UniProtKB - Q3SWY2 (ILK_BOVIN)
Integrin-linked protein kinase
ILK
Functioni
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Regulates cell motility by forming a complex with PARVB. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
By similarityCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 220 | ATPPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 199 – 207 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein kinase activity Source: GO_Central
- protein kinase binding Source: Ensembl
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
GO - Biological processi
- branching involved in ureteric bud morphogenesis Source: Ensembl
- cell-matrix adhesion Source: GO_Central
- cell projection organization Source: Ensembl
- establishment or maintenance of epithelial cell apical/basal polarity Source: Ensembl
- fibroblast migration Source: Ensembl
- integrin-mediated signaling pathway Source: GO_Central
- myelination in peripheral nervous system Source: Ensembl
- negative regulation of neural precursor cell proliferation Source: Ensembl
- nerve development Source: Ensembl
- outflow tract morphogenesis Source: Ensembl
- peptidyl-serine phosphorylation Source: Ensembl
- positive regulation of BMP signaling pathway Source: Ensembl
- positive regulation of canonical Wnt signaling pathway Source: Ensembl
- positive regulation of cell population proliferation Source: Ensembl
- positive regulation of NIK/NF-kappaB signaling Source: Ensembl
- positive regulation of osteoblast differentiation Source: Ensembl
- positive regulation of protein phosphorylation Source: Ensembl
- positive regulation of substrate adhesion-dependent cell spreading Source: Ensembl
- positive regulation of transcription, DNA-templated Source: Ensembl
- protein kinase B signaling Source: Ensembl
- substrate adhesion-dependent cell spreading Source: GO_Central
- tumor necrosis factor-mediated signaling pathway Source: Ensembl
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Integrin-linked protein kinaseBy similarity (EC:2.7.11.1By similarity)Alternative name(s): 59 kDa serine/threonine-protein kinaseBy similarity Beta-integrin-linked kinaseBy similarity ILK-1By similarity ILK-2By similarity p59ILKBy similarity |
Gene namesi | |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000000696 |
VGNCi | VGNC:30177, ILK |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Other locations
- focal adhesion By similarity
- lamellipodium By similarity
- sarcomere By similarity
Nucleus
- nucleoplasm Source: Ensembl
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- focal adhesion Source: GO_Central
- lamellipodium Source: UniProtKB-SubCell
- sarcomere Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000244820 | 1 – 452 | Integrin-linked protein kinaseAdd BLAST | 452 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 186 | PhosphoserineBy similarity | 1 | |
Modified residuei | 426 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q3SWY2 |
PeptideAtlasi | Q3SWY2 |
PRIDEi | Q3SWY2 |
Interactioni
Subunit structurei
Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5.
Interacts (via ANK repeats) with LIMS1 and LIMS2.
Interacts with PARVA (via C-terminus) and PARVB; these compete for the same binding site (By similarity).
Interacts probably also with TGFB1I1 (By similarity).
Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1 (By similarity).
Interacts with FERMT2 (By similarity).
Interacts with LIMD2; leading to activate the protein kinase activity.
Interacts with PXN/PAXILLIN (via LD motif 4).
Interacts with CCDC25 (via cytoplasmic region); initiating the ILK-PARVB cascade to induce cytoskeleton rearrangement and directional migration of cells (By similarity).
By similarityGO - Molecular functioni
- protein kinase binding Source: Ensembl
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000000927 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 2 – 30 | ANK 1Add BLAST | 29 | |
Repeati | 31 – 63 | ANK 2Add BLAST | 33 | |
Repeati | 64 – 96 | ANK 3Add BLAST | 33 | |
Repeati | 97 – 129 | ANK 4Add BLAST | 33 | |
Repeati | 130 – 174 | ANK 5Add BLAST | 45 | |
Domaini | 193 – 446 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 254 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 139 | Interaction with LIMS1By similarityAdd BLAST | 107 | |
Regioni | 180 – 212 | PH-like; mediates interaction with TGFB1I1Add BLAST | 33 |
Domaini
Sequence similaritiesi
Keywords - Domaini
ANK repeat, RepeatPhylogenomic databases
eggNOGi | KOG0195, Eukaryota |
GeneTreei | ENSGT00940000155956 |
InParanoidi | Q3SWY2 |
OMAi | NMADAKF |
Family and domain databases
CDDi | cd14057, PK_ILK, 1 hit |
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR036770, Ankyrin_rpt-contain_sf IPR011009, Kinase-like_dom_sf IPR035692, PK_ILK IPR000719, Prot_kinase_dom IPR001245, Ser-Thr/Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF12796, Ank_2, 2 hits PF07714, PK_Tyr_Ser-Thr, 1 hit |
SMARTi | View protein in SMART SM00248, ANK, 3 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 3 hits PS50011, PROTEIN_KINASE_DOM, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV
60 70 80 90 100
EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG
110 120 130 140 150
NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KPPLRELLRE
160 170 180 190 200
RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLAKLN
210 220 230 240 250
ENHSGELWKG RWQGNDIVVK MLKVRDWSTR KSRDFNEECP RLRIFSHPNV
260 270 280 290 300
LPVLGACQSP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD
310 320 330 340 350
MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM
360 370 380 390 400
YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI
410 420 430 440 450
GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ
DK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC104603 mRNA Translation: AAI04604.1 |
RefSeqi | NP_001029865.1, NM_001034693.1 XP_005216186.1, XM_005216129.3 XP_005216187.1, XM_005216130.2 XP_005216188.1, XM_005216131.1 |
Genome annotation databases
Ensembli | ENSBTAT00000000927; ENSBTAP00000000927; ENSBTAG00000000696 |
GeneIDi | 540207 |
KEGGi | bta:540207 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC104603 mRNA Translation: AAI04604.1 |
RefSeqi | NP_001029865.1, NM_001034693.1 XP_005216186.1, XM_005216129.3 XP_005216187.1, XM_005216130.2 XP_005216188.1, XM_005216131.1 |
3D structure databases
AlphaFoldDBi | Q3SWY2 |
BMRBi | Q3SWY2 |
SMRi | Q3SWY2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000000927 |
Proteomic databases
PaxDbi | Q3SWY2 |
PeptideAtlasi | Q3SWY2 |
PRIDEi | Q3SWY2 |
Genome annotation databases
Ensembli | ENSBTAT00000000927; ENSBTAP00000000927; ENSBTAG00000000696 |
GeneIDi | 540207 |
KEGGi | bta:540207 |
Organism-specific databases
CTDi | 3611 |
VEuPathDBi | HostDB:ENSBTAG00000000696 |
VGNCi | VGNC:30177, ILK |
Phylogenomic databases
eggNOGi | KOG0195, Eukaryota |
GeneTreei | ENSGT00940000155956 |
InParanoidi | Q3SWY2 |
OMAi | NMADAKF |
Gene expression databases
ExpressionAtlasi | Q3SWY2, baseline |
Family and domain databases
CDDi | cd14057, PK_ILK, 1 hit |
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR036770, Ankyrin_rpt-contain_sf IPR011009, Kinase-like_dom_sf IPR035692, PK_ILK IPR000719, Prot_kinase_dom IPR001245, Ser-Thr/Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF12796, Ank_2, 2 hits PF07714, PK_Tyr_Ser-Thr, 1 hit |
SMARTi | View protein in SMART SM00248, ANK, 3 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 3 hits PS50011, PROTEIN_KINASE_DOM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ILK_BOVIN | |
Accessioni | Q3SWY2Primary (citable) accession number: Q3SWY2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | October 11, 2005 | |
Last modified: | May 25, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families