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Protein

Valine--tRNA ligase, mitochondrial

Gene

Vars2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei662ATPBy similarity1

GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • valine-tRNA ligase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligase, mitochondrial (EC:6.1.1.9)
Alternative name(s):
Valyl-tRNA synthetase
Short name:
ValRS
Gene namesi
Name:Vars2
Synonyms:Kiaa1885, Vars2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1916165 Vars2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 15MitochondrionSequence analysisAdd BLAST15
ChainiPRO_000033800216 – 1060Valine--tRNA ligase, mitochondrialAdd BLAST1045

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei548N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ3U2A8
MaxQBiQ3U2A8
PaxDbiQ3U2A8
PeptideAtlasiQ3U2A8
PRIDEiQ3U2A8

PTM databases

iPTMnetiQ3U2A8
PhosphoSitePlusiQ3U2A8

Expressioni

Gene expression databases

BgeeiENSMUSG00000038838 Expressed in 205 organ(s), highest expression level in thymus
CleanExiMM_VARS2
ExpressionAtlasiQ3U2A8 baseline and differential
GenevisibleiQ3U2A8 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047917

Structurei

3D structure databases

ProteinModelPortaliQ3U2A8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi146 – 156"HIGH" regionAdd BLAST11
Motifi659 – 663"KMSKS" region5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0432 Eukaryota
COG0525 LUCA
GeneTreeiENSGT00550000074727
HOGENOMiHOG000020094
HOVERGENiHBG017878
InParanoidiQ3U2A8
KOiK01873
OMAiRQWYIRN
OrthoDBiEOG091G01XH
TreeFamiTF354250

Family and domain databases

CDDicd07962 Anticodon_Ia_Val, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 2 hits
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033705 Anticodon_Ia_Val
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR002303 Valyl-tRNA_ligase
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00986 TRNASYNTHVAL
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00422 valS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q3U2A8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPHLPLASFR PPLWGLRPSW GLSRPQALCT QPEPHGSPVS RRNREAKQKR
60 70 80 90 100
LREKQAALEA GLAEKSKIPA VPTKAWSHKE VVLYEIPTGP GEKKDVSGPL
110 120 130 140 150
PPAYSPQYVE AAWYQWWVRE GFFKPEYQAR LPQATGETFS MCIPPPNVTG
160 170 180 190 200
SLHIGHALTV AIQDALVRWH RMRGDRVLWI PGSDHAGIAT QAMVEKQLWK
210 220 230 240 250
EQRVRRHELS REDFLRAVWQ WKHEKGGEIY EQLCALGASL DWDRECFTMD
260 270 280 290 300
AGSSAAVTEA FVRLYNSGLL YRNRQLVNWS CTLRSAISDI EVESRPLPGR
310 320 330 340 350
TVLQLPGCPT PVSFGLLASV AFPVDGEPDT EIVVGTTRPE TLPGDVAVAV
360 370 380 390 400
HPDDPRYTHL HGRQLRHPLT GQLLPLITDT TVQPHVGTGA VKVTPAHSPI
410 420 430 440 450
DAEIGTRHGL TPLSVIAEDG TMTSLCGDWL QGLHRFVARE KIMCTLREQG
460 470 480 490 500
LFRGLQEHPM VLPICSRSGD VVEYLLKSQW FVRCQEMGDL AAKAVESGAL
510 520 530 540 550
ELWPSFHQKS WQHWFAHIGD WCVSRQLWWG HQIPAYRVIG ENAEDDRKEC
560 570 580 590 600
WVVGRSEAEA RAVAAKRTGR PEAELTLERD PDVLDTWFSS ALFPFSALGW
610 620 630 640 650
PRETPDLAHF YPLTLLETGS DLLMFWVGRM VMLGTQLTGQ LPFSKVLLHS
660 670 680 690 700
MVRDRQGRKM SKSLGNVLDP RDIISGQELQ VLQAKLRDGN LDPGELAVAA
710 720 730 740 750
AAQKKDFPYG IPECGTDALR FALCSHGILG GDLHLSVSEV LNYRHFCNKL
760 770 780 790 800
WNALRFVLRA LGDNFVPQPA EEVTPSSPMD AWILSRLAFA ASECERGFLS
810 820 830 840 850
RELSLVTHTL YHFWLHNLCD VYLEAVKPVL SSVPRPPGPP QVLFSCADVG
860 870 880 890 900
LRLLAPLMPF LAEELWQRLP PRPGGPLAPS ICVAPYPSTR SLEFWRQPEL
910 920 930 940 950
ERCFSRVQEV VQALRALRAT YQLTKARPQV LLQCSDPGEQ GLVQPFLEPL
960 970 980 990 1000
GILSHCGAVG FLSPGAAAPS GWALTPLGDT MKIYMELQGL VDPQSQLPRL
1010 1020 1030 1040 1050
TARRQKLQKQ LDDLLNRTMS EGLAERQQRI SSLHLELSKL DQAASYLQQL
1060
MDEAPSAREL
Length:1,060
Mass (Da):118,461
Last modified:July 27, 2011 - v2
Checksum:iC0CBFF593F54E657
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6YJY2F6YJY2_MOUSE
Valine--tRNA ligase, mitochondrial
Vars2
581Annotation score:
F7A8L1F7A8L1_MOUSE
Valine--tRNA ligase, mitochondrial
Vars2
215Annotation score:
F6Z939F6Z939_MOUSE
Valine--tRNA ligase, mitochondrial
Vars2
112Annotation score:
E9PWQ5E9PWQ5_MOUSE
Valine--tRNA ligase, mitochondrial
Vars2
75Annotation score:

Sequence cautioni

The sequence BAC29932 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD32566 differs from that shown. The sequence differs from that shown because it seems to be derived from a pre-mRNA.Curated
The sequence BAD32566 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57A → V in BAD32566 (PubMed:15368895).Curated1
Sequence conflicti57A → V in BAC87668 (Ref. 3) Curated1
Sequence conflicti193M → V in BAD32566 (PubMed:15368895).Curated1
Sequence conflicti193M → V in BAC87668 (Ref. 3) Curated1
Sequence conflicti193M → V in BAE33234 (Ref. 3) Curated1
Sequence conflicti327E → L in BAD32566 (PubMed:15368895).Curated1
Sequence conflicti327E → L in BAC87668 (Ref. 3) Curated1
Sequence conflicti366R → H in BAD32566 (PubMed:15368895).Curated1
Sequence conflicti366R → H in BAC87668 (Ref. 3) Curated1
Sequence conflicti383Q → R in BAC87668 (Ref. 3) Curated1
Sequence conflicti404I → M in BAD32566 (PubMed:15368895).Curated1
Sequence conflicti404I → M in BAC87668 (Ref. 3) Curated1
Sequence conflicti408H → R in BAC87668 (Ref. 3) Curated1
Sequence conflicti449Q → R in BAD32566 (PubMed:15368895).Curated1
Sequence conflicti449Q → R in BAC87668 (Ref. 3) Curated1
Sequence conflicti490L → R in BAD32566 (PubMed:15368895).Curated1
Sequence conflicti490L → R in BAC87668 (Ref. 3) Curated1
Sequence conflicti490L → R in BAE33234 (Ref. 3) Curated1
Sequence conflicti609H → R in BAD32566 (PubMed:15368895).Curated1
Sequence conflicti609H → R in BAC87668 (Ref. 3) Curated1
Sequence conflicti889T → A in BAD32566 (PubMed:15368895).Curated1
Sequence conflicti889T → A in BAC87668 (Ref. 3) Curated1
Sequence conflicti889T → A in BAE33234 (Ref. 3) Curated1
Sequence conflicti896R → H in BAC87668 (Ref. 3) Curated1
Sequence conflicti944Missing in BAC87668 (Ref. 3) Curated1
Sequence conflicti975T → A in BAD32566 (PubMed:15368895).Curated1
Sequence conflicti975T → A in BAC87668 (Ref. 3) Curated1
Sequence conflicti975T → A in BAE33234 (Ref. 3) Curated1
Sequence conflicti1019M → V in BAE33234 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173288 Transcribed RNA Translation: BAD32566.1 Sequence problems.
AK038117 mRNA Translation: BAC29932.1 Different initiation.
AK155386 mRNA Translation: BAE33234.1
AK129008 mRNA Translation: BAC87668.1
CR974483 Genomic DNA No translation available.
BC057036 mRNA Translation: AAH57036.2
CCDSiCCDS37602.1
RefSeqiNP_780346.3, NM_175137.4
UniGeneiMm.32002

Genome annotation databases

EnsembliENSMUST00000043674; ENSMUSP00000047917; ENSMUSG00000038838
GeneIDi68915
KEGGimmu:68915
UCSCiuc008cie.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173288 Transcribed RNA Translation: BAD32566.1 Sequence problems.
AK038117 mRNA Translation: BAC29932.1 Different initiation.
AK155386 mRNA Translation: BAE33234.1
AK129008 mRNA Translation: BAC87668.1
CR974483 Genomic DNA No translation available.
BC057036 mRNA Translation: AAH57036.2
CCDSiCCDS37602.1
RefSeqiNP_780346.3, NM_175137.4
UniGeneiMm.32002

3D structure databases

ProteinModelPortaliQ3U2A8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047917

PTM databases

iPTMnetiQ3U2A8
PhosphoSitePlusiQ3U2A8

Proteomic databases

EPDiQ3U2A8
MaxQBiQ3U2A8
PaxDbiQ3U2A8
PeptideAtlasiQ3U2A8
PRIDEiQ3U2A8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043674; ENSMUSP00000047917; ENSMUSG00000038838
GeneIDi68915
KEGGimmu:68915
UCSCiuc008cie.2 mouse

Organism-specific databases

CTDi57176
MGIiMGI:1916165 Vars2
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0432 Eukaryota
COG0525 LUCA
GeneTreeiENSGT00550000074727
HOGENOMiHOG000020094
HOVERGENiHBG017878
InParanoidiQ3U2A8
KOiK01873
OMAiRQWYIRN
OrthoDBiEOG091G01XH
TreeFamiTF354250

Miscellaneous databases

PROiPR:Q3U2A8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038838 Expressed in 205 organ(s), highest expression level in thymus
CleanExiMM_VARS2
ExpressionAtlasiQ3U2A8 baseline and differential
GenevisibleiQ3U2A8 MM

Family and domain databases

CDDicd07962 Anticodon_Ia_Val, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 2 hits
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033705 Anticodon_Ia_Val
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR002303 Valyl-tRNA_ligase
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00986 TRNASYNTHVAL
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00422 valS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSYVM_MOUSE
AccessioniPrimary (citable) accession number: Q3U2A8
Secondary accession number(s): E9QMJ4
, Q69Z78, Q6PGG3, Q6ZQL7, Q8BIN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
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