UniProtKB - Q3TWW8 (SRSF6_MOUSE)
Protein
Serine/arginine-rich splicing factor 6
Gene
Srsf6
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in constitutive splicing and modulates the selection of alternative splice sites. Plays a role in the alternative splicing of MAPT/Tau exon 10. Binds to alternative exons of TNC pre-mRNA and promotes the expression of alternatively spliced TNC. Plays a role in wound healing and in the regulation of keratinocyte differentiation and proliferation via its role in alternative splicing (By similarity).By similarity
GO - Molecular functioni
- mRNA binding Source: GO_Central
- pre-mRNA binding Source: UniProtKB
- RNA binding Source: UniProtKB
GO - Biological processi
- alternative mRNA splicing, via spliceosome Source: UniProtKB
- mRNA cis splicing, via spliceosome Source: GO_Central
- mRNA splice site selection Source: UniProtKB
- negative regulation of cell death Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of keratinocyte differentiation Source: UniProtKB
- negative regulation of mRNA splicing, via spliceosome Source: MGI
- negative regulation of type B pancreatic cell apoptotic process Source: MGI
- positive regulation of epithelial cell proliferation involved in lung morphogenesis Source: MGI
- regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
- regulation of keratinocyte proliferation Source: UniProtKB
- regulation of wound healing Source: UniProtKB
- response to insulin Source: Ensembl
Keywordsi
Molecular function | Repressor, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
Reactomei | R-MMU-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72165, mRNA Splicing - Minor Pathway R-MMU-72187, mRNA 3'-end processing R-MMU-73856, RNA Polymerase II Transcription Termination |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/arginine-rich splicing factor 6Alternative name(s): Pre-mRNA-splicing factor SRP55 Splicing factor, arginine/serine-rich 6 |
Gene namesi | Name:Srsf6 Synonyms:Sfrs6, Srp55 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915246, Srsf6 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
- Nucleus speckle By similarity
Nucleus
- nuclear speck Source: UniProtKB
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000426095 | 1 – 339 | Serine/arginine-rich splicing factor 6Add BLAST | 339 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 45 | PhosphoserineCombined sources | 1 | |
Modified residuei | 81 | PhosphoserineBy similarity | 1 | |
Modified residuei | 84 | PhosphoserineBy similarity | 1 | |
Modified residuei | 165 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 182 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 297 | PhosphoserineBy similarity | 1 | |
Modified residuei | 299 | PhosphoserineBy similarity | 1 | |
Modified residuei | 303 | Phosphoserine; by DYRK1ABy similarity | 1 | |
Modified residuei | 314 | PhosphoserineCombined sources | 1 | |
Modified residuei | 316 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Extensively phosphorylated on serine residues in the RS domain. Phosphorylated by DYRK1A, probably in the RS domain. Phosphorylation by DYRK1A modulates alternative splice site selection and inhibits the expression of MAPT/Tau exon 10 (By similarity).By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q3TWW8 |
jPOSTi | Q3TWW8 |
MaxQBi | Q3TWW8 |
PaxDbi | Q3TWW8 |
PeptideAtlasi | Q3TWW8 |
PRIDEi | Q3TWW8 |
PTM databases
iPTMneti | Q3TWW8 |
PhosphoSitePlusi | Q3TWW8 |
SwissPalmi | Q3TWW8 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000016921, Expressed in metanephric loop of Henle and 319 other tissues |
ExpressionAtlasi | Q3TWW8, baseline and differential |
Genevisiblei | Q3TWW8, MM |
Interactioni
Subunit structurei
Binds SREK1/SFRS12.
Interacts with DYRK1A (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 212588, 3 interactors |
IntActi | Q3TWW8, 3 interactors |
STRINGi | 10090.ENSMUSP00000119065 |
Miscellaneous databases
RNActi | Q3TWW8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 72 | RRM 1PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 110 – 183 | RRM 2PROSITE-ProRule annotationAdd BLAST | 74 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 87 – 90 | Gly-rich (hinge region) | 4 | |
Compositional biasi | 184 – 338 | Arg/Ser-rich (RS domain)Add BLAST | 155 |
Sequence similaritiesi
Belongs to the splicing factor SR family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0106, Eukaryota |
GeneTreei | ENSGT00940000155448 |
HOGENOMi | CLU_012062_34_2_1 |
InParanoidi | Q3TWW8 |
OMAi | YSDHEER |
OrthoDBi | 1315388at2759 |
PhylomeDBi | Q3TWW8 |
TreeFami | TF351335 |
Family and domain databases
CDDi | cd12596, RRM1_SRSF6, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR029541, SRSF6 IPR034511, SRSF6_RRM1 |
PANTHERi | PTHR23147:SF82, PTHR23147:SF82, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q3TWW8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPRVYIGRLS YNVREKDIQR FFSGYGRLLE IDLKNGYGFV EFEDSRDADD
60 70 80 90 100
AVYELNSKEL CGERVIVEHA RGPRRDRDGY SYGSRSGGGG YSSRRTSGRD
110 120 130 140 150
KYGPPVRTEY RLIVENLSSR CSWQDLKDFM RQAGEVTYAD AHKERTNEGV
160 170 180 190 200
IEFRSYSDMK RALDKLDGTE INGRNIRLIE DKPRTSHRRS YSGSRSRSRS
210 220 230 240 250
RRRSRSRSRR SSRSRSRSIS KSRSRSRSRS KGRSRSRSKG RKSRSKSKSK
260 270 280 290 300
PKSDRGSHSH SRSRSKDKYG KSRSRSRSRS PKENGKGDIK SKSRSRSQSR
310 320 330
SHSPLPAPPS KARSMSPPPK RASRSRSRSR SRSRSSSRD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0A6YXX6 | A0A0A6YXX6_MOUSE | Serine/arginine-rich-splicing facto... | Srsf6 | 135 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 201 | R → K in BAB23599 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 230 | S → G in BAE27050 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 299 | S → P in BAE27050 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 319 | P → T in AAH12039 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004831 mRNA Translation: BAB23599.1 AK133872 mRNA Translation: BAE21902.1 AK146293 mRNA Translation: BAE27050.1 AK159519 mRNA Translation: BAE35148.1 AL590418 Genomic DNA No translation available. AL606473 Genomic DNA No translation available. CH466551 Genomic DNA Translation: EDL06308.1 BC012039 mRNA Translation: AAH12039.1 |
CCDSi | CCDS17002.1 |
RefSeqi | NP_080775.3, NM_026499.4 |
Genome annotation databases
Ensembli | ENSMUST00000130411; ENSMUSP00000119065; ENSMUSG00000016921 |
GeneIDi | 67996 |
KEGGi | mmu:67996 |
UCSCi | uc008nsc.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004831 mRNA Translation: BAB23599.1 AK133872 mRNA Translation: BAE21902.1 AK146293 mRNA Translation: BAE27050.1 AK159519 mRNA Translation: BAE35148.1 AL590418 Genomic DNA No translation available. AL606473 Genomic DNA No translation available. CH466551 Genomic DNA Translation: EDL06308.1 BC012039 mRNA Translation: AAH12039.1 |
CCDSi | CCDS17002.1 |
RefSeqi | NP_080775.3, NM_026499.4 |
3D structure databases
SMRi | Q3TWW8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 212588, 3 interactors |
IntActi | Q3TWW8, 3 interactors |
STRINGi | 10090.ENSMUSP00000119065 |
PTM databases
iPTMneti | Q3TWW8 |
PhosphoSitePlusi | Q3TWW8 |
SwissPalmi | Q3TWW8 |
Proteomic databases
EPDi | Q3TWW8 |
jPOSTi | Q3TWW8 |
MaxQBi | Q3TWW8 |
PaxDbi | Q3TWW8 |
PeptideAtlasi | Q3TWW8 |
PRIDEi | Q3TWW8 |
Protocols and materials databases
Antibodypediai | 27122, 103 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000130411; ENSMUSP00000119065; ENSMUSG00000016921 |
GeneIDi | 67996 |
KEGGi | mmu:67996 |
UCSCi | uc008nsc.2, mouse |
Organism-specific databases
CTDi | 6431 |
MGIi | MGI:1915246, Srsf6 |
Phylogenomic databases
eggNOGi | KOG0106, Eukaryota |
GeneTreei | ENSGT00940000155448 |
HOGENOMi | CLU_012062_34_2_1 |
InParanoidi | Q3TWW8 |
OMAi | YSDHEER |
OrthoDBi | 1315388at2759 |
PhylomeDBi | Q3TWW8 |
TreeFami | TF351335 |
Enzyme and pathway databases
Reactomei | R-MMU-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72165, mRNA Splicing - Minor Pathway R-MMU-72187, mRNA 3'-end processing R-MMU-73856, RNA Polymerase II Transcription Termination |
Miscellaneous databases
BioGRID-ORCSi | 67996, 1 hit in 17 CRISPR screens |
ChiTaRSi | Srsf6, mouse |
PROi | PR:Q3TWW8 |
RNActi | Q3TWW8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000016921, Expressed in metanephric loop of Henle and 319 other tissues |
ExpressionAtlasi | Q3TWW8, baseline and differential |
Genevisiblei | Q3TWW8, MM |
Family and domain databases
CDDi | cd12596, RRM1_SRSF6, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR029541, SRSF6 IPR034511, SRSF6_RRM1 |
PANTHERi | PTHR23147:SF82, PTHR23147:SF82, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SRSF6_MOUSE | |
Accessioni | Q3TWW8Primary (citable) accession number: Q3TWW8 Secondary accession number(s): Q3UJV5, Q921K3, Q9DBP1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | October 11, 2005 | |
Last modified: | February 10, 2021 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families