UniProtKB - Q3TZ32 (Q3TZ32_MOUSE)
Protein
Alanine--tRNA ligase
Gene
Aars
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.UniRule annotation
Catalytic activityi
- EC:6.1.1.7UniRule annotationARBA annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 605 | ZincUniRule annotation | 1 | |
Metal bindingi | 609 | ZincUniRule annotation | 1 | |
Metal bindingi | 723 | ZincUniRule annotation | 1 | |
Metal bindingi | 727 | ZincUniRule annotation | 1 |
GO - Molecular functioni
- alanine-tRNA ligase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-UniRule
- tRNA binding Source: UniProtKB-KW
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- alanyl-tRNA aminoacylation Source: InterPro
Keywordsi
Molecular function | Aminoacyl-tRNA synthetaseUniRule annotationARBA annotation, Ligase, RNA-binding, tRNA-bindingUniRule annotationARBA annotation |
Biological process | Protein biosynthesisUniRule annotationARBA annotation |
Ligand | ATP-bindingUniRule annotationARBA annotation, Metal-bindingUniRule annotation, Nucleotide-binding, ZincUniRule annotation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Organism-specific databases
MGIi | MGI:2384560, Aars |
Subcellular locationi
Other locations
- cytoplasm Source: InterPro
PTM / Processingi
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineUniRule annotation | 1 |
Post-translational modificationi
ISGylated.UniRule annotation
Keywords - PTMi
AcetylationUniRule annotation, Ubl conjugationUniRule annotationProteomic databases
PeptideAtlasi | Q3TZ32 |
PRIDEi | Q3TZ32 |
PTM databases
SwissPalmi | Q3TZ32 |
Interactioni
Subunit structurei
Monomer.
Interacts with ANKRD16; the interaction is direct.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 766 | AA_TRNA_LIGASE_II_ALAInterPro annotationAdd BLAST | 762 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 760 – 780 | Sequence analysisAdd BLAST | 21 | |
Coiled coili | 812 – 832 | Sequence analysisAdd BLAST | 21 |
Domaini
Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation
Sequence similaritiesi
Keywords - Domaini
Coiled coilSequence analysisFamily and domain databases
HAMAPi | MF_00036_B, Ala_tRNA_synth_B, 1 hit |
InterProi | View protein in InterPro IPR002318, Ala-tRNA-lgiase_IIc IPR018162, Ala-tRNA-ligase_IIc_anticod-bd IPR018165, Ala-tRNA-synth_IIc_core IPR018164, Ala-tRNA-synth_IIc_N IPR023033, Ala_tRNA_ligase_euk/bac IPR003156, DHHA1_dom IPR018163, Thr/Ala-tRNA-synth_IIc_edit IPR009000, Transl_B-barrel_sf IPR012947, tRNA_SAD |
Pfami | View protein in Pfam PF02272, DHHA1, 1 hit PF01411, tRNA-synt_2c, 1 hit PF07973, tRNA_SAD, 1 hit |
PRINTSi | PR00980, TRNASYNTHALA |
SMARTi | View protein in SMART SM00863, tRNA_SAD, 1 hit |
SUPFAMi | SSF101353, SSF101353, 1 hit SSF50447, SSF50447, 1 hit SSF55186, SSF55186, 1 hit |
TIGRFAMsi | TIGR00344, alaS, 1 hit |
PROSITEi | View protein in PROSITE PS50860, AA_TRNA_LIGASE_II_ALA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q3TZ32-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDATLTAREI RERFINFFRR NEHTYVHSSA TIPLDDPTLL FANAGMNQFK
60 70 80 90 100
PIFLNTIDPS HPMAKLSRAA NTQKCIRAGG KHNDLDDVGK DVYHHTFFEM
110 120 130 140 150
LGSWSFGDYF KELACKMALE LLTQEFGIPV ERLYVTYLGG DEAAGLEPDL
160 170 180 190 200
ECRQIWQNLG LDEARILPGN MKDNFWEMGD TGPCGPCSEI HYDRIGGRDA
210 220 230 240 250
AHLVNQDDPN VLEIWNLVFI QYNRESDGVL KPLPKKSIDT GMGLERLVSV
260 270 280 290 300
LQNKMSNYDT DLFMPYFEAI QKGTGARPYT GKVGAEDADG IDMAYRVLAD
310 320 330 340 350
HARTITVALA DGGRPDNTGR GYVLRRILRR AVRYSHEKLN ASRGFFATLV
360 370 380 390 400
DVVVQSLGDA FPELKKDPDM VKDIINEEEV QFLKTLSRGR RILDRKIQSL
410 420 430 440 450
GDCKTIPGDT AWLLYDTYGF PVDLTGLIAE EKGLVVDMNG FEEERRLAQL
460 470 480 490 500
KSQGKGAGDE DLIMLDIYAI EELRAKGLEA TDDSPKYNYQ SDSSGSYVFE
510 520 530 540 550
CTVATVLALR REKMFVDEVV TGQECGVVLD KTCFYAEQGG QIYDEGYLVK
560 570 580 590 600
VDDSSEDKTE FTVKNAQVRG GYVLHIGTIY GNLKVGDQVR LFIDEPRRRP
610 620 630 640 650
VMSNHTATHI LNFALRSVLG EADQKGSLVA PDRLRFDFTA KGAMSTQQIK
660 670 680 690 700
KAEEIVNGMI EAAKPVYTQD CPLAAAKAIQ GLRAVFDETY PDPVRVVSIG
710 720 730 740 750
VPVSELLDDP CGPAGSLTSV EFCGGTHLRN SSHAGAFVIV TEEAIAKGIR
760 770 780 790 800
RIVAVTGAEA QKALRKSETL KKSLSAMEAK VKAQTAPNKD VQREIADLGE
810 820 830 840 850
ALATAVIPQW QKDEQRETLK SLKKVMDDLD RASKADVQKR VLEKTKQLID
860 870 880 890 900
SNPNQPLVIL EMESGASAKA LNEALKLFKT HSPQTSAMLF TVDNEAGKIT
910 920 930 940 950
CLCQVPQNAA NRGLKASEWV QQVSGLMDGK GGGKDMSAQA TGKNVGCLQE
960
ALQLATSFAQ LRLGDVKN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK158139 mRNA Translation: BAE34378.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK158139 mRNA Translation: BAE34378.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
PTM databases
SwissPalmi | Q3TZ32 |
Proteomic databases
PeptideAtlasi | Q3TZ32 |
PRIDEi | Q3TZ32 |
Organism-specific databases
MGIi | MGI:2384560, Aars |
Miscellaneous databases
ChiTaRSi | Aars, mouse |
SOURCEi | Search... |
Family and domain databases
HAMAPi | MF_00036_B, Ala_tRNA_synth_B, 1 hit |
InterProi | View protein in InterPro IPR002318, Ala-tRNA-lgiase_IIc IPR018162, Ala-tRNA-ligase_IIc_anticod-bd IPR018165, Ala-tRNA-synth_IIc_core IPR018164, Ala-tRNA-synth_IIc_N IPR023033, Ala_tRNA_ligase_euk/bac IPR003156, DHHA1_dom IPR018163, Thr/Ala-tRNA-synth_IIc_edit IPR009000, Transl_B-barrel_sf IPR012947, tRNA_SAD |
Pfami | View protein in Pfam PF02272, DHHA1, 1 hit PF01411, tRNA-synt_2c, 1 hit PF07973, tRNA_SAD, 1 hit |
PRINTSi | PR00980, TRNASYNTHALA |
SMARTi | View protein in SMART SM00863, tRNA_SAD, 1 hit |
SUPFAMi | SSF101353, SSF101353, 1 hit SSF50447, SSF50447, 1 hit SSF55186, SSF55186, 1 hit |
TIGRFAMsi | TIGR00344, alaS, 1 hit |
PROSITEi | View protein in PROSITE PS50860, AA_TRNA_LIGASE_II_ALA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q3TZ32_MOUSE | |
Accessioni | Q3TZ32Primary (citable) accession number: Q3TZ32 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | October 11, 2005 |
Last sequence update: | October 11, 2005 | |
Last modified: | August 12, 2020 | |
This is version 100 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |