UniProtKB - Q3U2S8 (HVCN1_MOUSE)
Protein
Voltage-gated hydrogen channel 1
Gene
Hvcn1
Organism
Mus musculus (Mouse)
Status
Functioni
Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.5 Publications
Activity regulationi
The dimers display cooperative channel gating. The channel activity is inhibited by zinc ions.4 Publications
GO - Molecular functioni
- identical protein binding Source: IntAct
- voltage-gated cation channel activity Source: MGI
- voltage-gated proton channel activity Source: HGNC-UCL
GO - Biological processi
- cellular response to pH Source: UniProtKB
- cellular response to zinc ion Source: UniProtKB
- positive regulation of superoxide anion generation Source: MGI
- proton transmembrane transport Source: HGNC-UCL
- regulation of intracellular pH Source: MGI
- regulation of ion transmembrane transport Source: UniProtKB-KW
- response to pH Source: HGNC-UCL
- response to zinc ion Source: HGNC-UCL
Keywordsi
Molecular function | Ion channel, Voltage-gated channel |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-1222556, ROS and RNS production in phagocytes R-MMU-1300642, Sperm Motility And Taxes R-MMU-6798695, Neutrophil degranulation |
Protein family/group databases
TCDBi | 1.A.51.1.1, the voltage-gated proton channel (vpc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Voltage-gated hydrogen channel 1Alternative name(s): Hydrogen voltage-gated channel 1 Short name: HV1 Voltage sensor domain-only protein Short name: mVSOP |
Gene namesi | Name:Hvcn1 Synonyms:Bts, Vsop |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921346, Hvcn1 |
Subcellular locationi
Plasma membrane
Other locations
Plasma Membrane
- apical plasma membrane Source: MGI
- integral component of plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 96 | CytoplasmicSequence analysisAdd BLAST | 96 | |
Transmembranei | 97 – 117 | Helical; Name=Segment S1Add BLAST | 21 | |
Topological domaini | 118 – 134 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 135 – 157 | Helical; Name=Segment S2Add BLAST | 23 | |
Topological domaini | 158 – 165 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 166 – 186 | Helical; Name=Segment S3Add BLAST | 21 | |
Topological domaini | 187 – 193 | ExtracellularSequence analysis | 7 | |
Transmembranei | 194 – 214 | Helical; Name=Segment S4Add BLAST | 21 | |
Topological domaini | 215 – 269 | CytoplasmicSequence analysisAdd BLAST | 55 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 201 | R → Q: Faster channel activation kinetics. 1 Publication | 1 | |
Mutagenesisi | 207 | R → Q: Same activation kinetics as wild-type. 1 Publication | 1 | |
Mutagenesisi | 216 – 218 | VKT → GGG: Impairs gating cooperativity. 1 Publication | 3 | |
Mutagenesisi | 230 – 232 | INI → NIN: Slows channel activation. 1 Publication | 3 | |
Mutagenesisi | 231 | N → L: No effect on channel activation kinetics. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000342188 | 1 – 269 | Voltage-gated hydrogen channel 1Add BLAST | 269 |
Post-translational modificationi
Phosphorylation may enhance channel gating.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q3U2S8 |
MaxQBi | Q3U2S8 |
PaxDbi | Q3U2S8 |
PRIDEi | Q3U2S8 |
ProteomicsDBi | 273325 |
TopDownProteomicsi | Q3U2S8 |
PTM databases
iPTMneti | Q3U2S8 |
PhosphoSitePlusi | Q3U2S8 |
Expressioni
Tissue specificityi
Enriched in immune tissues, such as bone marrow, macrophages and spleen.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000064267, Expressed in lymph node and 150 other tissues |
Genevisiblei | Q3U2S8, MM |
Interactioni
Subunit structurei
Homodimer.
3 PublicationsBinary interactionsi
Q3U2S8
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-8401579,EBI-8401579 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 216489, 1 interactor |
DIPi | DIP-46134N |
IntActi | Q3U2S8, 1 interactor |
MINTi | Q3U2S8 |
STRINGi | 10090.ENSMUSP00000072401 |
Miscellaneous databases
RNActi | Q3U2S8, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q3U2S8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 221 – 261 | 2 PublicationsAdd BLAST | 41 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 46 – 53 | Poly-Glu | 8 | |
Compositional biasi | 173 – 176 | Poly-Val | 4 |
Domaini
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Unlike other voltage-gated ion channels it lacks the pore domain.1 Publication
The C-terminal coiled coil region mediates homodimerization and cooperative channel gating. It is essential for normal subcellular localization.1 Publication
Sequence similaritiesi
Belongs to the hydrogen channel family.Curated
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RX8B, Eukaryota |
GeneTreei | ENSGT00940000159403 |
HOGENOMi | CLU_076372_0_0_1 |
InParanoidi | Q3U2S8 |
OMAi | KVFHYLS |
OrthoDBi | 1601156at2759 |
PhylomeDBi | Q3U2S8 |
TreeFami | TF332056 |
Family and domain databases
Gene3Di | 1.20.120.350, 1 hit |
InterProi | View protein in InterPro IPR031846, Hvcn1 IPR005821, Ion_trans_dom IPR031844, VGPC1_C IPR027359, Volt_channel_dom_sf |
PANTHERi | PTHR46480, PTHR46480, 1 hit |
Pfami | View protein in Pfam PF00520, Ion_trans, 1 hit PF16799, VGPC1_C, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q3U2S8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSHDPKAVT RRTKVAPTKR MSRFLKHFTV VGDDYHTWNV NYKKWENEEE
60 70 80 90 100
EEEPAPTSAE GEGNAEGPDA EAGSASTPRQ SLDFRSRLRK LFSSHRFQVI
110 120 130 140 150
IICLVVLDAL LVLAELLLDL KIIEPDEQDY AVTAFHYMSF AILVFFMLEI
160 170 180 190 200
FFKIFVFRLE FFHHKFEILD AFVVVVSFVL DLVLLFKSHH FEALGLLILL
210 220 230 240 250
RLWRVARIIN GIIISVKTRS ERQILRLKQI NIQLATKIQH LEFSCSEKEQ
260
EIERLNKLLK QNGLLGDVN
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z019 | D3Z019_MOUSE | Voltage-gated hydrogen channel 1 | Hvcn1 | 168 | Annotation score: | ||
A0A0G2JGA0 | A0A0G2JGA0_MOUSE | Voltage-gated hydrogen channel 1 | Hvcn1 | 41 | Annotation score: | ||
D3YZ46 | D3YZ46_MOUSE | Voltage-gated hydrogen channel 1 | Hvcn1 | 47 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 66 | E → A in BAE33062 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002854 mRNA Translation: BAB22409.1 AK154880 mRNA Translation: BAE32899.1 AK155123 mRNA Translation: BAE33062.1 AK170422 mRNA Translation: BAE41787.1 BC021548 mRNA Translation: AAH21548.1 |
CCDSi | CCDS19644.1 |
RefSeqi | NP_001035954.1, NM_001042489.2 NP_083028.1, NM_028752.3 XP_006530535.1, XM_006530472.2 XP_006530536.1, XM_006530473.1 |
Genome annotation databases
Ensembli | ENSMUST00000072602; ENSMUSP00000072401; ENSMUSG00000064267 ENSMUST00000100747; ENSMUSP00000098312; ENSMUSG00000064267 |
GeneIDi | 74096 |
KEGGi | mmu:74096 |
UCSCi | uc008zku.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002854 mRNA Translation: BAB22409.1 AK154880 mRNA Translation: BAE32899.1 AK155123 mRNA Translation: BAE33062.1 AK170422 mRNA Translation: BAE41787.1 BC021548 mRNA Translation: AAH21548.1 |
CCDSi | CCDS19644.1 |
RefSeqi | NP_001035954.1, NM_001042489.2 NP_083028.1, NM_028752.3 XP_006530535.1, XM_006530472.2 XP_006530536.1, XM_006530473.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3VMX | X-ray | 1.45 | A/B/C/D | 220-266 | [»] | |
3VMY | X-ray | 1.47 | A/B/C/D | 220-269 | [»] | |
3VMZ | X-ray | 1.55 | A/B/C/D | 220-269 | [»] | |
3VN0 | X-ray | 1.37 | A/B/C/D | 220-269 | [»] | |
3VYI | X-ray | 2.30 | A/B/C/D/E/F/G/H/I/J/K/L | 220-269 | [»] | |
3WKV | X-ray | 3.45 | A | 73-215 | [»] | |
SMRi | Q3U2S8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 216489, 1 interactor |
DIPi | DIP-46134N |
IntActi | Q3U2S8, 1 interactor |
MINTi | Q3U2S8 |
STRINGi | 10090.ENSMUSP00000072401 |
Protein family/group databases
TCDBi | 1.A.51.1.1, the voltage-gated proton channel (vpc) family |
PTM databases
iPTMneti | Q3U2S8 |
PhosphoSitePlusi | Q3U2S8 |
Proteomic databases
EPDi | Q3U2S8 |
MaxQBi | Q3U2S8 |
PaxDbi | Q3U2S8 |
PRIDEi | Q3U2S8 |
ProteomicsDBi | 273325 |
TopDownProteomicsi | Q3U2S8 |
Protocols and materials databases
Antibodypediai | 31051, 157 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000072602; ENSMUSP00000072401; ENSMUSG00000064267 ENSMUST00000100747; ENSMUSP00000098312; ENSMUSG00000064267 |
GeneIDi | 74096 |
KEGGi | mmu:74096 |
UCSCi | uc008zku.2, mouse |
Organism-specific databases
CTDi | 84329 |
MGIi | MGI:1921346, Hvcn1 |
Phylogenomic databases
eggNOGi | ENOG502RX8B, Eukaryota |
GeneTreei | ENSGT00940000159403 |
HOGENOMi | CLU_076372_0_0_1 |
InParanoidi | Q3U2S8 |
OMAi | KVFHYLS |
OrthoDBi | 1601156at2759 |
PhylomeDBi | Q3U2S8 |
TreeFami | TF332056 |
Enzyme and pathway databases
Reactomei | R-MMU-1222556, ROS and RNS production in phagocytes R-MMU-1300642, Sperm Motility And Taxes R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 74096, 1 hit in 52 CRISPR screens |
ChiTaRSi | Hvcn1, mouse |
PROi | PR:Q3U2S8 |
RNActi | Q3U2S8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000064267, Expressed in lymph node and 150 other tissues |
Genevisiblei | Q3U2S8, MM |
Family and domain databases
Gene3Di | 1.20.120.350, 1 hit |
InterProi | View protein in InterPro IPR031846, Hvcn1 IPR005821, Ion_trans_dom IPR031844, VGPC1_C IPR027359, Volt_channel_dom_sf |
PANTHERi | PTHR46480, PTHR46480, 1 hit |
Pfami | View protein in Pfam PF00520, Ion_trans, 1 hit PF16799, VGPC1_C, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HVCN1_MOUSE | |
Accessioni | Q3U2S8Primary (citable) accession number: Q3U2S8 Secondary accession number(s): Q9DCE4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 2008 |
Last sequence update: | July 1, 2008 | |
Last modified: | April 7, 2021 | |
This is version 120 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families