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UniProtKB - Q3SYY2 (TPST2_BOVIN)
Protein
Protein-tyrosine sulfotransferase 2
Gene
TPST2
Organism
Bos taurus (Bovine)
Status
Functioni
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.
By similarityMiscellaneous
Substrate peptides must be flexible in order to adopt an L-shaped conformation in the deep binding cleft.By similarity
Catalytic activityi
- 3'-phosphoadenylyl sulfate + L-tyrosyl-[protein] = adenosine 3',5'-bisphosphate + H+ + O-sulfo-L-tyrosine-[protein]By similarityEC:2.8.2.20By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 99 | Proton donor/acceptorBy similarity | 1 | |
Sitei | 158 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 183 | PAPSBy similarity | 1 | |
Binding sitei | 191 | PAPSBy similarity | 1 | |
Binding sitei | 195 | PAPSBy similarity | 1 | |
Binding sitei | 238 | PAPSBy similarity | 1 | |
Sitei | 285 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 300 | PAPSBy similarity | 1 |
GO - Molecular functioni
- protein-tyrosine sulfotransferase activity Source: UniProtKB
- sulfotransferase activity Source: GO_Central
GO - Biological processi
- peptidyl-tyrosine sulfation Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-tyrosine sulfotransferase 2 (EC:2.8.2.20By similarity)Alternative name(s): Tyrosylprotein sulfotransferase 2 Short name: TPST-2 |
Gene namesi | Name:TPST2 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000010368 |
VGNCi | VGNC:36265, TPST2 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 9 – 25 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 17 | |
Topological domaini | 26 – 377 | LumenalSequence analysisAdd BLAST | 352 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000253723 | 1 – 377 | Protein-tyrosine sulfotransferase 2Add BLAST | 377 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 96 ↔ 156 | By similarity | ||
Disulfide bondi | 225 ↔ 233 | By similarity | ||
Glycosylationi | 343 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 368 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
N-glycosylated.By similarity
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q3SYY2 |
Interactioni
Subunit structurei
Homodimer. Can also form heterodimers with TPST1.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000013685 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 78 – 82 | PAPS bindingBy similarity | 5 | |
Regioni | 101 – 105 | Interaction with peptide substrateBy similarity | 5 | |
Regioni | 285 – 294 | PAPS bindingBy similarity | 10 |
Sequence similaritiesi
Belongs to the protein sulfotransferase family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3988, Eukaryota |
GeneTreei | ENSGT00390000006030 |
InParanoidi | Q3SYY2 |
OMAi | DSNHVEY |
OrthoDBi | 671992at2759 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR026634, TPST |
PANTHERi | PTHR12788, PTHR12788, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q3SYY2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLSMRRALL AAGLALALVL AVHLGQRVLE CQAVLGGPRG PRRTMRPEQE
60 70 80 90 100
DLMMVGADHV EYRYGKAMPL IFVGGVPRSG TTLMRAMLDA HPEVRCGEET
110 120 130 140 150
RIIPRVLAMR QAWSKSGREK LRLDEAGVTD EVLDAAMQAF ILEVIAKHGE
160 170 180 190 200
PARVLCNKDP FTLKSSVYLS RLFPNSKFLL MVRDGRASVH SMITRKVTIA
210 220 230 240 250
GFDLSSYRDC LTKWNKAIEV MYAQCMEVGR DKCLPVYYEQ LVLHPRRSLK
260 270 280 290 300
VILDFLGIAW SDAVLHHEDL IGKPGGVSLS KIERSTDQVI KPVNLEALSK
310 320 330 340 350
WTGHIPGDVL RDMAQIAPML ARLGYDPYAN PPNYGNPDPI VINNTHRVLK
360 370
GDYKTPANLK GYFQVNLNST SSHLGSS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1MIB1 | A0A3Q1MIB1_BOVIN | Protein-tyrosine sulfotransferase | TPST2 | 465 | Annotation score: | ||
A0A3Q1M7S6 | A0A3Q1M7S6_BOVIN | Protein-tyrosine sulfotransferase | TPST2 | 496 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC103334 mRNA Translation: AAI03335.1 |
RefSeqi | NP_001030506.1, NM_001035429.2 |
Genome annotation databases
Ensembli | ENSBTAT00000013685; ENSBTAP00000013685; ENSBTAG00000010368 |
GeneIDi | 540183 |
KEGGi | bta:540183 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC103334 mRNA Translation: AAI03335.1 |
RefSeqi | NP_001030506.1, NM_001035429.2 |
3D structure databases
SMRi | Q3SYY2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000013685 |
Proteomic databases
PaxDbi | Q3SYY2 |
Genome annotation databases
Ensembli | ENSBTAT00000013685; ENSBTAP00000013685; ENSBTAG00000010368 |
GeneIDi | 540183 |
KEGGi | bta:540183 |
Organism-specific databases
CTDi | 8459 |
VEuPathDBi | HostDB:ENSBTAG00000010368 |
VGNCi | VGNC:36265, TPST2 |
Phylogenomic databases
eggNOGi | KOG3988, Eukaryota |
GeneTreei | ENSGT00390000006030 |
InParanoidi | Q3SYY2 |
OMAi | DSNHVEY |
OrthoDBi | 671992at2759 |
Gene expression databases
ExpressionAtlasi | Q3SYY2, baseline and differential |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR026634, TPST |
PANTHERi | PTHR12788, PTHR12788, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TPST2_BOVIN | |
Accessioni | Q3SYY2Primary (citable) accession number: Q3SYY2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | October 11, 2005 | |
Last modified: | September 29, 2021 | |
This is version 80 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families