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Protein

Extended synaptotagmin-2

Gene

Esyt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tethers the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane. Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport. Plays a role in FGF signaling via its role in the rapid internalization of FGFR1 that has been activated by FGF1 binding; this occurs most likely via the AP-2 complex (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi324Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi325Calcium 1By similarity1
Metal bindingi325Calcium 2By similarity1
Metal bindingi337Calcium 2By similarity1
Metal bindingi384Calcium 1By similarity1
Metal bindingi384Calcium 2By similarity1
Metal bindingi385Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi386Calcium 1By similarity1
Metal bindingi386Calcium 2By similarity1
Metal bindingi386Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi388Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi390Calcium 3By similarity1
Metal bindingi391Calcium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis, Lipid transport, Transport
LigandCalcium, Lipid-binding, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660662 Glycosphingolipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Extended synaptotagmin-2
Short name:
E-Syt2
Gene namesi
Name:Esyt2
Synonyms:D12Ertd551e, Fam62b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1261845 Esyt2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 27CytoplasmicSequence analysisAdd BLAST27
Transmembranei28 – 48HelicalSequence analysisAdd BLAST21
Topological domaini49 – 51LumenalSequence analysis3
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Topological domaini73 – 845CytoplasmicSequence analysisAdd BLAST773

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782591 – 845Extended synaptotagmin-2Add BLAST845

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei615PhosphoserineBy similarity1
Modified residuei617PhosphoserineBy similarity1
Modified residuei629PhosphothreonineBy similarity1
Modified residuei660PhosphoserineCombined sources1
Modified residuei662PhosphoserineCombined sources1
Modified residuei663PhosphoserineCombined sources1
Modified residuei667PhosphoserineCombined sources1
Modified residuei679PhosphoserineCombined sources1
Modified residuei682PhosphoserineCombined sources1
Modified residuei685PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3TZZ7
MaxQBiQ3TZZ7
PaxDbiQ3TZZ7
PeptideAtlasiQ3TZZ7
PRIDEiQ3TZZ7

PTM databases

iPTMnetiQ3TZZ7
PhosphoSitePlusiQ3TZZ7

Expressioni

Gene expression databases

BgeeiENSMUSG00000021171 Expressed in 240 organ(s), highest expression level in dorsal pancreas
CleanExiMM_D12ERTD551E
ExpressionAtlasiQ3TZZ7 baseline and differential
GenevisibleiQ3TZZ7 MM

Interactioni

Subunit structurei

Homodimer. Interacts with ESYT1 and ESYT3. Interacts with FGFR1 that has been activated by FGF1 binding. Interacts with the AP-2 complex; identified in a complex with the AP-2 complex and FGFR1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206705, 1 interactor
IntActiQ3TZZ7, 1 interactor
MINTiQ3TZZ7
STRINGi10090.ENSMUSP00000098548

Structurei

3D structure databases

ProteinModelPortaliQ3TZZ7
SMRiQ3TZZ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini115 – 294SMP-LTDPROSITE-ProRule annotationAdd BLAST180
Domaini296 – 397C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini447 – 541C2 2PROSITE-ProRule annotationAdd BLAST95
Domaini712 – 816C2 3PROSITE-ProRule annotationAdd BLAST105

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni757 – 764Required for phosphatidylinositol 4,5-bisphosphate-dependent location at the cell membraneBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi660 – 663Poly-Ser4

Domaini

Anchored to the endoplasmic reticulum membrane by a transmembrane hairpin structure; both N-terminus and C-terminus are cytoplasmic.By similarity
The C2 domains mediate lipid and calcium binding. The N-terminal C2 domain binds calcium ions and is important for calcium-dependent lipid binding and interaction with membranes. Two calcium ions are bound at a high-affinity site and a third calcium ion is bound with lower affinity. May bind up to four calcium ions. In contrast, the second C2 domain apparently does not bind calcium. The third C2 domain mediates interaction with membranes enriched in phosphatidylinositol 4,5-bisphosphate and is required for location at the cell membrane (By similarity).By similarity
The SMP-LTD domain is a barrel-like domain that binds glycerophospholipids in its interior; can bind two lipid molecules simultaneously. Binds a variety of lipids, including phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol (By similarity).By similarity

Sequence similaritiesi

Belongs to the extended synaptotagmin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ISCC Eukaryota
ENOG410XPR4 LUCA
GeneTreeiENSGT00550000074417
HOGENOMiHOG000043080
HOVERGENiHBG055795
InParanoidiQ3TZZ7
OMAiGVIKDNC
OrthoDBiEOG091G02X7
PhylomeDBiQ3TZZ7
TreeFamiTF324255

Family and domain databases

CDDicd08391 C2A_C2C_Synaptotagmin_like, 1 hit
cd04050 C2B_Synaptotagmin-like, 1 hit
cd04030 C2C_KIAA1228, 1 hit
Gene3Di2.60.40.150, 3 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR037752 C2C_KIAA1228
IPR037733 Ext_Synaptotagmin_C2A
IPR037749 Ext_Synaptotagmin_C2B
IPR031468 SMP_LBD
IPR039010 Synaptotagmin_SMP
PfamiView protein in Pfam
PF00168 C2, 3 hits
PF17047 SMP_LBD, 1 hit
SMARTiView protein in SMART
SM00239 C2, 3 hits
PROSITEiView protein in PROSITE
PS50004 C2, 3 hits
PS51847 SMP, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q3TZZ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSAGGEGPE AGPGRAGGRS EPEAPGSALS VDLPGLLGQL ARSFALLLPV
60 70 80 90 100
YALGYLGLSF SWVLLALGLL AWCRRSRGLK ASRLCRALAL LEDEEQAVRL
110 120 130 140 150
GVRACDLPAW VHFPDTERAE WLNKTVKHMW PFICQFIEKL FRETIEPAVR
160 170 180 190 200
GANAHLSTFS FTKVDVGQQP LRVNGVKVYT ENVDKRQIIL DLQISFVGNC
210 220 230 240 250
EIDLEIKRYF CRAGVKSIQI HGTMRVILEP LIGDMPLVGA LSIFFLRKPL
260 270 280 290 300
LEINWTGLTN LLDIPGLNGL SDTIILDIIS NYLVLPNRIT VPLVSEVQIA
310 320 330 340 350
QLRFPIPKGV LRIHFIEAQD LQGKDTYLKG LVKGKSDPYG IIRVGNQIFQ
360 370 380 390 400
SKVIKENLSP KWNEVYEALV YEHPGQELEI ELFDEDPDKD DFLGSLMIDL
410 420 430 440 450
IEVEKERLLD EWFTLDEVPK GKLHLKLEWL TLMPDAANLD KVLADIRADK
460 470 480 490 500
DQASDGLSSA LLILYLDSAR NLPSGKKINS NPNPLVQMSV GHKAQESKIR
510 520 530 540 550
YKTSEPVWEE NFTFFIHNPR RQDLEVEVKD EQHQCSLGSL RIPLSQLLTS
560 570 580 590 600
DNMTINQRFQ LSNSGPNSTL KMKIALRVLH LEKQERPPDY QHSAQVKRPS
610 620 630 640 650
VSKEGRKMPI KSQMSASPGT GGANTAPSTP VMGVDDKPAM EEKPQPPEAS
660 670 680 690 700
PLGHRDLGRS SSSLLASPSH IAAKEPTPSI ASDISLPIAT QELRQRLRQL
710 720 730 740 750
ENGTTLGQSP LGQIQLTIRH SSQRNKLIVV VHSCRNLIAF SEDGSDPYVR
760 770 780 790 800
MYLLPDKRRS GRRKTHVSKK TLNPVFDQSF DFSVSLPEVQ RRTLDVAVKN
810 820 830 840
SGGFLSKDKG LLGKVLVVLA SEELAKGWTQ WYDLTEDGTR PQVIT
Length:845
Mass (Da):94,139
Last modified:October 11, 2005 - v1
Checksum:i988F3D97D7F3CF6C
GO
Isoform 2 (identifier: Q3TZZ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-487: Missing.

Show »
Length:358
Mass (Da):39,884
Checksum:i6AE8B94A52027E24
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1Y7VM39A0A1Y7VM39_MOUSE
Extended synaptotagmin-2
Esyt2
326Annotation score:
A0A1Y7VNA1A0A1Y7VNA1_MOUSE
Extended synaptotagmin-2
Esyt2
89Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0232431 – 487Missing in isoform 2. 2 PublicationsAdd BLAST487

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014813 mRNA Translation: BAB29565.1
AK044360 mRNA Translation: BAC31884.1
AK157352 mRNA Translation: BAE34059.1
BC052440 mRNA Translation: AAH52440.1
BC054797 mRNA Translation: AAH54797.1
BC059230 mRNA Translation: AAH59230.1
BC138937 mRNA Translation: AAI38938.1
CCDSiCCDS26211.1 [Q3TZZ7-1]
RefSeqiNP_083007.2, NM_028731.5 [Q3TZZ7-1]
XP_017170617.1, XM_017315128.1 [Q3TZZ7-1]
UniGeneiMm.273755

Genome annotation databases

EnsembliENSMUST00000100986; ENSMUSP00000098548; ENSMUSG00000021171 [Q3TZZ7-1]
ENSMUST00000220816; ENSMUSP00000152444; ENSMUSG00000021171 [Q3TZZ7-1]
GeneIDi52635
KEGGimmu:52635
UCSCiuc007pho.1 mouse [Q3TZZ7-1]
uc007phq.1 mouse [Q3TZZ7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014813 mRNA Translation: BAB29565.1
AK044360 mRNA Translation: BAC31884.1
AK157352 mRNA Translation: BAE34059.1
BC052440 mRNA Translation: AAH52440.1
BC054797 mRNA Translation: AAH54797.1
BC059230 mRNA Translation: AAH59230.1
BC138937 mRNA Translation: AAI38938.1
CCDSiCCDS26211.1 [Q3TZZ7-1]
RefSeqiNP_083007.2, NM_028731.5 [Q3TZZ7-1]
XP_017170617.1, XM_017315128.1 [Q3TZZ7-1]
UniGeneiMm.273755

3D structure databases

ProteinModelPortaliQ3TZZ7
SMRiQ3TZZ7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206705, 1 interactor
IntActiQ3TZZ7, 1 interactor
MINTiQ3TZZ7
STRINGi10090.ENSMUSP00000098548

PTM databases

iPTMnetiQ3TZZ7
PhosphoSitePlusiQ3TZZ7

Proteomic databases

EPDiQ3TZZ7
MaxQBiQ3TZZ7
PaxDbiQ3TZZ7
PeptideAtlasiQ3TZZ7
PRIDEiQ3TZZ7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100986; ENSMUSP00000098548; ENSMUSG00000021171 [Q3TZZ7-1]
ENSMUST00000220816; ENSMUSP00000152444; ENSMUSG00000021171 [Q3TZZ7-1]
GeneIDi52635
KEGGimmu:52635
UCSCiuc007pho.1 mouse [Q3TZZ7-1]
uc007phq.1 mouse [Q3TZZ7-2]

Organism-specific databases

CTDi57488
MGIiMGI:1261845 Esyt2

Phylogenomic databases

eggNOGiENOG410ISCC Eukaryota
ENOG410XPR4 LUCA
GeneTreeiENSGT00550000074417
HOGENOMiHOG000043080
HOVERGENiHBG055795
InParanoidiQ3TZZ7
OMAiGVIKDNC
OrthoDBiEOG091G02X7
PhylomeDBiQ3TZZ7
TreeFamiTF324255

Enzyme and pathway databases

ReactomeiR-MMU-1660662 Glycosphingolipid metabolism

Miscellaneous databases

PROiPR:Q3TZZ7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021171 Expressed in 240 organ(s), highest expression level in dorsal pancreas
CleanExiMM_D12ERTD551E
ExpressionAtlasiQ3TZZ7 baseline and differential
GenevisibleiQ3TZZ7 MM

Family and domain databases

CDDicd08391 C2A_C2C_Synaptotagmin_like, 1 hit
cd04050 C2B_Synaptotagmin-like, 1 hit
cd04030 C2C_KIAA1228, 1 hit
Gene3Di2.60.40.150, 3 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR037752 C2C_KIAA1228
IPR037733 Ext_Synaptotagmin_C2A
IPR037749 Ext_Synaptotagmin_C2B
IPR031468 SMP_LBD
IPR039010 Synaptotagmin_SMP
PfamiView protein in Pfam
PF00168 C2, 3 hits
PF17047 SMP_LBD, 1 hit
SMARTiView protein in SMART
SM00239 C2, 3 hits
PROSITEiView protein in PROSITE
PS50004 C2, 3 hits
PS51847 SMP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiESYT2_MOUSE
AccessioniPrimary (citable) accession number: Q3TZZ7
Secondary accession number(s): B2RSN5, Q9D5Y7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2005
Last modified: September 12, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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