Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myosin regulatory light chain 12B

Gene

Myl12b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.By similarity

Miscellaneous

This chain binds calcium.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi42 – 53PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • myosin heavy chain binding Source: MGI

GO - Biological processi

  • regulation of cell shape Source: MGI

Keywordsi

Molecular functionMotor protein, Muscle protein, Myosin
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-445355 Smooth Muscle Contraction
R-MMU-5627123 RHO GTPases activate PAKs

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 12B
Alternative name(s):
Myosin regulatory light chain 2-B, smooth muscle isoform
Myosin regulatory light chain 20 kDa
Short name:
MLC20
Myosin regulatory light chain MRLC2
Gene namesi
Name:Myl12b
Synonyms:Mrlc2, Mylc2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107494 Myl12b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002843831 – 172Myosin regulatory light chain 12BAdd BLAST172

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Phosphothreonine; by MLCK and ZIPK/DAPK3By similarity1
Modified residuei20Phosphoserine; by MLCK and ZIPK/DAPK3By similarity1

Post-translational modificationi

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3THE2
MaxQBiQ3THE2
PaxDbiQ3THE2
PeptideAtlasiQ3THE2
PRIDEiQ3THE2
TopDownProteomicsiQ3THE2

PTM databases

iPTMnetiQ3THE2
PhosphoSitePlusiQ3THE2
SwissPalmiQ3THE2

Expressioni

Gene expression databases

BgeeiENSMUSG00000034868 Expressed in 291 organ(s), highest expression level in CA1 field of hippocampus
CleanExiMM_MYLC2B
GenevisibleiQ3THE2 MM

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212551, 6 interactors
DIPiDIP-31955N
IntActiQ3THE2, 21 interactors
MINTiQ3THE2
STRINGi10090.ENSMUSP00000042364

Structurei

3D structure databases

ProteinModelPortaliQ3THE2
SMRiQ3THE2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 64EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini98 – 133EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini134 – 169EF-hand 3PROSITE-ProRule annotationAdd BLAST36

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0031 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000119196
HOGENOMiHOG000233018
InParanoidiQ3THE2
KOiK12757
OMAiDEEGTGM
OrthoDBiEOG091G0QF6
PhylomeDBiQ3THE2
TreeFamiTF314218

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR015070 EF_hand_DJBP
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF08976 EF-hand_11, 1 hit
PF13405 EF-hand_6, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 3 hits

Sequencei

Sequence statusi: Complete.

Q3THE2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSKKAKTKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID
60 70 80 90 100
KEDLHDMLAS LGKNPTDAYL DAMMNEAPGP INFTMFLTMF GEKLNGTDPE
110 120 130 140 150
DVIRNAFACF DEEATGTIQE DYLRELLTTM GDRFTDEEVD ELYREAPIDK
160 170
KGNFNYIEFT RILKHGAKDK DD
Length:172
Mass (Da):19,779
Last modified:April 17, 2007 - v2
Checksum:i78FF911630F3870B
GO

Sequence cautioni

The sequence BAE35646 differs from that shown. Reason: Frameshift at position 159.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45R → Q in BAE40255 (PubMed:16141072).Curated1
Sequence conflicti79G → A in BAE35646 (PubMed:16141072).Curated1
Sequence conflicti159F → V in BAE35646 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002885 mRNA Translation: BAB22432.1
AK005133 mRNA Translation: BAB23832.1
AK146115 mRNA Translation: BAE26912.1
AK160123 mRNA Translation: BAE35646.1 Frameshift.
AK168316 mRNA Translation: BAE40255.1
BC028878 mRNA Translation: AAH28878.1
BC099425 mRNA Translation: AAH99425.1
BC131925 mRNA Translation: AAI31926.1
BC131927 mRNA Translation: AAI31928.1
CCDSiCCDS28954.1
RefSeqiNP_075891.1, NM_023402.2
UniGeneiMm.261329

Genome annotation databases

EnsembliENSMUST00000038446; ENSMUSP00000042364; ENSMUSG00000034868
GeneIDi67938
KEGGimmu:67938
UCSCiuc008dlv.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002885 mRNA Translation: BAB22432.1
AK005133 mRNA Translation: BAB23832.1
AK146115 mRNA Translation: BAE26912.1
AK160123 mRNA Translation: BAE35646.1 Frameshift.
AK168316 mRNA Translation: BAE40255.1
BC028878 mRNA Translation: AAH28878.1
BC099425 mRNA Translation: AAH99425.1
BC131925 mRNA Translation: AAI31926.1
BC131927 mRNA Translation: AAI31928.1
CCDSiCCDS28954.1
RefSeqiNP_075891.1, NM_023402.2
UniGeneiMm.261329

3D structure databases

ProteinModelPortaliQ3THE2
SMRiQ3THE2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212551, 6 interactors
DIPiDIP-31955N
IntActiQ3THE2, 21 interactors
MINTiQ3THE2
STRINGi10090.ENSMUSP00000042364

PTM databases

iPTMnetiQ3THE2
PhosphoSitePlusiQ3THE2
SwissPalmiQ3THE2

Proteomic databases

EPDiQ3THE2
MaxQBiQ3THE2
PaxDbiQ3THE2
PeptideAtlasiQ3THE2
PRIDEiQ3THE2
TopDownProteomicsiQ3THE2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038446; ENSMUSP00000042364; ENSMUSG00000034868
GeneIDi67938
KEGGimmu:67938
UCSCiuc008dlv.1 mouse

Organism-specific databases

CTDi103910
MGIiMGI:107494 Myl12b

Phylogenomic databases

eggNOGiKOG0031 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000119196
HOGENOMiHOG000233018
InParanoidiQ3THE2
KOiK12757
OMAiDEEGTGM
OrthoDBiEOG091G0QF6
PhylomeDBiQ3THE2
TreeFamiTF314218

Enzyme and pathway databases

ReactomeiR-MMU-445355 Smooth Muscle Contraction
R-MMU-5627123 RHO GTPases activate PAKs

Miscellaneous databases

PROiPR:Q3THE2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034868 Expressed in 291 organ(s), highest expression level in CA1 field of hippocampus
CleanExiMM_MYLC2B
GenevisibleiQ3THE2 MM

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR015070 EF_hand_DJBP
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF08976 EF-hand_11, 1 hit
PF13405 EF-hand_6, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiML12B_MOUSE
AccessioniPrimary (citable) accession number: Q3THE2
Secondary accession number(s): Q3TVH2, Q9CQL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: April 17, 2007
Last modified: September 12, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again