UniProtKB - Q3SYT8 (NCPR_BOVIN)
Protein
NADPH--cytochrome P450 reductase
Gene
POR
Organism
Bos taurus (Bovine)
Status
Functioni
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation
Catalytic activityi
- EC:1.6.2.4UniRule annotation
Cofactori
Protein has several cofactor binding sites:- FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
- FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 208 | FMNUniRule annotation | 1 | |
Binding sitei | 298 | NADPUniRule annotation | 1 | |
Binding sitei | 424 | FADUniRule annotation | 1 | |
Binding sitei | 478 | FADUniRule annotation | 1 | |
Binding sitei | 535 | NADPUniRule annotation | 1 | |
Binding sitei | 639 | NADPUniRule annotation | 1 | |
Binding sitei | 677 | FADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 86 – 91 | FMNUniRule annotation | 6 | |
Nucleotide bindingi | 138 – 141 | FMNUniRule annotation | 4 | |
Nucleotide bindingi | 173 – 182 | FMNUniRule annotation | 10 | |
Nucleotide bindingi | 454 – 457 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 472 – 474 | FADUniRule annotation | 3 | |
Nucleotide bindingi | 488 – 491 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 596 – 597 | NADPUniRule annotation | 2 | |
Nucleotide bindingi | 602 – 606 | NADPUniRule annotation | 5 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: GO_Central
- FMN binding Source: GO_Central
- NADP binding Source: UniProtKB-UniRule
- NADPH-hemoprotein reductase activity Source: GO_Central
- oxidoreductase activity Source: GO_Central
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: GO_Central
GO - Biological processi
- response to hormone Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, FMN, NADP |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)Short name: CPRUniRule annotation Short name: P450RUniRule annotation |
Gene namesi | Name:PORUniRule annotation |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Cytosol
- cytosol Source: GO_Central
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 21 | LumenalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 22 – 42 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 43 – 678 | CytoplasmicUniRule annotationAdd BLAST | 636 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245573 | 1 – 678 | NADPH--cytochrome P450 reductaseAdd BLAST | 678 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 63 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q3SYT8 |
PeptideAtlasi | Q3SYT8 |
PRIDEi | Q3SYT8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 80 – 224 | Flavodoxin-likeUniRule annotationAdd BLAST | 145 | |
Domaini | 279 – 521 | FAD-binding FR-typeUniRule annotationAdd BLAST | 243 |
Sequence similaritiesi
Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1158, Eukaryota |
HOGENOMi | CLU_001570_17_3_1 |
InParanoidi | Q3SYT8 |
OrthoDBi | 318396at2759 |
Family and domain databases
Gene3Di | 1.20.990.10, 2 hits 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212, NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR001433, OxRdtase_FAD/NAD-bd IPR023208, P450R IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208, P450R, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q3SYT8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADSNMDAGT TTSEMVAEEV SLFSTTDVIL FSLIVGVMTY WFLFRKKKEE
60 70 80 90 100
VPEFTKIQTT TSSVKDRSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK
110 120 130 140 150
DAHRYGMRGM AADPEEYDLA DLSSLPEIEK ALAIFCMATY GEGDPTDNAQ
160 170 180 190 200
DFYDWLQETD VDLSGVKYAV FALGNKTYEH FNAMGKYVDK RLEQLGAQRI
210 220 230 240 250
FDLGLGDDDG NLEEDFITWR EQFWPAVCEH FGVEATGEES SIRQYELMVH
260 270 280 290 300
TDMDMAKVYT GEMGRLKSYE NQKPPFDAKN PFLAVVTTNR KLNQGTERHL
310 320 330 340 350
MHLELDISDS KIRYESGDHV AVYPANDSAL VNQLGEILGA DLDIIMSLNN
360 370 380 390 400
LDEESNKKHP FPCPTSYRTA LTYYLDITNP PRTNVLYELA QYASEPTEHE
410 420 430 440 450
QLRKMASSSG EGKELYLRWV LEARRHILAI LQDYPSLRPP IDHLCELLPR
460 470 480 490 500
LQARYYSIAS SSKVHPNSVH ICAVAVEYET KTGRINKGVA TSWLRAKEPA
510 520 530 540 550
GENGGRALVP MYVRKSQFRL PFKATTPVIM VGPGTGVAPF IGFIQERAWL
560 570 580 590 600
RQQGKEVGET LLYYGCRRSD EDYLYREELA GFHKDGALTQ LNVAFSREQP
610 620 630 640 650
QKVYVQHLLK KDKEHLWKLI HEGGAHIYVC GDARNMARDV QNTFYDIVAE
660 670
QGAMEHAQAV DYVKKLMTKG RYSLDVWS
Sequence cautioni
The sequence AAI03400 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC103399 mRNA Translation: AAI03400.1 Different initiation. |
RefSeqi | NP_001030467.1, NM_001035390.1 |
Genome annotation databases
GeneIDi | 532512 |
KEGGi | bta:532512 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC103399 mRNA Translation: AAI03400.1 Different initiation. |
RefSeqi | NP_001030467.1, NM_001035390.1 |
3D structure databases
SMRi | Q3SYT8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000022718 |
Proteomic databases
PaxDbi | Q3SYT8 |
PeptideAtlasi | Q3SYT8 |
PRIDEi | Q3SYT8 |
Genome annotation databases
GeneIDi | 532512 |
KEGGi | bta:532512 |
Organism-specific databases
CTDi | 5447 |
Phylogenomic databases
eggNOGi | KOG1158, Eukaryota |
HOGENOMi | CLU_001570_17_3_1 |
InParanoidi | Q3SYT8 |
OrthoDBi | 318396at2759 |
Family and domain databases
Gene3Di | 1.20.990.10, 2 hits 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212, NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR001433, OxRdtase_FAD/NAD-bd IPR023208, P450R IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208, P450R, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCPR_BOVIN | |
Accessioni | Q3SYT8Primary (citable) accession number: Q3SYT8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 105 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families