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Protein

E3 ubiquitin-protein ligase HECTD3

Gene

Hectd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP (By imilarity). Mediates also ubiquitination of STX8.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei823Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • syntaxin binding Source: UniProtKB
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HECTD3 (EC:2.3.2.26)
Alternative name(s):
HECT domain-containing protein 3
HECT-type E3 ubiquitin transferase HECTD3
Gene namesi
Name:Hectd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1923858 Hectd3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002414462 – 861E3 ubiquitin-protein ligase HECTD3Add BLAST860

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei12PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ3U487
MaxQBiQ3U487
PaxDbiQ3U487
PeptideAtlasiQ3U487
PRIDEiQ3U487

PTM databases

iPTMnetiQ3U487
PhosphoSitePlusiQ3U487

Expressioni

Gene expression databases

BgeeiENSMUSG00000046861
CleanExiMM_HECTD3
GenevisibleiQ3U487 MM

Interactioni

Subunit structurei

Interacts with TRIOBP (By similarity). Interacts with STX8.By similarity1 Publication

GO - Molecular functioni

  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051922

Structurei

3D structure databases

ProteinModelPortaliQ3U487
SMRiQ3U487
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini219 – 397DOCPROSITE-ProRule annotationAdd BLAST179
Domaini512 – 857HECTPROSITE-ProRule annotationAdd BLAST346

Phylogenomic databases

eggNOGiKOG0939 Eukaryota
COG5021 LUCA
GeneTreeiENSGT00910000144004
HOGENOMiHOG000007274
HOVERGENiHBG058297
InParanoidiQ3U487
KOiK12233
OMAiQPAEAYT
OrthoDBiEOG091G01ET
PhylomeDBiQ3U487

Family and domain databases

Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR004939 APC_su10/DOC_dom
IPR008979 Galactose-bd-like_sf
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
PfamiView protein in Pfam
PF03256 ANAPC10, 1 hit
PF00632 HECT, 1 hit
SMARTiView protein in SMART
SM01337 APC10, 1 hit
SM00119 HECTc, 1 hit
SUPFAMiSSF49785 SSF49785, 1 hit
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS51284 DOC, 1 hit
PS50237 HECT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3U487-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGPGPGAAL ESPRQLLGRV RFLAEAARSL RAGLPLPAAL AFVPREVLYK
60 70 80 90 100
LYKDPAGPSR VLLPVWEAEG LGLRVGAVGA APGTGSGPLR AARDSIELRR
110 120 130 140 150
GACVRTTGEE LCNGHGLWVK LTKEQLAEHL SDCSLDEGWL LVCRPAEGGA
160 170 180 190 200
RLVPIDTPDH LQRQQQLFGV DYRPVLRWEQ VVDLTYSHRL GSRPQPAEAY
210 220 230 240 250
TEAIQRLLYV PPTWTYECDE DLIHFLYDHL GKEDENLGSV KQYVESIDVS
260 270 280 290 300
SYTEEFNVSC LTDSNADTYW ESDGSQCQHW VRLTMKKGTI VKKLLLTVDT
310 320 330 340 350
TDDNFMPKRV VVYGGEGDNL KKLSDVNIDE TLIGDVCVLE DMTVHLPIIE
360 370 380 390 400
IRIVECRDDG IDVRLRGVKI KSSRQRELGL NADLFQPASL VRYPRLEGTD
410 420 430 440 450
PEVLYRRAVL LQRFIKILDS VLHHLVPAWD HTLGTFSEIK QVKQFLLLSR
460 470 480 490 500
QRPSLVAQCL RDSESSKPSF MPRLYINRRL AMEHRACPSR DPACKNAVFT
510 520 530 540 550
QVYEGLKPSD KYEKPLDYRW PMRYDQWWEC KFIAEGIIDQ GGGFRDSLAD
560 570 580 590 600
MSEELCPSSA DTPVPLPFFV RTANQGNGTG EARDMYVPNP SCRDFAKYEW
610 620 630 640 650
IGQLMGAALR GKEFLVLALP GFVWKQLSGE EVSWSKDFPA VDSVLVKLLE
660 670 680 690 700
VMEGVDKETF EFKFGKELTF TTVLSDQQVV ELIPGGTGIV VEYEDRSRFI
710 720 730 740 750
QLVRKARLEE SKEQVAAMQA GLLKVVPQAV LDLLTWQELE KKVCGDPEVT
760 770 780 790 800
VDALRKLTRF EDFEPSDTRV QYFWEALNNF TNEDRSRFLR FVTGRSRLPA
810 820 830 840 850
RIYIYPDKLG YETTDALPES STCSSTLFLP HYASAKVCEE KLRYAAYNCV
860
AIDTDMSPWE E
Length:861
Mass (Da):97,347
Last modified:June 27, 2006 - v2
Checksum:i305112B035FB9922
GO
Isoform 2 (identifier: Q3U487-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-651: Missing.

Show »
Length:210
Mass (Da):24,148
Checksum:i93D98CD167B13274
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti549A → V in BAE32546 (PubMed:16141072).Curated1
Sequence conflicti732D → G in BAE38213 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0194411 – 651Missing in isoform 2. 1 PublicationAdd BLAST651

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051385 mRNA Translation: BAC34620.1
AK139305 mRNA Translation: BAE23948.1
AK154378 mRNA Translation: BAE32546.1
AK155290 mRNA Translation: BAE33170.1
AK155676 mRNA Translation: BAE33384.1
AK163323 mRNA Translation: BAE37300.1
AK165482 mRNA Translation: BAE38213.1
AK170930 mRNA Translation: BAE42122.1
AL671671 Genomic DNA No translation available.
BC024415 mRNA Translation: AAH24415.1
BC070411 mRNA Translation: AAH70411.1
BC082276 mRNA Translation: AAH82276.1
CCDSiCCDS18522.1 [Q3U487-1]
RefSeqiNP_780453.1, NM_175244.3 [Q3U487-1]
UniGeneiMm.251420

Genome annotation databases

EnsembliENSMUST00000050067; ENSMUSP00000051922; ENSMUSG00000046861 [Q3U487-1]
GeneIDi76608
KEGGimmu:76608
UCSCiuc008uht.1 mouse [Q3U487-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHECD3_MOUSE
AccessioniPrimary (citable) accession number: Q3U487
Secondary accession number(s): B1AUL1
, Q3TN76, Q641P3, Q8BQ74, Q8R1L6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: May 23, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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