UniProtKB - Q3MII6 (TBC25_HUMAN)
Protein
TBC1 domain family member 25
Gene
TBC1D25
Organism
Homo sapiens (Human)
Status
Functioni
Acts as a GTPase-activating protein specific for RAB33B. Involved in the regulation of autophagosome maturation, the process in which autophagosomes fuse with endosomes and lysosomes.1 Publication
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB
GO - Biological processi
- activation of GTPase activity Source: GO_Central
- autophagy Source: UniProtKB-KW
- intracellular protein transport Source: GO_Central
- regulation of autophagosome maturation Source: UniProtKB
Keywordsi
Molecular function | GTPase activation |
Biological process | Autophagy |
Enzyme and pathway databases
PathwayCommonsi | Q3MII6 |
Reactomei | R-HSA-8854214, TBC/RABGAPs |
SIGNORi | Q3MII6 |
Names & Taxonomyi
Protein namesi | Recommended name: TBC1 domain family member 25 |
Gene namesi | Name:TBC1D25 Synonyms:OATL1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8092, TBC1D25 |
MIMi | 311240, gene |
neXtProti | NX_Q3MII6 |
VEuPathDBi | HostDB:ENSG00000068354.15 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- autophagosome 1 Publication
Note: It is dispersed in the cytoplasm under nutrient-rich conditions. Localizes at autophagosomes under cell starving conditions.
Other locations
- autophagosome Source: UniProtKB
- cytoplasmic vesicle Source: UniProtKB-KW
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesiclePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 134 – 135 | ED → AA: Severely affects interaction with GABARAP. Does not localize at autophagosomes. 1 Publication | 2 | |
Mutagenesisi | 136 | W → A: Abolishes interaction with GABARAP. Does not localize at autophagosomes. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 4943 |
OpenTargetsi | ENSG00000068354 |
PharmGKBi | PA162405201 |
Miscellaneous databases
Pharosi | Q3MII6, Tbio |
Genetic variation databases
BioMutai | TBC1D25 |
DMDMi | 296452922 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000288508 | 1 – 688 | TBC1 domain family member 25Add BLAST | 688 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 140 | PhosphoserineBy similarity | 1 | |
Modified residuei | 160 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 506 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q3MII6 |
jPOSTi | Q3MII6 |
MassIVEi | Q3MII6 |
MaxQBi | Q3MII6 |
PaxDbi | Q3MII6 |
PeptideAtlasi | Q3MII6 |
PRIDEi | Q3MII6 |
ProteomicsDBi | 61786 [Q3MII6-1] 61787 [Q3MII6-2] |
PTM databases
iPTMneti | Q3MII6 |
PhosphoSitePlusi | Q3MII6 |
Expressioni
Gene expression databases
Bgeei | ENSG00000068354, Expressed in heart left ventricle and 169 other tissues |
ExpressionAtlasi | Q3MII6, baseline and differential |
Genevisiblei | Q3MII6, HS |
Organism-specific databases
HPAi | ENSG00000068354, Low tissue specificity |
Interactioni
Subunit structurei
Interacts (via N-terminus) with MAP1LC3B, GABARAP and GABARAPL2.
1 PublicationBinary interactionsi
Hide detailsQ3MII6
Protein-protein interaction databases
BioGRIDi | 110997, 52 interactors |
IntActi | Q3MII6, 17 interactors |
STRINGi | 9606.ENSP00000365962 |
Miscellaneous databases
RNActi | Q3MII6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 228 – 434 | Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST | 207 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 22 – 28 | Poly-Ala | 7 | |
Compositional biasi | 29 – 34 | Poly-Glu | 6 | |
Compositional biasi | 494 – 499 | Poly-Gly | 6 | |
Compositional biasi | 559 – 564 | Poly-Ser | 6 |
Phylogenomic databases
eggNOGi | KOG2197, Eukaryota |
GeneTreei | ENSGT00940000159173 |
HOGENOMi | CLU_004382_1_0_1 |
InParanoidi | Q3MII6 |
OMAi | VKVKKCD |
OrthoDBi | 1495285at2759 |
PhylomeDBi | Q3MII6 |
TreeFami | TF323518 |
Family and domain databases
InterProi | View protein in InterPro IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf |
Pfami | View protein in Pfam PF00566, RabGAP-TBC, 1 hit |
SMARTi | View protein in SMART SM00164, TBC, 1 hit |
SUPFAMi | SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50086, TBC_RABGAP, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q3MII6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATASGASDL SGSGAPPPGV GAQAAAAAEE EEREVVRVRV KKCESFLPPE
60 70 80 90 100
FRSFAVDPQI TSLDVLQHIL IRAFDLSGKK NFGISYLGRD RLGQEVYLSL
110 120 130 140 150
LSDWDLSTAF ATASKPYLQL RVDIRPSEDS PLLEDWDIIS PKDVIGSDVL
160 170 180 190 200
LAEKRSSLTT AALPFTQSIL TQVGRTLSKV QQVLSWSYGE DVKPFKPPLS
210 220 230 240 250
DAEFHTYLNH EGQLSRPEEL RLRIYHGGVE PSLRKVVWRY LLNVYPDGLT
260 270 280 290 300
GRERMDYMKR KSREYEQLKS EWAQRANPED LEFIRSTVLK DVLRTDRAHP
310 320 330 340 350
YYAGPEDGPH LRALHDLLTT YAVTHPQVSY CQGMSDLASP ILAVMDHEGH
360 370 380 390 400
AFVCFCGIMK RLAANFHPDG RAMATKFAHL KLLLRHADPD FYQYLQEAGA
410 420 430 440 450
DDLFFCYRWL LLELKREFAF DDALRMLEVT WSSLPPDPPE HEVELVGPPS
460 470 480 490 500
QVADAGFGGH RGWPVRQRHM LRPAGGGGST FEDAVDHLAT ASQGPGGGGR
510 520 530 540 550
LLRQASLDGL QQLRDNMGSR RDPLVQLPHP AALISSKSLS EPLLNSPDPL
560 570 580 590 600
LSSFSHPDSP SSSSPPSTQE ASPTGDMAVG SPLMQEVGSP KDPGKSLPPV
610 620 630 640 650
PPMGLPPPQE FGRGNPFMLF LCLAILLEHR DHIMRNGLDY NELAMHFDRL
660 670 680
VRKHHLGRVL RRARALFADY LQSEVWDSEE GAEATAAS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9JMI8 | C9JMI8_HUMAN | TBC1 domain family member 25 | TBC1D25 | 230 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 455 | A → T in AAI01818 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 455 | A → T in AAI01820 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 455 | A → T in AAI25089 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_057345 | 277 | N → S1 PublicationCorresponds to variant dbSNP:rs2293948Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_025703 | 79 – 100 | KKNFG…VYLSL → PIARRLGHNQPQRCHWLRRV AG in isoform 2. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_025704 | 101 – 688 | Missing in isoform 2. 1 PublicationAdd BLAST | 588 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC115618 Genomic DNA No translation available. AF196969 Genomic DNA No translation available. BC026050 mRNA Translation: AAH26050.1 BC101817 mRNA Translation: AAI01818.1 BC101819 mRNA Translation: AAI01820.1 BC125088 mRNA Translation: AAI25089.1 BC125089 mRNA Translation: AAI25090.2 |
CCDSi | CCDS35242.1 [Q3MII6-1] |
RefSeqi | NP_001335191.1, NM_001348262.1 NP_002527.1, NM_002536.3 [Q3MII6-1] |
Genome annotation databases
Ensembli | ENST00000376771; ENSP00000365962; ENSG00000068354 [Q3MII6-1] ENST00000481090; ENSP00000476787; ENSG00000068354 [Q3MII6-2] |
GeneIDi | 4943 |
KEGGi | hsa:4943 |
UCSCi | uc004dka.2, human [Q3MII6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC115618 Genomic DNA No translation available. AF196969 Genomic DNA No translation available. BC026050 mRNA Translation: AAH26050.1 BC101817 mRNA Translation: AAI01818.1 BC101819 mRNA Translation: AAI01820.1 BC125088 mRNA Translation: AAI25089.1 BC125089 mRNA Translation: AAI25090.2 |
CCDSi | CCDS35242.1 [Q3MII6-1] |
RefSeqi | NP_001335191.1, NM_001348262.1 NP_002527.1, NM_002536.3 [Q3MII6-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 110997, 52 interactors |
IntActi | Q3MII6, 17 interactors |
STRINGi | 9606.ENSP00000365962 |
PTM databases
iPTMneti | Q3MII6 |
PhosphoSitePlusi | Q3MII6 |
Genetic variation databases
BioMutai | TBC1D25 |
DMDMi | 296452922 |
Proteomic databases
EPDi | Q3MII6 |
jPOSTi | Q3MII6 |
MassIVEi | Q3MII6 |
MaxQBi | Q3MII6 |
PaxDbi | Q3MII6 |
PeptideAtlasi | Q3MII6 |
PRIDEi | Q3MII6 |
ProteomicsDBi | 61786 [Q3MII6-1] 61787 [Q3MII6-2] |
Protocols and materials databases
Antibodypediai | 54837, 40 antibodies |
Genome annotation databases
Ensembli | ENST00000376771; ENSP00000365962; ENSG00000068354 [Q3MII6-1] ENST00000481090; ENSP00000476787; ENSG00000068354 [Q3MII6-2] |
GeneIDi | 4943 |
KEGGi | hsa:4943 |
UCSCi | uc004dka.2, human [Q3MII6-1] |
Organism-specific databases
CTDi | 4943 |
DisGeNETi | 4943 |
GeneCardsi | TBC1D25 |
HGNCi | HGNC:8092, TBC1D25 |
HPAi | ENSG00000068354, Low tissue specificity |
MIMi | 311240, gene |
neXtProti | NX_Q3MII6 |
OpenTargetsi | ENSG00000068354 |
PharmGKBi | PA162405201 |
VEuPathDBi | HostDB:ENSG00000068354.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2197, Eukaryota |
GeneTreei | ENSGT00940000159173 |
HOGENOMi | CLU_004382_1_0_1 |
InParanoidi | Q3MII6 |
OMAi | VKVKKCD |
OrthoDBi | 1495285at2759 |
PhylomeDBi | Q3MII6 |
TreeFami | TF323518 |
Enzyme and pathway databases
PathwayCommonsi | Q3MII6 |
Reactomei | R-HSA-8854214, TBC/RABGAPs |
SIGNORi | Q3MII6 |
Miscellaneous databases
BioGRID-ORCSi | 4943, 3 hits in 622 CRISPR screens |
ChiTaRSi | TBC1D25, human |
GenomeRNAii | 4943 |
Pharosi | Q3MII6, Tbio |
PROi | PR:Q3MII6 |
RNActi | Q3MII6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000068354, Expressed in heart left ventricle and 169 other tissues |
ExpressionAtlasi | Q3MII6, baseline and differential |
Genevisiblei | Q3MII6, HS |
Family and domain databases
InterProi | View protein in InterPro IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf |
Pfami | View protein in Pfam PF00566, RabGAP-TBC, 1 hit |
SMARTi | View protein in SMART SM00164, TBC, 1 hit |
SUPFAMi | SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50086, TBC_RABGAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TBC25_HUMAN | |
Accessioni | Q3MII6Primary (citable) accession number: Q3MII6 Secondary accession number(s): Q08AN9, Q3MII4, Q8TAR9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | May 18, 2010 | |
Last modified: | April 7, 2021 | |
This is version 123 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot