UniProtKB - Q3U0V1 (FUBP2_MOUSE)
Protein
Far upstream element-binding protein 2
Gene
Khsrp
Organism
Mus musculus (Mouse)
Status
Functioni
Binds to the dendritic targeting element and may play a role in mRNA trafficking. Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs (By similarity).By similarity
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- mRNA 3'-UTR AU-rich region binding Source: UniProtKB
- mRNA 3'-UTR binding Source: MGI
- mRNA binding Source: MGI
GO - Biological processi
- 3'-UTR-mediated mRNA destabilization Source: UniProtKB
- cellular response to cytokine stimulus Source: UniProtKB
- miRNA metabolic process Source: UniProtKB
- mRNA catabolic process Source: MGI
- mRNA processing Source: UniProtKB-KW
- mRNA transport Source: UniProtKB-KW
- negative regulation of low-density lipoprotein particle clearance Source: MGI
- negative regulation of nitric oxide biosynthetic process Source: UniProtKB
- positive regulation of mRNA catabolic process Source: UniProtKB
- regulation of gene expression Source: GO_Central
- regulation of miRNA metabolic process Source: MGI
- regulation of mRNA stability Source: UniProtKB
- regulation of transcription, DNA-templated Source: InterPro
- RNA splicing Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, RNA-binding |
Biological process | mRNA processing, mRNA splicing, mRNA transport, Transcription, Transcription regulation, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-450604, KSRP (KHSRP) binds and destabilizes mRNA |
Names & Taxonomyi
Protein namesi | Recommended name: Far upstream element-binding protein 2Short name: FUSE-binding protein 2 Alternative name(s): KH type-splicing regulatory protein Short name: KSRP |
Gene namesi | Name:Khsrp Synonyms:Fubp2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1336214, Khsrp |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: GO_Central
- cytoplasmic stress granule Source: MGI
- dendrite Source: MGI
- neuronal cell body Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000298678 | 2 – 748 | Far upstream element-binding protein 2Add BLAST | 747 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 40 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 88 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 101 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 122 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 122 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 126 | PhosphoserineBy similarity | 1 | |
Modified residuei | 130 | PhosphoserineBy similarity | 1 | |
Modified residuei | 182 | PhosphoserineCombined sources | 1 | |
Modified residuei | 185 | PhosphoserineBy similarity | 1 | |
Modified residuei | 194 | PhosphoserineBy similarity | 1 | |
Modified residuei | 275 | PhosphoserineBy similarity | 1 | |
Modified residuei | 412 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 414 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 416 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 443 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 481 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q3U0V1 |
jPOSTi | Q3U0V1 |
MaxQBi | Q3U0V1 |
PaxDbi | Q3U0V1 |
PeptideAtlasi | Q3U0V1 |
PRIDEi | Q3U0V1 |
PTM databases
iPTMneti | Q3U0V1 |
PhosphoSitePlusi | Q3U0V1 |
SwissPalmi | Q3U0V1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000007670, Expressed in intestine and 254 other tissues |
ExpressionAtlasi | Q3U0V1, baseline and differential |
Genevisiblei | Q3U0V1, MM |
Interactioni
Subunit structurei
Part of a ternary complex containing FUBP2, PTBP1, PTBP2 and HNRPH1.
Interacts with PARN.
Interacts with PQBP1.
By similarityProtein-protein interaction databases
BioGRIDi | 200927, 21 interactors |
IntActi | Q3U0V1, 7 interactors |
STRINGi | 10090.ENSMUSP00000007814 |
Miscellaneous databases
RNActi | Q3U0V1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 145 – 209 | KH 1PROSITE-ProRule annotationAdd BLAST | 65 | |
Domaini | 234 – 300 | KH 2PROSITE-ProRule annotationAdd BLAST | 67 | |
Domaini | 323 – 387 | KH 3PROSITE-ProRule annotationAdd BLAST | 65 | |
Domaini | 425 – 492 | KH 4PROSITE-ProRule annotationAdd BLAST | 68 | |
Repeati | 572 – 583 | 1Add BLAST | 12 | |
Repeati | 618 – 629 | 2Add BLAST | 12 | |
Repeati | 644 – 655 | 3Add BLAST | 12 | |
Repeati | 674 – 685 | 4Add BLAST | 12 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 572 – 685 | 4 X 12 AA imperfect repeatsAdd BLAST | 114 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 7 – 68 | Gly/Pro-richAdd BLAST | 62 | |
Compositional biasi | 69 – 497 | Gly-richAdd BLAST | 429 | |
Compositional biasi | 499 – 613 | Ala/Gly/Pro-richAdd BLAST | 115 |
Sequence similaritiesi
Belongs to the KHSRP family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1676, Eukaryota |
GeneTreei | ENSGT00940000156051 |
HOGENOMi | CLU_014285_1_0_1 |
InParanoidi | Q3U0V1 |
OMAi | HIMGPPE |
OrthoDBi | 590738at2759 |
TreeFami | TF313654 |
Family and domain databases
Gene3Di | 3.30.1370.10, 4 hits |
InterProi | View protein in InterPro IPR015096, FUBP_C IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf |
Pfami | View protein in Pfam PF09005, DUF1897, 2 hits PF00013, KH_1, 4 hits |
SMARTi | View protein in SMART SM00322, KH, 4 hits |
SUPFAMi | SSF54791, SSF54791, 4 hits |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit PS50084, KH_TYPE_1, 4 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q3U0V1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDYNTGGPP PGPPPPAGGG GGAAGAGGGP PPGPPGAGDR GGGGPGGGGP
60 70 80 90 100
GGGGASGGPS QPPGGGGPGI RKDAFADAVQ RARQIAAKIG GDAATTVNNN
110 120 130 140 150
TPDFGFGGQK RQLEDGDQPD SKKLASQGDS IGSQLGPIHP PPRTSMTEEY
160 170 180 190 200
RVPDGMVGLI IGRGGEQINK IQQDSGCKVQ ISPDSGGLPE RSVSLTGAPE
210 220 230 240 250
SVQKAKMMLD DIVSRGRGGP PGQFHDNANG GQNGTVQEIM IPAGKAGLVI
260 270 280 290 300
GKGGETIKQL QERAGVKMIL IQDGSQNTNV DKPLRIIGDP YKVQQACEMV
310 320 330 340 350
MDILRERDQG GFGDRNEYGS RVGGGIDVPV PRHSVGVVIG RSGEMIKKIQ
360 370 380 390 400
NDAGVRIQFK QDDGTGPEKI AHIMGPPDRC EHAARIINDL LQSLRSGPPG
410 420 430 440 450
PPGAPGMPPG GRGRGRGQGN WGPPGGEMTF SIPTHKCGLV IGRGGENVKA
460 470 480 490 500
INQQTGAFVE ISRQLPPNGD PNFKLFVIRG SPQQIDHAKQ LIEEKIEGPL
510 520 530 540 550
CPVGPGPGGP GPAGPMGPFN PGPFNQGPPG APPHAGGPPP HQYPPQGWGN
560 570 580 590 600
TYPQWQPPAP HDPNKAAAAA TDPNAAWAAY YSHYYQQPPG PVPGPAPAPA
610 620 630 640 650
APPAQGEPPQ PPPTGQSDYT KAWEEYYKKI GQQPQQPGAP PQQDYTKAWE
660 670 680 690 700
EYYKKQAQVA TGGGPGAPPG SQPDYSAAWA EYYRQQAAYY GQTPGPGGPQ
710 720 730 740
PPPTQQGQQQ ASGNCHPPPP PFSFQPPATV HPALVGSAGN PFPCGVCP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3B2WCD8 | A0A3B2WCD8_MOUSE | Far upstream element-binding protei... | Khsrp | 721 | Annotation score: | ||
A0A3B2W465 | A0A3B2W465_MOUSE | Far upstream element-binding protei... | Khsrp | 238 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 387 | I → F in BAE33750 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK156541 mRNA Translation: BAE33750.1 CT571247 Genomic DNA No translation available. BC064454 mRNA Translation: AAH64454.1 BC108414 mRNA Translation: AAI08415.1 |
CCDSi | CCDS50157.1 |
RefSeqi | NP_034743.3, NM_010613.3 |
Genome annotation databases
Ensembli | ENSMUST00000007814; ENSMUSP00000007814; ENSMUSG00000007670 |
GeneIDi | 16549 |
KEGGi | mmu:16549 |
UCSCi | uc008ddr.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK156541 mRNA Translation: BAE33750.1 CT571247 Genomic DNA No translation available. BC064454 mRNA Translation: AAH64454.1 BC108414 mRNA Translation: AAI08415.1 |
CCDSi | CCDS50157.1 |
RefSeqi | NP_034743.3, NM_010613.3 |
3D structure databases
SMRi | Q3U0V1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200927, 21 interactors |
IntActi | Q3U0V1, 7 interactors |
STRINGi | 10090.ENSMUSP00000007814 |
PTM databases
iPTMneti | Q3U0V1 |
PhosphoSitePlusi | Q3U0V1 |
SwissPalmi | Q3U0V1 |
Proteomic databases
EPDi | Q3U0V1 |
jPOSTi | Q3U0V1 |
MaxQBi | Q3U0V1 |
PaxDbi | Q3U0V1 |
PeptideAtlasi | Q3U0V1 |
PRIDEi | Q3U0V1 |
Protocols and materials databases
Antibodypediai | 6316, 299 antibodies |
DNASUi | 16549 |
Genome annotation databases
Ensembli | ENSMUST00000007814; ENSMUSP00000007814; ENSMUSG00000007670 |
GeneIDi | 16549 |
KEGGi | mmu:16549 |
UCSCi | uc008ddr.2, mouse |
Organism-specific databases
CTDi | 8570 |
MGIi | MGI:1336214, Khsrp |
Phylogenomic databases
eggNOGi | KOG1676, Eukaryota |
GeneTreei | ENSGT00940000156051 |
HOGENOMi | CLU_014285_1_0_1 |
InParanoidi | Q3U0V1 |
OMAi | HIMGPPE |
OrthoDBi | 590738at2759 |
TreeFami | TF313654 |
Enzyme and pathway databases
Reactomei | R-MMU-450604, KSRP (KHSRP) binds and destabilizes mRNA |
Miscellaneous databases
BioGRID-ORCSi | 16549, 0 hits in 17 CRISPR screens |
ChiTaRSi | Khsrp, mouse |
PROi | PR:Q3U0V1 |
RNActi | Q3U0V1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000007670, Expressed in intestine and 254 other tissues |
ExpressionAtlasi | Q3U0V1, baseline and differential |
Genevisiblei | Q3U0V1, MM |
Family and domain databases
Gene3Di | 3.30.1370.10, 4 hits |
InterProi | View protein in InterPro IPR015096, FUBP_C IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf |
Pfami | View protein in Pfam PF09005, DUF1897, 2 hits PF00013, KH_1, 4 hits |
SMARTi | View protein in SMART SM00322, KH, 4 hits |
SUPFAMi | SSF54791, SSF54791, 4 hits |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit PS50084, KH_TYPE_1, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FUBP2_MOUSE | |
Accessioni | Q3U0V1Primary (citable) accession number: Q3U0V1 Secondary accession number(s): E9QKH3, Q2VPQ6, Q6P2L2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 21, 2007 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families