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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

MTAP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation1 Publication

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (MTAP), S-methyl-5'-thioadenosine phosphorylase (MTAP)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei18PhosphateUniRule annotation1
Sitei178Important for substrate specificityUniRule annotation1
Binding sitei196Substrate; via amide nitrogenUniRule annotation1
Binding sitei197PhosphateUniRule annotation1
Sitei233Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

UniPathwayi
UPA00904;UER00873

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
Name:MTAPUniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004151121 – 283S-methyl-5'-thioadenosine phosphorylaseAdd BLAST283

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ3MHF7
PRIDEiQ3MHF7

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000045533

Structurei

3D structure databases

ProteinModelPortaliQ3MHF7
SMRiQ3MHF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 61Phosphate bindingUniRule annotation2
Regioni93 – 94Phosphate bindingUniRule annotation2
Regioni220 – 222Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3985 Eukaryota
COG0005 LUCA
HOGENOMiHOG000228986
HOVERGENiHBG002487
InParanoidiQ3MHF7
KOiK00772

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q3MHF7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSGATPAAV KIGIIGGTGL DDPEILEGRT EKYVDTPFGK PSDALVLGKI
60 70 80 90 100
KNVDCVLLAR HGRQHTIMPS KVNYQANIWA LKEEGCTHVI VTTACGSLKE
110 120 130 140 150
EIQPGDIIII DQFIDRTTRR LQTFYDGNHS CARGVCHIPM AEPFCPKTRE
160 170 180 190 200
VLIETAKKLG LRCHSKGTMI TIEGPRFSSR AESIMFQTWG ADVINMTTVP
210 220 230 240 250
EVVLAKEAGI CYASIAMATD YDCWKEHEEA VSVDRVLKTL KENANKAKSL
260 270 280
LLTTIPQIGS MEWSETLHNM KKMAQFSVLL PRH
Length:283
Mass (Da):31,256
Last modified:October 25, 2005 - v1
Checksum:i9618D53A124CA360
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02022238 Genomic DNA No translation available.
BC105254 mRNA Translation: AAI05255.1
RefSeqiXP_002689552.1, XM_002689506.5
XP_015319924.1, XM_015464438.1
UniGeneiBt.103308
Bt.62827

Genome annotation databases

GeneIDi782907
KEGGibta:782907

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02022238 Genomic DNA No translation available.
BC105254 mRNA Translation: AAI05255.1
RefSeqiXP_002689552.1, XM_002689506.5
XP_015319924.1, XM_015464438.1
UniGeneiBt.103308
Bt.62827

3D structure databases

ProteinModelPortaliQ3MHF7
SMRiQ3MHF7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000045533

Proteomic databases

PaxDbiQ3MHF7
PRIDEiQ3MHF7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi782907
KEGGibta:782907

Organism-specific databases

CTDi4507

Phylogenomic databases

eggNOGiKOG3985 Eukaryota
COG0005 LUCA
HOGENOMiHOG000228986
HOVERGENiHBG002487
InParanoidiQ3MHF7
KOiK00772

Enzyme and pathway databases

UniPathwayi
UPA00904;UER00873

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMTAP_BOVIN
AccessioniPrimary (citable) accession number: Q3MHF7
Secondary accession number(s): F1N1J3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 25, 2005
Last modified: October 10, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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