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Protein

Glutathione synthetase

Gene

Gss

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase regulatory subunit (Gclm), Glutamate--cysteine ligase catalytic subunit (Gclc)
  2. Glutathione synthetase (Gss), Glutathione synthetase (Gss), Glutathione synthetase (Gss)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56SubstrateUniRule annotation1
Metal bindingi75MagnesiumUniRule annotation1
Binding sitei75ATPUniRule annotation1
Metal bindingi77MagnesiumUniRule annotation1
Binding sitei151SubstrateUniRule annotation1
Binding sitei236ATPUniRule annotation1
Metal bindingi299MagnesiumUniRule annotation1
Binding sitei306ATPUniRule annotation1
Binding sitei356ATPUniRule annotation1
Binding sitei381SubstrateUniRule annotation1
Binding sitei383ATPUniRule annotation1
Binding sitei389ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi295 – 304ATPUniRule annotation10
Nucleotide bindingi329 – 332ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotation
Biological processGlutathione biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00142;UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Short name:
GSH-SUniRule annotation
Gene namesi
Name:GssImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95852 Gss

Expressioni

Gene expression databases

BgeeiENSMUSG00000027610 Expressed in 241 organ(s), highest expression level in lens of camera-type eye

Interactioni

GO - Molecular functioni

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini136 – 233GSH_synthaseInterPro annotationAdd BLAST98

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili303 – 323Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiKOG0021 Eukaryota
ENOG410XPHH LUCA
GeneTreeiENSGT00390000013764
HOVERGENiHBG002458

Family and domain databases

Gene3Di1.10.1080.10, 2 hits
3.30.1490.50, 1 hit
3.30.1490.80, 2 hits
3.40.50.1760, 1 hit
InterProiView protein in InterPro
IPR005615 Glutathione_synthase
IPR014042 Glutathione_synthase_a-hlx
IPR014709 Glutathione_synthase_C_euk
IPR014049 Glutathione_synthase_N_euk
IPR037013 GSH-S_sub-bd_sf
IPR004887 GSH_synth_subst-bd
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR11130 PTHR11130, 1 hit
PfamiView protein in Pfam
PF03917 GSH_synth_ATP, 1 hit
PF03199 GSH_synthase, 1 hit
PIRSFiPIRSF001558 GSHase, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01986 glut_syn_euk, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q3UEE2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDFNILVDAV SQNPAFLEQT LSSTIKKDDY TARLFDIYKQ VLKEGIAQTV
60 70 80 90 100
FLGLNRSDYM FQCGADGSKA LKQIEINTIS ASFGGLASRT PAVHRHVLNV
110 120 130 140 150
LNKTKEASKI LSNNPSKGLA LGIAKAWELY GSANAVVLLI AQEKERNIFD
160 170 180 190 200
QRAVENELLD RKIHVIRGRF EDVSERGSLD QNRRLFMDDQ EVAVVYFRDG
210 220 230 240 250
YMPSQYNSQN WEARLMLERS RAAKCPDIAI QLAGTKKVQQ ELSRVGLLEA
260 270 280 290 300
LLPGQPEAVA RLRATFAGLY SLDMGEEGDQ AIAEALAAPS HFVLKPQREG
310 320 330 340 350
GGNNLYGEEM VQALEQLKDS EERASYILME KIEPEPFRNC LLRPGSPAQV
360 370 380 390 400
VQCISELGIF GVYVRQGTTL VMNKHVGHLL RTKAVEHADG GVAAGVAVLD

NPYPV
Length:405
Mass (Da):44,792
Last modified:October 11, 2005 - v1
Checksum:i844C34DE89A8A683
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
P51855GSHB_MOUSE
Glutathione synthetase
Gss
474Annotation score:
A2AQN9A2AQN9_MOUSE
Glutathione synthetase
Gss
179Annotation score:
H3BKH4H3BKH4_MOUSE
Glutathione synthetase
Gss
216Annotation score:
A2AQN7A2AQN7_MOUSE
Glutathione synthetase
Gss
200Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844852 Genomic DNA No translation available.
AK149577 mRNA Translation: BAE28969.1
RefSeqiNP_001278040.1, NM_001291111.1
UniGeneiMm.252316

Genome annotation databases

EnsembliENSMUST00000130881; ENSMUSP00000135319; ENSMUSG00000027610
GeneIDi14854

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844852 Genomic DNA No translation available.
AK149577 mRNA Translation: BAE28969.1
RefSeqiNP_001278040.1, NM_001291111.1
UniGeneiMm.252316

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000130881; ENSMUSP00000135319; ENSMUSG00000027610
GeneIDi14854

Organism-specific databases

CTDi2937
MGIiMGI:95852 Gss

Phylogenomic databases

eggNOGiKOG0021 Eukaryota
ENOG410XPHH LUCA
GeneTreeiENSGT00390000013764
HOVERGENiHBG002458

Enzyme and pathway databases

UniPathwayi
UPA00142;UER00210

Miscellaneous databases

SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027610 Expressed in 241 organ(s), highest expression level in lens of camera-type eye

Family and domain databases

Gene3Di1.10.1080.10, 2 hits
3.30.1490.50, 1 hit
3.30.1490.80, 2 hits
3.40.50.1760, 1 hit
InterProiView protein in InterPro
IPR005615 Glutathione_synthase
IPR014042 Glutathione_synthase_a-hlx
IPR014709 Glutathione_synthase_C_euk
IPR014049 Glutathione_synthase_N_euk
IPR037013 GSH-S_sub-bd_sf
IPR004887 GSH_synth_subst-bd
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR11130 PTHR11130, 1 hit
PfamiView protein in Pfam
PF03917 GSH_synth_ATP, 1 hit
PF03199 GSH_synthase, 1 hit
PIRSFiPIRSF001558 GSHase, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01986 glut_syn_euk, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiQ3UEE2_MOUSE
AccessioniPrimary (citable) accession number: Q3UEE2
Entry historyiIntegrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: October 10, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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