UH53-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MARARPSVAG GGVAAPPERA GPGRPRRSRT GHHCDPECPG LRAAPRTPGP
60 70 80 90 100
GAGRRAAKLR PGRGWWALLL LQLHLLRALA QDDVAPYFKT EPGLPQIHLE
110 120 130 140 150
GNRLVLTCLA EGSWPLEFKW IRNDSELTTY SSEYKYIIPS LQKLDAGFYR
160 170 180 190 200
CVVRNRMGAL LQRKSEIQVA YMGNFMDTDQ RKTVSQGHAA LLNLLPIVSC
210 220 230 240 250
PQPQVTWFRE GHKIIPSSRI AITLENQLVI LATTASDAGA YYVQAVNEKN
260 270 280 290 300
GENKTSPFIH LSVARDTGTH EAMAPIIVVA PGNRSVVAGS SETTLECIAN
310 320 330 340 350
ARPVEELSVH WKRNGVRLTS GLHSYGRRLT ITNPTSADTG MYVCEATLRG
360 370 380 390 400
STFEPARARA FLSIIEPPYF TAEPESRILG EVEETMDIPC RAMGVPLPTL
410 420 430 440 450
QWYKDAVPLS KLQNPRYKVL PSGGLHIQKL SPEDSGIFQC FASNEGGEVQ
460 470 480 490 500
THTYLDVTNI APAFTQRPVD TTVTDGMTAV LRCEVSGAPK PAITWKRGNH
510 520 530 540 550
ILASGSVRIP RFMLLESGGL RIAPVFIQDA GNYTCYAANT EASVNASAML
560 570 580 590 600
TVWNRTSIVH PPEDRVVIKG TTATLCCGAT HDPRTSLRYV WKKDNVVITA
610 620 630 640 650
SSSSRIVVEK DGSLVISQTW SGDIGDYTCE IISEGGSDSR TARLEVIELP
660 670 680 690 700
HPPQNLLASL SPARSHSVTL SWVRPFDGNS PVLYYIVQVS ENNSPWKVHL
710 720 730 740 750
SNVGPEMTGV TVSGLTPART YQFRVCAVNQ VGKGQYSTET SRLMLPEEPP
760 770 780 790 800
SAPPKNIVAS GRTNQSIMVQ WQPPPETEHN GVLRGYILRY RLAGLPGEHQ
810 820 830 840 850
QRNISSPEVN YCLVTDLIIW TQYEIQVAAY NGAGLGVFSR AVTEYTLQGV
860 870 880 890 900
PTAPPQNVQA EAVNSTTVHF LWNPPPQQFI NGINQGYKLL AWPADAPETV
910 920 930 940 950
TVVTIAPDFH GIHHGYITNL KKFTAYFTSV LCFTTPGDGP PSSPQLVWTH
960 970 980 990 1000
EDKPGAVGHL SFTEILDTSL KVSWQEPLER NGIIMGYQIS WEVYGRNDSR
1010 1020 1030 1040 1050
LTHTLNSTMH EYKIQGLSSL TTYTIDVAAL TAAGVGVTTS STISSGVPPD
1060 1070 1080 1090 1100
LPGAPSNLVI SNISPRSATL QFRPGYDGKT AICRWIVEGQ VGAIGDEEEW
1110 1120 1130 1140 1150
VTLYEEENEP DAQMLEIPNL TPYTHYRFRM RQVNIVGPSP FSQSSRVIQT
1160 1170 1180 1190 1200
LQAPPDVAPT SLTVRTASET SLRLRWVPLP DSQYNGNPES VGYRVKYWRS
1210 1220 1230 1240 1250
DQPSSALAQV VSDRLERELT IEELEEWTEY ELRMQAFNAI GAGPWSELVR
1260 1270 1280 1290 1300
GRTRESVPSA APENVSAEAV SSTQILLTWA SVPEQDQNGL ILGYKVLYCA
1310 1320 1330 1340 1350
KDLDPEPRSH VVRGNHTQSA LLAGLRKFVV YELQVLAFTR IGNGVPSSPL
1360 1370 1380 1390 1400
ILERTKDDTP GPPVRLVFPE VRLTAVRIVW QPPEEPNGVI LGYQIAYRLA
1410 1420 1430 1440 1450
SGSPHTFTTV EVGATVRQFT ATELAPESAY IFRLSAKTRQ GWGEPLEATV
1460 1470 1480 1490 1500
ITTEKRERPA PPRELLVPQA EVTARSLRLQ WVPGSDGASP IRYFTVQVRE
1510 1520 1530 1540 1550
LPGGEWQTYS SSISHEATAC AVERLRPFTS YKLRLKATND IGDSDFSAET
1560 1570 1580 1590 1600
EAVTTLQDVP GEPPGSVSAT PHTTSSVLIQ WQPPRDESLN GLLQGYRIYY
1610 1620 1630 1640 1650
RELESETGMS PEPKTLKSPS ALRAELTAQS SFKTVNSSSS LTTYELTHLK
1660 1670 1680 1690 1700
KYRRYEVIMT AYNIIGESPA SVPVEVFVGE AAPAMAPQNV QVTPLTASQL
1710 1720 1730 1740 1750
EVTWDPPPPE SQNGNIQGYK VYYWEADSRN ETEKMKVLFL PEPVVKIKDL
1760 1770 1780 1790 1800
TSHTKYLISI SAFNAAGDGP KSDPCQGRTH QAAPGPPSFL AFSEITSTTL
1810 1820 1830 1840 1850
NVSWGEPSAA NGILQGYRVV YEPLAPVQGV SKVVTVDVKG NWQRWLKVRD
1860 1870 1880 1890 1900
LTKGVTYFFR VQARTIAYGP ELQANVTAGP AEGSPGSPRN VLVTKSASEL
1910 1920 1930 1940 1950
TLQWTEGNAG TTPTTGYVIE ARPSDEGLWD MFAKDIPRSA TSYTVDLDKL
1960 1970 1980 1990 2000
RQGVTYEFRV VAVNKAGFGE PSRPSIAVSA QAEAPFYEEW WFLLVMALSS
2010 2020 2030 2040 2050
LLLILLVVFV LVLHGQSKKY KSCSTGKGIS NMEETVTLDN GGFAALELNS
2060 2070 2080 2090 2100
RHLNVKSTFS KKNGTRSPPR PSPGGLHYSD EDICNKYNGA VLTESVNLKE
2110 2120 2130 2140 2150
KSVDGSESEA SDSDYEEALP KHSFVNHYMS DPTYYNSWKR RPPAAAPHRY
2160 2170 2180 2190
EAVAGAEAGP HLHTVITTQS AGGVYTPAGP GARAPLTGFS SFV
Length:2,193
Mass (Da):240,306
Last modified:October 11, 2005 - v1
Checksum:iC22752D48DC4B137
GO
Isoform 2 (identifier: Q3UH53-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-260: Missing.
     261-265: LSVAR → MDRSG

Show »
Length:1,933
Mass (Da):211,606
Checksum:iB8AEF51A28AAC145
GO

Sequence cautioni

The sequence BAC28069 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC28986 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1009M → T in AAQ57662 (PubMed:15213259).Curated1
Sequence conflicti1888P → L in BAE28630 (PubMed:16141072).Curated1
Sequence conflicti1957E → G in BAC28986 (PubMed:16141072).Curated1
Sequence conflicti2038L → Q in BAE28630 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0175201 – 260Missing in isoform 2. 1 PublicationAdd BLAST260
Alternative sequenceiVSP_017521261 – 265LSVAR → MDRSG in isoform 2. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY353236 mRNA Translation: AAQ57662.1
AK032883 mRNA Translation: BAC28069.1 Different initiation.
AK035225 mRNA Translation: BAC28986.1 Different initiation.
AK147578 mRNA Translation: BAE28004.1
AK148648 mRNA Translation: BAE28630.1
BC060237 mRNA Translation: AAH60237.1
CCDSiCCDS39360.2 [Q3UH53-1]
RefSeqiNP_808547.3, NM_177879.5 [Q3UH53-1]
UniGeneiMm.151931
Mm.406697

Genome annotation databases

EnsembliENSMUST00000074546; ENSMUSP00000074133; ENSMUSG00000039683 [Q3UH53-2]
ENSMUST00000085774; ENSMUSP00000082928; ENSMUSG00000039683 [Q3UH53-1]
GeneIDi330222
KEGGimmu:330222
UCSCiuc009ail.2 mouse [Q3UH53-1]
uc009ain.2 mouse [Q3UH53-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY353236 mRNA Translation: AAQ57662.1
AK032883 mRNA Translation: BAC28069.1 Different initiation.
AK035225 mRNA Translation: BAC28986.1 Different initiation.
AK147578 mRNA Translation: BAE28004.1
AK148648 mRNA Translation: BAE28630.1
BC060237 mRNA Translation: AAH60237.1
CCDSiCCDS39360.2 [Q3UH53-1]
RefSeqiNP_808547.3, NM_177879.5 [Q3UH53-1]
UniGeneiMm.151931
Mm.406697

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5K6UX-ray2.20A78-459[»]
5K6VX-ray3.21A78-459[»]
5K6WX-ray3.50A/B78-556[»]
5K6ZX-ray2.70A/B268-459[»]
5XWXX-ray1.55A/B1-458[»]
ProteinModelPortaliQ3UH53
SMRiQ3UH53
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082928

PTM databases

iPTMnetiQ3UH53
PhosphoSitePlusiQ3UH53

Proteomic databases

PaxDbiQ3UH53
PeptideAtlasiQ3UH53
PRIDEiQ3UH53

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074546; ENSMUSP00000074133; ENSMUSG00000039683 [Q3UH53-2]
ENSMUST00000085774; ENSMUSP00000082928; ENSMUSG00000039683 [Q3UH53-1]
GeneIDi330222
KEGGimmu:330222
UCSCiuc009ail.2 mouse [Q3UH53-1]
uc009ain.2 mouse [Q3UH53-2]

Organism-specific databases

CTDi221935
MGIiMGI:2444413 Sdk1

Phylogenomic databases

eggNOGiKOG3510 Eukaryota
ENOG410ZHW1 LUCA
GeneTreeiENSGT00910000144009
HOGENOMiHOG000231052
HOVERGENiHBG080083
InParanoidiQ3UH53
KOiK16353
OMAiPETEHNG
OrthoDBiEOG091G0607
PhylomeDBiQ3UH53
TreeFamiTF316846

Enzyme and pathway databases

ReactomeiR-MMU-373756 SDK interactions

Miscellaneous databases

ChiTaRSiSdk1 mouse
PROiPR:Q3UH53
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039683 Expressed in 124 organ(s), highest expression level in embryo
CleanExiMM_SDK1
GenevisibleiQ3UH53 MM

Family and domain databases

CDDicd00063 FN3, 13 hits
Gene3Di2.60.40.10, 19 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
PfamiView protein in Pfam
PF00041 fn3, 13 hits
PF07679 I-set, 4 hits
SMARTiView protein in SMART
SM00060 FN3, 13 hits
SM00409 IG, 6 hits
SM00408 IGc2, 5 hits
SUPFAMiSSF48726 SSF48726, 6 hits
SSF49265 SSF49265, 7 hits
PROSITEiView protein in PROSITE
PS50853 FN3, 13 hits
PS50835 IG_LIKE, 5 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSDK1_MOUSE
AccessioniPrimary (citable) accession number: Q3UH53
Secondary accession number(s): Q3UFD1
, Q6PAL2, Q6V3A4, Q8BMC2, Q8BZI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: October 11, 2005
Last modified: October 10, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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