UniProtKB - Q3THK3 (T2FA_MOUSE)
Protein
General transcription factor IIF subunit 1
Gene
Gtf2f1
Organism
Mus musculus (Mouse)
Status
Functioni
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation (By similarity).By similarity
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- phosphatase activator activity Source: MGI
- promoter-specific chromatin binding Source: UniProtKB
- protein domain specific binding Source: MGI
- protein phosphatase binding Source: MGI
- RNA polymerase II general transcription initiation factor activity Source: MGI
- TFIIF-class transcription factor complex binding Source: GO_Central
- transcription factor binding Source: MGI
GO - Biological processi
- negative regulation of protein binding Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of transcription elongation from RNA polymerase II promoter Source: InterPro
- response to virus Source: Ensembl
- transcription elongation from RNA polymerase II promoter Source: GO_Central
- transcription initiation from RNA polymerase II promoter Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-113418, Formation of the Early Elongation Complex R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-6803529, FGFR2 alternative splicing R-MMU-6807505, RNA polymerase II transcribes snRNA genes R-MMU-72086, mRNA Capping R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72165, mRNA Splicing - Minor Pathway R-MMU-72203, Processing of Capped Intron-Containing Pre-mRNA R-MMU-73776, RNA Polymerase II Promoter Escape R-MMU-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-MMU-75953, RNA Polymerase II Transcription Initiation R-MMU-75955, RNA Polymerase II Transcription Elongation R-MMU-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-MMU-77075, RNA Pol II CTD phosphorylation and interaction with CE R-MMU-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: General transcription factor IIF subunit 1Alternative name(s): Transcription initiation factor IIF subunit alpha Short name: TFIIF-alpha |
Gene namesi | Name:Gtf2f1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1923848, Gtf2f1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
- transcription factor TFIID complex Source: MGI
- transcription factor TFIIF complex Source: GO_Central
Other locations
- cell junction Source: MGI
- intracellular membrane-bounded organelle Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000260322 | 2 – 508 | General transcription factor IIF subunit 1Add BLAST | 507 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 156 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 217 | PhosphoserineCombined sources | 1 | |
Modified residuei | 218 | PhosphoserineCombined sources | 1 | |
Modified residuei | 221 | PhosphoserineCombined sources | 1 | |
Modified residuei | 224 | PhosphoserineCombined sources | 1 | |
Modified residuei | 331 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 377 | PhosphoserineBy similarity | 1 | |
Modified residuei | 380 | PhosphoserineBy similarity | 1 | |
Modified residuei | 381 | PhosphoserineBy similarity | 1 | |
Modified residuei | 385 | PhosphoserineBy similarity | 1 | |
Modified residuei | 389 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 391 | PhosphoserineBy similarity | 1 | |
Modified residuei | 407 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 431 | PhosphoserineBy similarity | 1 | |
Modified residuei | 433 | PhosphoserineCombined sources | 1 | |
Modified residuei | 436 | PhosphoserineBy similarity | 1 | |
Modified residuei | 437 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 440 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q3THK3 |
jPOSTi | Q3THK3 |
MaxQBi | Q3THK3 |
PaxDbi | Q3THK3 |
PeptideAtlasi | Q3THK3 |
PRIDEi | Q3THK3 |
PTM databases
iPTMneti | Q3THK3 |
PhosphoSitePlusi | Q3THK3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000002658, Expressed in vestibular organ and 322 other tissues |
Genevisiblei | Q3THK3, MM |
Interactioni
Subunit structurei
Heterodimer of an alpha and a beta subunit.
Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1 (By similarity).
Interacts with GTF2B (via C-terminus and preferentially via acetylated form); this interaction prevents binding of GTF2B to GTF2F2 (By similarity).
By similarityGO - Molecular functioni
- protein domain specific binding Source: MGI
- protein phosphatase binding Source: MGI
- TFIIF-class transcription factor complex binding Source: GO_Central
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 220965, 30 interactors |
ComplexPortali | CPX-83, Transcription factor TFIIF complex |
CORUMi | Q3THK3 |
IntActi | Q3THK3, 1 interactor |
MINTi | Q3THK3 |
STRINGi | 10090.ENSMUSP00000002733 |
Miscellaneous databases
RNActi | Q3THK3, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 283 – 350 | Glu-richAdd BLAST | 68 |
Sequence similaritiesi
Belongs to the TFIIF alpha subunit family.Curated
Phylogenomic databases
eggNOGi | KOG2393, Eukaryota |
GeneTreei | ENSGT00440000038032 |
HOGENOMi | CLU_027572_2_0_1 |
InParanoidi | Q3THK3 |
OMAi | AFYVFTH |
OrthoDBi | 893557at2759 |
TreeFami | TF313850 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR008851, TFIIF-alpha IPR011039, TFIIF_interaction IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR13011, PTHR13011, 1 hit |
Pfami | View protein in Pfam PF05793, TFIIF_alpha, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF50916, SSF50916, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q3THK3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAALGSSSQN VTEYVVRVPK NTAKRYNIMA FNAADKVNFA TWNQARLERD
60 70 80 90 100
LSNKKIYQEE EMPESGAGSE FNRKLREEAR RKKYGIVLKE FRPEDQPWLL
110 120 130 140 150
RVNGKSGRKF KGIKKGGVTE NTAYYIFTQC ADGAFEAFPV QNWYNFTPLA
160 170 180 190 200
RHRTLTAEEA EEEWERRNKV LNHFSIMQQR RLKDQDQDED EEEKEKRSRK
210 220 230 240 250
KPSELRIHDL EDDLEMSSDA SDASGEEGSR TSKAKKKAPV TKAGRKKKKK
260 270 280 290 300
KGSDDEAFED SDDGDFEGQE VDYMSDGSSS SPDETEGKPK VPQQEDGPKG
310 320 330 340 350
VDEQSESSEE SEEEKPPEED KEEEEEKKAP TPQEKKRRKD SSDDSDSSEE
360 370 380 390 400
SDIDSETSSA LFMAKKKTPP KRERKPSGGS SKGTSRPGTP SAEAASTSST
410 420 430 440 450
LRAAASKLEQ GKRTSETPAA KRLRMDTGPQ SLSGKSTPSS GDVQVTEDAV
460 470 480 490 500
RRYLTRKPMT TKDLLKKFQT KKTGLSSEQT VNVLAQILKR LNPERKMIGD
KMHFSLKE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 139 | P → T in BAE40186 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 139 | P → T in BAE40193 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 185 | Q → E in BAE40186 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 185 | Q → E in BAE40193 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK168236 mRNA Translation: BAE40186.1 AK168243 mRNA Translation: BAE40193.1 CH466537 Genomic DNA Translation: EDL38221.1 BC023081 mRNA Translation: AAH23081.1 BC031123 mRNA Translation: AAH31123.1 |
CCDSi | CCDS37667.1 |
RefSeqi | NP_598562.1, NM_133801.2 |
Genome annotation databases
Ensembli | ENSMUST00000002733; ENSMUSP00000002733; ENSMUSG00000002658 |
GeneIDi | 98053 |
KEGGi | mmu:98053 |
UCSCi | uc008ddp.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK168236 mRNA Translation: BAE40186.1 AK168243 mRNA Translation: BAE40193.1 CH466537 Genomic DNA Translation: EDL38221.1 BC023081 mRNA Translation: AAH23081.1 BC031123 mRNA Translation: AAH31123.1 |
CCDSi | CCDS37667.1 |
RefSeqi | NP_598562.1, NM_133801.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 220965, 30 interactors |
ComplexPortali | CPX-83, Transcription factor TFIIF complex |
CORUMi | Q3THK3 |
IntActi | Q3THK3, 1 interactor |
MINTi | Q3THK3 |
STRINGi | 10090.ENSMUSP00000002733 |
PTM databases
iPTMneti | Q3THK3 |
PhosphoSitePlusi | Q3THK3 |
Proteomic databases
EPDi | Q3THK3 |
jPOSTi | Q3THK3 |
MaxQBi | Q3THK3 |
PaxDbi | Q3THK3 |
PeptideAtlasi | Q3THK3 |
PRIDEi | Q3THK3 |
Protocols and materials databases
Antibodypediai | 11855, 276 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000002733; ENSMUSP00000002733; ENSMUSG00000002658 |
GeneIDi | 98053 |
KEGGi | mmu:98053 |
UCSCi | uc008ddp.1, mouse |
Organism-specific databases
CTDi | 2962 |
MGIi | MGI:1923848, Gtf2f1 |
Phylogenomic databases
eggNOGi | KOG2393, Eukaryota |
GeneTreei | ENSGT00440000038032 |
HOGENOMi | CLU_027572_2_0_1 |
InParanoidi | Q3THK3 |
OMAi | AFYVFTH |
OrthoDBi | 893557at2759 |
TreeFami | TF313850 |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-113418, Formation of the Early Elongation Complex R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-6803529, FGFR2 alternative splicing R-MMU-6807505, RNA polymerase II transcribes snRNA genes R-MMU-72086, mRNA Capping R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72165, mRNA Splicing - Minor Pathway R-MMU-72203, Processing of Capped Intron-Containing Pre-mRNA R-MMU-73776, RNA Polymerase II Promoter Escape R-MMU-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-MMU-75953, RNA Polymerase II Transcription Initiation R-MMU-75955, RNA Polymerase II Transcription Elongation R-MMU-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-MMU-77075, RNA Pol II CTD phosphorylation and interaction with CE R-MMU-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
BioGRID-ORCSi | 98053, 8 hits in 18 CRISPR screens |
PROi | PR:Q3THK3 |
RNActi | Q3THK3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000002658, Expressed in vestibular organ and 322 other tissues |
Genevisiblei | Q3THK3, MM |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR008851, TFIIF-alpha IPR011039, TFIIF_interaction IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR13011, PTHR13011, 1 hit |
Pfami | View protein in Pfam PF05793, TFIIF_alpha, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF50916, SSF50916, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | T2FA_MOUSE | |
Accessioni | Q3THK3Primary (citable) accession number: Q3THK3 Secondary accession number(s): Q8R5B7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families