UniProtKB - Q3U0D9 (HACE1_MOUSE)
E3 ubiquitin-protein ligase HACE1
Hace1
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.26
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 876 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- Rab GTPase binding Source: UniProtKB
- Rac GTPase binding Source: MGI
- ubiquitin protein ligase activity Source: GO_Central
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- Golgi organization Source: UniProtKB
- membrane fusion Source: UniProtKB
- positive regulation of protein catabolic process Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein K48-linked ubiquitination Source: UniProtKB
- protein polyubiquitination Source: GO_Central
- protein ubiquitination Source: UniProtKB
- regulation of cell migration Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Cell cycle, Transcription, Transcription regulation, Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase HACE1 (EC:2.3.2.26)Alternative name(s): HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 HECT-type E3 ubiquitin transferase HACE1 |
Gene namesi | Name:Hace1 Synonyms:Kiaa1320 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2446110, Hace1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Golgi apparatus
- Golgi stack membrane By similarity
Other locations
- Cytoplasm By similarity
Note: A significant portion localizes to the endoplasmic reticulum. Targeted to Golgi membrane via its interaction with Rab proteins (By similarity).By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Golgi apparatus
- Golgi cisterna membrane Source: UniProtKB-SubCell
- Golgi membrane Source: UniProtKB
Nucleus
- nuclear body Source: MGI
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000280623 | 1 – 909 | E3 ubiquitin-protein ligase HACE1Add BLAST | 909 |
Proteomic databases
MaxQBi | Q3U0D9 |
PaxDbi | Q3U0D9 |
PeptideAtlasi | Q3U0D9 |
PRIDEi | Q3U0D9 |
PTM databases
iPTMneti | Q3U0D9 |
PhosphoSitePlusi | Q3U0D9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000038822, Expressed in liver and 280 other tissues |
Genevisiblei | Q3U0D9, MM |
Interactioni
Subunit structurei
Interacts with RAB1 (RAB1A, RAB1B or RAB1C), RAB4 (RAB4A or RAB4B) and RAB11 (RAB11A or RAB11B); in a GTP-dependent manner.
Interacts with RAC1; in a GTP-dependent manner.
Interacts with the 26S proteasomal complex through the 20S core proteasomal subunit (By similarity).
Interacts with RARB.
By similarity1 PublicationGO - Molecular functioni
- Rab GTPase binding Source: UniProtKB
- Rac GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 229081, 4 interactors |
IntActi | Q3U0D9, 1 interactor |
STRINGi | 10090.ENSMUSP00000039206 |
Miscellaneous databases
RNActi | Q3U0D9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 64 – 93 | ANK 1Add BLAST | 30 | |
Repeati | 97 – 126 | ANK 2Add BLAST | 30 | |
Repeati | 130 – 159 | ANK 3Add BLAST | 30 | |
Repeati | 163 – 192 | ANK 4Add BLAST | 30 | |
Repeati | 196 – 226 | ANK 5Add BLAST | 31 | |
Repeati | 228 – 257 | ANK 6Add BLAST | 30 | |
Domaini | 574 – 909 | HECTPROSITE-ProRule annotationAdd BLAST | 336 |
Keywords - Domaini
ANK repeat, RepeatPhylogenomic databases
eggNOGi | KOG0939, Eukaryota KOG4177, Eukaryota |
GeneTreei | ENSGT00940000155839 |
HOGENOMi | CLU_015878_0_0_1 |
InParanoidi | Q3U0D9 |
OrthoDBi | 799706at2759 |
PhylomeDBi | Q3U0D9 |
TreeFami | TF323417 |
Family and domain databases
CDDi | cd00078, HECTc, 1 hit |
Gene3Di | 1.25.40.20, 2 hits |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF00632, HECT, 1 hit |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 6 hits SM00119, HECTc, 1 hit |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 5 hits PS50237, HECT, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MERAMEQLNR LTRSLRRART VELPEDNETA VYTLMPMVMA DQHRSVSELL
60 70 80 90 100
SNSKFDVNYA FGRVKRSLLH IAANCGSVEC LVLLLKKGAN PNYQDISGCT
110 120 130 140 150
PLHLAARNGQ KKCMSKLLEY SADVNICNNE GLTAIHWLAV NGRTELLHDL
160 170 180 190 200
VQHVTDVDVE DAMGQTALHV ACQNGHKTTV QCLLDSGADI NRPNVSGATP
210 220 230 240 250
LYFACSHGQR DTAQILLLRG AKYLPDKNGV TPLDLCVQGG YGQTCEVLIQ
260 270 280 290 300
YHPRLFQTIV QMTQNEDLRE NMLRQVLQHL SQQSESQYLK ILTGLAEVAT
310 320 330 340 350
TNGHKLLSLS SNYDAQMKSL LRIVRIFCHV FRIGPSSPSN GIDMGYNGNK
360 370 380 390 400
TPRSQVFKPL ELLWHSLDEW LVLIATELMK NKEDSTDITS ILLKQKGQDQ
410 420 430 440 450
EAPSLSAFEP PGPGSYESLP PGPGDSKPEV LAGEQEASAD CQDVISVTAN
460 470 480 490 500
RLSAVIQAFY MCCSCQMPPG MTSPRFIEFV CKHDEVLKCF VNRNPKIIFD
510 520 530 540 550
HFHFLLECPE LMSRFMHIIK AQPFKDRCEW FYEHLHSGQP DSDMVHRPVS
560 570 580 590 600
ENDILLVHRD SIFRSSCEIV SKANCAKLKQ GIAVRFHGEE GMGQGVVREW
610 620 630 640 650
FDILSNEIVN PDYALFTQSA DGTTFQPNSN SYVNPDHLNY FRFAGQILGL
660 670 680 690 700
ALNHRQLVNI YFTRSFYKHI LGIPVNYQDV ASIDPEYAKN LQWILDNDIS
710 720 730 740 750
DLGLELTFSV ETDVFGAMEE VPLKPGGGSI LVTQNNKAEY VQLVTELRMT
760 770 780 790 800
RAIQPQINAF LQGFHMFIPP SLIQLFDEYE LELLLSGMPE IDVNDWIKNT
810 820 830 840 850
EYTSGYERED PVIQWFWEVV EDITQEERVL LLQFVTGSSR VPHGGFANIM
860 870 880 890 900
GGSGLQNFTI AAVPYTPNLL PTSSTCINML KLPEYPSKEI LKDRLLVALH
CGSYGYTMA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH9KV10 | H9KV10_MOUSE | E3 ubiquitin-protein ligase HACE1 | Hace1 | 300 | Annotation score: | ||
H9KV09 | H9KV09_MOUSE | E3 ubiquitin-protein ligase HACE1 | Hace1 | 329 | Annotation score: | ||
F6T851 | F6T851_MOUSE | E3 ubiquitin-protein ligase HACE1 | Hace1 | 119 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 92 | N → S in BAC31390 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023832 | 738 – 781 | Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 44 | |
Alternative sequenceiVSP_042379 | 814 – 832 | Missing in isoform 4. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_023833 | 838 | S → R in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_023834 | 839 – 909 | Missing in isoform 2. 1 PublicationAdd BLAST | 71 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK042879 mRNA Translation: BAC31390.1 AK156958 mRNA Translation: BAE33915.1 AC135669 Genomic DNA No translation available. AC153847 Genomic DNA No translation available. BC025227 mRNA Translation: AAH25227.1 BC025474 mRNA Translation: AAH25474.1 Different initiation. BC120695 mRNA Translation: AAI20696.1 BC120697 mRNA Translation: AAI20698.1 AK220383 mRNA Translation: BAD90440.1 |
CCDSi | CCDS23829.1 [Q3U0D9-1] |
RefSeqi | NP_766061.2, NM_172473.3 [Q3U0D9-1] |
Genome annotation databases
Ensembli | ENSMUST00000037044; ENSMUSP00000039206; ENSMUSG00000038822 [Q3U0D9-1] |
GeneIDi | 209462 |
KEGGi | mmu:209462 |
UCSCi | uc007fad.2, mouse [Q3U0D9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK042879 mRNA Translation: BAC31390.1 AK156958 mRNA Translation: BAE33915.1 AC135669 Genomic DNA No translation available. AC153847 Genomic DNA No translation available. BC025227 mRNA Translation: AAH25227.1 BC025474 mRNA Translation: AAH25474.1 Different initiation. BC120695 mRNA Translation: AAI20696.1 BC120697 mRNA Translation: AAI20698.1 AK220383 mRNA Translation: BAD90440.1 |
CCDSi | CCDS23829.1 [Q3U0D9-1] |
RefSeqi | NP_766061.2, NM_172473.3 [Q3U0D9-1] |
3D structure databases
SMRi | Q3U0D9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229081, 4 interactors |
IntActi | Q3U0D9, 1 interactor |
STRINGi | 10090.ENSMUSP00000039206 |
PTM databases
iPTMneti | Q3U0D9 |
PhosphoSitePlusi | Q3U0D9 |
Proteomic databases
MaxQBi | Q3U0D9 |
PaxDbi | Q3U0D9 |
PeptideAtlasi | Q3U0D9 |
PRIDEi | Q3U0D9 |
Protocols and materials databases
Antibodypediai | 32098, 203 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000037044; ENSMUSP00000039206; ENSMUSG00000038822 [Q3U0D9-1] |
GeneIDi | 209462 |
KEGGi | mmu:209462 |
UCSCi | uc007fad.2, mouse [Q3U0D9-1] |
Organism-specific databases
CTDi | 57531 |
MGIi | MGI:2446110, Hace1 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0939, Eukaryota KOG4177, Eukaryota |
GeneTreei | ENSGT00940000155839 |
HOGENOMi | CLU_015878_0_0_1 |
InParanoidi | Q3U0D9 |
OrthoDBi | 799706at2759 |
PhylomeDBi | Q3U0D9 |
TreeFami | TF323417 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 209462, 4 hits in 17 CRISPR screens |
ChiTaRSi | Hace1, mouse |
PROi | PR:Q3U0D9 |
RNActi | Q3U0D9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038822, Expressed in liver and 280 other tissues |
Genevisiblei | Q3U0D9, MM |
Family and domain databases
CDDi | cd00078, HECTc, 1 hit |
Gene3Di | 1.25.40.20, 2 hits |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF00632, HECT, 1 hit |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 6 hits SM00119, HECTc, 1 hit |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 5 hits PS50237, HECT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HACE1_MOUSE | |
Accessioni | Q3U0D9Primary (citable) accession number: Q3U0D9 Secondary accession number(s): F6VQI5 Q8R3G4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | October 11, 2005 | |
Last modified: | December 2, 2020 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways