UniProtKB - Q3U7R1 (ESYT1_MOUSE)
Extended synaptotagmin-1
Esyt1
Functioni
Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity).
Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 334 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 335 | Calcium 1By similarity | 1 | |
Metal bindingi | 335 | Calcium 2By similarity | 1 | |
Metal bindingi | 347 | Calcium 2By similarity | 1 | |
Metal bindingi | 394 | Calcium 1By similarity | 1 | |
Metal bindingi | 394 | Calcium 2By similarity | 1 | |
Metal bindingi | 396 | Calcium 1By similarity | 1 | |
Metal bindingi | 396 | Calcium 2By similarity | 1 | |
Metal bindingi | 396 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 398 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 400 | Calcium 3By similarity | 1 | |
Metal bindingi | 401 | Calcium 1By similarity | 1 |
GO - Molecular functioni
- calcium-dependent phospholipid binding Source: GO_Central
- calcium ion binding Source: GO_Central
- identical protein binding Source: MGI
- phosphatidylcholine binding Source: GO_Central
- phosphatidylethanolamine binding Source: GO_Central
- phosphatidylinositol binding Source: GO_Central
GO - Biological processi
- endoplasmic reticulum-plasma membrane tethering Source: InterPro
- lipid transport Source: UniProtKB-KW
Keywordsi
Biological process | Lipid transport, Transport |
Ligand | Calcium, Lipid-binding, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1660662, Glycosphingolipid metabolism R-MMU-9013149, RAC1 GTPase cycle R-MMU-9013404, RAC2 GTPase cycle R-MMU-9013405, RHOD GTPase cycle R-MMU-9013408, RHOG GTPase cycle R-MMU-9013423, RAC3 GTPase cycle R-MMU-9035034, RHOF GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Extended synaptotagmin-1Short name: E-Syt1 Alternative name(s): Membrane-bound C2 domain-containing protein |
Gene namesi | Name:Esyt1 Synonyms:Fam62a, Mbc2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1344426, Esyt1 |
VEuPathDBi | HostDB:ENSMUSG00000025366 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Note: Localizes primarily to the endoplasmic reticulum. Recruited to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- integral component of endoplasmic reticulum membrane Source: UniProtKB
- intrinsic component of endoplasmic reticulum membrane Source: GO_Central
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 28 | CytoplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 29 – 49 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 50 – 52 | LumenalSequence analysis | 3 | |
Transmembranei | 53 – 73 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 74 – 1092 | CytoplasmicSequence analysisAdd BLAST | 1019 |
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 314 | S → A: Abolished phosphorylation by CDK5. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3621032 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000234345 | 1 – 1092 | Extended synaptotagmin-1Add BLAST | 1092 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 314 | Phosphoserine; by CDK51 Publication | 1 | |
Modified residuei | 804 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 807 | PhosphoserineBy similarity | 1 | |
Modified residuei | 937 | PhosphoserineBy similarity | 1 | |
Modified residuei | 951 | PhosphoserineCombined sources | 1 | |
Modified residuei | 997 | PhosphotyrosineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q3U7R1 |
jPOSTi | Q3U7R1 |
MaxQBi | Q3U7R1 |
PaxDbi | Q3U7R1 |
PeptideAtlasi | Q3U7R1 |
PRIDEi | Q3U7R1 |
ProteomicsDBi | 275692 [Q3U7R1-1] 275693 [Q3U7R1-2] |
PTM databases
iPTMneti | Q3U7R1 |
PhosphoSitePlusi | Q3U7R1 |
SwissPalmi | Q3U7R1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000025366, Expressed in white adipose tissue and 118 other tissues |
Genevisiblei | Q3U7R1, MM |
Interactioni
Subunit structurei
Interacts with ESYT2 and ESYT3 (By similarity).
Interacts (phosphorylated form) with SLC2A4.
By similarity1 PublicationBinary interactionsi
Q3U7R1
With | #Exp. | IntAct |
---|---|---|
Slc2a4 [P14142] | 2 | EBI-8398881,EBI-7540210 |
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204808, 10 interactors |
DIPi | DIP-48746N |
IntActi | Q3U7R1, 5 interactors |
MINTi | Q3U7R1 |
STRINGi | 10090.ENSMUSP00000026427 |
Miscellaneous databases
RNActi | Q3U7R1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 125 – 303 | SMP-LTDPROSITE-ProRule annotationAdd BLAST | 179 | |
Domaini | 302 – 423 | C2 1PROSITE-ProRule annotationAdd BLAST | 122 | |
Domaini | 444 – 570 | C2 2PROSITE-ProRule annotationAdd BLAST | 127 | |
Domaini | 616 – 738 | C2 3PROSITE-ProRule annotationAdd BLAST | 123 | |
Domaini | 769 – 886 | C2 4PROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 959 – 1081 | C2 5PROSITE-ProRule annotationAdd BLAST | 123 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 36 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 604 – 628 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 909 – 937 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 1006 – 1013 | Required for phosphatidylinositol 4,5-bisphosphate-dependent location at the cell membraneBy similarity | 8 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 909 – 926 | Polar residuesSequence analysisAdd BLAST | 18 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1012, Eukaryota |
GeneTreei | ENSGT00940000156561 |
HOGENOMi | CLU_012047_0_0_1 |
InParanoidi | Q3U7R1 |
OMAi | HPGQSKE |
OrthoDBi | 52746at2759 |
PhylomeDBi | Q3U7R1 |
TreeFami | TF324255 |
Family and domain databases
CDDi | cd08391, C2A_C2C_Synaptotagmin_like, 2 hits cd04050, C2B_Synaptotagmin-like, 2 hits cd04030, C2C_KIAA1228, 1 hit |
Gene3Di | 2.60.40.150, 5 hits |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR037752, C2C_KIAA1228 IPR037733, Ext_Synaptotagmin_C2A IPR037749, Ext_Synaptotagmin_C2B IPR031468, SMP_LBD IPR039010, Synaptotagmin_SMP |
Pfami | View protein in Pfam PF00168, C2, 5 hits PF17047, SMP_LBD, 1 hit |
PRINTSi | PR00360, C2DOMAIN |
SMARTi | View protein in SMART SM00239, C2, 5 hits |
SUPFAMi | SSF49562, SSF49562, 5 hits |
PROSITEi | View protein in PROSITE PS50004, C2, 5 hits PS51847, SMP, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEHSPEEGAS PEPSGQPPAT DSTRDGGSGV PPAGPGAASE ALAVLTSFGR
60 70 80 90 100
RLLVLVPVYL AGAAGLSVGF VLFGLALYLG WRRVRDGKER SLRAARQLLD
110 120 130 140 150
DEERITAETL YMSHRELPAW VSFPDVEKAE WLNKIVAQVW PFLGQYMEKL
160 170 180 190 200
LAETVAPAVR GANPHLQTFT FTRVELGEKP LRIIGVKVHP SQRKDQILLD
210 220 230 240 250
LNVSYVGDVQ IDVEVKKYFC KAGVKGMQLH GVLRVILEPL TGDLPIVGAV
260 270 280 290 300
SMFFIKRPTL DINWTGMTNL LDIPGLSSLS DTMIMDSIAA FLVLPNRLLV
310 320 330 340 350
PLVPDLQDVA QLRSPLPRGI IRIHLLAARG LSSKDKYVKG LIEGKSDPYA
360 370 380 390 400
LVRVGTQTFC SRVIDEELNP HWGETYEVIV HEVPGQEIEV EVFDKDPDKD
410 420 430 440 450
DFLGRMKLDV GKVLQAGVLD NWYPLQGGQG QVHLRLEWLS LLPDAEKLDQ
460 470 480 490 500
VLQWNRGITS RPEPPSAAIL VVYLDRAQDL PLKKGNKEPN PMVQLSVQDV
510 520 530 540 550
TRESKATYST NSPVWEEAFR FFLQDPRSQE LDVQVKDDSR ALTLGALTLP
560 570 580 590 600
LARLLTASEL TLDQWFQLSS SGPNSRLYMK LVMRILYLDY SEIRFPTVPG
610 620 630 640 650
AQDWDRESLE TGSSVDAPPR PYHTTPNSHF GTENVLRIHV LEAQDLIAKD
660 670 680 690 700
RFLGGLVKGK SDPYVKLKVA GKSFRTHVVR EDLNPRWNEV FEVIVTSIPG
710 720 730 740 750
QELEIEVFDK DLDKDDFLGR YKVSLTTVLN SGFLDEWLTL EDVPSGRLHL
760 770 780 790 800
RLERLTPRPT AAELEEVLQV NSLIQTQKSS ELAAALLSVF LERAEDLPLR
810 820 830 840 850
KGTKPPSPYA TITVGETSHK TKTVSQSSAP VWEESASFLI RKPHAESLEL
860 870 880 890 900
QVRGEGTGTL GSVSLPLSEL LQEDQLCLDH WFALSGQGQV LMRAQLGILV
910 920 930 940 950
SQHSGVEAHS HSYSHSHSSS SLNDEPEALG GPTHPASPVL EVRHRLTHGD
960 970 980 990 1000
SPSEAPVGPL GQVKLTVWYH SDEQKLISII HSCRALRQNG RDLPDPYVSV
1010 1020 1030 1040 1050
LLLPDKNRST KRKTPQKKRT LNPEFNERFE WDLPLDGTLR RKLDVSVKSN
1060 1070 1080 1090
SSFMSREREL LGKVQLDLAE IDLSQGAAQW YDLMDDRDKG GS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P784 | A0A1W2P784_MOUSE | Extended synaptotagmin-1 | Esyt1 | 528 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 385 | G → R in BAC32020 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 392 – 394 | VFD → IQH in AAD10190 (PubMed:10350628).Curated | 3 | |
Sequence conflicti | 829 – 841 | APVWE…SFLIR → GFGLEAKIRHEGG in AAD10189 (PubMed:10350628).CuratedAdd BLAST | 13 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018278 | 852 – 893 | VRGEG…QVLMR → ACFIHLLFLFERGREQRTVF EFEVFGVNSFPPCGNLWATA HS in isoform 2. 1 PublicationAdd BLAST | 42 | |
Alternative sequenceiVSP_018279 | 894 – 1092 | Missing in isoform 2. 1 PublicationAdd BLAST | 199 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK008224 mRNA Translation: BAB25542.1 AK044656 mRNA Translation: BAC32020.1 AK152482 mRNA Translation: BAE31254.1 AK152554 mRNA Translation: BAE31308.1 BC011482 mRNA Translation: AAH11482.1 AF098633 mRNA Translation: AAD10189.1 AF098634 mRNA Translation: AAD10190.1 |
CCDSi | CCDS24280.1 [Q3U7R1-1] |
RefSeqi | NP_035973.1, NM_011843.2 [Q3U7R1-1] |
Genome annotation databases
Ensembli | ENSMUST00000026427; ENSMUSP00000026427; ENSMUSG00000025366 [Q3U7R1-1] |
GeneIDi | 23943 |
KEGGi | mmu:23943 |
UCSCi | uc007hng.1, mouse [Q3U7R1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK008224 mRNA Translation: BAB25542.1 AK044656 mRNA Translation: BAC32020.1 AK152482 mRNA Translation: BAE31254.1 AK152554 mRNA Translation: BAE31308.1 BC011482 mRNA Translation: AAH11482.1 AF098633 mRNA Translation: AAD10189.1 AF098634 mRNA Translation: AAD10190.1 |
CCDSi | CCDS24280.1 [Q3U7R1-1] |
RefSeqi | NP_035973.1, NM_011843.2 [Q3U7R1-1] |
3D structure databases
AlphaFoldDBi | Q3U7R1 |
SMRi | Q3U7R1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204808, 10 interactors |
DIPi | DIP-48746N |
IntActi | Q3U7R1, 5 interactors |
MINTi | Q3U7R1 |
STRINGi | 10090.ENSMUSP00000026427 |
Chemistry databases
ChEMBLi | CHEMBL3621032 |
PTM databases
iPTMneti | Q3U7R1 |
PhosphoSitePlusi | Q3U7R1 |
SwissPalmi | Q3U7R1 |
Proteomic databases
EPDi | Q3U7R1 |
jPOSTi | Q3U7R1 |
MaxQBi | Q3U7R1 |
PaxDbi | Q3U7R1 |
PeptideAtlasi | Q3U7R1 |
PRIDEi | Q3U7R1 |
ProteomicsDBi | 275692 [Q3U7R1-1] 275693 [Q3U7R1-2] |
Protocols and materials databases
Antibodypediai | 3015, 241 antibodies from 33 providers |
Genome annotation databases
Ensembli | ENSMUST00000026427; ENSMUSP00000026427; ENSMUSG00000025366 [Q3U7R1-1] |
GeneIDi | 23943 |
KEGGi | mmu:23943 |
UCSCi | uc007hng.1, mouse [Q3U7R1-1] |
Organism-specific databases
CTDi | 23344 |
MGIi | MGI:1344426, Esyt1 |
VEuPathDBi | HostDB:ENSMUSG00000025366 |
Phylogenomic databases
eggNOGi | KOG1012, Eukaryota |
GeneTreei | ENSGT00940000156561 |
HOGENOMi | CLU_012047_0_0_1 |
InParanoidi | Q3U7R1 |
OMAi | HPGQSKE |
OrthoDBi | 52746at2759 |
PhylomeDBi | Q3U7R1 |
TreeFami | TF324255 |
Enzyme and pathway databases
Reactomei | R-MMU-1660662, Glycosphingolipid metabolism R-MMU-9013149, RAC1 GTPase cycle R-MMU-9013404, RAC2 GTPase cycle R-MMU-9013405, RHOD GTPase cycle R-MMU-9013408, RHOG GTPase cycle R-MMU-9013423, RAC3 GTPase cycle R-MMU-9035034, RHOF GTPase cycle |
Miscellaneous databases
BioGRID-ORCSi | 23943, 5 hits in 75 CRISPR screens |
ChiTaRSi | Esyt1, mouse |
PROi | PR:Q3U7R1 |
RNActi | Q3U7R1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025366, Expressed in white adipose tissue and 118 other tissues |
Genevisiblei | Q3U7R1, MM |
Family and domain databases
CDDi | cd08391, C2A_C2C_Synaptotagmin_like, 2 hits cd04050, C2B_Synaptotagmin-like, 2 hits cd04030, C2C_KIAA1228, 1 hit |
Gene3Di | 2.60.40.150, 5 hits |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR037752, C2C_KIAA1228 IPR037733, Ext_Synaptotagmin_C2A IPR037749, Ext_Synaptotagmin_C2B IPR031468, SMP_LBD IPR039010, Synaptotagmin_SMP |
Pfami | View protein in Pfam PF00168, C2, 5 hits PF17047, SMP_LBD, 1 hit |
PRINTSi | PR00360, C2DOMAIN |
SMARTi | View protein in SMART SM00239, C2, 5 hits |
SUPFAMi | SSF49562, SSF49562, 5 hits |
PROSITEi | View protein in PROSITE PS50004, C2, 5 hits PS51847, SMP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ESYT1_MOUSE | |
Accessioni | Q3U7R1Primary (citable) accession number: Q3U7R1 Secondary accession number(s): Q8C8R1 Q9Z1X6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | May 16, 2006 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families