UniProtKB - Q3U0P1 (PALB2_MOUSE)
Partner and localizer of BRCA2
Palb2
Functioni
Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity (By similarity).
By similarityGO - Molecular functioni
- DNA binding Source: UniProtKB
GO - Biological processi
- animal organ morphogenesis Source: MGI
- double-strand break repair via homologous recombination Source: UniProtKB
- embryonic organ development Source: MGI
- inner cell mass cell proliferation Source: MGI
- in utero embryonic development Source: MGI
- mesoderm development Source: MGI
- multicellular organism growth Source: MGI
- negative regulation of apoptotic process Source: MGI
- post-anal tail morphogenesis Source: MGI
- somitogenesis Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA recombination, DNA repair |
Enzyme and pathway databases
Reactomei | R-MMU-5685942, HDR through Homologous Recombination (HRR) R-MMU-5693568, Resolution of D-loop Structures through Holliday Junction Intermediates R-MMU-5693579, Homologous DNA Pairing and Strand Exchange |
Names & Taxonomyi
Protein namesi | Recommended name: Partner and localizer of BRCA2 |
Gene namesi | Name:Palb2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3040695, Palb2 |
VEuPathDBi | HostDB:ENSMUSG00000044702 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Colocalizes with BRCA2 in nuclear foci.By similarity
Nucleus
- nucleoplasm Source: MGI
Other locations
- DNA repair complex Source: Ensembl
- protein-containing complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000252392 | 1 – 1104 | Partner and localizer of BRCA2Add BLAST | 1104 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 274 | PhosphoserineBy similarity | 1 | |
Modified residuei | 364 | PhosphoserineBy similarity | 1 | |
Modified residuei | 432 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q3U0P1 |
MaxQBi | Q3U0P1 |
PaxDbi | Q3U0P1 |
PRIDEi | Q3U0P1 |
ProteomicsDBi | 295454 [Q3U0P1-1] 295455 [Q3U0P1-2] 295456 [Q3U0P1-3] 295457 [Q3U0P1-4] |
PTM databases
iPTMneti | Q3U0P1 |
PhosphoSitePlusi | Q3U0P1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000044702, Expressed in cardiac ventricle and 176 other tissues |
Genevisiblei | Q3U0P1, MM |
Interactioni
Subunit structurei
Homooligomer; dissociated upon DNA damage thus allowing association with BRCA1. Oligomerization is essential for its focal accumulation at DNA breaks.
Part of a BRCA complex containing BRCA1, BRCA2 and PALB2.
Interacts with BRCA1 and this interaction is essential for its function in HRR.
Interacts with RAD51AP1 and MORF4L1/MRG15.
Component of the homologous recombination repair (HR) complex composed of ERCC5/XPG, BRCA2, PALB2, DSS1 and RAD51 (By similarity). Within the complex, interacts with ERCC5/XPG and BRCA2 (By similarity).
Interacts with BRCA2, RAD51C, RAD51 and XRCC3; the interactions are direct and it may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3.
Interacts with POLH; the interaction is direct (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 231454, 9 interactors |
IntActi | Q3U0P1, 1 interactor |
MINTi | Q3U0P1 |
STRINGi | 10090.ENSMUSP00000095675 |
Miscellaneous databases
RNActi | Q3U0P1, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q3U0P1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 772 – 833 | WD 1Add BLAST | 62 | |
Repeati | 835 – 879 | WD 2Add BLAST | 45 | |
Repeati | 880 – 927 | WD 3Add BLAST | 48 | |
Repeati | 928 – 970 | WD 4Add BLAST | 43 | |
Repeati | 976 – 1027 | WD 5Add BLAST | 52 | |
Repeati | 1033 – 1071 | WD 6Add BLAST | 39 | |
Repeati | 1073 – 1104 | WD 7Add BLAST | 32 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 308 | Interaction with BRCA1By similarityAdd BLAST | 308 | |
Regioni | 1 – 195 | Interaction with RAD51By similarityAdd BLAST | 195 | |
Regioni | 1 – 157 | Required for its oligomerization and is important for its focal concentration at DNA damage sitesBy similarityAdd BLAST | 157 | |
Regioni | 39 – 91 | DisorderedSequence analysisAdd BLAST | 53 | |
Regioni | 122 – 157 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 243 – 272 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 304 – 354 | DisorderedSequence analysisAdd BLAST | 51 | |
Regioni | 374 – 424 | ChAM (Chromatin-association motif); required for chromatin association, mediates nucleosome associationBy similarityAdd BLAST | 51 | |
Regioni | 417 – 494 | DisorderedSequence analysisAdd BLAST | 78 | |
Regioni | 581 – 730 | DisorderedSequence analysisAdd BLAST | 150 | |
Regioni | 693 – 1104 | Required for interaction with POLH and POLH DNA synthesis stimulationBy similarityAdd BLAST | 412 | |
Regioni | 771 – 1104 | Interaction with RAD51 and BRCA2By similarityAdd BLAST | 334 | |
Regioni | 771 – 1104 | Interaction with RAD51, BRCA2 and POLHBy similarityAdd BLAST | 334 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 9 – 48 | Sequence analysisAdd BLAST | 40 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 58 – 91 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 122 – 150 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 305 – 341 | Polar residuesSequence analysisAdd BLAST | 37 | |
Compositional biasi | 429 – 464 | Polar residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 612 – 626 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 659 – 730 | Polar residuesSequence analysisAdd BLAST | 72 |
Domaini
Keywords - Domaini
Coiled coil, Repeat, WD repeatPhylogenomic databases
eggNOGi | ENOG502QRAP, Eukaryota |
GeneTreei | ENSGT00390000014423 |
HOGENOMi | CLU_008217_1_0_1 |
InParanoidi | Q3U0P1 |
OMAi | GHCQKED |
OrthoDBi | 710645at2759 |
PhylomeDBi | Q3U0P1 |
TreeFami | TF351544 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR042417, PALB2 IPR031920, PALB2_WD40 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR14662, PTHR14662, 2 hits |
Pfami | View protein in Pfam PF16756, PALB2_WD40, 1 hit |
SUPFAMi | SSF50978, SSF50978, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEELSGKPLS YAEKEKLKEK LAFLKKEYSR TLARLQRAKR AEKAKNSKKA
60 70 80 90 100
IEDGVPQPEA SSQLSHSESI NKGFPCDTLQ SNHLDEETGE NISQILDVEP
110 120 130 140 150
QSFNCKQGKE VLHTPRAGDI QGQLLHSTSS PDGKKEQNTL PGTTKTPWEK
160 170 180 190 200
SSVSQEKEDY FDTNSLALLG KHRKGQESIS RKNSRTPVSE KTHLLSLRSQ
210 220 230 240 250
IPDPPALVTG IGEGILIPPS GKSERGIDTL VRGNTVSAEA AVPSCTASNS
260 270 280 290 300
NHSQHLEHTP PKSGCKITTQ GPASSTNLVA QDQKMTIFTV NSVVYKAVRA
310 320 330 340 350
HGQLPGSPNS CSVNDLTHSN LPANSTPNSK SLKSPSNTVD ERNEPLQEDE
360 370 380 390 400
ILGPSKNFNL AAVSPPSTES QIHSCTMLEG LLFPAEYYVR TTRRMSDCQR
410 420 430 440 450
KIALEAVIQS HLGVKKKELK KKTKATKAVV LSSEDTDQSE SGMLDTSTGQ
460 470 480 490 500
SSSGSLSQKL LSPAEVSSPP GPAGKATTPP PGRGHRGKRK SARTSTLGHC
510 520 530 540 550
QLLFPPCAAL AVNRSKGKFT KHKCQNRGVV IHDFELPDED FGLLKLEKLK
560 570 580 590 600
SCSEKLIESP DSKNCGERLP REGNHAALEE LQRDSETEGL EEELTVPPGE
610 620 630 640 650
AYRPGPTLRR QPGSKDLSSS IVLFTPADTA APNDSGRPPP SLCSPAFPIL
660 670 680 690 700
GMTPALGSQA AGETLSTEAA QPCSTSQPPL LGDTNSLVNN SKQCNSSACS
710 720 730 740 750
PKPDTNLQAS GRQGQPACDS DSGPQATPLP VESFTFRENQ LCGNACLELH
760 770 780 790 800
EHSTEQTETA DRPACDNLNP GNLQLVSELK NPSSSCSVDV SAMWWERAGA
810 820 830 840 850
KEPCIVTACE DVVSLWKPLN SLQWEKVHTW HFTEVPVLQI VPVPDVYNLI
860 870 880 890 900
CVALGSLEIR EIRALLCSSG DDSEKQVLLK SGDIKAMLGL TKRRLVSSTG
910 920 930 940 950
TFCNQQIQIM TFADDGSSKD EQLLMPPDET VLTFAEVQGT QEALLGTTTV
960 970 980 990 1000
NSIVIWNLKT GQLLKKMHID DSYQASVCHG AYSEKGLLFV VVSQPCAKES
1010 1020 1030 1040 1050
QALGSPVFQL LVINPKTAQS VGVLLCSLPQ GQAGRFLEGD VKDHVAAAVL
1060 1070 1080 1090 1100
TSGTIAIWDL LLGHCTALLP PVSDQSWSLV KWSGTDSHLL AGQKDGNIFI
YRYF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YVU6 | D3YVU6_MOUSE | Partner and localizer of BRCA2 | Palb2 | 1,030 | Annotation score: | ||
D6RDD1 | D6RDD1_MOUSE | Partner and localizer of BRCA2 | Palb2 | 38 | Annotation score: | ||
F6VTV6 | F6VTV6_MOUSE | Partner and localizer of BRCA2 | Palb2 | 18 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020928 | 36 – 755 | Missing in isoform 3. 1 PublicationAdd BLAST | 720 | |
Alternative sequenceiVSP_020929 | 170 – 532 | Missing in isoform 2. 1 PublicationAdd BLAST | 363 | |
Alternative sequenceiVSP_020930 | 533 – 544 | DFELP…DFGLL → GKSRRRVRLRLM in isoform 4. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_020931 | 545 – 1104 | Missing in isoform 4. 1 PublicationAdd BLAST | 560 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028653 mRNA Translation: BAC26048.1 AK156701 mRNA Translation: BAE33811.1 BC055302 mRNA Translation: AAH55302.1 BC066140 mRNA Translation: AAH66140.1 |
CCDSi | CCDS40117.1 [Q3U0P1-1] CCDS72035.1 [Q3U0P1-2] CCDS80796.1 [Q3U0P1-3] |
RefSeqi | NP_001074707.1, NM_001081238.2 [Q3U0P1-1] NP_001276771.1, NM_001289842.1 NP_001276772.1, NM_001289843.1 NP_001276773.1, NM_001289844.1 [Q3U0P1-2] NP_001276774.1, NM_001289845.1 [Q3U0P1-3] |
Genome annotation databases
Ensembli | ENSMUST00000063587; ENSMUSP00000063514; ENSMUSG00000044702 [Q3U0P1-3] ENSMUST00000098068; ENSMUSP00000095675; ENSMUSG00000044702 [Q3U0P1-1] ENSMUST00000106469; ENSMUSP00000102077; ENSMUSG00000044702 [Q3U0P1-2] |
GeneIDi | 233826 |
KEGGi | mmu:233826 |
UCSCi | uc009joj.2, mouse [Q3U0P1-1] uc009jom.1, mouse [Q3U0P1-4] uc012ftg.2, mouse [Q3U0P1-2] uc012fth.2, mouse [Q3U0P1-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028653 mRNA Translation: BAC26048.1 AK156701 mRNA Translation: BAE33811.1 BC055302 mRNA Translation: AAH55302.1 BC066140 mRNA Translation: AAH66140.1 |
CCDSi | CCDS40117.1 [Q3U0P1-1] CCDS72035.1 [Q3U0P1-2] CCDS80796.1 [Q3U0P1-3] |
RefSeqi | NP_001074707.1, NM_001081238.2 [Q3U0P1-1] NP_001276771.1, NM_001289842.1 NP_001276772.1, NM_001289843.1 NP_001276773.1, NM_001289844.1 [Q3U0P1-2] NP_001276774.1, NM_001289845.1 [Q3U0P1-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6E4H | NMR | - | A/B | 1-60 | [»] | |
7K3S | NMR | - | B | 1-60 | [»] | |
SMRi | Q3U0P1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 231454, 9 interactors |
IntActi | Q3U0P1, 1 interactor |
MINTi | Q3U0P1 |
STRINGi | 10090.ENSMUSP00000095675 |
PTM databases
iPTMneti | Q3U0P1 |
PhosphoSitePlusi | Q3U0P1 |
Proteomic databases
EPDi | Q3U0P1 |
MaxQBi | Q3U0P1 |
PaxDbi | Q3U0P1 |
PRIDEi | Q3U0P1 |
ProteomicsDBi | 295454 [Q3U0P1-1] 295455 [Q3U0P1-2] 295456 [Q3U0P1-3] 295457 [Q3U0P1-4] |
Protocols and materials databases
Antibodypediai | 26008, 239 antibodies from 35 providers |
DNASUi | 233826 |
Genome annotation databases
Ensembli | ENSMUST00000063587; ENSMUSP00000063514; ENSMUSG00000044702 [Q3U0P1-3] ENSMUST00000098068; ENSMUSP00000095675; ENSMUSG00000044702 [Q3U0P1-1] ENSMUST00000106469; ENSMUSP00000102077; ENSMUSG00000044702 [Q3U0P1-2] |
GeneIDi | 233826 |
KEGGi | mmu:233826 |
UCSCi | uc009joj.2, mouse [Q3U0P1-1] uc009jom.1, mouse [Q3U0P1-4] uc012ftg.2, mouse [Q3U0P1-2] uc012fth.2, mouse [Q3U0P1-3] |
Organism-specific databases
CTDi | 79728 |
MGIi | MGI:3040695, Palb2 |
VEuPathDBi | HostDB:ENSMUSG00000044702 |
Phylogenomic databases
eggNOGi | ENOG502QRAP, Eukaryota |
GeneTreei | ENSGT00390000014423 |
HOGENOMi | CLU_008217_1_0_1 |
InParanoidi | Q3U0P1 |
OMAi | GHCQKED |
OrthoDBi | 710645at2759 |
PhylomeDBi | Q3U0P1 |
TreeFami | TF351544 |
Enzyme and pathway databases
Reactomei | R-MMU-5685942, HDR through Homologous Recombination (HRR) R-MMU-5693568, Resolution of D-loop Structures through Holliday Junction Intermediates R-MMU-5693579, Homologous DNA Pairing and Strand Exchange |
Miscellaneous databases
BioGRID-ORCSi | 233826, 24 hits in 102 CRISPR screens |
ChiTaRSi | Palb2, mouse |
PROi | PR:Q3U0P1 |
RNActi | Q3U0P1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000044702, Expressed in cardiac ventricle and 176 other tissues |
Genevisiblei | Q3U0P1, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR042417, PALB2 IPR031920, PALB2_WD40 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR14662, PTHR14662, 2 hits |
Pfami | View protein in Pfam PF16756, PALB2_WD40, 1 hit |
SUPFAMi | SSF50978, SSF50978, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PALB2_MOUSE | |
Accessioni | Q3U0P1Primary (citable) accession number: Q3U0P1 Secondary accession number(s): Q6NZG9, Q7TMQ4, Q8CEA9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | October 17, 2006 | |
Last modified: | February 23, 2022 | |
This is version 129 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references