UniProtKB - Q3UGX3 (NAT8L_MOUSE)
Protein
N-acetylaspartate synthetase
Gene
Nat8l
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake.4 Publications
Miscellaneous
Seems to modulate behavioral effects induced by methamphetamine in vivo.
'Shati' is the name of the symbol at Nagoya castle in Japan.
Catalytic activityi
- EC:2.3.1.171 Publication
Activity regulationi
Aminooxyacetic acid (AOAA) blocks its activity in both cytoplasm and mitochondria.By similarity
GO - Molecular functioni
- aspartate N-acetyltransferase activity Source: UniProtKB
- N-acetyltransferase activity Source: GO_Central
GO - Biological processi
- positive regulation of dopamine uptake involved in synaptic transmission Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.3.1.17, 3474 |
Reactomei | R-MMU-8963693, Aspartate and asparagine metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: N-acetylaspartate synthetase (EC:2.3.1.17)Short name: NAA synthetase Alternative name(s): N-acetyltransferase 8-like protein Protein Shati |
Gene namesi | Name:Nat8l |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2447776, Nat8l |
Subcellular locationi
Endoplasmic reticulum
- Microsome membrane By similarity; Single-pass membrane protein By similarity
- Rough endoplasmic reticulum membrane ; Single-pass membrane protein
Mitochondrion
- Mitochondrion membrane By similarity; Single-pass membrane protein By similarity
Other locations
- Cytoplasm By similarity
- Membrane Curated; Single-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
- rough endoplasmic reticulum membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial membrane Source: MGI
- mitochondrion Source: MGI
Other locations
- cytoplasm Source: MGI
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 118 – 138 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000305230 | 1 – 299 | N-acetylaspartate synthetaseAdd BLAST | 299 |
Proteomic databases
MaxQBi | Q3UGX3 |
PaxDbi | Q3UGX3 |
PeptideAtlasi | Q3UGX3 |
PRIDEi | Q3UGX3 |
PTM databases
SwissPalmi | Q3UGX3 |
Expressioni
Tissue specificityi
Expressed in brain, kidney, liver and spleen. In brain, present in neurons but not in astrocytes (at protein level). Expressed in brain, thymus and spleen.3 Publications
Inductioni
By methamphetamine in brain, via dopamine receptor activation (at protein level).1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 234689, 1 interactor |
STRINGi | 10090.ENSMUSP00000059313 |
Miscellaneous databases
RNActi | Q3UGX3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 143 – 280 | N-acetyltransferasePROSITE-ProRule annotationAdd BLAST | 138 |
Sequence similaritiesi
Belongs to the camello family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3139, Eukaryota |
InParanoidi | Q3UGX3 |
OrthoDBi | 1341682at2759 |
PhylomeDBi | Q3UGX3 |
TreeFami | TF324687 |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q3UGX3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHCGPPDMVC ETKIVATEDH EALPGAKKDA LLVAAGAMWP PLPAAPGPAA
60 70 80 90 100
APPPAAGPQP HGGTGGAGPP EGRGVCIREF RAAEQEAARR IFYDGILERI
110 120 130 140 150
PNTAFRGLRQ HPRTQLLYAL LAALCFAVTR SLLLTCLVPA GLLALRYYYS
160 170 180 190 200
RKVILAYLEC ALHTDMADIE QYYMKPPGSC FWVAVLDGNV VGIVAARAHE
210 220 230 240 250
EDNTVELLRM SVDSRFRGKS IAKALGRRVL EFAMLHNYSA VVLGTTAVKV
260 270 280 290
AAHKLYESLG FRHMGASDHY VLPGMTLSLA ERLFFQVRYH RYRLQLREE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J0R4 | A0A0R4J0R4_MOUSE | N-acetylaspartate synthetase | Nat8l | 299 | Annotation score: |
Sequence cautioni
The sequence AAH75616 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence ABA54615 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 220 | S → G in BAE28014 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 220 | S → G in ABA54615 (PubMed:17626222).Curated | 1 | |
Sequence conflicti | 220 | S → G in AAH75616 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK147307 mRNA Translation: BAE27836.1 AK147594 mRNA Translation: BAE28014.1 AK147703 mRNA Translation: BAE28084.1 DQ174094 mRNA Translation: ABA54615.1 Different initiation. BC034068 mRNA Translation: AAH34068.1 BC075616 mRNA Translation: AAH75616.1 Different initiation. |
CCDSi | CCDS19210.1 |
RefSeqi | NP_001001985.3, NM_001001985.3 |
Genome annotation databases
GeneIDi | 269642 |
KEGGi | mmu:269642 |
UCSCi | uc008xbs.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK147307 mRNA Translation: BAE27836.1 AK147594 mRNA Translation: BAE28014.1 AK147703 mRNA Translation: BAE28084.1 DQ174094 mRNA Translation: ABA54615.1 Different initiation. BC034068 mRNA Translation: AAH34068.1 BC075616 mRNA Translation: AAH75616.1 Different initiation. |
CCDSi | CCDS19210.1 |
RefSeqi | NP_001001985.3, NM_001001985.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 234689, 1 interactor |
STRINGi | 10090.ENSMUSP00000059313 |
PTM databases
SwissPalmi | Q3UGX3 |
Proteomic databases
MaxQBi | Q3UGX3 |
PaxDbi | Q3UGX3 |
PeptideAtlasi | Q3UGX3 |
PRIDEi | Q3UGX3 |
Genome annotation databases
GeneIDi | 269642 |
KEGGi | mmu:269642 |
UCSCi | uc008xbs.1, mouse |
Organism-specific databases
CTDi | 339983 |
MGIi | MGI:2447776, Nat8l |
Phylogenomic databases
eggNOGi | KOG3139, Eukaryota |
InParanoidi | Q3UGX3 |
OrthoDBi | 1341682at2759 |
PhylomeDBi | Q3UGX3 |
TreeFami | TF324687 |
Enzyme and pathway databases
BRENDAi | 2.3.1.17, 3474 |
Reactomei | R-MMU-8963693, Aspartate and asparagine metabolism |
Miscellaneous databases
BioGRID-ORCSi | 269642, 1 hit in 19 CRISPR screens |
ChiTaRSi | Nat8l, mouse |
PROi | PR:Q3UGX3 |
RNActi | Q3UGX3, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NAT8L_MOUSE | |
Accessioni | Q3UGX3Primary (citable) accession number: Q3UGX3 Secondary accession number(s): Q3UH43, Q6DID6, Q8K065 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | October 11, 2005 | |
Last modified: | December 2, 2020 | |
This is version 102 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families