UniProtKB - Q3TIR3 (RIC8A_MOUSE)
Protein
Synembryn-A
Gene
Ric8a
Organism
Mus musculus (Mouse)
Status
Functioni
Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (By similarity).By similarity
GO - Molecular functioni
- G-protein alpha-subunit binding Source: MGI
- GTPase activator activity Source: MGI
- guanyl-nucleotide exchange factor activity Source: MGI
GO - Biological processi
- adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: MGI
- basement membrane organization Source: MGI
- cell-cell adhesion involved in gastrulation Source: MGI
- cell migration involved in gastrulation Source: MGI
- gastrulation Source: MGI
- G protein-coupled receptor signaling pathway Source: GO_Central
- in utero embryonic development Source: MGI
- regulation of G protein-coupled receptor signaling pathway Source: MGI
- response to light stimulus Source: MGI
- vasculature development Source: MGI
- visual learning Source: MGI
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Names & Taxonomyi
Protein namesi | Recommended name: Synembryn-AAlternative name(s): Protein Ric-8A |
Gene namesi | Name:Ric8a Synonyms:Ric8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2141866, Ric8a |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Other locations
- Cytoplasm By similarity
Note: Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity). Colocalizes with RIC8A in CA2 hippocampal neurons.By similarity1 Publication
Cytosol
- cytosol Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Disruption phenotypei
Death during early embryonic development. Heterozygous mice exhibit impaired spatial memory and decreased anxiety.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000235892 | 1 – 530 | Synembryn-AAdd BLAST | 530 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 435 | PhosphoserineCombined sources | 1 | |
Modified residuei | 440 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 442 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 501 | PhosphoserineBy similarity | 1 | |
Modified residuei | 522 | PhosphoserineBy similarity | 1 | |
Modified residuei | 523 | PhosphoserineBy similarity | 1 | |
Modified residuei | 527 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q3TIR3 |
jPOSTi | Q3TIR3 |
MaxQBi | Q3TIR3 |
PaxDbi | Q3TIR3 |
PeptideAtlasi | Q3TIR3 |
PRIDEi | Q3TIR3 |
PTM databases
iPTMneti | Q3TIR3 |
PhosphoSitePlusi | Q3TIR3 |
SwissPalmi | Q3TIR3 |
Expressioni
Tissue specificityi
Expressed in neurons and neurites of the CA1 and CA2 subregions of the hippocampus (at protein level). In adult brain, it is expressed in the neocortex, hippocampus and cerebellum as well as in the pineal gland and ependymal layer.2 Publications
Developmental stagei
During the early development (9.5-12.0 dpc) it is expressed in the developing nervous system such as the cranial ganglia, neural tube, sympathetic chain and dorsal root ganglia. Also found in the lens, vomeronasal organ and endolymphatic sac.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000025485, Expressed in supraoptic nucleus and 301 other tissues |
Genevisiblei | Q3TIR3, MM |
Interactioni
Subunit structurei
Interacts with GDP-bound G alpha proteins GNAI1, GNAO1 and GNAQ, and with GNA13 with lower affinity. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma.
Interacts (via C-terminus) with RGS14; the interaction stimulates the dissociation of the complex between RGS14 and the active GTP-bound form of GNAI1 (By similarity).
By similarityGO - Molecular functioni
- G-protein alpha-subunit binding Source: MGI
- guanyl-nucleotide exchange factor activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 221670, 8 interactors |
IntActi | Q3TIR3, 1 interactor |
STRINGi | 10090.ENSMUSP00000026558 |
Miscellaneous databases
RNActi | Q3TIR3, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the synembryn family.Curated
Phylogenomic databases
eggNOGi | KOG4464, Eukaryota |
GeneTreei | ENSGT00390000014700 |
HOGENOMi | CLU_018602_1_0_1 |
InParanoidi | Q3TIR3 |
OMAi | NQEHSQS |
OrthoDBi | 1110075at2759 |
PhylomeDBi | Q3TIR3 |
TreeFami | TF314907 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR019318, Gua_nucleotide_exch_fac_Ric8 IPR008376, Synembryn |
PANTHERi | PTHR12425, PTHR12425, 1 hit |
Pfami | View protein in Pfam PF10165, Ric8, 1 hit |
PRINTSi | PR01802, SYNEMBRYN |
SUPFAMi | SSF48371, SSF48371, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q3TIR3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPRAVADAL ETGEEDAVTE ALRSFNREHS QSFTFDDAQQ EDRKRLAKLL
60 70 80 90 100
VSVLEQGLSP KHRVTWLQTI RILSRDRSCL DSFASRQSLH ALACYADITV
110 120 130 140 150
SEEPIPQSPD MDVLLESLKC LCNLVLSSPT AQMLAAEARL VVRLAERVGL
160 170 180 190 200
YRKRSYPHEV QFFDLRLLFL LTALRTDVRQ QLFQELHGVR LLTDALELTL
210 220 230 240 250
GVAPKENPPV MLPAQETERA MEILKVLFNI TFDSVKREVD EEDAALYRYL
260 270 280 290 300
GTLLRHCVMV EAAGDRTEEF HGHTVNLLGN LPLKCLDVLL ALELHEGSLE
310 320 330 340 350
FMGVNMDVIS ALLAFLEKRL HQTHRLKECV APVLNVLTEC ARMHRPARKF
360 370 380 390 400
LKAQVLPPLR DVRTRPEVGD LLRNKLVRLM THLDTDVKRV AAEFLFVLCS
410 420 430 440 450
ESVPRFIKYT GYGNAAGLLA ARGLMAGGRP EGQYSEDEDT DTEEYREAKA
460 470 480 490 500
SINPVTGRVE EKPPNPMEGM TEEQKEHEAM KLVNMFDKLS RHRVIQPMGM
510 520 530
SPRGHLTSLQ DAMCETMEGQ LSSDPDSDPD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GSI8 | A0A1B0GSI8_MOUSE | Synembryn-A | Ric8a | 328 | Annotation score: | ||
A0A1B0GS30 | A0A1B0GS30_MOUSE | Synembryn-A | Ric8a | 160 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 87 | Q → R in BAE41115 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 274 | T → A in BAE39783 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 449 | K → E in BAE39783 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF288813 mRNA Translation: AAG10200.1 AK031466 mRNA Translation: BAC27418.1 AK080192 mRNA Translation: BAC37844.1 AK155282 mRNA Translation: BAE33163.1 AK167746 mRNA Translation: BAE39783.1 AK169367 mRNA Translation: BAE41115.1 BC022917 mRNA Translation: AAH22917.1 |
CCDSi | CCDS21988.1 |
RefSeqi | NP_444424.1, NM_053194.4 |
Genome annotation databases
Ensembli | ENSMUST00000026558; ENSMUSP00000026558; ENSMUSG00000025485 |
GeneIDi | 101489 |
KEGGi | mmu:101489 |
UCSCi | uc009kij.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF288813 mRNA Translation: AAG10200.1 AK031466 mRNA Translation: BAC27418.1 AK080192 mRNA Translation: BAC37844.1 AK155282 mRNA Translation: BAE33163.1 AK167746 mRNA Translation: BAE39783.1 AK169367 mRNA Translation: BAE41115.1 BC022917 mRNA Translation: AAH22917.1 |
CCDSi | CCDS21988.1 |
RefSeqi | NP_444424.1, NM_053194.4 |
3D structure databases
SMRi | Q3TIR3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 221670, 8 interactors |
IntActi | Q3TIR3, 1 interactor |
STRINGi | 10090.ENSMUSP00000026558 |
PTM databases
iPTMneti | Q3TIR3 |
PhosphoSitePlusi | Q3TIR3 |
SwissPalmi | Q3TIR3 |
Proteomic databases
EPDi | Q3TIR3 |
jPOSTi | Q3TIR3 |
MaxQBi | Q3TIR3 |
PaxDbi | Q3TIR3 |
PeptideAtlasi | Q3TIR3 |
PRIDEi | Q3TIR3 |
Protocols and materials databases
ABCDi | Q3TIR3, 3 sequenced antibodies |
Antibodypediai | 22381, 264 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000026558; ENSMUSP00000026558; ENSMUSG00000025485 |
GeneIDi | 101489 |
KEGGi | mmu:101489 |
UCSCi | uc009kij.1, mouse |
Organism-specific databases
CTDi | 60626 |
MGIi | MGI:2141866, Ric8a |
Phylogenomic databases
eggNOGi | KOG4464, Eukaryota |
GeneTreei | ENSGT00390000014700 |
HOGENOMi | CLU_018602_1_0_1 |
InParanoidi | Q3TIR3 |
OMAi | NQEHSQS |
OrthoDBi | 1110075at2759 |
PhylomeDBi | Q3TIR3 |
TreeFami | TF314907 |
Miscellaneous databases
BioGRID-ORCSi | 101489, 5 hits in 19 CRISPR screens |
PROi | PR:Q3TIR3 |
RNActi | Q3TIR3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025485, Expressed in supraoptic nucleus and 301 other tissues |
Genevisiblei | Q3TIR3, MM |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR019318, Gua_nucleotide_exch_fac_Ric8 IPR008376, Synembryn |
PANTHERi | PTHR12425, PTHR12425, 1 hit |
Pfami | View protein in Pfam PF10165, Ric8, 1 hit |
PRINTSi | PR01802, SYNEMBRYN |
SUPFAMi | SSF48371, SSF48371, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIC8A_MOUSE | |
Accessioni | Q3TIR3Primary (citable) accession number: Q3TIR3 Secondary accession number(s): Q3TEY3, Q99JW0, Q9ERR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | May 16, 2006 | |
Last modified: | August 12, 2020 | |
This is version 117 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families