UniProtKB - Q3TRM4 (PLPL6_MOUSE)
Protein
Patatin-like phospholipase domain-containing protein 6
Gene
Pnpla6
Organism
Mus musculus (Mouse)
Status
Functioni
Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho) (PubMed:18086666) (Probable). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Catalyzes the hydrolysis of several naturally occurring membrane-associated lipids. Hydrolyzes lysophospholipids and monoacylglycerols, preferring the 1-acyl to the 2-acyl isomer. Does not catalyze hydrolysis of di- or triacylglycerols or fatty acid amides (By similarity).By similarity1 Publication1 Publication
Miscellaneous
Specific chemical modification by some organophosphorus (OP) compounds leads to distal axonopathy in humans and chicken (Probable). The effects of these compounds in mice appear to be less severe (Probable). Mice treated with 1 mg/kg/body weight of ethyl octylphosphonofluoridate (EOPF) have elevated motor activity in the long term (PubMed:12640454). Higher doses result in increased mortality (PubMed:12640454).1 Publication1 Publication
Catalytic activityi
- a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H+ + sn-glycerol 3-phosphocholine1 Publication1 PublicationEC:3.1.1.51 Publication1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H+ + hexadecanoate + sn-glycerol 3-phosphocholine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-sn-glycero-3-phosphate + H2O = H+ + hexadecanoate + sn-glycerol 3-phosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- a 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + H+ + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
Inhibited by a series a OPs such as mipafox (MPX), phenyl saligenin phosphate (PSP), phenyl dipentyl phosphinate (PDPP), diisopropyl fluorophosphate and paraoxon.1 Publication
pH dependencei
Optimum pH is 8.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 994 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 1114 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 179 – 306 | cNMP 1Add BLAST | 128 | |
Nucleotide bindingi | 492 – 614 | cNMP 2Add BLAST | 123 | |
Nucleotide bindingi | 610 – 730 | cNMP 3Add BLAST | 121 |
GO - Molecular functioni
- carboxylic ester hydrolase activity Source: MGI
- lysophospholipase activity Source: UniProtKB
- phosphatidyl phospholipase B activity Source: UniProtKB-EC
GO - Biological processi
- angiogenesis Source: MGI
- animal organ morphogenesis Source: MGI
- lipid catabolic process Source: UniProtKB-KW
- phosphatidylcholine metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid degradation, Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-MMU-6814848, Glycerophospholipid catabolism |
Chemistry databases
SwissLipidsi | SLP:000001979 |
Names & Taxonomyi
Protein namesi | Recommended name: Patatin-like phospholipase domain-containing protein 6CuratedAlternative name(s): Neuropathy target esterase (EC:3.1.1.51 Publication1 Publication) |
Gene namesi | Name:Pnpla6 Synonyms:Nte |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1354723, Pnpla6 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass type III membrane protein 1 Publication
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 43 | LumenalSequence analysisAdd BLAST | 43 | |
Transmembranei | 44 – 64 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 65 – 1355 | CytoplasmicSequence analysisAdd BLAST | 1291 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Embryonically lethal. At 9 dpc, embryos are smaller, and their development is delayed (PubMed:12640454). At 10-11 dpc, the development is arrested with signs of resorption (PubMed:12640454). Heterozygous mice have lower catalytic activity towards the synthetic compound phenyl valerate in the brain and show increased motor activity (PubMed:12640454).1 Publication
Chemistry databases
ChEMBLi | CHEMBL3259506 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000292200 | 1 – 1355 | Patatin-like phospholipase domain-containing protein 6Add BLAST | 1355 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 9 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 338 | PhosphoserineBy similarity | 1 | |
Modified residuei | 345 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 346 | PhosphoserineBy similarity | 1 | |
Modified residuei | 356 | PhosphoserineBy similarity | 1 | |
Modified residuei | 405 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Glycosylated.By similarity
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q3TRM4 |
MaxQBi | Q3TRM4 |
PaxDbi | Q3TRM4 |
PeptideAtlasi | Q3TRM4 |
PRIDEi | Q3TRM4 |
ProteomicsDBi | 289688 [Q3TRM4-1] 289689 [Q3TRM4-2] 289690 [Q3TRM4-3] 289691 [Q3TRM4-4] |
PTM databases
GlyConnecti | 2555, 1 N-Linked glycan (1 site) |
GlyGeni | Q3TRM4, 1 site |
iPTMneti | Q3TRM4 |
PhosphoSitePlusi | Q3TRM4 |
SwissPalmi | Q3TRM4 |
Expressioni
Tissue specificityi
Expressed in brain, testes and kidney (at protein level) (PubMed:12640454). Expressed ubiquitously in brain of young mice (PubMed:10640712). Reaching adulthood, there is a most prominent expression in Purkinje cells, granule cells and pyramidal neurons of the hippocampus and some large neurons in the medulla oblongata, nucleus dentatus and pons (PubMed:10640712).2 Publications
Developmental stagei
Expressed in the embryonic respiratory system, different epithelial structures and strongly in the spinal ganglia, during the development.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000004565, Expressed in cerebellum and 301 other tissues |
ExpressionAtlasi | Q3TRM4, baseline and differential |
Genevisiblei | Q3TRM4, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 206099, 5 interactors |
STRINGi | 10090.ENSMUSP00000106699 |
Miscellaneous databases
RNActi | Q3TRM4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 961 – 1127 | PNPLAPROSITE-ProRule annotationAdd BLAST | 167 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 965 – 970 | GXGXXGPROSITE-ProRule annotation | 6 | |
Motifi | 992 – 996 | GXSXGPROSITE-ProRule annotation | 5 | |
Motifi | 1114 – 1116 | DGA/GPROSITE-ProRule annotation | 3 |
Sequence similaritiesi
Belongs to the NTE family.Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2968, Eukaryota |
GeneTreei | ENSGT00940000159130 |
HOGENOMi | CLU_000960_1_0_1 |
InParanoidi | Q3TRM4 |
OrthoDBi | 253518at2759 |
PhylomeDBi | Q3TRM4 |
TreeFami | TF300519 |
Family and domain databases
CDDi | cd00038, CAP_ED, 3 hits |
Gene3Di | 2.60.120.10, 3 hits |
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR018490, cNMP-bd-like IPR000595, cNMP-bd_dom IPR001423, LysoPLipase_patatin_CS IPR002641, PNPLA_dom IPR014710, RmlC-like_jellyroll |
Pfami | View protein in Pfam PF00027, cNMP_binding, 3 hits PF01734, Patatin, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 3 hits |
SUPFAMi | SSF51206, SSF51206, 3 hits SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50042, CNMP_BINDING_3, 3 hits PS51635, PNPLA, 1 hit PS01237, UPF0028, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q3TRM4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGTPSHELNT TSSGAEVIQK TLEEGLGRRI CVAQPVPFVP QVLGVMIGAG
60 70 80 90 100
VAVLVTAVLI LLVVRRLRVQ KTPAPEGPRY RFRKRDKVLF YGRKIMRKVS
110 120 130 140 150
QSTSSLVDTS VSTTSRPRMK KKLKMLNIAK KILRIQKETP TLQRKEPPPS
160 170 180 190 200
VLEADLTEGD LANSHLPSEV LYMLKNVRVL GHFEKPLFLE LCRHMVFQRL
210 220 230 240 250
GQGDYVFRPG QPDASIYVVQ DGLLELCLPG PDGKECVVKE VVPGDSVNSL
260 270 280 290 300
LSILDVITGH QHPQRTVSAR AARDSTVLRL PVEAFSAVFT KYPESLVRVV
310 320 330 340 350
QIIMVRLQRV TFLALHNYLG LTNELFSHEI QPLRLFPSPG LPTRTSPVRG
360 370 380 390 400
SKRVVSTSGT EDTSKETSGR PLDSIGAPLP GPAGDPVKPT SLEAPPAPLL
410 420 430 440 450
SRCISMPVDI SGLQGGPRSD FDMAYERGRI SVSLQEEASG GPQTASPREL
460 470 480 490 500
REQPAGACEY SYCEDESATG GCPFGPYQGR QTSSIFEAAK RELAKLMRIE
510 520 530 540 550
DPSLLNSRVL LHHAKAGTII ARQGDQDVSL HFVLWGCLHV YQRMIDKAEE
560 570 580 590 600
VCLFVAQPGE LVGQLAVLTG EPLIFTLRAQ RDCTFLRISK SHFYEIMRAQ
610 620 630 640 650
PSVVLSAAHT VAARMSPFVR QMDFAIDWTA VEAGRALYRQ GDRSDCTYIV
660 670 680 690 700
LNGRLRSVIQ RGSGKKELVG EYGRGDLIGV VEALTRQPRA TTVHAVRDTE
710 720 730 740 750
LAKLPEGTLG HIKRRYPQVV TRLIHLLSQK ILGNLQQLQG PFPGSGLSVP
760 770 780 790 800
QHSELTNPAS NLSTVAILPV CAEVPMMAFT LELQHALQAI GPTLLLNSDV
810 820 830 840 850
IRALLGASAL DSIQEFRLSG WLAQQEDAHR IVLYQTDTSL TPWTVRCLRQ
860 870 880 890 900
ADCILIVGLG DQEPTVGQLE QMLENTAVRA LKQLVLLHRE EGPGPTRTVE
910 920 930 940 950
WLNMRSWCSG HLHLRCPRRL FSRRSPAKLH ELYEKVFSRR ADRHSDFSRL
960 970 980 990 1000
ARVLTGNTIA LVLGGGGARG CSHIGVLKAL EEAGVPVDLV GGTSIGSFIG
1010 1020 1030 1040 1050
ALYAEERSAS RTKQRAREWA KSMTSVLEPV LDLTYPVTSM FTGSAFNRSI
1060 1070 1080 1090 1100
HRVFQDKQIE DLWLPYFNVT TDITASAMRV HKDGSLWRYV RASMTLSGYL
1110 1120 1130 1140 1150
PPLCDPKDGH LLMDGGYINN LPADIARSMG AKTVIAIDVG SQDETDLSTY
1160 1170 1180 1190 1200
GDSLSGWWLL WKRLNPWADK VKVPDMAEIQ SRLAYVSCVR QLEVVKSSSY
1210 1220 1230 1240 1250
CEYLRPSIDC FKTMDFGKFD QIYDVGYQYG KAVFGGWTRG EVIEKMLTDR
1260 1270 1280 1290 1300
RSTDLNESRR ADILAFPSSG FTDLAEIVSR IEPPTSYVSD GCADGEESDC
1310 1320 1330 1340 1350
LTEYEEDAGP DCSRDEGGSP EGASPSTASE VEEEKSTLRQ RRFLPQETPS
SVADA
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140LI54 | A0A140LI54_MOUSE | Patatin-like phospholipase domain-c... | Pnpla6 | 1,345 | Annotation score: | ||
A0A140LHU9 | A0A140LHU9_MOUSE | Patatin-like phospholipase domain-c... | Pnpla6 | 1,316 | Annotation score: | ||
A0A140LHE0 | A0A140LHE0_MOUSE | Patatin-like phospholipase domain-c... | Pnpla6 | 224 | Annotation score: | ||
A0A140LJH3 | A0A140LJH3_MOUSE | Patatin-like phospholipase domain-c... | Pnpla6 | 150 | Annotation score: | ||
A0A140LHJ7 | A0A140LHJ7_MOUSE | Patatin-like phospholipase domain-c... | Pnpla6 | 109 | Annotation score: | ||
A0A140LJI6 | A0A140LJI6_MOUSE | Patatin-like phospholipase domain-c... | Pnpla6 | 201 | Annotation score: | ||
A0A140LHT9 | A0A140LHT9_MOUSE | Patatin-like phospholipase domain-c... | Pnpla6 | 207 | Annotation score: | ||
A0A140LIJ8 | A0A140LIJ8_MOUSE | Patatin-like phospholipase domain-c... | Pnpla6 | 49 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 240 | E → K in AAD51700 (PubMed:10640712).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_026390 | 1 – 41 | MGTPS…PFVPQ → MEAPLQTGM in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 41 | |
Alternative sequenceiVSP_026391 | 448 | R → RTPTQ in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_026392 | 1123 – 1169 | ADIAR…NPWAD → GKWLPTHICMDTYHQTHAHT DFCTCRLEGTGLYEWRSSRG HTQLCTEL in isoform 4. 1 PublicationAdd BLAST | 47 | |
Alternative sequenceiVSP_026393 | 1170 – 1355 | Missing in isoform 4. 1 PublicationAdd BLAST | 186 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF173829 mRNA Translation: AAD51700.1 AK162641 mRNA Translation: BAE37004.1 AC170806 Genomic DNA No translation available. BC054789 mRNA Translation: AAH54789.1 BC056999 mRNA Translation: AAH56999.1 |
CCDSi | CCDS40206.1 [Q3TRM4-3] |
RefSeqi | NP_001116290.2, NM_001122818.2 NP_056616.2, NM_015801.2 [Q3TRM4-3] XP_006508887.1, XM_006508824.3 XP_006508888.2, XM_006508825.3 XP_006508893.2, XM_006508830.3 XP_017168379.1, XM_017312890.1 |
Genome annotation databases
Ensembli | ENSMUST00000004681; ENSMUSP00000004681; ENSMUSG00000004565 [Q3TRM4-3] ENSMUST00000111070; ENSMUSP00000106699; ENSMUSG00000004565 [Q3TRM4-3] |
GeneIDi | 50767 |
KEGGi | mmu:50767 |
UCSCi | uc009krr.1, mouse [Q3TRM4-3] uc009krs.1, mouse [Q3TRM4-2] uc009krt.1, mouse [Q3TRM4-4] uc009kru.1, mouse [Q3TRM4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF173829 mRNA Translation: AAD51700.1 AK162641 mRNA Translation: BAE37004.1 AC170806 Genomic DNA No translation available. BC054789 mRNA Translation: AAH54789.1 BC056999 mRNA Translation: AAH56999.1 |
CCDSi | CCDS40206.1 [Q3TRM4-3] |
RefSeqi | NP_001116290.2, NM_001122818.2 NP_056616.2, NM_015801.2 [Q3TRM4-3] XP_006508887.1, XM_006508824.3 XP_006508888.2, XM_006508825.3 XP_006508893.2, XM_006508830.3 XP_017168379.1, XM_017312890.1 |
3D structure databases
SMRi | Q3TRM4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206099, 5 interactors |
STRINGi | 10090.ENSMUSP00000106699 |
Chemistry databases
ChEMBLi | CHEMBL3259506 |
SwissLipidsi | SLP:000001979 |
PTM databases
GlyConnecti | 2555, 1 N-Linked glycan (1 site) |
GlyGeni | Q3TRM4, 1 site |
iPTMneti | Q3TRM4 |
PhosphoSitePlusi | Q3TRM4 |
SwissPalmi | Q3TRM4 |
Proteomic databases
jPOSTi | Q3TRM4 |
MaxQBi | Q3TRM4 |
PaxDbi | Q3TRM4 |
PeptideAtlasi | Q3TRM4 |
PRIDEi | Q3TRM4 |
ProteomicsDBi | 289688 [Q3TRM4-1] 289689 [Q3TRM4-2] 289690 [Q3TRM4-3] 289691 [Q3TRM4-4] |
Protocols and materials databases
Antibodypediai | 2222, 239 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000004681; ENSMUSP00000004681; ENSMUSG00000004565 [Q3TRM4-3] ENSMUST00000111070; ENSMUSP00000106699; ENSMUSG00000004565 [Q3TRM4-3] |
GeneIDi | 50767 |
KEGGi | mmu:50767 |
UCSCi | uc009krr.1, mouse [Q3TRM4-3] uc009krs.1, mouse [Q3TRM4-2] uc009krt.1, mouse [Q3TRM4-4] uc009kru.1, mouse [Q3TRM4-1] |
Organism-specific databases
CTDi | 10908 |
MGIi | MGI:1354723, Pnpla6 |
Phylogenomic databases
eggNOGi | KOG2968, Eukaryota |
GeneTreei | ENSGT00940000159130 |
HOGENOMi | CLU_000960_1_0_1 |
InParanoidi | Q3TRM4 |
OrthoDBi | 253518at2759 |
PhylomeDBi | Q3TRM4 |
TreeFami | TF300519 |
Enzyme and pathway databases
Reactomei | R-MMU-6814848, Glycerophospholipid catabolism |
Miscellaneous databases
BioGRID-ORCSi | 50767, 5 hits in 52 CRISPR screens |
PROi | PR:Q3TRM4 |
RNActi | Q3TRM4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000004565, Expressed in cerebellum and 301 other tissues |
ExpressionAtlasi | Q3TRM4, baseline and differential |
Genevisiblei | Q3TRM4, MM |
Family and domain databases
CDDi | cd00038, CAP_ED, 3 hits |
Gene3Di | 2.60.120.10, 3 hits |
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR018490, cNMP-bd-like IPR000595, cNMP-bd_dom IPR001423, LysoPLipase_patatin_CS IPR002641, PNPLA_dom IPR014710, RmlC-like_jellyroll |
Pfami | View protein in Pfam PF00027, cNMP_binding, 3 hits PF01734, Patatin, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 3 hits |
SUPFAMi | SSF51206, SSF51206, 3 hits SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50042, CNMP_BINDING_3, 3 hits PS51635, PNPLA, 1 hit PS01237, UPF0028, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLPL6_MOUSE | |
Accessioni | Q3TRM4Primary (citable) accession number: Q3TRM4 Secondary accession number(s): Q7TQD6, Q9R114 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 26, 2007 |
Last sequence update: | June 26, 2007 | |
Last modified: | April 7, 2021 | |
This is version 125 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families