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UniProtKB - Q3TTY0 (PLB1_MOUSE)

Protein

Phospholipase B1, membrane-associated

Gene

Plb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Activity regulationi

Inhibited by diisopropyl fluorophosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei404NucleophileBy similarity1
Binding sitei478Substrate; via amide nitrogenBy similarity1
Binding sitei517SubstrateBy similarity1
Active sitei1006By similarity1
Active sitei1351By similarity1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • lipid catabolic process Source: UniProtKB-KW
  • phospholipid metabolic process Source: MGI
  • positive regulation of acrosome reaction Source: MGI
  • retinol metabolic process Source: MGI
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.5 3474
ReactomeiR-MMU-1482788 Acyl chain remodelling of PC
R-MMU-975634 Retinoid metabolism and transport

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:Plb1
Synonyms:Plb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1922406 Plb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 1422ExtracellularSequence analysisAdd BLAST1399
Transmembranei1423 – 1443HelicalSequence analysisAdd BLAST21
Topological domaini1444 – 1478CytoplasmicSequence analysisAdd BLAST35

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000032438524 – 1478Phospholipase B1, membrane-associatedAdd BLAST1455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi525N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi626N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi637N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi801N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi830N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1227N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ3TTY0
PaxDbiQ3TTY0
PRIDEiQ3TTY0

PTM databases

iPTMnetiQ3TTY0
PhosphoSitePlusiQ3TTY0

Expressioni

Gene expression databases

BgeeiENSMUSG00000029134 Expressed in 66 organ(s), highest expression level in intestine
CleanExiMM_PLB1
GenevisibleiQ3TTY0 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000098927

Structurei

3D structure databases

ProteinModelPortaliQ3TTY0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati43 – 3511Add BLAST309
Repeati366 – 7112Add BLAST346
Repeati712 – 10583Add BLAST347
Repeati1068 – 14074Add BLAST340

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 14074 X 308-326 AA approximate repeatsAdd BLAST1365
Regioni1408 – 1450Necessary for membrane localizationBy similarityAdd BLAST43

Domaini

Repeat 2 contains the catalytic domain.By similarity

Sequence similaritiesi

Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3670 Eukaryota
ENOG410ZM9P LUCA
GeneTreeiENSGT00530000063883
HOGENOMiHOG000115574
HOVERGENiHBG108263
InParanoidiQ3TTY0
KOiK14621
OMAiENPYLFT
OrthoDBiEOG091G00VL
PhylomeDBiQ3TTY0
TreeFamiTF314942

Family and domain databases

CDDicd01824 Phospholipase_B_like, 4 hits
Gene3Di3.40.50.1110, 2 hits
InterProiView protein in InterPro
IPR001087 GDSL
IPR008265 Lipase_GDSL_AS
IPR035547 Phospholipase_B
IPR038885 PLB1
IPR036514 SGNH_hydro_sf
PANTHERiPTHR21325 PTHR21325, 4 hits
PfamiView protein in Pfam
PF00657 Lipase_GDSL, 3 hits
PROSITEiView protein in PROSITE
PS01098 LIPASE_GDSL_SER, 2 hits

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q3TTY0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELYPGVSPV GLLLLLLLGQ GPSQIHGSSG ENTLAWQSQQ VFWTLKNFPF 
        60         70         80         90        100
PCKPKKLELS VLSESVHSLR PSDIKLVAAI GNPEIPLAPG SGTINMEKPQ 
       110        120        130        140        150
SIKNQPQDVC MGIMTVLSDI IRHFSPSVLM PTCSPGKGTA VHTTAEDLWI 
       160        170        180        190        200
QAKELVRRLK DNPQLDFEKD WKLITVFFSN TSQCHLCPSA QQKSHLMRHM 
       210        220        230        240        250
EMLWGVLDYL HHEVPRAFVN LVDLSEVLAM DLQHQETGFS PAPEVCKCTE 
       260        270        280        290        300
TTTLSKAVMQ WSYQEAWEDL LASSKFNKHE TFAVVFQPFF DEIEPPLKRS 
       310        320        330        340        350
SPQDPTTLAL RIWNSMMEPV GQKDGLLNTA ERKTMKCPSE ESPYLFTYKN 
       360        370        380        390        400
SNYQARRLKP ITKLQMKEGS EFTCPDKNPS NSIPTTVHSL RPADIKIIGA 
       410        420        430        440        450
LGDSLTAGNG AGASPWNILD VLTEYRGLSW SVGGDETIKT VTTLPNILRE 
       460        470        480        490        500
FNPSLKGFSV GTGKESTSRA SFNQAVAGAK SDGLAGQARK LVDLMKADKT 
       510        520        530        540        550
INFQEDWKII TVFIGGNDLC ASCSNSTRFS PQNFIDNIKN ALDILHAEVP 
       560        570        580        590        600
RAFVNMAMVM EITPLRELFN EPTVSCPRNI LSRLCPCVLG LGDNSEELSS 
       610        620        630        640        650
LVQRNRDYQK KTEELINSGR YDTRDNFTVV VQPLFENVSM PRTPEGVPDK 
       660        670        680        690        700
SFFAPDCFHF NAKTHARSAI ALWKNMLEPV GHKTRHNNFE IKAPIVCPNQ 
       710        720        730        740        750
ASPFLSTTKN SNLGNGTWMV CEERAPSASP PTSVHTLRPA DIQVVAALGD 
       760        770        780        790        800
SLTAGNGISS QEGNLTDVST QYRGLSYSAG GDKTLENVTT LPNILRKFNG 
       810        820        830        840        850
NLTGYSVGTG DSSSANAFLN QAVPGAKAEN LTSQVRTLVQ KMKSDNRVNF 
       860        870        880        890        900
NRDWKVITVM IGASDLCDFC TDSNHYSAAN FFDHLQNALD ILHKEVPRAL 
       910        920        930        940        950
VNLVDFINPS IIREVFLKNP DKCPVNQSSV LCNCVLTPRK DSYELARLEA 
       960        970        980        990       1000
FTKSYQSSML QLVESGRYDT REDFSVVLQP FLLNTKLPVL ENGKPDTSFF 
      1010       1020       1030       1040       1050
APDCIHLNQK FHTQLARALW ANMLEPLGKK TDTLDPKGHI SLACPTKDQP 
      1060       1070       1080       1090       1100
FLRTFRNSNY KYPTKPAIEN WGSDFLCTEK SPSSQVPTSV HELRPADIKV 
      1110       1120       1130       1140       1150
VAAMGDFLTT ATGARPSGYK RLATPWRGLS WSIGGDGKLE THTTLPNILK 
      1160       1170       1180       1190       1200
KFNPSITGFS TGTLDNKAGL NVAEEGARAQ DMPAQAKTLV KKMKSTPTIN 
      1210       1220       1230       1240       1250
LQEDWKLITL LIGNNDLCLY CENPEDNSTK EYVKYIQQAL DILYEELPRV 
      1260       1270       1280       1290       1300
FINVVEVMEL AGLHHVQGGK CAMPLAVQKN CSCLRHSQNL TAMQELKKLN 
      1310       1320       1330       1340       1350
WNLQSGISEL SYWHRYMERE DFAVTVQPFF RNTFIPLNER EGLDLTFFSE 
      1360       1370       1380       1390       1400
DCFYFSDRGH AEMAIALWNN MLEPVGWKTS SNNFIYNRTK LKCPSPERPF 
      1410       1420       1430       1440       1450
LYTLRNSQLL PDKAEEPSNA LYWAVPVAAI GGLAVGILGV MLWRTVKPVQ 
      1460       1470 
QEEEEEDTLP NTSVTQDAVS EKRLKAGN
Length:1,478
Mass (Da):164,541
Last modified:March 18, 2008 - v2
Checksum:iB5361AED29BA49E6
GO
Isoform 2 (identifier: Q3TTY0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     778-778: S → R
     779-1478: Missing.

Note: No experimental confirmation available.
Show »
Length:778
Mass (Da):86,140
Checksum:i2E08238E090D67AA
GO
Isoform 3 (identifier: Q3TTY0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     929-929: S → R
     930-1478: Missing.

Note: No experimental confirmation available.
Show »
Length:929
Mass (Da):102,708
Checksum:i8AA3E12EB262376A
GO
Isoform 4 (identifier: Q3TTY0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     387-394: VHSLRPAD → GTWLSCSV
     395-1478: Missing.

Note: No experimental confirmation available.
Show »
Length:394
Mass (Da):44,290
Checksum:iE6DB3EC17E8F0888
GO

Sequence cautioni

The sequence AAI19078 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032233387 – 394VHSLRPAD → GTWLSCSV in isoform 4. 1 Publication8
Alternative sequenceiVSP_032234395 – 1478Missing in isoform 4. 1 PublicationAdd BLAST1084
Alternative sequenceiVSP_032235778S → R in isoform 2. 1 Publication1
Alternative sequenceiVSP_032236779 – 1478Missing in isoform 2. 1 PublicationAdd BLAST700
Alternative sequenceiVSP_032237929S → R in isoform 3. 1 Publication1
Alternative sequenceiVSP_032238930 – 1478Missing in isoform 3. 1 PublicationAdd BLAST549

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK015993 mRNA Translation: BAB30072.1
AK161095 mRNA Translation: BAE36193.1
AC102372 Genomic DNA No translation available.
AC111030 Genomic DNA No translation available.
BC119077 mRNA Translation: AAI19078.1 Different initiation.
CCDSiCCDS39060.1 [Q3TTY0-1]
RefSeqiNP_001074876.1, NM_001081407.1 [Q3TTY0-1]
NP_084348.1, NM_030072.1
XP_006504110.1, XM_006504047.2 [Q3TTY0-1]
UniGeneiMm.160067

Genome annotation databases

EnsembliENSMUST00000101376; ENSMUSP00000098927; ENSMUSG00000029134 [Q3TTY0-1]
ENSMUST00000202201; ENSMUSP00000144401; ENSMUSG00000029134 [Q3TTY0-2]
ENSMUST00000202220; ENSMUSP00000144040; ENSMUSG00000029134 [Q3TTY0-1]
GeneIDi665270
KEGGimmu:665270
UCSCiuc008wzk.1 mouse [Q3TTY0-4]
uc008wzl.1 mouse [Q3TTY0-3]
uc008wzm.1 mouse [Q3TTY0-2]
uc008wzn.1 mouse [Q3TTY0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK015993 mRNA Translation: BAB30072.1
AK161095 mRNA Translation: BAE36193.1
AC102372 Genomic DNA No translation available.
AC111030 Genomic DNA No translation available.
BC119077 mRNA Translation: AAI19078.1 Different initiation.
CCDSiCCDS39060.1 [Q3TTY0-1]
RefSeqiNP_001074876.1, NM_001081407.1 [Q3TTY0-1]
NP_084348.1, NM_030072.1
XP_006504110.1, XM_006504047.2 [Q3TTY0-1]
UniGeneiMm.160067

3D structure databases

ProteinModelPortaliQ3TTY0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000098927

PTM databases

iPTMnetiQ3TTY0
PhosphoSitePlusiQ3TTY0

Proteomic databases

MaxQBiQ3TTY0
PaxDbiQ3TTY0
PRIDEiQ3TTY0

Protocols and materials databases

DNASUi665270
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000101376; ENSMUSP00000098927; ENSMUSG00000029134 [Q3TTY0-1]
ENSMUST00000202201; ENSMUSP00000144401; ENSMUSG00000029134 [Q3TTY0-2]
ENSMUST00000202220; ENSMUSP00000144040; ENSMUSG00000029134 [Q3TTY0-1]
GeneIDi665270
KEGGimmu:665270
UCSCiuc008wzk.1 mouse [Q3TTY0-4]
uc008wzl.1 mouse [Q3TTY0-3]
uc008wzm.1 mouse [Q3TTY0-2]
uc008wzn.1 mouse [Q3TTY0-1]

Organism-specific databases

CTDi151056
MGIiMGI:1922406 Plb1

Phylogenomic databases

eggNOGiKOG3670 Eukaryota
ENOG410ZM9P LUCA
GeneTreeiENSGT00530000063883
HOGENOMiHOG000115574
HOVERGENiHBG108263
InParanoidiQ3TTY0
KOiK14621
OMAiENPYLFT
OrthoDBiEOG091G00VL
PhylomeDBiQ3TTY0
TreeFamiTF314942

Enzyme and pathway databases

BRENDAi3.1.1.5 3474
ReactomeiR-MMU-1482788 Acyl chain remodelling of PC
R-MMU-975634 Retinoid metabolism and transport

Miscellaneous databases

PROiPR:Q3TTY0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029134 Expressed in 66 organ(s), highest expression level in intestine
CleanExiMM_PLB1
GenevisibleiQ3TTY0 MM

Family and domain databases

CDDicd01824 Phospholipase_B_like, 4 hits
Gene3Di3.40.50.1110, 2 hits
InterProiView protein in InterPro
IPR001087 GDSL
IPR008265 Lipase_GDSL_AS
IPR035547 Phospholipase_B
IPR038885 PLB1
IPR036514 SGNH_hydro_sf
PANTHERiPTHR21325 PTHR21325, 4 hits
PfamiView protein in Pfam
PF00657 Lipase_GDSL, 3 hits
PROSITEiView protein in PROSITE
PS01098 LIPASE_GDSL_SER, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPLB1_MOUSE
AccessioniPrimary (citable) accession number: Q3TTY0
Secondary accession number(s): Q0VEX7, Q9D4Y6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: November 7, 2018
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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