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Protein

RISC-loading complex subunit TARBP2

Gene

Tarbp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processRNA-mediated gene silencing, Translation regulation

Enzyme and pathway databases

ReactomeiR-RNO-203927 MicroRNA (miRNA) biogenesis
R-RNO-426486 Small interfering RNA (siRNA) biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
RISC-loading complex subunit TARBP2UniRule annotation
Gene namesi
Name:Tarbp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1307375 Tarbp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003739711 – 365RISC-loading complex subunit TARBP2Add BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei151PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3SWU0
PRIDEiQ3SWU0

Expressioni

Gene expression databases

BgeeiENSRNOG00000042355 Expressed in 10 organ(s), highest expression level in heart
GenevisibleiQ3SWU0 RN

Interactioni

Subunit structurei

Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9 and PRKRA. Interacts with DICER1, AGO2, MOV10, EIF6 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC).UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061329

Structurei

3D structure databases

ProteinModelPortaliQ3SWU0
SMRiQ3SWU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 97DRBM 1UniRule annotationAdd BLAST68
Domaini158 – 226DRBM 2UniRule annotationAdd BLAST69
Domaini292 – 360DRBM 3UniRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 105Sufficient for interaction with PRKRAUniRule annotationAdd BLAST84
Regioni151 – 233Sufficient for interaction with PRKRAUniRule annotationAdd BLAST83
Regioni227 – 365Sufficient for interaction with DICER1UniRule annotationAdd BLAST139
Regioni286 – 365Sufficient for interaction with PRKRAUniRule annotationAdd BLAST80

Sequence similaritiesi

Belongs to the TARBP2 family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3732 Eukaryota
ENOG410XSCK LUCA
GeneTreeiENSGT00900000141030
HOGENOMiHOG000231919
InParanoidiQ3SWU0
KOiK18420
OMAiQPITVCH
OrthoDBiEOG091G0I2L
PhylomeDBiQ3SWU0

Family and domain databases

CDDicd00048 DSRM, 2 hits
HAMAPiMF_03034 TRBP2, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR028605 TRBP2
PANTHERiPTHR10910:SF61 PTHR10910:SF61, 1 hit
PfamiView protein in Pfam
PF00035 dsrm, 2 hits
SMARTiView protein in SMART
SM00358 DSRM, 3 hits
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 3 hits

Sequencei

Sequence statusi: Complete.

Q3SWU0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEEDQGSGS TTGCGLPSIE QMLAANPGKT PISLLQEYGT RIGKTPVYDL
60 70 80 90 100
LKAEGQAHQP NFTFRVTVGD TSCTGQGPSK KAAKHKAAEV ALKHLKGGSM
110 120 130 140 150
LEPALEDSSS FSLLDSSVPE DTAVIAAEAA APVPSALLTR SPPMEMQPPV
160 170 180 190 200
SPQQSECNPV GALQELVVQK GWRLPEYMVT QESGPAHRKE FTMTCRVERF
210 220 230 240 250
IEIGSGTSKK LAKRNAAAKM LLRVHTVPLD ARDGNEAEPD DDHFSIGVSS
260 270 280 290 300
RLDGLRNRGP GCTWDSLRNS VGEKILSLRN CSVGSLGSLG SACCSILSEL
310 320 330 340 350
SAEQAFHVSY LDIEELSLSG LCQCLVELST QPATVCYGSA TTREAARGDA
360
ARRALQYLRI MAGSK
Length:365
Mass (Da):38,850
Last modified:October 11, 2005 - v1
Checksum:i5027E6C492840EAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474035 mRNA Translation: EDL86826.1
BC104690 mRNA Translation: AAI04691.1
RefSeqiNP_001030113.1, NM_001034941.1
UniGeneiRn.17227

Genome annotation databases

EnsembliENSRNOT00000064661; ENSRNOP00000061329; ENSRNOG00000042355
GeneIDi363006
KEGGirno:363006
UCSCiRGD:1307375 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474035 mRNA Translation: EDL86826.1
BC104690 mRNA Translation: AAI04691.1
RefSeqiNP_001030113.1, NM_001034941.1
UniGeneiRn.17227

3D structure databases

ProteinModelPortaliQ3SWU0
SMRiQ3SWU0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061329

Proteomic databases

PaxDbiQ3SWU0
PRIDEiQ3SWU0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000064661; ENSRNOP00000061329; ENSRNOG00000042355
GeneIDi363006
KEGGirno:363006
UCSCiRGD:1307375 rat

Organism-specific databases

CTDi6895
RGDi1307375 Tarbp2

Phylogenomic databases

eggNOGiKOG3732 Eukaryota
ENOG410XSCK LUCA
GeneTreeiENSGT00900000141030
HOGENOMiHOG000231919
InParanoidiQ3SWU0
KOiK18420
OMAiQPITVCH
OrthoDBiEOG091G0I2L
PhylomeDBiQ3SWU0

Enzyme and pathway databases

ReactomeiR-RNO-203927 MicroRNA (miRNA) biogenesis
R-RNO-426486 Small interfering RNA (siRNA) biogenesis

Miscellaneous databases

PROiPR:Q3SWU0

Gene expression databases

BgeeiENSRNOG00000042355 Expressed in 10 organ(s), highest expression level in heart
GenevisibleiQ3SWU0 RN

Family and domain databases

CDDicd00048 DSRM, 2 hits
HAMAPiMF_03034 TRBP2, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR028605 TRBP2
PANTHERiPTHR10910:SF61 PTHR10910:SF61, 1 hit
PfamiView protein in Pfam
PF00035 dsrm, 2 hits
SMARTiView protein in SMART
SM00358 DSRM, 3 hits
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTRBP2_RAT
AccessioniPrimary (citable) accession number: Q3SWU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: October 11, 2005
Last modified: November 7, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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