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Protein

Deoxyhypusine synthase

Gene

Dhps

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

NAD+By similarity

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei137NADBy similarity1
Binding sitei238NADBy similarity1
Binding sitei243SpermidineBy similarity1
Binding sitei283NAD; via amide nitrogenBy similarity1
Binding sitei288SpermidineBy similarity1
Active sitei329NucleophileBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi105 – 109NADBy similarity5
Nucleotide bindingi131 – 133NADBy similarity3
Nucleotide bindingi308 – 309NADBy similarity2
Nucleotide bindingi342 – 343NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processHypusine biosynthesis
LigandNAD

Enzyme and pathway databases

ReactomeiR-MMU-204626 Hypusine synthesis from eIF5A-lysine
UniPathwayi
UPA00354

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Short name:
DHS
Gene namesi
Name:Dhps
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2683592 Dhps

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003149441 – 369Deoxyhypusine synthaseAdd BLAST369

Proteomic databases

EPDiQ3TXU5
MaxQBiQ3TXU5
PaxDbiQ3TXU5
PeptideAtlasiQ3TXU5
PRIDEiQ3TXU5

PTM databases

iPTMnetiQ3TXU5
PhosphoSitePlusiQ3TXU5

Expressioni

Gene expression databases

BgeeiENSMUSG00000060038 Expressed in 258 organ(s), highest expression level in supraoptic nucleus
CleanExiMM_DHPS
ExpressionAtlasiQ3TXU5 baseline and differential
GenevisibleiQ3TXU5 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077733

Structurei

3D structure databases

ProteinModelPortaliQ3TXU5
SMRiQ3TXU5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 137Spermidine bindingBy similarity2
Regioni314 – 316Spermidine bindingBy similarity3
Regioni323 – 329Spermidine bindingBy similarity7

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

eggNOGiKOG2924 Eukaryota
COG1899 LUCA
GeneTreeiENSGT00390000008063
HOGENOMiHOG000228838
HOVERGENiHBG000852
InParanoidiQ3TXU5
KOiK00809
OMAiGWEDSTM
OrthoDBiEOG091G09FV
TreeFamiTF300625

Family and domain databases

Gene3Di3.40.910.10, 1 hit
InterProiView protein in InterPro
IPR002773 Deoxyhypusine_synthase
IPR036982 Deoxyhypusine_synthase_sf
IPR029035 DHS-like_NAD/FAD-binding_dom
PANTHERiPTHR11703 PTHR11703, 1 hit
PfamiView protein in Pfam
PF01916 DS, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
TIGRFAMsiTIGR00321 dhys, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q3TXU5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEGTPPGAAP SSALAAVLKH SSALPPESAQ VQGYDFNRGV DYHALLDAYR
60 70 80 90 100
TTGFQATNFG RAVQQVNAMI EKKLEPLAVD EDHHADLTQS RRPLTGCTIF
110 120 130 140 150
LGYTSNLISS GIRETIRYLV QHNMVDVLVT TAGGVEEDLI KCLAPTYLGE
160 170 180 190 200
FSLRGKELRE SGINRIGNLL VPNDNYCKFE DWLMPILDQM VLEQNTEGVK
210 220 230 240 250
WTPSKMISRL GKEINNPDSV YYWAHKNHIP VLSPALTDGS LGDMIFFHSY
260 270 280 290 300
KNPGLVLDIV EDLRLINTQA IFAKRSGMII LGGGVVKHHI ANANLMRNGA
310 320 330 340 350
DYAVYINTAQ EFDGSDSGAR PDEAVSWGKI RMDAQPVKVY ADASLVFPLL
360
VAETFAQKAD AFRAEKNED
Length:369
Mass (Da):40,642
Last modified:July 27, 2011 - v2
Checksum:i22B835EEE4E9DFAC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6W7I2F6W7I2_MOUSE
Deoxyhypusine synthase
Dhps
149Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti340Y → C in BAE34820 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159104 mRNA Translation: BAE34820.1
AC163703 Genomic DNA No translation available.
CCDSiCCDS22492.1
RefSeqiNP_001034603.1, NM_001039514.1
UniGeneiMm.193086
Mm.456030

Genome annotation databases

EnsembliENSMUST00000078665; ENSMUSP00000077733; ENSMUSG00000060038
GeneIDi330817
KEGGimmu:330817
UCSCiuc009mpf.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159104 mRNA Translation: BAE34820.1
AC163703 Genomic DNA No translation available.
CCDSiCCDS22492.1
RefSeqiNP_001034603.1, NM_001039514.1
UniGeneiMm.193086
Mm.456030

3D structure databases

ProteinModelPortaliQ3TXU5
SMRiQ3TXU5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077733

PTM databases

iPTMnetiQ3TXU5
PhosphoSitePlusiQ3TXU5

Proteomic databases

EPDiQ3TXU5
MaxQBiQ3TXU5
PaxDbiQ3TXU5
PeptideAtlasiQ3TXU5
PRIDEiQ3TXU5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078665; ENSMUSP00000077733; ENSMUSG00000060038
GeneIDi330817
KEGGimmu:330817
UCSCiuc009mpf.1 mouse

Organism-specific databases

CTDi1725
MGIiMGI:2683592 Dhps

Phylogenomic databases

eggNOGiKOG2924 Eukaryota
COG1899 LUCA
GeneTreeiENSGT00390000008063
HOGENOMiHOG000228838
HOVERGENiHBG000852
InParanoidiQ3TXU5
KOiK00809
OMAiGWEDSTM
OrthoDBiEOG091G09FV
TreeFamiTF300625

Enzyme and pathway databases

UniPathwayi
UPA00354

ReactomeiR-MMU-204626 Hypusine synthesis from eIF5A-lysine

Miscellaneous databases

ChiTaRSiDhps mouse
PROiPR:Q3TXU5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000060038 Expressed in 258 organ(s), highest expression level in supraoptic nucleus
CleanExiMM_DHPS
ExpressionAtlasiQ3TXU5 baseline and differential
GenevisibleiQ3TXU5 MM

Family and domain databases

Gene3Di3.40.910.10, 1 hit
InterProiView protein in InterPro
IPR002773 Deoxyhypusine_synthase
IPR036982 Deoxyhypusine_synthase_sf
IPR029035 DHS-like_NAD/FAD-binding_dom
PANTHERiPTHR11703 PTHR11703, 1 hit
PfamiView protein in Pfam
PF01916 DS, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
TIGRFAMsiTIGR00321 dhys, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDHYS_MOUSE
AccessioniPrimary (citable) accession number: Q3TXU5
Secondary accession number(s): E9QM48
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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