UniProtKB - Q3T054 (RAN_BOVIN)
GTP-binding nuclear protein Ran
RAN
Functioni
GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Enhances AR-mediated transactivation.
By similarityCofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 68 | GTP; via amide nitrogenBy similarity | 1 | |
Sitei | 69 | Essential for GTP hydrolysisBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 18 – 25 | GTPBy similarity | 8 | |
Nucleotide bindingi | 36 – 42 | GTPBy similarity | 7 | |
Nucleotide bindingi | 122 – 125 | GTPBy similarity | 4 | |
Nucleotide bindingi | 150 – 152 | GTPBy similarity | 3 |
GO - Molecular functioni
- GDP binding Source: Ensembl
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- pre-miRNA binding Source: Ensembl
- protein heterodimerization activity Source: Ensembl
GO - Biological processi
- cell division Source: UniProtKB-KW
- GTP metabolic process Source: UniProtKB
- mitotic sister chromatid segregation Source: UniProtKB
- positive regulation of protein binding Source: Ensembl
- positive regulation of protein import into nucleus Source: Ensembl
- protein export from nucleus Source: UniProtKB
- protein import into nucleus Source: UniProtKB
- protein localization to nucleolus Source: Ensembl
- ribosomal large subunit export from nucleus Source: Ensembl
- ribosomal small subunit export from nucleus Source: Ensembl
- ribosomal subunit export from nucleus Source: GO_Central
- snRNA import into nucleus Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis, Protein transport, Transport |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: GTP-binding nuclear protein RanAlternative name(s): GTPase Ran Ras-related nuclear protein |
Gene namesi | Name:RAN |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000055033 |
Subcellular locationi
Cytoplasm and Cytosol
Nucleus
- Nucleus By similarity
- Nucleus envelope By similarity
Other locations
- Melanosome By similarity
Note: Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (By similarity).By similarity
Cytoskeleton
- centriole Source: Ensembl
Cytosol
- cytosol Source: UniProtKB-SubCell
Endosome
- recycling endosome Source: Ensembl
Nucleus
- nuclear envelope Source: UniProtKB-SubCell
- nucleolus Source: Ensembl
- nucleoplasm Source: Ensembl
- nucleus Source: UniProtKB
Other locations
- chromatin Source: Ensembl
- cytoplasm Source: GO_Central
- melanosome Source: UniProtKB-SubCell
- midbody Source: Ensembl
- RNA nuclear export complex Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000249772 | 2 – 216 | GTP-binding nuclear protein RanAdd BLAST | 215 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 24 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 37 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 60 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 71 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 71 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 71 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Modified residuei | 99 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 134 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 152 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 159 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 159 | N6-succinyllysine; alternateBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q3T054 |
PeptideAtlasi | Q3T054 |
PRIDEi | Q3T054 |
Interactioni
Subunit structurei
Monomer.
Interacts with RANGAP1, which promotes RAN-mediated GTP hydrolysis.
Interacts with KPNB1. Interaction with KPNB1 inhibits RANGAP1-mediated stimulation of GTPase activity.
Interacts with RCC1 which promotes the exchange of RAN-bound GDP by GTP. Interaction with KPNB1 inhibits RCC1-mediated exchange of RAN-bound GDP by GTP.
Interacts (GTP-bound form) with TNPO1; the interaction is direct.
Interacts (GTP-bound form) with TNPO3; the interaction is direct.
Interacts with KPNB1 and with TNPO1; both inhibit RAN GTPase activity.
Interacts (via C-terminus) with RANBP1, which alleviates the inhibition of RAN GTPase activity.
Interacts with RANGRF, which promotes the release of bound guanine nucleotide. RANGRF and RCC1 compete for an overlapping binding site on RAN.
Identified in a complex with KPNA2 and CSE1L; interaction with RANBP1 mediates dissociation of RAN from this complex. Interaction with both RANBP1 and KPNA2 promotes dissociation of the complex between RAN and KPNB1.
Identified in a complex composed of RAN, RANGAP1 and RANBP1.
Identified in a complex that contains TNPO1, RAN and RANBP1.
Identified in a nuclear export complex with XPO1.
Found in a nuclear export complex with RANBP3 and XPO1.
Interacts with RANBP2/NUP358. Interaction with RANBP1 or RANBP2 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins.
Component of a nuclear export receptor complex composed of KPNB1, RAN, SNUPN and XPO1 (By similarity).
Found in a nuclear export complex with RAN, XPO5 and pre-miRNA (By similarity).
Interacts (GTP-bound form) with XPO5 (By similarity).
Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5.
Interacts with RANBP9 and RANBP10.
Interacts in its GTP-bound form with BIRC5/survivin at S and M phases of the cell cycle.
Interacts with TERT; the interaction requires hydrogen peroxide-mediated phosphorylation of TERT and transports TERT to the nucleus.
Interacts with MAD2L2.
Interacts with VRK1 and VRK3.
Interacts with VRK2 (By similarity).
Interacts with NEMP1 and KPNB1 (By similarity).
Interacts (GDP-bound form) with NUTF2; regulates RAN nuclear import.
Interacts with CAPG; mediates CAPG nuclear import.
Interacts with NUP153.
Interacts with the AR N-terminal poly-Gln region; the interaction with AR is inversely correlated with the poly-Gln length (By similarity).
Interacts with MYCBP2, which promotes RAN-mediated GTP hydrolysis (By similarity).
Interacts with EPG5 (By similarity).
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: Ensembl
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 211 – 216 | Interaction with RANBP1By similarity | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0096, Eukaryota |
GeneTreei | ENSGT00940000153786 |
InParanoidi | Q3T054 |
OMAi | AQCGIIM |
OrthoDBi | 1083175at2759 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR002041, Ran_GTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
PANTHERi | PTHR24071, PTHR24071, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
PRINTSi | PR00627, GTPRANTC4 |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51418, RAN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL
60 70 80 90 100
VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN
110 120 130 140 150
VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS
160 170 180 190 200
AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD
210
LEVAQTTALP DEDDDL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC102559 mRNA Translation: AAI02560.1 |
RefSeqi | NP_001029877.1, NM_001034705.1 |
Genome annotation databases
Ensembli | ENSBTAT00000077257; ENSBTAP00000067034; ENSBTAG00000055033 |
GeneIDi | 540457 |
KEGGi | bta:540457 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC102559 mRNA Translation: AAI02560.1 |
RefSeqi | NP_001029877.1, NM_001034705.1 |
3D structure databases
BMRBi | Q3T054 |
SMRi | Q3T054 |
ModBasei | Search... |
Proteomic databases
PaxDbi | Q3T054 |
PeptideAtlasi | Q3T054 |
PRIDEi | Q3T054 |
Genome annotation databases
Ensembli | ENSBTAT00000077257; ENSBTAP00000067034; ENSBTAG00000055033 |
GeneIDi | 540457 |
KEGGi | bta:540457 |
Organism-specific databases
CTDi | 5901 |
VEuPathDBi | HostDB:ENSBTAG00000055033 |
Phylogenomic databases
eggNOGi | KOG0096, Eukaryota |
GeneTreei | ENSGT00940000153786 |
InParanoidi | Q3T054 |
OMAi | AQCGIIM |
OrthoDBi | 1083175at2759 |
Gene expression databases
ExpressionAtlasi | Q3T054, baseline and differential |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR002041, Ran_GTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
PANTHERi | PTHR24071, PTHR24071, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
PRINTSi | PR00627, GTPRANTC4 |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51418, RAN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAN_BOVIN | |
Accessioni | Q3T054Primary (citable) accession number: Q3T054 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2006 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 128 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families