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UniProtKB - Q3T1L5 (ALG6_RAT)
Protein
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
Gene
Alg6
Organism
Rattus norvegicus (Rat)
Status
Functioni
Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc2-PP-Dol (By similarity).
By similarityCatalytic activityi
- α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl β-D-glucosyl phosphate = α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl phosphate + H+EC:2.4.1.267
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: GO_Central
- glucosyltransferase activity Source: RGD
GO - Biological processi
- dolichol-linked oligosaccharide biosynthetic process Source: InterPro
- oligosaccharide-lipid intermediate biosynthetic process Source: GO_Central
- protein N-linked glycosylation Source: RGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-RNO-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT57, Glycosyltransferase Family 57 |
Names & Taxonomyi
Protein namesi | Recommended name: Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.267)Alternative name(s): Asparagine-linked glycosylation protein 6 homolog Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase |
Gene namesi | Name:Alg6 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1308815, Alg6 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Multi-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 4 – 24 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 115 – 135 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 144 – 164 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 173 – 193 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 227 – 247 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 298 – 318 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 324 – 344 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 362 – 382 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 388 – 408 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 438 – 458 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 473 – 493 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000284133 | 1 – 507 | Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST | 507 |
PTM databases
PhosphoSitePlusi | Q3T1L5 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ALG6/ALG8 glucosyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2575, Eukaryota |
InParanoidi | Q3T1L5 |
OrthoDBi | 595382at2759 |
PhylomeDBi | Q3T1L5 |
Family and domain databases
InterProi | View protein in InterPro IPR039488, ALG6 IPR004856, Glyco_trans_ALG6/ALG8 |
PANTHERi | PTHR12413, PTHR12413, 1 hit PTHR12413:SF1, PTHR12413:SF1, 1 hit |
Pfami | View protein in Pfam PF03155, Alg6_Alg8, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q3T1L5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESWTWMTVV VLLGLTVRWT VSLNSYSGAG KPPMFGDYEA QRHWQEITLN
60 70 80 90 100
LPVKQWYFNS SDNNLQYWGL DYPPLTAYHS LLCAYVAKFI NPDWVALHTS
110 120 130 140 150
RGYESQAHKL FMRTTVLAAD LLIYIPAVLL YCYSLKEISP KRKIASALCI
160 170 180 190 200
LLYPGLILID YGHFQYNSVS LGFALWGVLG VSCDWDLLGS LAFCLALNYK
210 220 230 240 250
QMELYHSLPF FCFLLGKCFK KGLRGKGSAL FIRIACTVVA SFLLCWLPFL
260 270 280 290 300
TEREHALQVV RRLFPVDRGL FEDKVANIWC SLNVFLKIKD ILPRHIQIAI
310 320 330 340 350
SFCFTFLSLL PACIKLTVQP SAKGFRFTLV SCALSFFLFS FQVHEKSILL
360 370 380 390 400
VSLPVCLVLT EIPFMSTWFL LVSTFSMLPL LLKDQLLLPS VVTVMAFLIA
410 420 430 440 450
CSTFFPMFEN TSEEQLQLKS FAVSVRRHLP GFTFLPRIIQ CLFLSSVITM
460 470 480 490 500
ILLTILSVTL DPPQKLPDLF SVLICFVSCV NFVFFLVYFN IVIMWDSKNG
RNRKKID
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LMD6 | F1LMD6_RAT | Alpha-1,3-glucosyltransferase | Alg6 | 507 | Annotation score: | ||
A0A8I6AJN8 | A0A8I6AJN8_RAT | Dolichyl pyrophosphate Man9GlcNAc2 ... | Alg6 | 487 | Annotation score: | ||
A0A8I6G6U1 | A0A8I6G6U1_RAT | Dolichyl pyrophosphate Man9GlcNAc2 ... | Alg6 | 486 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC101850 mRNA Translation: AAI01851.1 |
RefSeqi | NP_001028881.1, NM_001033709.1 |
Genome annotation databases
Ensembli | ENSRNOT00000012052; ENSRNOP00000012052; ENSRNOG00000009045 |
GeneIDi | 362547 |
KEGGi | rno:362547 |
UCSCi | RGD:1308815, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC101850 mRNA Translation: AAI01851.1 |
RefSeqi | NP_001028881.1, NM_001033709.1 |
3D structure databases
AlphaFoldDBi | Q3T1L5 |
SMRi | Q3T1L5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000012052 |
Protein family/group databases
CAZyi | GT57, Glycosyltransferase Family 57 |
PTM databases
PhosphoSitePlusi | Q3T1L5 |
Genome annotation databases
Ensembli | ENSRNOT00000012052; ENSRNOP00000012052; ENSRNOG00000009045 |
GeneIDi | 362547 |
KEGGi | rno:362547 |
UCSCi | RGD:1308815, rat |
Organism-specific databases
CTDi | 29929 |
RGDi | 1308815, Alg6 |
Phylogenomic databases
eggNOGi | KOG2575, Eukaryota |
InParanoidi | Q3T1L5 |
OrthoDBi | 595382at2759 |
PhylomeDBi | Q3T1L5 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-RNO-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
Miscellaneous databases
PROi | PR:Q3T1L5 |
Family and domain databases
InterProi | View protein in InterPro IPR039488, ALG6 IPR004856, Glyco_trans_ALG6/ALG8 |
PANTHERi | PTHR12413, PTHR12413, 1 hit PTHR12413:SF1, PTHR12413:SF1, 1 hit |
Pfami | View protein in Pfam PF03155, Alg6_Alg8, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALG6_RAT | |
Accessioni | Q3T1L5Primary (citable) accession number: Q3T1L5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 17, 2007 |
Last sequence update: | October 11, 2005 | |
Last modified: | May 25, 2022 | |
This is version 83 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families