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Protein

Phosphoprotein associated with glycosphingolipid-enriched microdomains 1

Gene

Pag1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-180292 GAB1 signalosome
R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Alternative name(s):
Csk-binding protein
Transmembrane phosphoprotein Cbp
Gene namesi
Name:Pag1
Synonyms:Cbp, Pag
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2443160 Pag1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17ExtracellularSequence analysisAdd BLAST17
Transmembranei18 – 38Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini39 – 429CytoplasmicSequence analysisAdd BLAST391

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable and do not show any developmental defect up to 20 months of age. They have normal T-cell development and normal T- and B-cell responses.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi314Y → F: Enhances TCR-mediated signaling. 1 Publication1
Mutagenesisi429L → A: Abolishes interaction with SLC9A3R1 and effect on synapse formation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833391 – 429Phosphoprotein associated with glycosphingolipid-enriched microdomains 1Add BLAST429

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi39S-palmitoyl cysteineBy similarity1
Lipidationi42S-palmitoyl cysteineBy similarity1
Modified residuei52PhosphoserineBy similarity1
Modified residuei63PhosphoserineBy similarity1
Modified residuei107Phosphotyrosine; by LYNBy similarity1
Modified residuei165PhosphotyrosineCombined sources1
Modified residuei183PhosphotyrosineCombined sources1
Modified residuei224PhosphotyrosineCombined sources1
Modified residuei226PhosphoserineBy similarity1
Modified residuei314Phosphotyrosine; by FYN3 Publications1
Modified residuei351PhosphoserineCombined sources1
Modified residuei356PhosphotyrosineBy similarity1
Modified residuei379PhosphoserineCombined sources1
Modified residuei386PhosphotyrosineBy similarity1
Modified residuei414PhosphotyrosineBy similarity1

Post-translational modificationi

Palmitoylated.1 Publication
Phosphorylated by FYN on Tyr-314 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.5 Publications

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ3U1F9
MaxQBiQ3U1F9
PaxDbiQ3U1F9
PRIDEiQ3U1F9

PTM databases

iPTMnetiQ3U1F9
PhosphoSitePlusiQ3U1F9
SwissPalmiQ3U1F9

Expressioni

Tissue specificityi

Present in T-cells (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027508 Expressed in 282 organ(s), highest expression level in pineal body
ExpressionAtlasiQ3U1F9 baseline and differential
GenevisibleiQ3U1F9 MM

Interactioni

Subunit structurei

When phosphorylated, interacts with CSK. Interacts with SLC9A3R1/EBP50. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CskP412419EBI-8468834,EBI-2553183

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220469, 3 interactors
IntActiQ3U1F9, 119 interactors
MINTiQ3U1F9
STRINGi10090.ENSMUSP00000104021

Structurei

3D structure databases

ProteinModelPortaliQ3U1F9
SMRiQ3U1F9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni314 – 317Interaction with CSK4
Regioni427 – 429Interaction with SLC9A3R11 Publication3

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFZ3 Eukaryota
ENOG4111TW0 LUCA
GeneTreeiENSGT00390000002061
HOGENOMiHOG000290651
HOVERGENiHBG055052
InParanoidiQ3U1F9
OMAiPKCHQSR
OrthoDBiEOG091G09DV
PhylomeDBiQ3U1F9
TreeFamiTF336170

Family and domain databases

InterProiView protein in InterPro
IPR032748 PAG
PANTHERiPTHR16322 PTHR16322, 1 hit
PfamiView protein in Pfam
PF15347 PAG, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD340439 PD340439, 1 hit

Sequencei

Sequence statusi: Complete.

Q3U1F9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGPAGSVLSS GQMQMQMVLW GSLAAVAMFF LITFLVLLCS TCDREKKPRQ
60 70 80 90 100
HSGDHENLMN VPSDKDMFSH SATSLTTDAL ASSEQNGVLT NGDILSEDST
110 120 130 140 150
LTCMQHYEEV QTSASDLLDS QDSTGKAKCH QSRELPRIPP ENAVDEILTA
160 170 180 190 200
RAADTELGPG VEGPYEVLKD SSSQENMVED CLYETVKEIK EVADKGQGGK
210 220 230 240 250
SKSTSALKEL QGAPMEGKAD FAEYASVDRN KKCRHSANAE SILGTCSDLD
260 270 280 290 300
EESPPPVPVK LLDENANLPQ EGGGQAEEQA AEGTGGHSKR FSSLSYKSRE
310 320 330 340 350
EDPTLTEEEI SAMYSSVNKP GQSAHKPGPC MKGPESACHS MKGLPQRSSS
360 370 380 390 400
SCNDLYATVK DFEKTPNSIS TLPPARRPSE EPEPDYEAIQ TLNREDEKVP
410 420
LETNGHHVPK ESDYESIGDL QQCRDVTRL
Length:429
Mass (Da):46,549
Last modified:December 20, 2005 - v2
Checksum:iF5A95257B4C4DADC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58L → P in AAG44565 (PubMed:10790433).Curated1
Sequence conflicti287H → R in AAG44565 (PubMed:10790433).Curated1
Sequence conflicti360K → R in BAE33539 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF250192 mRNA Translation: AAG44565.1
AK156000 mRNA Translation: BAE33539.1
AK040230 mRNA Translation: BAC30545.1
AK089329 mRNA Translation: BAC40842.1
AK172106 mRNA Translation: BAE42829.1
CCDSiCCDS17235.1
RefSeqiNP_001181960.1, NM_001195031.1
NP_444412.2, NM_053182.5
XP_006530170.1, XM_006530107.1
XP_011246453.1, XM_011248151.2
UniGeneiMm.23897
Mm.486503

Genome annotation databases

EnsembliENSMUST00000108384; ENSMUSP00000104021; ENSMUSG00000027508
ENSMUST00000161949; ENSMUSP00000124529; ENSMUSG00000027508
GeneIDi94212
KEGGimmu:94212
UCSCiuc008opc.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF250192 mRNA Translation: AAG44565.1
AK156000 mRNA Translation: BAE33539.1
AK040230 mRNA Translation: BAC30545.1
AK089329 mRNA Translation: BAC40842.1
AK172106 mRNA Translation: BAE42829.1
CCDSiCCDS17235.1
RefSeqiNP_001181960.1, NM_001195031.1
NP_444412.2, NM_053182.5
XP_006530170.1, XM_006530107.1
XP_011246453.1, XM_011248151.2
UniGeneiMm.23897
Mm.486503

3D structure databases

ProteinModelPortaliQ3U1F9
SMRiQ3U1F9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220469, 3 interactors
IntActiQ3U1F9, 119 interactors
MINTiQ3U1F9
STRINGi10090.ENSMUSP00000104021

PTM databases

iPTMnetiQ3U1F9
PhosphoSitePlusiQ3U1F9
SwissPalmiQ3U1F9

Proteomic databases

EPDiQ3U1F9
MaxQBiQ3U1F9
PaxDbiQ3U1F9
PRIDEiQ3U1F9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108384; ENSMUSP00000104021; ENSMUSG00000027508
ENSMUST00000161949; ENSMUSP00000124529; ENSMUSG00000027508
GeneIDi94212
KEGGimmu:94212
UCSCiuc008opc.2 mouse

Organism-specific databases

CTDi55824
MGIiMGI:2443160 Pag1

Phylogenomic databases

eggNOGiENOG410IFZ3 Eukaryota
ENOG4111TW0 LUCA
GeneTreeiENSGT00390000002061
HOGENOMiHOG000290651
HOVERGENiHBG055052
InParanoidiQ3U1F9
OMAiPKCHQSR
OrthoDBiEOG091G09DV
PhylomeDBiQ3U1F9
TreeFamiTF336170

Enzyme and pathway databases

ReactomeiR-MMU-180292 GAB1 signalosome
R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains

Miscellaneous databases

ChiTaRSiPag1 mouse
PROiPR:Q3U1F9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027508 Expressed in 282 organ(s), highest expression level in pineal body
ExpressionAtlasiQ3U1F9 baseline and differential
GenevisibleiQ3U1F9 MM

Family and domain databases

InterProiView protein in InterPro
IPR032748 PAG
PANTHERiPTHR16322 PTHR16322, 1 hit
PfamiView protein in Pfam
PF15347 PAG, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD340439 PD340439, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPHAG1_MOUSE
AccessioniPrimary (citable) accession number: Q3U1F9
Secondary accession number(s): Q8BFS1, Q9EQF3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 7, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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