UniProtKB - Q3SYC2 (MOGT2_HUMAN)
Protein
2-acylglycerol O-acyltransferase 2
Gene
MOGAT2
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity. Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).3 Publications
Catalytic activityi
- EC:2.3.1.222 PublicationsThis reaction proceeds in the forwardCurated direction.
- (9Z)-octadecenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 2-(9Z-octadecenoyl)-glycerol + butanoyl-CoA = 1-butyryl-2-(9Z)-octadecenoyl-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 2-(9Z-octadecenoyl)-glycerol + octanoyl-CoA = 1-octanoyl-2-(9Z)-octadecenoyl-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 2-(9Z-octadecenoyl)-glycerol + dodecanoyl-CoA = 1-dodecanoyl-2-(9Z)-octadecenoyl-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 2-(9Z-octadecenoyl)-glycerol + tetradecanoyl-CoA = 1-tetradecanoyl-2-(9Z)-octadecenoyl-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 2-(9Z-octadecenoyl)-glycerol + octadecanoyl-CoA = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 2-(9Z-octadecenoyl)-glycerol + eicosanoyl-CoA = 1-eicosanoyl-2-(9Z)-octadecenoyl-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z,12Z)-octadecadienoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1-(9Z,12Z)-octadecadienoyl-2-(9Z)-octadecenoyl-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-2-(9Z)-octadecenoyl-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-dodecanoylglycerol = 1-dodecanoyl-2-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-tetradecanoylglycerol = 1-tetradecanoyl-2-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-hexadecanoylglycerol = 1-hexadecanoyl-2-(9Z-octadecenoyl)glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-octadecanoylglycerol = 1-octadecanoyl-2-(9Z)-octadecenoylglycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-(9Z,12Z-octadecadienoyl)-glycerol = 1-(9Z,12Z-octadecadienoyl)-2-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-(9Z,12Z,15Z-octadecatrienoyl)-glycerol = 1-(9Z,12Z,15Z-octadecatrienoyl)-2-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-2-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-decanoylglycerol = 1-decanoyl-2-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-glycerol = 1-O-(9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol = 1-O-(9Z-octadecenyl)-2,3-di-(9Z-octadecenoyl)glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Inhibited by oleic acid and sphingosine, while it is stimulated by phosphatidylcholine, phosphatidylserine and phosphatidic acid.By similarity
Kineticsi
- KM=45 µM for sn-1-monooleoylglycerol1 Publication
: triacylglycerol biosynthesis Pathwayi
This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.
GO - Molecular functioni
- 2-acylglycerol O-acyltransferase activity Source: UniProtKB
- acetyltransferase activity Source: Ensembl
- O-acyltransferase activity Source: GO_Central
GO - Biological processi
- diacylglycerol biosynthetic process Source: GO_Central
- glycerol metabolic process Source: UniProtKB-KW
- intestinal absorption Source: Ensembl
- lipid metabolic process Source: GO_Central
- monoacylglycerol biosynthetic process Source: UniProtKB
- triglyceride biosynthetic process Source: Reactome
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Glycerol metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.22, 2681 |
PathwayCommonsi | Q3SYC2 |
Reactomei | R-HSA-75109, Triglyceride biosynthesis |
UniPathwayi | UPA00282 |
Chemistry databases
SwissLipidsi | SLP:000000299 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-acylglycerol O-acyltransferase 2Curated (EC:2.3.1.222 Publications)Alternative name(s): Acyl-CoA:monoacylglycerol acyltransferase 2 Short name: MGAT2 Short name: hMGAT2 Diacylglycerol O-acyltransferase candidate 5 Short name: hDC5 Diacylglycerol acyltransferase 2-like protein 5 Monoacylglycerol O-acyltransferase 2 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:23248, MOGAT2 |
MIMi | 610270, gene |
neXtProti | NX_Q3SYC2 |
VEuPathDBi | HostDB:ENSG00000166391.14 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 1 Publication
Other locations
- perinuclear region 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: Reactome
- perinuclear endoplasmic reticulum membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 23 – 37 | HelicalSequence analysisAdd BLAST | 15 | |
Transmembranei | 38 – 59 | HelicalSequence analysisAdd BLAST | 22 | |
Transmembranei | 103 – 123 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 80168 |
OpenTargetsi | ENSG00000166391 |
PharmGKBi | PA134936582 |
Miscellaneous databases
Pharosi | Q3SYC2, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2439944 |
Genetic variation databases
BioMutai | MOGAT2 |
DMDMi | 114150036 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249062 | 1 – 334 | 2-acylglycerol O-acyltransferase 2Add BLAST | 334 |
Proteomic databases
jPOSTi | Q3SYC2 |
MassIVEi | Q3SYC2 |
PaxDbi | Q3SYC2 |
PeptideAtlasi | Q3SYC2 |
PRIDEi | Q3SYC2 |
ProteomicsDBi | 61855 [Q3SYC2-1] 61856 [Q3SYC2-2] 61857 [Q3SYC2-3] |
PTM databases
iPTMneti | Q3SYC2 |
PhosphoSitePlusi | Q3SYC2 |
Expressioni
Tissue specificityi
Highly expressed in liver, small intestine, colon, stomach and kidney.2 Publications
Gene expression databases
Bgeei | ENSG00000166391, Expressed in small intestine and 80 other tissues |
Genevisiblei | Q3SYC2, HS |
Organism-specific databases
HPAi | ENSG00000166391, Group enriched (intestine, liver) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 123154, 2 interactors |
IntActi | Q3SYC2, 1 interactor |
STRINGi | 9606.ENSP00000198801 |
Chemistry databases
BindingDBi | Q3SYC2 |
Miscellaneous databases
RNActi | Q3SYC2, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the diacylglycerol acyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT00950000182761 |
HOGENOMi | CLU_023995_0_1_1 |
InParanoidi | Q3SYC2 |
OMAi | PFYRDYI |
OrthoDBi | 1347007at2759 |
PhylomeDBi | Q3SYC2 |
TreeFami | TF314707 |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q3SYC2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVEFAPLFMP WERRLQTLAV LQFVFSFLAL AEICTVGFIA LLFTRFWLLT
60 70 80 90 100
VLYAAWWYLD RDKPRQGGRH IQAIRCWTIW KYMKDYFPIS LVKTAELDPS
110 120 130 140 150
RNYIAGFHPH GVLAVGAFAN LCTESTGFSS IFPGIRPHLM MLTLWFRAPF
160 170 180 190 200
FRDYIMSAGL VTSEKESAAH ILNRKGGGNL LGIIVGGAQE ALDARPGSFT
210 220 230 240 250
LLLRNRKGFV RLALTHGAPL VPIFSFGEND LFDQIPNSSG SWLRYIQNRL
260 270 280 290 300
QKIMGISLPL FHGRGVFQYS FGLIPYRRPI TTVVGKPIEV QKTLHPSEEE
310 320 330
VNQLHQRYIK ELCNLFEAHK LKFNIPADQH LEFC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 142 | L → P in BAB15436 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 267 | F → L in BAC87534 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027390 | 9 | M → V1 PublicationCorresponds to variant dbSNP:rs554202Ensembl. | 1 | |
Natural variantiVAR_048857 | 196 | P → H. Corresponds to variant dbSNP:rs34582952Ensembl. | 1 | |
Natural variantiVAR_027391 | 313 | C → Y. Corresponds to variant dbSNP:rs12281468Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020358 | 1 – 82 | Missing in isoform 2. 1 PublicationAdd BLAST | 82 | |
Alternative sequenceiVSP_020359 | 218 – 284 | APLVP…ITTVV → YQASGKSTLGSVGNWQGFYF GGKMAETNADSILVEIFSPF TIKIIFWCLMPKYLEKFPQR RLSDLRN in isoform 3. 1 PublicationAdd BLAST | 67 | |
Alternative sequenceiVSP_020360 | 285 – 334 | Missing in isoform 3. 1 PublicationAdd BLAST | 50 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY157608 mRNA Translation: AAO23672.1 AK026297 mRNA Translation: BAB15436.1 AK128620 mRNA Translation: BAC87534.1 AK291998 mRNA Translation: BAF84687.1 CH471076 Genomic DNA Translation: EAW74983.1 BC103876 mRNA Translation: AAI03877.1 BC103877 mRNA Translation: AAI03878.1 BC103878 mRNA Translation: AAI03879.1 |
CCDSi | CCDS8240.1 [Q3SYC2-1] |
RefSeqi | NP_079374.2, NM_025098.3 [Q3SYC2-1] |
Genome annotation databases
Ensembli | ENST00000198801; ENSP00000198801; ENSG00000166391 [Q3SYC2-1] ENST00000525093; ENSP00000436537; ENSG00000166391 [Q3SYC2-3] ENST00000526712; ENSP00000436283; ENSG00000166391 [Q3SYC2-2] |
GeneIDi | 80168 |
KEGGi | hsa:80168 |
UCSCi | uc010rru.3, human [Q3SYC2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY157608 mRNA Translation: AAO23672.1 AK026297 mRNA Translation: BAB15436.1 AK128620 mRNA Translation: BAC87534.1 AK291998 mRNA Translation: BAF84687.1 CH471076 Genomic DNA Translation: EAW74983.1 BC103876 mRNA Translation: AAI03877.1 BC103877 mRNA Translation: AAI03878.1 BC103878 mRNA Translation: AAI03879.1 |
CCDSi | CCDS8240.1 [Q3SYC2-1] |
RefSeqi | NP_079374.2, NM_025098.3 [Q3SYC2-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 123154, 2 interactors |
IntActi | Q3SYC2, 1 interactor |
STRINGi | 9606.ENSP00000198801 |
Chemistry databases
BindingDBi | Q3SYC2 |
ChEMBLi | CHEMBL2439944 |
SwissLipidsi | SLP:000000299 |
PTM databases
iPTMneti | Q3SYC2 |
PhosphoSitePlusi | Q3SYC2 |
Genetic variation databases
BioMutai | MOGAT2 |
DMDMi | 114150036 |
Proteomic databases
jPOSTi | Q3SYC2 |
MassIVEi | Q3SYC2 |
PaxDbi | Q3SYC2 |
PeptideAtlasi | Q3SYC2 |
PRIDEi | Q3SYC2 |
ProteomicsDBi | 61855 [Q3SYC2-1] 61856 [Q3SYC2-2] 61857 [Q3SYC2-3] |
Protocols and materials databases
Antibodypediai | 31177, 141 antibodies |
Genome annotation databases
Ensembli | ENST00000198801; ENSP00000198801; ENSG00000166391 [Q3SYC2-1] ENST00000525093; ENSP00000436537; ENSG00000166391 [Q3SYC2-3] ENST00000526712; ENSP00000436283; ENSG00000166391 [Q3SYC2-2] |
GeneIDi | 80168 |
KEGGi | hsa:80168 |
UCSCi | uc010rru.3, human [Q3SYC2-1] |
Organism-specific databases
CTDi | 80168 |
DisGeNETi | 80168 |
GeneCardsi | MOGAT2 |
HGNCi | HGNC:23248, MOGAT2 |
HPAi | ENSG00000166391, Group enriched (intestine, liver) |
MIMi | 610270, gene |
neXtProti | NX_Q3SYC2 |
OpenTargetsi | ENSG00000166391 |
PharmGKBi | PA134936582 |
VEuPathDBi | HostDB:ENSG00000166391.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT00950000182761 |
HOGENOMi | CLU_023995_0_1_1 |
InParanoidi | Q3SYC2 |
OMAi | PFYRDYI |
OrthoDBi | 1347007at2759 |
PhylomeDBi | Q3SYC2 |
TreeFami | TF314707 |
Enzyme and pathway databases
UniPathwayi | UPA00282 |
BRENDAi | 2.3.1.22, 2681 |
PathwayCommonsi | Q3SYC2 |
Reactomei | R-HSA-75109, Triglyceride biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 80168, 0 hits in 874 CRISPR screens |
ChiTaRSi | MOGAT2, human |
GeneWikii | MOGAT2 |
GenomeRNAii | 80168 |
Pharosi | Q3SYC2, Tchem |
PROi | PR:Q3SYC2 |
RNActi | Q3SYC2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000166391, Expressed in small intestine and 80 other tissues |
Genevisiblei | Q3SYC2, HS |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MOGT2_HUMAN | |
Accessioni | Q3SYC2Primary (citable) accession number: Q3SYC2 Secondary accession number(s): A8K7I3 Q9H630 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | September 5, 2006 | |
Last modified: | February 10, 2021 | |
This is version 117 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families