UniProtKB - Q3U1V8 (M3K9_MOUSE)
Mitogen-activated protein kinase kinase kinase 9
Map3k9
Functioni
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis (By similarity).
By similarityCatalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 164 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 261 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 143 – 151 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- JUN kinase kinase kinase activity Source: GO_Central
- protein kinase activity Source: GO_Central
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: MGI
- protein serine kinase activity Source: RHEA
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell death Source: MGI
- positive regulation of apoptotic process Source: MGI
- protein autophosphorylation Source: MGI
- protein phosphorylation Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Apoptosis, Stress response, Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Map3k9 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2449952, Map3k9 |
VEuPathDBi | HostDB:ENSMUSG00000042724 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000277825 | 1 – 1077 | Mitogen-activated protein kinase kinase kinase 9Add BLAST | 1077 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 297 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 298 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 301 | Phosphoserine; by autocatalysisBy similarity | 1 | |
Modified residuei | 305 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 526 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q3U1V8 |
PaxDbi | Q3U1V8 |
PeptideAtlasi | Q3U1V8 |
PRIDEi | Q3U1V8 |
ProteomicsDBi | 252701 |
PTM databases
iPTMneti | Q3U1V8 |
PhosphoSitePlusi | Q3U1V8 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000042724, Expressed in brain and 178 other tissues |
ExpressionAtlasi | Q3U1V8, baseline and differential |
Genevisiblei | Q3U1V8, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 237224, 1 interactor |
IntActi | Q3U1V8, 2 interactors |
STRINGi | 10090.ENSMUSP00000041819 |
Miscellaneous databases
RNActi | Q3U1V8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 109 | SH3PROSITE-ProRule annotationAdd BLAST | 65 | |
Domaini | 137 – 405 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 269 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 40 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 423 – 444 | Leucine-zipper 1Add BLAST | 22 | |
Regioni | 458 – 479 | Leucine-zipper 2Add BLAST | 22 | |
Regioni | 491 – 511 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 526 – 606 | DisorderedSequence analysisAdd BLAST | 81 | |
Regioni | 646 – 713 | DisorderedSequence analysisAdd BLAST | 68 | |
Regioni | 748 – 790 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 860 – 971 | DisorderedSequence analysisAdd BLAST | 112 | |
Regioni | 986 – 1011 | DisorderedSequence analysisAdd BLAST | 26 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 526 – 545 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 571 – 588 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 689 – 710 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 753 – 771 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 864 – 884 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 900 – 920 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 928 – 942 | Pro residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 988 – 1011 | Polar residuesSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
Repeat, SH3 domainPhylogenomic databases
eggNOGi | KOG0192, Eukaryota |
GeneTreei | ENSGT00940000158243 |
HOGENOMi | CLU_000288_7_14_1 |
InParanoidi | Q3U1V8 |
OrthoDBi | 115270at2759 |
PhylomeDBi | Q3U1V8 |
TreeFami | TF105118 |
Family and domain databases
CDDi | cd12059, SH3_MLK1-3, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR035779, MLK1-3_SH3 IPR016231, MLK1-4 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008271, Ser/Thr_kinase_AS IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF14604, SH3_9, 1 hit |
PIRSFi | PIRSF000556, MAPKKK9_11, 1 hit |
PRINTSi | PR00452, SH3DOMAIN PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50002, SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MESSRSLLGC LASATAAPPG DDATGAGAEE EEDEEEAAAE LGSHAALPYW
60 70 80 90 100
TAVFEYEAAG EDELTLRLGD VVEVLSKDSQ VSGDEGWWTG QLNQRVGIFP
110 120 130 140 150
SNYVTPRSAF SSRCQPGAED PSCYPPIQLL EIDFAELTLE EIIGIGGFGK
160 170 180 190 200
VYRAFWAGDE VAVKAARHDP DEDISQTIEN VRQEAKLFAM LKHPNIIALR
210 220 230 240 250
GVCLKEPNLC LVMEFARGGP LNRVLSGKRI PPDILVNWAV QIARGMNYLH
260 270 280 290 300
DEAIVPIIHR DLKSSNILIL QKVENGDLSN KILKITDFGL AREWHRTTKM
310 320 330 340 350
SAAGTYAWMA PEVIRASMFS KGSDVWSYGV LLWELLTGEV PFRGIDGLAV
360 370 380 390 400
AYGVAMNKLA LPIPSTCPEP FAKLMEDCWN PDPHSRPSFT SILDQLTTIE
410 420 430 440 450
ESGFFEMPKD SFHCLQDDWK HEIQEMFDQL RAKEKELRTW EEELTRAALQ
460 470 480 490 500
QKNQEELLRR REQELAEREI DILERELNII IHQLCQEKPR VKKRKGKFRK
510 520 530 540 550
SRLKLKDGNR ISLPSDFQHK FTVQASPTMD KRKSLISNRS SPPASPTIIP
560 570 580 590 600
RLRAIQLTPG ESSKTWGRSS VVPKEEGEEE EKRAPKKKGR TWGPGTLGQK
610 620 630 640 650
ELTSGDEGLK SLVDGYKQWS SSAPNLGKGP RSSPALPGFT SLMEIEDEDS
660 670 680 690 700
EGPGSGENHQ QHSPNQSYLC IPFPRGEDGD GPSSDGVHEE PTPVNSATST
710 720 730 740 750
PQLTPTNSLK RGGTHHRRCE VALLGCGAVL AATGLGFDLL EAGKCQLLPP
760 770 780 790 800
EEPEPPAREE KKRREGLFQR ASRPRRSTSP PSRKLFKKEE PMTLLGDPSA
810 820 830 840 850
SLTLLSLSSI SECNSTRSLL RSDSDEIVVY EMPVSPVEAP PLTQCTHNPL
860 870 880 890 900
VNVRVERFKR DPNQSLTPTH VTLTAPTQPS GHRRTPSDGA LKPTAAPAVL
910 920 930 940 950
GSRSPSSNGM SPSPGTGMLK TPSPSRDPGE FPRLPDPNVV FPPTPRRWNT
960 970 980 990 1000
QRDSTLERPK TLEFLPRPRP SANRQRLDPW WFVSPSHARS ASPANSSSTE
1010 1020 1030 1040 1050
TPSNLDSCFA SSSSTVEERP GLPALLPLQA GPLLPAERTL LDLDAEGQSQ
1060 1070
DSTVPLCRAE LNAHGPSPYE IQQEFWS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1Y7VKX4 | A0A1Y7VKX4_MOUSE | Mitogen-activated protein kinase ki... | Map3k9 | 1,100 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 176 | Q → K in BAC35552 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 284 | K → E in BAE33385 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK053843 mRNA Translation: BAC35552.1 AK155677 mRNA Translation: BAE33385.1 AC124595 Genomic DNA No translation available. AC125351 Genomic DNA No translation available. |
CCDSi | CCDS49104.1 |
RefSeqi | NP_001167578.1, NM_001174107.1 NP_796369.2, NM_177395.5 |
Genome annotation databases
Ensembli | ENSMUST00000035987; ENSMUSP00000041819; ENSMUSG00000042724 |
GeneIDi | 338372 |
KEGGi | mmu:338372 |
UCSCi | uc007oco.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK053843 mRNA Translation: BAC35552.1 AK155677 mRNA Translation: BAE33385.1 AC124595 Genomic DNA No translation available. AC125351 Genomic DNA No translation available. |
CCDSi | CCDS49104.1 |
RefSeqi | NP_001167578.1, NM_001174107.1 NP_796369.2, NM_177395.5 |
3D structure databases
SMRi | Q3U1V8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 237224, 1 interactor |
IntActi | Q3U1V8, 2 interactors |
STRINGi | 10090.ENSMUSP00000041819 |
PTM databases
iPTMneti | Q3U1V8 |
PhosphoSitePlusi | Q3U1V8 |
Proteomic databases
MaxQBi | Q3U1V8 |
PaxDbi | Q3U1V8 |
PeptideAtlasi | Q3U1V8 |
PRIDEi | Q3U1V8 |
ProteomicsDBi | 252701 |
Protocols and materials databases
Antibodypediai | 75, 365 antibodies from 32 providers |
DNASUi | 338372 |
Genome annotation databases
Ensembli | ENSMUST00000035987; ENSMUSP00000041819; ENSMUSG00000042724 |
GeneIDi | 338372 |
KEGGi | mmu:338372 |
UCSCi | uc007oco.2, mouse |
Organism-specific databases
CTDi | 4293 |
MGIi | MGI:2449952, Map3k9 |
VEuPathDBi | HostDB:ENSMUSG00000042724 |
Phylogenomic databases
eggNOGi | KOG0192, Eukaryota |
GeneTreei | ENSGT00940000158243 |
HOGENOMi | CLU_000288_7_14_1 |
InParanoidi | Q3U1V8 |
OrthoDBi | 115270at2759 |
PhylomeDBi | Q3U1V8 |
TreeFami | TF105118 |
Miscellaneous databases
BioGRID-ORCSi | 338372, 0 hits in 65 CRISPR screens |
PROi | PR:Q3U1V8 |
RNActi | Q3U1V8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000042724, Expressed in brain and 178 other tissues |
ExpressionAtlasi | Q3U1V8, baseline and differential |
Genevisiblei | Q3U1V8, MM |
Family and domain databases
CDDi | cd12059, SH3_MLK1-3, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR035779, MLK1-3_SH3 IPR016231, MLK1-4 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008271, Ser/Thr_kinase_AS IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF14604, SH3_9, 1 hit |
PIRSFi | PIRSF000556, MAPKKK9_11, 1 hit |
PRINTSi | PR00452, SH3DOMAIN PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | M3K9_MOUSE | |
Accessioni | Q3U1V8Primary (citable) accession number: Q3U1V8 Secondary accession number(s): E9QLZ4, Q8BIG8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families