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Protein

Polynucleotide 5'-hydroxyl-kinase NOL9

Gene

Nol9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi322 – 329ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, RNA-binding, Transferase
Biological processrRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Polynucleotide 5'-hydroxyl-kinase NOL9 (EC:2.7.1.-)
Alternative name(s):
Nucleolar protein 9Imported
Gene namesi
Name:Nol9Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1921285 Nol9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003674322 – 714Polynucleotide 5'-hydroxyl-kinase NOL9Add BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei128PhosphoserineCombined sources1
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei502PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ3TZX8
MaxQBiQ3TZX8
PaxDbiQ3TZX8
PeptideAtlasiQ3TZX8
PRIDEiQ3TZX8

PTM databases

iPTMnetiQ3TZX8
PhosphoSitePlusiQ3TZX8

Expressioni

Gene expression databases

BgeeiENSMUSG00000028948 Expressed in 227 organ(s), highest expression level in lumbar subsegment of spinal cord
ExpressionAtlasiQ3TZX8 baseline and differential
GenevisibleiQ3TZX8 MM

Interactioni

Subunit structurei

Interacts with PELP1, WDR18 and SENP3.1 Publication

Protein-protein interaction databases

BioGridi216440, 1 interactor
IntActiQ3TZX8, 3 interactors
MINTiQ3TZX8
STRINGi10090.ENSMUSP00000081133

Structurei

3D structure databases

ProteinModelPortaliQ3TZX8
SMRiQ3TZX8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 54Arg-richSequence analysisAdd BLAST46

Sequence similaritiesi

Belongs to the Clp1 family. NOL9/GRC3 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2750 Eukaryota
COG1341 LUCA
GeneTreeiENSGT00390000012406
HOVERGENiHBG058105
InParanoidiQ3TZX8
KOiK06947
OMAiMDGLYTK
OrthoDBiEOG091G0BNE
PhylomeDBiQ3TZX8
TreeFamiTF313802

Family and domain databases

InterProiView protein in InterPro
IPR032319 CLP1_P
PfamiView protein in Pfam
PF16575 CLP1_P, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 11 Publication (identifier: Q3TZX8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAESEVLHRR APSRSSWLRV RKARPHLLLS RRGRRRFGVL TRVELRRLRR
60 70 80 90 100
RLLRAHALGG DWKQVAPAGA HVAVKCKLRA RSRPAPRSPP TPSVPPAPCT
110 120 130 140 150
ASATCSLLNP RNHSTPQSRA GRPVRKVSPN VTQPVRDLGS GRVLMMLPPG
160 170 180 190 200
EGFTFSGICR VTCVYGQLEV YGHIINQGQP PQDVFSVYTH SYLTINGVPY
210 220 230 240 250
AEPEKSEKAI RREIRALLKP YTKLDDRNWV VRYFPPLGSI MILERMQSRF
260 270 280 290 300
VDFLKTYKCS SYVLLQENAP VRVNSEFTTL KKIGIRRQKR KKAICLSESG
310 320 330 340 350
LCALEELVSV SCDGCPVILL CGACDIGKST FNRILINQLL NSIPGVDYLE
360 370 380 390 400
CDLGQTEFTP PGCVALLTIT EPLLGPPYTH QRKPQRMVYY GKMNCYNDYE
410 420 430 440 450
NYIDIVKYVF RDYKREFPLI INTMGWVSDN GLRLLVDLIR VLSPNYVVQL
460 470 480 490 500
YSDRCKFTPT LTSEYVELTD GLYTKSKIKR YRGFEIPEFG DNLEFTYEEK
510 520 530 540 550
ESSPLPVFTG HVLLSVHSEF LSSKNEKNRA KYNRIFRDLA VLGYLSQLML
560 570 580 590 600
PVPESLSPLH SLTPYQVPFS AVAIRVLHAD VAPTHILYAV NASWVGLCRI
610 620 630 640 650
VDDMKGYTRG PILLAQNPIC DCLGFGICRG IDMDKRTYHI LTPLPPEELK
660 670 680 690 700
TVNCLLVGSI SIPHCIFQNQ PGPEGSVPYV TTDYNLNIPG ATEKIGEREY
710
GKAFPRHKLR QRRK
Note: No experimental confirmation available.Curated
Length:714
Mass (Da):80,840
Last modified:October 11, 2005 - v1
Checksum:iE2FFC860BD488F5C
GO
Isoform 22 Publications (identifier: Q3TZX8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     671-671: P → L
     672-714: Missing.

Show »
Length:671
Mass (Da):75,943
Checksum:i20CA7A273B8C3845
GO
Isoform 31 Publication (identifier: Q3TZX8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     627-627: I → E
     628-714: Missing.

Show »
Length:627
Mass (Da):70,969
Checksum:iB1417D4C14764BC1
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RPV7A0A0U1RPV7_MOUSE
Polynucleotide 5'-hydroxyl-kinase N...
Nol9
361Annotation score:

Sequence cautioni

The sequence AAH24877 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101A → G in BAC31300 (PubMed:16141072).Curated1
Sequence conflicti225D → E in BAB29433 (PubMed:16141072).Curated1
Sequence conflicti225D → E in CAM24579 (PubMed:19468303).Curated1
Sequence conflicti269A → S in BAC34472 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_053058627I → E in isoform 3. 1 Publication1
Alternative sequenceiVSP_053059628 – 714Missing in isoform 3. 1 PublicationAdd BLAST87
Alternative sequenceiVSP_053056671P → L in isoform 2. 2 Publications1
Alternative sequenceiVSP_053057672 – 714Missing in isoform 2. 2 PublicationsAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014568 mRNA Translation: BAB29433.1
AK042581 mRNA Translation: BAC31300.1
AK046761 mRNA Translation: BAC32857.1
AK050948 mRNA Translation: BAC34472.1
AK157398 mRNA Translation: BAE34079.1
AL611927 Genomic DNA Translation: CAM24579.1
AL611927 Genomic DNA Translation: CAM24580.1
CH466594 Genomic DNA Translation: EDL14922.1
BC024877 mRNA Translation: AAH24877.1 Sequence problems.
BC125432 mRNA Translation: AAI25433.1
BC132099 mRNA Translation: AAI32100.1
CCDSiCCDS18986.1 [Q3TZX8-2]
CCDS51389.1 [Q3TZX8-1]
RefSeqiNP_001153071.1, NM_001159599.2 [Q3TZX8-1]
NP_083003.2, NM_028727.3 [Q3TZX8-2]
UniGeneiMm.275803

Genome annotation databases

EnsembliENSMUST00000084116; ENSMUSP00000081133; ENSMUSG00000028948 [Q3TZX8-1]
ENSMUST00000103197; ENSMUSP00000099486; ENSMUSG00000028948 [Q3TZX8-2]
GeneIDi74035
KEGGimmu:74035
UCSCiuc008vzc.3 mouse [Q3TZX8-3]
uc008vzd.3 mouse [Q3TZX8-1]
uc008vzf.3 mouse [Q3TZX8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014568 mRNA Translation: BAB29433.1
AK042581 mRNA Translation: BAC31300.1
AK046761 mRNA Translation: BAC32857.1
AK050948 mRNA Translation: BAC34472.1
AK157398 mRNA Translation: BAE34079.1
AL611927 Genomic DNA Translation: CAM24579.1
AL611927 Genomic DNA Translation: CAM24580.1
CH466594 Genomic DNA Translation: EDL14922.1
BC024877 mRNA Translation: AAH24877.1 Sequence problems.
BC125432 mRNA Translation: AAI25433.1
BC132099 mRNA Translation: AAI32100.1
CCDSiCCDS18986.1 [Q3TZX8-2]
CCDS51389.1 [Q3TZX8-1]
RefSeqiNP_001153071.1, NM_001159599.2 [Q3TZX8-1]
NP_083003.2, NM_028727.3 [Q3TZX8-2]
UniGeneiMm.275803

3D structure databases

ProteinModelPortaliQ3TZX8
SMRiQ3TZX8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216440, 1 interactor
IntActiQ3TZX8, 3 interactors
MINTiQ3TZX8
STRINGi10090.ENSMUSP00000081133

PTM databases

iPTMnetiQ3TZX8
PhosphoSitePlusiQ3TZX8

Proteomic databases

EPDiQ3TZX8
MaxQBiQ3TZX8
PaxDbiQ3TZX8
PeptideAtlasiQ3TZX8
PRIDEiQ3TZX8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084116; ENSMUSP00000081133; ENSMUSG00000028948 [Q3TZX8-1]
ENSMUST00000103197; ENSMUSP00000099486; ENSMUSG00000028948 [Q3TZX8-2]
GeneIDi74035
KEGGimmu:74035
UCSCiuc008vzc.3 mouse [Q3TZX8-3]
uc008vzd.3 mouse [Q3TZX8-1]
uc008vzf.3 mouse [Q3TZX8-2]

Organism-specific databases

CTDi79707
MGIiMGI:1921285 Nol9

Phylogenomic databases

eggNOGiKOG2750 Eukaryota
COG1341 LUCA
GeneTreeiENSGT00390000012406
HOVERGENiHBG058105
InParanoidiQ3TZX8
KOiK06947
OMAiMDGLYTK
OrthoDBiEOG091G0BNE
PhylomeDBiQ3TZX8
TreeFamiTF313802

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

PROiPR:Q3TZX8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028948 Expressed in 227 organ(s), highest expression level in lumbar subsegment of spinal cord
ExpressionAtlasiQ3TZX8 baseline and differential
GenevisibleiQ3TZX8 MM

Family and domain databases

InterProiView protein in InterPro
IPR032319 CLP1_P
PfamiView protein in Pfam
PF16575 CLP1_P, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOL9_MOUSE
AccessioniPrimary (citable) accession number: Q3TZX8
Secondary accession number(s): A0PJC7
, B1AS65, Q05A31, Q8BQE1, Q8C8L5, Q8C9A8, Q9D678
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 11, 2005
Last modified: November 7, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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