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UniProtKB - Q3U9F6 (RN166_MOUSE)
Protein
E3 ubiquitin-protein ligase RNF166
Gene
Rnf166
Organism
Mus musculus (Mouse)
Status
Functioni
E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates. In turn, participates in different biological processes including interferon production or autophagy. Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6. Plays also a role in the early recruitment of autophagy adapters to bacteria. Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication.
By similarityCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 98 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 101 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 113 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 117 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 33 – 73 | RING-typePROSITE-ProRule annotationAdd BLAST | 41 | |
Zinc fingeri | 98 – 117 | C2HC RNF-typePROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: GO_Central
GO - Biological processi
- autophagy Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- protein polyubiquitination Source: GO_Central
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Autophagy, Immunity, Innate immunity, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RNF166 (EC:2.3.2.27)Alternative name(s): RING finger protein 166 RING-type E3 ubiquitin transferase RNF166 |
Gene namesi | Name:Rnf166 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915968, Rnf166 |
VEuPathDBi | HostDB:ENSMUSG00000014470 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245589 | 1 – 237 | E3 ubiquitin-protein ligase RNF166Add BLAST | 237 |
Proteomic databases
MaxQBi | Q3U9F6 |
PaxDbi | Q3U9F6 |
PRIDEi | Q3U9F6 |
ProteomicsDBi | 300416 |
PTM databases
iPTMneti | Q3U9F6 |
PhosphoSitePlusi | Q3U9F6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000014470, Expressed in thymus and 311 other tissues |
ExpressionAtlasi | Q3U9F6, baseline and differential |
Genevisiblei | Q3U9F6, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 213009, 2 interactors |
STRINGi | 10090.ENSMUSP00000014614 |
Miscellaneous databases
RNActi | Q3U9F6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 221 – 237 | UIMCuratedAdd BLAST | 17 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 33 – 73 | RING-typePROSITE-ProRule annotationAdd BLAST | 41 | |
Zinc fingeri | 98 – 117 | C2HC RNF-typePROSITE-ProRule annotationAdd BLAST | 20 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502RB47, Eukaryota |
GeneTreei | ENSGT00950000182909 |
HOGENOMi | CLU_092448_1_0_1 |
InParanoidi | Q3U9F6 |
OMAi | FCGDCLQ |
OrthoDBi | 1097558at2759 |
PhylomeDBi | Q3U9F6 |
TreeFami | TF331012 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR008598, Di19_Zn-bd IPR034734, ZF_C2HC_RNF IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF05605, zf-Di19, 1 hit PF18574, zf_C2HC_14, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS51803, ZF_C2HC_RNF, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q3U9F6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAMFRSLVAS AQQRQPPAGP AGGDSGLEAQ FSCPICLEVY HRPVAIGSCG
60 70 80 90 100
HTFCGECLQP CLQVPSPLCP LCRLPFDPKK VDKATHVEKQ LSSYKAPCRG
110 120 130 140 150
CNKKVTLAKM RAHISSCLKV QEQMANCPKF VPVVPTSQPI PSNIPNRSTF
160 170 180 190 200
ACPYCGARNL DQQELVKHCV ESHRSDPNRV VCPICSAMPW GDPSYKSANF
210 220 230
LQHLLHRHKF SYDTFVDYSI DEEAAFQAAL ALSLSEN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK151813 mRNA Translation: BAE30711.1 |
CCDSi | CCDS22740.1 |
RefSeqi | NP_001028314.1, NM_001033142.2 |
Genome annotation databases
Ensembli | ENSMUST00000014614; ENSMUSP00000014614; ENSMUSG00000014470 |
GeneIDi | 68718 |
KEGGi | mmu:68718 |
UCSCi | uc009nsx.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK151813 mRNA Translation: BAE30711.1 |
CCDSi | CCDS22740.1 |
RefSeqi | NP_001028314.1, NM_001033142.2 |
3D structure databases
AlphaFoldDBi | Q3U9F6 |
SMRi | Q3U9F6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 213009, 2 interactors |
STRINGi | 10090.ENSMUSP00000014614 |
PTM databases
iPTMneti | Q3U9F6 |
PhosphoSitePlusi | Q3U9F6 |
Proteomic databases
MaxQBi | Q3U9F6 |
PaxDbi | Q3U9F6 |
PRIDEi | Q3U9F6 |
ProteomicsDBi | 300416 |
Protocols and materials databases
Antibodypediai | 30751, 125 antibodies from 20 providers |
Genome annotation databases
Ensembli | ENSMUST00000014614; ENSMUSP00000014614; ENSMUSG00000014470 |
GeneIDi | 68718 |
KEGGi | mmu:68718 |
UCSCi | uc009nsx.1, mouse |
Organism-specific databases
CTDi | 115992 |
MGIi | MGI:1915968, Rnf166 |
VEuPathDBi | HostDB:ENSMUSG00000014470 |
Phylogenomic databases
eggNOGi | ENOG502RB47, Eukaryota |
GeneTreei | ENSGT00950000182909 |
HOGENOMi | CLU_092448_1_0_1 |
InParanoidi | Q3U9F6 |
OMAi | FCGDCLQ |
OrthoDBi | 1097558at2759 |
PhylomeDBi | Q3U9F6 |
TreeFami | TF331012 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
BioGRID-ORCSi | 68718, 1 hit in 74 CRISPR screens |
ChiTaRSi | Rnf166, mouse |
PROi | PR:Q3U9F6 |
RNActi | Q3U9F6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000014470, Expressed in thymus and 311 other tissues |
ExpressionAtlasi | Q3U9F6, baseline and differential |
Genevisiblei | Q3U9F6, MM |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR008598, Di19_Zn-bd IPR034734, ZF_C2HC_RNF IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF05605, zf-Di19, 1 hit PF18574, zf_C2HC_14, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS51803, ZF_C2HC_RNF, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RN166_MOUSE | |
Accessioni | Q3U9F6Primary (citable) accession number: Q3U9F6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | October 11, 2005 | |
Last modified: | May 25, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways