UniProtKB - Q3MID2 (CP4F3_RAT)
Cytochrome P450 4F3
Cyp4f3
Functioni
A cytochrome P450 monooxygenase involved in the metabolism of the pro-inflammatory lipid mediator leukotriene B4 (LTB4). Hydroxylates at the omega-1 and omega-2 positions LTB4. This oxidation step leads to LTB4 inactivation, which is postulated to be a crucial part of the resolution of inflammation. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
By similarityCatalytic activityi
- leukotriene B4 + O2 + reduced [NADPH—hemoprotein reductase] = 18-hydroxy-leukotriene B4 + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- leukotriene B4 + O2 + reduced [NADPH—hemoprotein reductase] = 19-hydroxy-leukotriene B4 + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
: leukotriene B4 degradation Pathwayi
This protein is involved in the pathway leukotriene B4 degradation, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway leukotriene B4 degradation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 468 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- arachidonic acid monooxygenase activity Source: GO_Central
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- leukotriene-B4 20-monooxygenase activity Source: GO_Central
- long-chain fatty acid omega-1 hydroxylase activity Source: UniProtKB
GO - Biological processi
- arachidonic acid metabolic process Source: GO_Central
- leukotriene B4 catabolic process Source: GO_Central
- leukotriene B4 metabolic process Source: UniProtKB
- menaquinone catabolic process Source: GO_Central
- phylloquinone catabolic process Source: GO_Central
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Lipid metabolism |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-211935, Fatty acids R-RNO-211958, Miscellaneous substrates R-RNO-211979, Eicosanoids R-RNO-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-RNO-2142816, Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE) |
SABIO-RKi | Q3MID2 |
UniPathwayi | UPA00883 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 4F3By similarityAlternative name(s): CYPIVF3 Leukotriene-B(4) omega-1/omega-2 hydroxylaseBy similarity |
Gene namesi | Name:Cyp4f3By similarity Synonyms:Cyp4f18Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1305261, Cyp4f18 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Curated
- Microsome membrane By similarity; Single-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 15 – 35 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000238925 | 1 – 524 | Cytochrome P450 4F3Add BLAST | 524 |
Proteomic databases
PaxDbi | Q3MID2 |
PRIDEi | Q3MID2 |
PTM databases
iPTMneti | Q3MID2 |
PhosphoSitePlusi | Q3MID2 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0157, Eukaryota |
InParanoidi | Q3MID2 |
OrthoDBi | 1247045at2759 |
PhylomeDBi | Q3MID2 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MPLLSLSWLG LGHTAASPWL LLLLVGASCL LAYILPQVYA VFENSRRLRR
60 70 80 90 100
FPQPPPRNWL FGHLGLIQSS EEGLLYIQSL SRTFRDVCCW WVGPWHPVIR
110 120 130 140 150
IFHPAFIKPV ILAPASVAPK DRVFYRFLRP WLGDGLLLST GDKWSRHRRM
160 170 180 190 200
LTPAFHFNIL KPYVKIFNDS TNIMHAKWQR LASQGSARLD MFEHISLMTL
210 220 230 240 250
DSLQKCVFSF DSNCQEKPSE YITAILELSA LVARRHQSLL LHVDLFYHLT
260 270 280 290 300
RDGMRFRKAC RLVHDFTDAV IRERRCTLPD QGGDDALKAK AKAKTLDFID
310 320 330 340 350
VLLLSKDEHG EALSDEDIRA EADTFMFGGH DTTASGLSWI LYNLAKHPEY
360 370 380 390 400
QERCRQEVRE LLRDREPEEI EWDDLAQLPF LTMCIKESLR LHPPATAISR
410 420 430 440 450
CCTQDIMLPD GRVIPKGVIC RISIFGTHHN PAVWPDPEVY NPFRFDADNG
460 470 480 490 500
KGRSPLAFIP FSAGPRNCIG QTFAMSEMKV ALALTLLRFR VLPDDKEPRR
510 520
KPELILRAEG GLWLRVEPLS AGAH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1M7K8 | F1M7K8_RAT | Cytochrome P450, family 4, subfamil... | Cyp4f18 | 524 | Annotation score: | ||
A0A0G2K1T1 | A0A0G2K1T1_RAT | Cytochrome P450, family 4, subfamil... | Cyp4f18 | 486 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC101918 mRNA Translation: AAI01919.1 |
RefSeqi | NP_001028858.1, NM_001033686.1 |
Genome annotation databases
GeneIDi | 290623 |
KEGGi | rno:290623 |
UCSCi | RGD:1305261, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC101918 mRNA Translation: AAI01919.1 |
RefSeqi | NP_001028858.1, NM_001033686.1 |
3D structure databases
AlphaFoldDBi | Q3MID2 |
SMRi | Q3MID2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000021609 |
PTM databases
iPTMneti | Q3MID2 |
PhosphoSitePlusi | Q3MID2 |
Proteomic databases
PaxDbi | Q3MID2 |
PRIDEi | Q3MID2 |
Genome annotation databases
GeneIDi | 290623 |
KEGGi | rno:290623 |
UCSCi | RGD:1305261, rat |
Organism-specific databases
CTDi | 72054 |
RGDi | 1305261, Cyp4f18 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
InParanoidi | Q3MID2 |
OrthoDBi | 1247045at2759 |
PhylomeDBi | Q3MID2 |
Enzyme and pathway databases
UniPathwayi | UPA00883 |
Reactomei | R-RNO-211935, Fatty acids R-RNO-211958, Miscellaneous substrates R-RNO-211979, Eicosanoids R-RNO-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-RNO-2142816, Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE) |
SABIO-RKi | Q3MID2 |
Miscellaneous databases
PROi | PR:Q3MID2 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP4F3_RAT | |
Accessioni | Q3MID2Primary (citable) accession number: Q3MID2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2006 |
Last sequence update: | October 25, 2005 | |
Last modified: | May 25, 2022 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families